pycom08g02630

Domain of unknown function (DUF4091)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008088
Physical Location & Seq
Reverse (-)
1938627 .. 1941694
3068 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1299 bp
ATGGTTGAGCCCATTGATGGGAAACCGGTGGATGAAGTGGTAGAAGGGGTGAAATCTGCTACGACATCTTTGAGAAGAGTTCTTCTGTCGCCATTATTTTCTGAATTCTGTACATTAAATGGGCCAGTTGATATGATGGAGGAAGATGCTGTTTCGAGCCTTTCAGCACGTGTGAAGATTAATATGACAGTTTGGGACTTCATTCTTCCTGCAACCCCATCACTTCCTGCTGTTTTTGGCATATCTGATACTGTAATTGAAGATCGTTTTGGTGTTGAACATGGGACTGATGAGTGGTATGAGGCACTGGATCAGCATTTTAAGTGGCTTCTTCAATACAAAATCAGTCCATACTTTTGCAGATGGGGTGATAGTATGCGTGTCTTGACGTATACCTGCCCTTGGCCAGCTGATCATCCAAAATCAGATGAATACTTTTCGGATCCACGGTTGGCAGCCTATGCTTTGCCATATAGTCGATCTGTCTCTGGTGGTGATGCAGCGAAGGATTACATGCAGAAACAAATTGATATATTGAGGACAAAGTCTCACTGGAAGAAAGCATACTTTTACTTGTGGGATGAGCCACTGAATCTGGAGCAATATGAGTCCCTCCGCAGCTTAGCCAGCGAGATCCATGCGTATGCGCCTGATGCTCGTGTTTTAACTACTTACTACTGTGGGCCTAGCAATGCACCTACTGCGTTTGAGGGTTTTGTGAAAGTTCCAAAATTCCTGCGCCCACATACTCAAATTTATTGTACAAGTGAATGGGTGCTTGGGAATCGAGAGGATTTGGTCAAAGATATTGTTTCCGAAATACAAACAGAAAATGGCGAGGAATGGTGGACTTACGTGTGCTTGGGACCGTCTGACCCCCATCCCAATTGGCACCTTGGGATGCGAGGTACGCAACACCGTGCTGTAATGTGGCGTGTATGGAAAGAAGGTGGAACAGGTTTCTTATATTGGGGTGCCAATTGCTACGAGAAGGCAAATGTTCCAAGCGCAGAGATAAGATTCAGGCGTGGCCTTCCCCCCGGAGATGGAGTTTTGTTCTACCCTGGCCAGGTGTTCTCATCATCAAGTCAACCAGTTGCTTCGGTCAGACTCGAGCGCATTCTCAGTGGCTTGCAGGACATTGAGTACCTCAGACTCTACTCTTCAAGATACGGTCGAGATGAAGGACTGGCTCTCCTGGAAAAGACGGGTTTGTACCTCGGTCCTGAGCGTTACACGCACGATCACACGCCCATTGATGCAATGCGGGGTGAGATCTACAATGCATGTCGCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4091

Protein Analysis

433

Amino Acids

49.36

Weight (kDa)

5.4

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH123_cat PF13320 167 - 386 2.6e-15 Glycoside hydrolase 123, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 404
AccB1I GGYRCC 2 cut(s) 891, 974
AccI GTMKAC 1 cut(s) 392
AciI CCGC 2 cut(s) 616, 1267
AclWI GGATC 4 cut(s) 318, 437, 450, 628
AcoI YGGCCR 2 cut(s) 404, 1066
AcsI RAATTY 3 cut(s) 104, 731, 753
AcvI CACGTG 1 cut(s) 170
AfaI GTAC 5 cut(s) 112, 763, 910, 1148, 1217
AfiI CCNNNNNNNGG 5 cut(s) 17, 18, 402, 1046, 1069
AflIII ACRYGT 2 cut(s) 169, 855
AgeI ACCGGT 1 cut(s) 25
AgsI TTSAA 4 cut(s) 260, 278, 335, 1167
AjnI CCWGG 3 cut(s) 1063, 1068, 1197
AjuI GAANNNNNNNTTGG 4 cut(s) 252, 284, 762, 794
AluBI AGCT 2 cut(s) 410, 621
AluI AGCT 2 cut(s) 410, 621
Alw26I GTCTC 2 cut(s) 490, 552
AlwI GGATC 4 cut(s) 318, 437, 450, 628
Ama87I CYCGRG 1 cut(s) 1112
AoxI GGCC 5 cut(s) 122, 404, 683, 1030, 1066
ApeKI GCWGC 3 cut(s) 455, 500, 618
ApoI RAATTY 3 cut(s) 104, 731, 753
AseI ATTAAT 1 cut(s) 180
AsiGI ACCGGT 1 cut(s) 25
AspLEI GCGC 4 cut(s) 649, 741, 1010, 1119
AspS9I GGNCC 4 cut(s) 122, 683, 866, 1223
AsuC2I CCSGG 1 cut(s) 1041
AsuHPI GGTGA 4 cut(s) 61, 380, 506, 1283
AvaI CYCGRG 1 cut(s) 1112
AvaII GGWCC 2 cut(s) 866, 1223
BalI TGGCCA 2 cut(s) 406, 1068
BamHI GGATCC 1 cut(s) 442
BanI GGYRCC 2 cut(s) 891, 974
BanII GRGCYC 1 cut(s) 12
BarI GAAGNNNNNNTAC 2 cut(s) 548, 580
BauI CACGAG 2 cut(s) 657, 1294
BbrPI CACGTG 1 cut(s) 170
BbvI GCAGC 3 cut(s) 467, 512, 630
BccI CCATC 6 cut(s) 11, 130, 226, 357, 888, 1040
BcgI CGANNNNNNTGC 3 cut(s) 620, 654, 1275
BciT130I CCWGG 3 cut(s) 1065, 1070, 1199
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 1 cut(s) 1041
BcoDI GTCTC 2 cut(s) 490, 552
BfaI CTAG 1 cut(s) 687
BfuAI ACCTGC 1 cut(s) 404
BglII AGATCT 1 cut(s) 1275
BisI GCNGC 3 cut(s) 456, 501, 619
BlpI GCTNAGC 1 cut(s) 622
BlsI GCNGC 3 cut(s) 457, 502, 620
Bme1390I CCNGG 4 cut(s) 1041, 1065, 1070, 1199
Bme18I GGWCC 2 cut(s) 866, 1223
BmeT110I CYCGRG 1 cut(s) 1112
BmgT120I GGNCC 4 cut(s) 122, 683, 866, 1223
BmiI GGNNCC 4 cut(s) 444, 867, 893, 976
BmrFI CCNGG 4 cut(s) 1041, 1065, 1070, 1199
BmsI GCATC 5 cut(s) 136, 487, 643, 891, 1249
BpmI CTGGAG 1 cut(s) 617
Bpu10I CCTNAGC 1 cut(s) 1227
Bpu1102I GCTNAGC 1 cut(s) 622
BpuMI CCSGG 1 cut(s) 1041
BsaAI YACGTR 2 cut(s) 170, 856
BsaJI CCNNGG 6 cut(s) 401, 446, 895, 1039, 1063, 1219
BsaWI WCCGGW 1 cut(s) 25
BsaXI ACNNNNNCTCC 4 cut(s) 1035, 1065, 1179, 1209
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 18, 402, 1046, 1069
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 5 cut(s) 125, 312, 557, 1094, 1194
Bse3DI GCAATG 2 cut(s) 697, 1269
BseBI CCWGG 3 cut(s) 1065, 1070, 1199
BseDI CCNNGG 6 cut(s) 401, 446, 895, 1039, 1063, 1219
BseGI GGATG 5 cut(s) 37, 415, 586, 880, 906
BseLI CCNNNNNNNGG 5 cut(s) 17, 18, 402, 1046, 1069
BseMI GCAATG 2 cut(s) 697, 1269
BseMII CTCAG 3 cut(s) 1138, 1165, 1218
BseNI ACTGG 5 cut(s) 125, 312, 557, 1094, 1194
BseXI GCAGC 3 cut(s) 467, 512, 630
Bsh1285I CGRYCG 1 cut(s) 1177
BshFI GGCC 5 cut(s) 124, 406, 685, 1032, 1068
BshNI GGYRCC 2 cut(s) 891, 974
BshTI ACCGGT 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 1177
BsiHKCI CYCGRG 1 cut(s) 1112
BsiSI CCGG 2 cut(s) 26, 1041
BslFI GGGAC 4 cut(s) 209, 298, 595, 879
BslI CCNNNNNNNGG 5 cut(s) 17, 18, 402, 1046, 1069
BsmAI GTCTC 2 cut(s) 490, 552
BsmFI GGGAC 4 cut(s) 209, 298, 595, 879
BsmI GAATGC 1 cut(s) 1119
BsnI GGCC 5 cut(s) 124, 406, 685, 1032, 1068
BsoBI CYCGRG 1 cut(s) 1112
Bsp1286I GDGCHC 1 cut(s) 12
Bsp1407I TGTACA 2 cut(s) 110, 761
Bsp143I GATC 8 cut(s) 262, 310, 412, 442, 479, 633, 1243, 1275
Bsp1720I GCTNAGC 1 cut(s) 622
BspACI CCGC 2 cut(s) 616, 1267
BspANI GGCC 5 cut(s) 124, 406, 685, 1032, 1068
BspCNI CTCAG 3 cut(s) 1137, 1164, 1219
BspLI GGNNCC 4 cut(s) 444, 867, 893, 976
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 4 cut(s) 318, 437, 450, 628
BspT107I GGYRCC 2 cut(s) 891, 974
BsrDI GCAATG 2 cut(s) 697, 1269
BsrFI RCCGGY 1 cut(s) 25
BsrGI TGTACA 2 cut(s) 110, 761
BsrI ACTGG 5 cut(s) 125, 312, 557, 1094, 1194
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 6 cut(s) 401, 446, 895, 1039, 1063, 1219
BssMI GATC 8 cut(s) 262, 310, 412, 442, 479, 633, 1243, 1275
BssNAI GTATAC 1 cut(s) 393
BssSI CACGAG 2 cut(s) 657, 1294
BssT1I CCWWGG 2 cut(s) 401, 895
Bst1107I GTATAC 1 cut(s) 393
Bst2BI CACGAG 2 cut(s) 657, 1294
Bst2UI CCWGG 3 cut(s) 1065, 1070, 1199
Bst4CI ACNGT 7 cut(s) 190, 253, 450, 680, 870, 920, 1175
Bst6I CTCTTC 2 cut(s) 70, 1168
BstAPI GCANNNNNTGC 2 cut(s) 461, 701
BstAUI TGTACA 2 cut(s) 110, 761
BstBAI YACGTR 2 cut(s) 170, 856
BstC8I GCNNGC 3 cut(s) 408, 628, 1133
BstDEI CTNAG 4 cut(s) 622, 1124, 1151, 1227
BstDSI CCRYGG 1 cut(s) 446
BstF5I GGATG 5 cut(s) 37, 415, 586, 880, 906
BstHHI GCGC 4 cut(s) 649, 741, 1010, 1119
BstKTI GATC 8 cut(s) 265, 313, 415, 445, 482, 636, 1246, 1278
BstMAI GTCTC 2 cut(s) 490, 552
BstMBI GATC 8 cut(s) 262, 310, 412, 442, 479, 633, 1243, 1275
BstMCI CGRYCG 1 cut(s) 1177
BstMWI GCNNNNNNNGC 6 cut(s) 461, 627, 653, 701, 910, 1237
BstNI CCWGG 3 cut(s) 1065, 1070, 1199
BstNSI RCATGY 2 cut(s) 517, 1290
BstSCI CCNGG 4 cut(s) 1039, 1063, 1068, 1197
BstV1I GCAGC 3 cut(s) 467, 512, 630
BstX2I RGATCY 3 cut(s) 442, 633, 1275
BstYI RGATCY 3 cut(s) 442, 633, 1275
BstZ17I GTATAC 1 cut(s) 393
BsuRI GGCC 5 cut(s) 124, 406, 685, 1032, 1068
BtgI CCRYGG 1 cut(s) 446
BtsCI GGATG 5 cut(s) 37, 415, 586, 880, 906
BtsIMutI CAGTG 4 cut(s) 305, 550, 587, 1132
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 3 cut(s) 408, 628, 1133
CfoI GCGC 4 cut(s) 649, 741, 1010, 1119
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 4 cut(s) 122, 683, 866, 1223
Csp6I GTAC 5 cut(s) 111, 762, 909, 1147, 1216
CspAI ACCGGT 1 cut(s) 25
CviAII CATG 4 cut(s) 281, 514, 638, 1287
CviQI GTAC 5 cut(s) 111, 762, 909, 1147, 1216
DdeI CTNAG 4 cut(s) 622, 1124, 1151, 1227
DpnI GATC 8 cut(s) 264, 312, 414, 444, 481, 635, 1245, 1277
DpnII GATC 8 cut(s) 262, 310, 412, 442, 479, 633, 1243, 1275
EaeI YGGCCR 2 cut(s) 404, 1066
Eam1104I CTCTTC 2 cut(s) 70, 1168
EarI CTCTTC 2 cut(s) 70, 1168
Eco130I CCWWGG 2 cut(s) 401, 895
Eco24I GRGCYC 1 cut(s) 12
Eco47I GGWCC 2 cut(s) 866, 1223
Eco72I CACGTG 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 1112
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 3 cut(s) 1063, 1068, 1197
EcoT14I CCWWGG 2 cut(s) 401, 895
EcoT22I ATGCAT 1 cut(s) 1288
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 2 cut(s) 401, 895
FaeI CATG 4 cut(s) 284, 517, 641, 1290
FaqI GGGAC 4 cut(s) 209, 298, 595, 879
FatI CATG 4 cut(s) 280, 513, 637, 1286
FauI CCCGC 1 cut(s) 1260
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 392
Fnu4HI GCNGC 3 cut(s) 456, 501, 619
FokI GGATG 5 cut(s) 44, 402, 593, 867, 913
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 3 cut(s) 456, 501, 619
FspBI CTAG 1 cut(s) 687
GlaI GCGC 4 cut(s) 648, 740, 1009, 1118
GluI GCNGC 3 cut(s) 456, 501, 619
GsuI CTGGAG 1 cut(s) 617
HaeIII GGCC 5 cut(s) 124, 406, 685, 1032, 1068
HapII CCGG 2 cut(s) 26, 1041
HhaI GCGC 4 cut(s) 649, 741, 1010, 1119
Hin1II CATG 4 cut(s) 284, 517, 641, 1290
Hin6I GCGC 4 cut(s) 647, 739, 1008, 1117
HinP1I GCGC 4 cut(s) 647, 739, 1008, 1117
HincII GTYRAC 1 cut(s) 1091
HindII GTYRAC 1 cut(s) 1091
HinfI GANTC 6 cut(s) 592, 608, 784, 1020, 1110, 1155
HpaII CCGG 2 cut(s) 26, 1041
HphI GGTGA 4 cut(s) 61, 380, 506, 1283
Hpy166II GTNNAC 3 cut(s) 393, 849, 1091
Hpy188I TCNGA 8 cut(s) 103, 247, 427, 442, 817, 874, 1109, 1154
Hpy188III TCNNGA 7 cut(s) 385, 596, 788, 1167, 1178, 1226, 1296
Hpy8I GTNNAC 3 cut(s) 393, 849, 1091
HpyAV CCTTC 6 cut(s) 38, 499, 941, 985, 1043, 1178
HpyCH4III ACNGT 7 cut(s) 190, 253, 450, 680, 870, 920, 1175
HpyCH4IV ACGT 3 cut(s) 169, 389, 855
HpyCH4V TGCA 8 cut(s) 212, 360, 500, 517, 695, 1135, 1262, 1286
HpyF10VI GCNNNNNNNGC 6 cut(s) 461, 627, 653, 701, 910, 1237
HpyF3I CTNAG 4 cut(s) 622, 1124, 1151, 1227
HpySE526I ACGT 3 cut(s) 169, 389, 855
Hsp92II CATG 4 cut(s) 284, 517, 641, 1290
HspAI GCGC 4 cut(s) 647, 739, 1008, 1117
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 8 cut(s) 262, 310, 412, 442, 479, 633, 1243, 1275
LmnI GCTCC 1 cut(s) 598
Lsp1109I GCAGC 3 cut(s) 467, 512, 630
LweI GCATC 5 cut(s) 136, 487, 643, 891, 1249
MaeI CTAG 1 cut(s) 687
MaeII ACGT 3 cut(s) 169, 389, 855
MaeIII GTNAC 1 cut(s) 1232
MalI GATC 8 cut(s) 264, 312, 414, 444, 481, 635, 1245, 1277
MboI GATC 8 cut(s) 262, 310, 412, 442, 479, 633, 1243, 1275
MboII GAAGA 9 cut(s) 74, 87, 155, 187, 197, 272, 323, 568, 1155
MfeI CAATTG 2 cut(s) 886, 979
MflI RGATCY 3 cut(s) 442, 633, 1275
MhlI GDGCHC 1 cut(s) 12
MlsI TGGCCA 2 cut(s) 406, 1068
MluCI AATT 7 cut(s) 104, 255, 525, 731, 753, 886, 979
MluNI TGGCCA 2 cut(s) 406, 1068
MlyI GAGTC 3 cut(s) 617, 1104, 1149
Mox20I TGGCCA 2 cut(s) 406, 1068
Mph1103I ATGCAT 1 cut(s) 1288
MscI TGGCCA 2 cut(s) 406, 1068
MseI TTAA 4 cut(s) 116, 180, 321, 665
MslI CAYNNNNRTG 1 cut(s) 642
Msp20I TGGCCA 2 cut(s) 406, 1068
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 2 cut(s) 26, 1041
MspR9I CCNGG 4 cut(s) 1041, 1065, 1070, 1199
MunI CAATTG 2 cut(s) 886, 979
Mva1269I GAATGC 1 cut(s) 1119
MvaI CCWGG 3 cut(s) 1065, 1070, 1199
MwoI GCNNNNNNNGC 6 cut(s) 461, 627, 653, 701, 910, 1237
NciI CCSGG 1 cut(s) 1041
NdeII GATC 8 cut(s) 262, 310, 412, 442, 479, 633, 1243, 1275
NlaIII CATG 4 cut(s) 284, 517, 641, 1290
NlaIV GGNNCC 4 cut(s) 444, 867, 893, 976
NsiI ATGCAT 1 cut(s) 1288
NspI RCATGY 2 cut(s) 517, 1290
PaeR7I CTCGAG 1 cut(s) 1112
PctI GAATGC 1 cut(s) 1119
PfeI GAWTC 3 cut(s) 592, 784, 1020
PflFI GACNNNGTC 1 cut(s) 544
PfoI TCCNGGA 1 cut(s) 1197
PinAI ACCGGT 1 cut(s) 25
PkrI GCNGC 3 cut(s) 457, 502, 620
PleI GAGTC 3 cut(s) 616, 1104, 1149
PmaCI CACGTG 1 cut(s) 170
PmlI CACGTG 1 cut(s) 170
PpsI GAGTC 3 cut(s) 616, 1104, 1149
Ppu21I YACGTR 2 cut(s) 170, 856
PshBI ATTAAT 1 cut(s) 180
Psp6I CCWGG 3 cut(s) 1063, 1068, 1197
PspCI CACGTG 1 cut(s) 170
PspGI CCWGG 3 cut(s) 1063, 1068, 1197
PspN4I GGNNCC 4 cut(s) 444, 867, 893, 976
PspPI GGNCC 4 cut(s) 122, 683, 866, 1223
PspXI VCTCGAGB 1 cut(s) 1112
PsuI RGATCY 3 cut(s) 442, 633, 1275
PsyI GACNNNGTC 1 cut(s) 544
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 5 cut(s) 112, 763, 910, 1148, 1217
RsaNI GTAC 5 cut(s) 111, 762, 909, 1147, 1216
RseI CAYNNNNRTG 1 cut(s) 642
SaqAI TTAA 4 cut(s) 116, 180, 321, 665
SatI GCNGC 3 cut(s) 456, 501, 619
Sau3AI GATC 8 cut(s) 262, 310, 412, 442, 479, 633, 1243, 1275
Sau96I GGNCC 4 cut(s) 122, 683, 866, 1223
SchI GAGTC 3 cut(s) 617, 1104, 1149
ScrFI CCNGG 4 cut(s) 1041, 1065, 1070, 1199
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 5 cut(s) 136, 487, 643, 891, 1249
Sfr274I CTCGAG 1 cut(s) 1112
SinI GGWCC 2 cut(s) 866, 1223
SlaI CTCGAG 1 cut(s) 1112
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 1 cut(s) 1112
SmoI CTYRAG 1 cut(s) 1112
Sse9I AATT 7 cut(s) 104, 255, 525, 731, 753, 886, 979
SsiI CCGC 2 cut(s) 616, 1267
SspMI CTAG 1 cut(s) 687
StyD4I CCNGG 4 cut(s) 1039, 1063, 1068, 1197
StyI CCWWGG 2 cut(s) 401, 895
TaaI ACNGT 7 cut(s) 190, 253, 450, 680, 870, 920, 1175
TaiI ACGT 3 cut(s) 172, 392, 858
TaqI TCGA 5 cut(s) 155, 478, 787, 1113, 1177
TaqII GACCGA 2 cut(s) 1093, 1211
TasI AATT 7 cut(s) 104, 255, 525, 731, 753, 886, 979
TatI WGTACW 2 cut(s) 110, 761
TfiI GAWTC 3 cut(s) 592, 784, 1020
Tru1I TTAA 4 cut(s) 116, 180, 321, 665
Tru9I TTAA 4 cut(s) 116, 180, 321, 665
TscAI CASTG 4 cut(s) 312, 557, 594, 1132
TseI GCWGC 3 cut(s) 455, 500, 618
TspDTI ATGAA 4 cut(s) 48, 190, 444, 1197
TspRI CASTG 4 cut(s) 312, 557, 594, 1132
Tth111I GACNNNGTC 1 cut(s) 544
VpaK11BI GGWCC 2 cut(s) 866, 1223
VspI ATTAAT 1 cut(s) 180
XapI RAATTY 3 cut(s) 104, 731, 753
XceI RCATGY 2 cut(s) 517, 1290
XhoI CTCGAG 1 cut(s) 1112
XmiI GTMKAC 1 cut(s) 392
XspI CTAG 1 cut(s) 687
Zsp2I ATGCAT 1 cut(s) 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.