pycom08g03480

K( ) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005607
Physical Location & Seq
Forward (+)
2735333 .. 2739638
4306 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03480.16

Sequence Viewer

Length: 1095 bp
ATGATTGACAAAGCTCTGGAGAACGAGTTCAAGGAGAATGACCAGAACGAAGCTACTGATCCTGGAAGTTTCAACAACAGCGTCGCCGGCCAGCAGGCGGTACTGGAAACAGTTGCGAGAGTAAAGTCGAAGAAAAATGAGACGAAAGAAGAAAAATCGTTTCAACTACATGATGTTTTCAATCTGGATAATGATAATAGAGGAGAAGATACACCAACAATGATCGATCGCAAGGACAATGTCTTCATAATATCGAATTTTAAATCAAAATATCCAGTGCTGCAACTAGACTTGAGATTAATATCGGATTTGGTGGTTGTTATTGTATCTGCAACCTGTGGTGGCATTGCCTTTGCTTGCGCTGGACAACCGGTTATTAGTGGATATTTGCTAGATCGATCCGTTATTGGACCTGGAGGTTTCAACTTCGTCAGTGAAATGGTTCAAGTTGAAACTGTGGCTCAGTTTGGGGTAATCTTTCTTCTTTTTGCTCTGGGTTTGGAGTTCTCCACAGCAAAGCTTCGAGTTGTTCGAGCGGTTGCTGTTCCAGGAGGGCTGCTACAAATCTTCCTATTTATGTGCCTGTGTGGTATTACCGCCTCATTATGTGGTGGTAAAGTTTCTGAGGGGATATTCGTCGGTGTGTTCCTTTCTATGTCTTCAACAGCAGTGGTGTTGAAATTTTTGATGGAAAAGAACTCCACTAATGCCCTTCATGGCCAAGTCACCATTGGCACCCTTATTCTGCAGGATTGTGCTGTTGGTTTGCTGTTTGCTTTGCTTCCAGTTTTGGGTGGGACTTCGGGTATTCTTCAAGGAGTGATGTCTATGGCTAAGCTGATGGTGACGTTGATTACATTTTTGGCTGTTCTTTCTATTTCATCTCGTACTTGTGTTCCTTGGTTACTTAAACTGATGATAAGTTTATCTTCACAGACCAATGAACTCTACCAATTGGCATCAGTTGCTTTCTGCTTGCTTGTTGCCTGGTGTAGTGATAAGCTGGGACTAAGTCTGGAACTGGGTTCCTTCGCTGCGGGAGTGATGATATCAACAACTTTTCGATCTTGCACAACATACACTAGAACAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

365

Amino Acids

39.18

Weight (kDa)

5.56

Isoelectric Point (pI)

27.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 734
AccBSI CCGCTC 1 cut(s) 536
AciI CCGC 4 cut(s) 98, 536, 597, 1037
AclWI GGATC 2 cut(s) 53, 393
AcoI YGGCCR 2 cut(s) 88, 718
AcsI RAATTY 2 cut(s) 256, 680
AfaI GTAC 2 cut(s) 102, 889
AfiI CCNNNNNNNGG 2 cut(s) 97, 791
AgeI ACCGGT 1 cut(s) 370
AjnI CCWGG 4 cut(s) 61, 412, 547, 986
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 5 cut(s) 14, 53, 520, 838, 1003
AluI AGCT 5 cut(s) 14, 53, 520, 838, 1003
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 2 cut(s) 53, 393
AoxI GGCC 2 cut(s) 88, 718
ApeKI GCWGC 3 cut(s) 280, 556, 1034
ApoI RAATTY 2 cut(s) 256, 680
AseI ATTAAT 1 cut(s) 299
AsiGI ACCGGT 1 cut(s) 370
Asp700I GAANNNNTTC 2 cut(s) 26, 441
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 1 cut(s) 410
AsuHPI GGTGA 2 cut(s) 718, 856
AvaII GGWCC 1 cut(s) 410
BalI TGGCCA 1 cut(s) 720
BanI GGYRCC 1 cut(s) 734
BarI GAAGNNNNNNTAC 2 cut(s) 465, 497
BbsI GAAGAC 2 cut(s) 235, 651
BbvI GCAGC 3 cut(s) 267, 543, 1021
BccI CCATC 2 cut(s) 682, 835
BciT130I CCWGG 4 cut(s) 63, 414, 549, 988
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 3 cut(s) 287, 392, 1083
BfmI CTRYAG 1 cut(s) 746
BisI GCNGC 3 cut(s) 281, 557, 1035
BlpI GCTNAGC 1 cut(s) 834
BlsI GCNGC 3 cut(s) 282, 558, 1036
Bme1390I CCNGG 4 cut(s) 63, 414, 549, 988
Bme18I GGWCC 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 2 cut(s) 736, 1027
BmrFI CCNGG 4 cut(s) 63, 414, 549, 988
BmrI ACTGGG 1 cut(s) 1031
BmsI GCATC 1 cut(s) 968
BmuI ACTGGG 1 cut(s) 1031
BpiI GAAGAC 2 cut(s) 235, 651
BpmI CTGGAG 2 cut(s) 38, 435
Bpu1102I GCTNAGC 1 cut(s) 834
BpuEI CTTGAG 1 cut(s) 313
Bsa29I ATCGAT 2 cut(s) 225, 397
BsaBI GATNNNNATC 1 cut(s) 301
BsaJI CCNNGG 1 cut(s) 899
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 791
Bse118I RCCGGY 2 cut(s) 86, 370
Bse1I ACTGG 4 cut(s) 108, 275, 785, 1026
Bse3DI GCAATG 1 cut(s) 345
Bse8I GATNNNNATC 1 cut(s) 301
BseBI CCWGG 4 cut(s) 63, 414, 549, 988
BseCI ATCGAT 2 cut(s) 225, 397
BseDI CCNNGG 1 cut(s) 899
BseJI GATNNNNATC 1 cut(s) 301
BseLI CCNNNNNNNGG 2 cut(s) 97, 791
BseMI GCAATG 1 cut(s) 345
BseMII CTCAG 2 cut(s) 476, 615
BseNI ACTGG 4 cut(s) 108, 275, 785, 1026
BseRI GAGGAG 1 cut(s) 216
BseXI GCAGC 3 cut(s) 267, 543, 1021
BseYI CCCAGC 1 cut(s) 1003
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 2 cut(s) 90, 720
BshNI GGYRCC 1 cut(s) 734
BshTI ACCGGT 1 cut(s) 370
BshVI ATCGAT 2 cut(s) 225, 397
BsiEI CGRYCG 1 cut(s) 229
BsiSI CCGG 2 cut(s) 87, 371
BslFI GGGAC 2 cut(s) 811, 1020
BslI CCNNNNNNNGG 2 cut(s) 97, 791
BsmAI GTCTC 1 cut(s) 134
BsmBI CGTCTC 1 cut(s) 134
BsmFI GGGAC 2 cut(s) 811, 1020
BsnI GGCC 2 cut(s) 90, 720
Bsp143I GATC 6 cut(s) 58, 222, 226, 394, 398, 1064
Bsp1720I GCTNAGC 1 cut(s) 834
BspACI CCGC 4 cut(s) 98, 536, 597, 1037
BspANI GGCC 2 cut(s) 90, 720
BspCNI CTCAG 2 cut(s) 475, 616
BspDI ATCGAT 2 cut(s) 225, 397
BspLI GGNNCC 2 cut(s) 736, 1027
BspMAI CTGCAG 1 cut(s) 750
BspPI GGATC 2 cut(s) 53, 393
BspT107I GGYRCC 1 cut(s) 734
BsrBI CCGCTC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 345
BsrFI RCCGGY 2 cut(s) 86, 370
BsrI ACTGG 4 cut(s) 108, 275, 785, 1026
BssAI RCCGGY 2 cut(s) 86, 370
BssECI CCNNGG 1 cut(s) 899
BssMI GATC 6 cut(s) 58, 222, 226, 394, 398, 1064
BssT1I CCWWGG 1 cut(s) 899
Bst2UI CCWGG 4 cut(s) 63, 414, 549, 988
Bst4CI ACNGT 2 cut(s) 112, 457
BstAPI GCANNNNNTGC 1 cut(s) 965
BstC8I GCNNGC 5 cut(s) 88, 92, 96, 358, 977
BstDEI CTNAG 4 cut(s) 462, 624, 834, 1010
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 6 cut(s) 61, 225, 229, 397, 401, 1067
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 6 cut(s) 58, 222, 226, 394, 398, 1064
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 2 cut(s) 87, 965
BstNI CCWGG 4 cut(s) 63, 414, 549, 988
BstSCI CCNGG 4 cut(s) 61, 412, 547, 986
BstSFI CTRYAG 1 cut(s) 746
BstV1I GCAGC 3 cut(s) 267, 543, 1021
BstV2I GAAGAC 2 cut(s) 235, 651
Bsu15I ATCGAT 2 cut(s) 225, 397
BsuRI GGCC 2 cut(s) 90, 720
BsuTUI ATCGAT 2 cut(s) 225, 397
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 3 cut(s) 282, 439, 675
Cac8I GCNNGC 5 cut(s) 88, 92, 96, 358, 977
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 2 cut(s) 86, 370
Cfr13I GGNCC 1 cut(s) 410
ClaI ATCGAT 2 cut(s) 225, 397
CseI GACGC 1 cut(s) 70
Csp6I GTAC 2 cut(s) 101, 888
CspAI ACCGGT 1 cut(s) 370
CspCI CAANNNNNGTGG 2 cut(s) 651, 686
CviAII CATG 2 cut(s) 170, 716
CviQI GTAC 2 cut(s) 101, 888
DdeI CTNAG 4 cut(s) 462, 624, 834, 1010
DpnI GATC 6 cut(s) 60, 224, 228, 396, 400, 1066
DpnII GATC 6 cut(s) 58, 222, 226, 394, 398, 1064
DraI TTTAAA 1 cut(s) 262
EaeI YGGCCR 2 cut(s) 88, 718
Eco130I CCWWGG 1 cut(s) 899
Eco32I GATATC 1 cut(s) 1050
Eco47I GGWCC 1 cut(s) 410
EcoRII CCWGG 4 cut(s) 61, 412, 547, 986
EcoRV GATATC 1 cut(s) 1050
EcoT14I CCWWGG 1 cut(s) 899
ErhI CCWWGG 1 cut(s) 899
Esp3I CGTCTC 1 cut(s) 134
FaeI CATG 2 cut(s) 173, 719
FaiI YATR 8 cut(s) 171, 248, 578, 607, 656, 717, 830, 1078
FalI AAGNNNNNCTT 2 cut(s) 913, 945
FaqI GGGAC 2 cut(s) 811, 1020
FatI CATG 2 cut(s) 169, 715
FauI CCCGC 1 cut(s) 1030
Fnu4HI GCNGC 3 cut(s) 281, 557, 1035
Fsp4HI GCNGC 3 cut(s) 281, 557, 1035
FspBI CTAG 3 cut(s) 287, 392, 1083
GlaI GCGC 1 cut(s) 361
GluI GCNGC 3 cut(s) 281, 557, 1035
GsaI CCCAGC 1 cut(s) 1007
GsuI CTGGAG 2 cut(s) 38, 435
HaeIII GGCC 2 cut(s) 90, 720
HapII CCGG 2 cut(s) 87, 371
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 362
Hin1II CATG 2 cut(s) 173, 719
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HindIII AAGCTT 1 cut(s) 518
HpaII CCGG 2 cut(s) 87, 371
HphI GGTGA 2 cut(s) 718, 856
Hpy188I TCNGA 2 cut(s) 307, 625
Hpy188III TCNNGA 3 cut(s) 17, 185, 1016
Hpy99I CGWCG 2 cut(s) 86, 641
HpyAV CCTTC 2 cut(s) 722, 1039
HpyCH4III ACNGT 2 cut(s) 112, 457
HpyCH4IV ACGT 1 cut(s) 848
HpyCH4V TGCA 4 cut(s) 283, 332, 748, 1071
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 965
HpyF3I CTNAG 4 cut(s) 462, 624, 834, 1010
HpySE526I ACGT 1 cut(s) 848
Hsp92II CATG 2 cut(s) 173, 719
HspAI GCGC 1 cut(s) 360
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 6 cut(s) 58, 222, 226, 394, 398, 1064
Lsp1109I GCAGC 3 cut(s) 267, 543, 1021
LweI GCATC 1 cut(s) 968
MaeI CTAG 3 cut(s) 287, 392, 1083
MaeII ACGT 1 cut(s) 848
MaeIII GTNAC 3 cut(s) 724, 844, 903
MalI GATC 6 cut(s) 60, 224, 228, 396, 400, 1066
MbiI CCGCTC 1 cut(s) 536
MboI GATC 6 cut(s) 58, 222, 226, 394, 398, 1064
MboII GAAGA 9 cut(s) 142, 161, 218, 235, 473, 559, 651, 803, 921
MfeI CAATTG 1 cut(s) 953
MlsI TGGCCA 1 cut(s) 720
MluCI AATT 4 cut(s) 256, 680, 953, 1090
MluNI TGGCCA 1 cut(s) 720
MnlI CCTC 5 cut(s) 194, 410, 545, 610, 619
Mox20I TGGCCA 1 cut(s) 720
MroNI GCCGGC 1 cut(s) 86
MroXI GAANNNNTTC 2 cut(s) 26, 441
MscI TGGCCA 1 cut(s) 720
MseI TTAA 3 cut(s) 261, 299, 909
Msp20I TGGCCA 1 cut(s) 720
MspI CCGG 2 cut(s) 87, 371
MspR9I CCNGG 4 cut(s) 63, 414, 549, 988
MunI CAATTG 1 cut(s) 953
MvaI CCWGG 4 cut(s) 63, 414, 549, 988
MwoI GCNNNNNNNGC 2 cut(s) 87, 965
NaeI GCCGGC 1 cut(s) 88
NdeII GATC 6 cut(s) 58, 222, 226, 394, 398, 1064
NgoMIV GCCGGC 1 cut(s) 86
NlaIII CATG 2 cut(s) 173, 719
NlaIV GGNNCC 2 cut(s) 736, 1027
NmuCI GTSAC 2 cut(s) 724, 844
PcsI WCGNNNNNNNCGW 1 cut(s) 529
PdiI GCCGGC 1 cut(s) 88
PdmI GAANNNNTTC 2 cut(s) 26, 441
PflFI GACNNNGTC 2 cut(s) 239, 1011
PfoI TCCNGGA 2 cut(s) 61, 547
PinAI ACCGGT 1 cut(s) 370
PkrI GCNGC 3 cut(s) 282, 558, 1036
Ple19I CGATCG 1 cut(s) 229
PshBI ATTAAT 1 cut(s) 299
Psp6I CCWGG 4 cut(s) 61, 412, 547, 986
PspFI CCCAGC 1 cut(s) 1003
PspGI CCWGG 4 cut(s) 61, 412, 547, 986
PspN4I GGNNCC 2 cut(s) 736, 1027
PspPI GGNCC 1 cut(s) 410
PstI CTGCAG 1 cut(s) 750
PsyI GACNNNGTC 2 cut(s) 239, 1011
PvuI CGATCG 1 cut(s) 229
RsaI GTAC 2 cut(s) 102, 889
RsaNI GTAC 2 cut(s) 101, 888
SaqAI TTAA 3 cut(s) 261, 299, 909
SatI GCNGC 3 cut(s) 281, 557, 1035
Sau3AI GATC 6 cut(s) 58, 222, 226, 394, 398, 1064
Sau96I GGNCC 1 cut(s) 410
ScrFI CCNGG 4 cut(s) 63, 414, 549, 988
SetI ASST 9 cut(s) 16, 55, 338, 415, 421, 522, 840, 851, 1005
SfaNI GCATC 1 cut(s) 968
SfcI CTRYAG 1 cut(s) 746
SinI GGWCC 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
Sse9I AATT 4 cut(s) 256, 680, 953, 1090
SsiI CCGC 4 cut(s) 98, 536, 597, 1037
SspMI CTAG 3 cut(s) 287, 392, 1083
StyD4I CCNGG 4 cut(s) 61, 412, 547, 986
StyI CCWWGG 1 cut(s) 899
TaaI ACNGT 2 cut(s) 112, 457
TaiI ACGT 1 cut(s) 851
TaqI TCGA 7 cut(s) 128, 225, 254, 397, 523, 532, 1063
TasI AATT 4 cut(s) 256, 680, 953, 1090
Tru1I TTAA 3 cut(s) 261, 299, 909
Tru9I TTAA 3 cut(s) 261, 299, 909
TscAI CASTG 3 cut(s) 282, 439, 675
TseFI GTSAC 2 cut(s) 724, 844
TseI GCWGC 3 cut(s) 280, 556, 1034
Tsp45I GTSAC 2 cut(s) 724, 844
TspDTI ATGAA 4 cut(s) 235, 704, 870, 957
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 3 cut(s) 282, 439, 675
Tth111I GACNNNGTC 2 cut(s) 239, 1011
VpaK11BI GGWCC 1 cut(s) 410
VspI ATTAAT 1 cut(s) 299
XapI RAATTY 2 cut(s) 256, 680
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmnI GAANNNNTTC 2 cut(s) 26, 441
XspI CTAG 3 cut(s) 287, 392, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.