Rorug06G0382600

K( ) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005607
Physical Location & Seq
Reverse (-)
52840458 .. 52841762
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0382600.1

Sequence Viewer

Length: 1305 bp
ATGAGCGCCGAGAAGCTTCCGTTCATGCTCCCTGCCGTCTTCACAATCGGCCCCCACATTGACGACATGGATTCCGTCAAGAAGTACGCCAAGCTGCTCTCGAACCATGACAAGAACTCCAACCACGTGAAGGAGCTGGTCCAGGGCGTCATCGAGGGCGAGACTCGTGTGCTCGCGGCCTCTATGACGATGGAGGAGGTGTTCAAGGGCACCAAAGAGTTTAAGAAGGAGGTTTGGGACAAGGTTCAGCTGGAGCTCAACCAGTTCGGGCTTATAATTTACAATGCGAATGTCAAACAGTTGGTGGACGTTCAAGGACATGAGTATTTCAGTTACTTGGGCCAGAAGACTCAGATGGAGGCGGCCAACCAGGCCAAGGTTGACGTGGCGGAGGCCAGGATGAAGGGGGAGGTTGGCGCCAAGTTGAGGGAGGGGCAGACCCTGCAGAATGCGGCGAAGATCAATGCAGAGACGAAGATCATATCGACTCAGAGGGAAGGAGAGGGGAAGAAGGAGGAGATTAGGGTGAAGACGGAGGTCAAGGTTTATGAGAATCAGAAGGAGGCTGAGGTGGCTCAGGCCAATGCTGAGCTGGCAATGAAGAAGGCTGGATGGGCCAAGGAGGCGCAGGTGGCGCAGGTGGAGTCGACCAAGGCGGTGGCGTTGAGGGAAGCCGAGTTGCAGAAGAAGGTGGAGACGATGAATGCCCAGACTACGACCGAAAAGCTCAAGGCTGAGCTCTTGAGCAAGGCCAGCGTTGAGGCTGAAACGAAGGCGCGGGAGGCAGAATGGAATCTCTACCAGAAGCAGAAGGCAGCGGATGCCATTCTTTACCAAAAGAAGAAAGAGGCTGAGGCTACGGAAACAGCTGCTCAAGCACAGTTGTTTGCTCGTCAAAAGGCTGCTGATGGAGAATTCTATGCAAAGAAGAAAGAGGCCGAGGGACTGGTGGCACTCGGACAAGCCCACGGCGCTTATCTGCACACTCTTCTGGAGGCAAATGGAGGCGACTATGCAGCTGCGAGAGACTACATGATGATCAATGGTGGGATGTATTCGGCGATTGCAAGGATTAATGCTGACGCCGTGAAAGGACTTCAGCCCAAGATTAGCATCTGGACTAATGGGGGAGACGCTGCAGACGGTGGTGCTGGTGCTGGCAATGGCAATGCAGCTATGAAGGAGATTGCCGGCCTTTATAAGACACTGCCACCATTGTTACAGACGGTTCAAGACCAAACTGGAATGCAGCCTCCCAGTTGGATGGGAACTTTGTCAGTTGAAGATTCTCAGCGTAGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

434

Amino Acids

47.56

Weight (kDa)

6.4

Isoelectric Point (pI)

23.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 2 - 132 3.3e-15 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 275, 1200
AarI CACCTGC 2 cut(s) 619, 628
Acc36I ACCTGC 2 cut(s) 619, 628
AccB1I GGYRCC 2 cut(s) 209, 416
AccI GTMKAC 1 cut(s) 647
AccII CGCG 2 cut(s) 176, 778
AciI CCGC 7 cut(s) 176, 362, 389, 452, 656, 778, 818
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 1 cut(s) 914
AcuI CTGAAG 1 cut(s) 1082
AcvI CACGTG 1 cut(s) 127
AcyI GRCGYC 3 cut(s) 147, 417, 1083
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 3 cut(s) 130, 376, 426
AgsI TTSAA 4 cut(s) 205, 314, 1232, 1283
AjiI CACGTC 1 cut(s) 385
AjnI CCWGG 3 cut(s) 141, 369, 395
AloI GAACNNNNNNTCC 2 cut(s) 185, 217
Alw21I GWGCWC 3 cut(s) 174, 258, 741
Alw26I GTCTC 5 cut(s) 155, 464, 689, 1020, 1125
AlwNI CAGNNNCTG 1 cut(s) 442
ApeKI GCWGC 9 cut(s) 94, 815, 869, 902, 1016, 1019, 1136, 1172, 1249
ApoI RAATTY 1 cut(s) 914
AseI ATTAAT 1 cut(s) 1074
AspLEI GCGC 6 cut(s) 8, 419, 628, 637, 778, 974
AspS9I GGNCC 4 cut(s) 50, 139, 340, 615
AsuHPI GGTGA 1 cut(s) 538
AvaII GGWCC 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 212
BanI GGYRCC 2 cut(s) 209, 416
BanII GRGCYC 2 cut(s) 258, 741
BauI CACGAG 1 cut(s) 165
BbrPI CACGTG 1 cut(s) 127
BbsI GAAGAC 3 cut(s) 31, 353, 536
Bbv12I GWGCWC 3 cut(s) 174, 258, 741
BbvCI CCTCAGC 2 cut(s) 567, 852
BbvI GCAGC 9 cut(s) 81, 827, 856, 889, 1006, 1028, 1123, 1184, 1261
BccI CCATC 5 cut(s) 184, 349, 606, 902, 1258
BceAI ACGGC 3 cut(s) 20, 985, 1070
BciT130I CCWGG 3 cut(s) 143, 371, 397
BclI TGATCA 1 cut(s) 1038
BcoDI GTCTC 5 cut(s) 155, 464, 689, 1020, 1125
BfmI CTRYAG 2 cut(s) 443, 1137
BfoI RGCGCY 3 cut(s) 9, 420, 975
BfuAI ACCTGC 2 cut(s) 619, 628
BglI GCCNNNNNGGC 2 cut(s) 371, 623
BlpI GCTNAGC 2 cut(s) 588, 735
Bme1390I CCNGG 3 cut(s) 143, 371, 397
Bme18I GGWCC 1 cut(s) 139
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 4 cut(s) 50, 139, 340, 615
BmiI GGNNCC 3 cut(s) 52, 211, 418
BmrFI CCNGG 3 cut(s) 143, 371, 397
BmrI ACTGGG 1 cut(s) 1251
BmsI GCATC 2 cut(s) 811, 1122
BmuI ACTGGG 1 cut(s) 1251
BoxI GACNNNNGTC 1 cut(s) 536
BpiI GAAGAC 3 cut(s) 31, 353, 536
BpmI CTGGAG 2 cut(s) 272, 1013
Bpu10I CCTNAGC 3 cut(s) 567, 576, 852
Bpu1102I GCTNAGC 2 cut(s) 588, 735
BpuEI CTTGAG 3 cut(s) 713, 763, 858
BsaAI YACGTR 1 cut(s) 127
BsaBI GATNNNNATC 1 cut(s) 1112
BsaHI GRCGYC 3 cut(s) 147, 417, 1083
BsaJI CCNNGG 6 cut(s) 142, 375, 618, 651, 939, 967
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 185, 215
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 376, 426
Bse118I RCCGGY 1 cut(s) 1190
Bse1I ACTGG 4 cut(s) 262, 951, 1246, 1257
Bse3DI GCAATG 3 cut(s) 603, 1168, 1174
Bse8I GATNNNNATC 1 cut(s) 1112
BseBI CCWGG 3 cut(s) 143, 371, 397
BseDI CCNNGG 6 cut(s) 142, 375, 618, 651, 939, 967
BseGI GGATG 5 cut(s) 405, 617, 826, 1056, 1269
BseJI GATNNNNATC 1 cut(s) 1112
BseLI CCNNNNNNNGG 3 cut(s) 130, 376, 426
BseMI GCAATG 3 cut(s) 603, 1168, 1174
BseMII CTCAG 8 cut(s) 365, 503, 558, 579, 590, 726, 843, 1304
BseNI ACTGG 4 cut(s) 262, 951, 1246, 1257
BseRI GAGGAG 2 cut(s) 209, 530
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 9 cut(s) 81, 827, 856, 889, 1006, 1028, 1123, 1184, 1261
BsgI GTGCAG 1 cut(s) 965
Bsh1236I CGCG 2 cut(s) 176, 778
Bsh1285I CGRYCG 1 cut(s) 720
BshNI GGYRCC 2 cut(s) 209, 416
BsiEI CGRYCG 1 cut(s) 720
BsiHKAI GWGCWC 3 cut(s) 174, 258, 741
BsiSI CCGG 1 cut(s) 1191
BslFI GGGAC 2 cut(s) 251, 957
BslI CCNNNNNNNGG 3 cut(s) 130, 376, 426
BsmAI GTCTC 5 cut(s) 155, 464, 689, 1020, 1125
BsmBI CGTCTC 3 cut(s) 464, 689, 1125
BsmFI GGGAC 2 cut(s) 251, 957
BsmI GAATGC 3 cut(s) 454, 709, 1251
Bsp1286I GDGCHC 4 cut(s) 174, 212, 258, 741
Bsp143I GATC 3 cut(s) 459, 477, 1038
Bsp1720I GCTNAGC 2 cut(s) 588, 735
BspACI CCGC 7 cut(s) 176, 362, 389, 452, 656, 778, 818
BspCNI CTCAG 8 cut(s) 364, 502, 559, 580, 589, 727, 844, 1303
BspFNI CGCG 2 cut(s) 176, 778
BspLI GGNNCC 3 cut(s) 52, 211, 418
BspMAI CTGCAG 2 cut(s) 447, 1141
BspMI ACCTGC 2 cut(s) 619, 628
BspT107I GGYRCC 2 cut(s) 209, 416
BsrDI GCAATG 3 cut(s) 603, 1168, 1174
BsrFI RCCGGY 1 cut(s) 1190
BsrI ACTGG 4 cut(s) 262, 951, 1246, 1257
BssAI RCCGGY 1 cut(s) 1190
BssECI CCNNGG 6 cut(s) 142, 375, 618, 651, 939, 967
BssMI GATC 3 cut(s) 459, 477, 1038
BssNI GRCGYC 3 cut(s) 147, 417, 1083
BssSI CACGAG 1 cut(s) 165
BssT1I CCWWGG 3 cut(s) 375, 618, 651
Bst2BI CACGAG 1 cut(s) 165
Bst2UI CCWGG 3 cut(s) 143, 371, 397
Bst4CI ACNGT 4 cut(s) 300, 882, 1145, 1228
Bst6I CTCTTC 1 cut(s) 993
BstACI GRCGYC 3 cut(s) 147, 417, 1083
BstAPI GCANNNNNTGC 2 cut(s) 442, 821
BstBAI YACGTR 1 cut(s) 127
BstC8I GCNNGC 5 cut(s) 174, 594, 754, 1159, 1192
BstDEI CTNAG 8 cut(s) 351, 489, 567, 576, 588, 735, 852, 1290
BstDSI CCRYGG 1 cut(s) 967
BstF5I GGATG 5 cut(s) 405, 617, 826, 1056, 1269
BstFNI CGCG 2 cut(s) 176, 778
BstH2I RGCGCY 3 cut(s) 9, 420, 975
BstHHI GCGC 6 cut(s) 8, 419, 628, 637, 778, 974
BstKTI GATC 3 cut(s) 462, 480, 1041
BstMAI GTCTC 5 cut(s) 155, 464, 689, 1020, 1125
BstMBI GATC 3 cut(s) 459, 477, 1038
BstMCI CGRYCG 1 cut(s) 720
BstNI CCWGG 3 cut(s) 143, 371, 397
BstPAI GACNNNNGTC 1 cut(s) 536
BstSCI CCNGG 3 cut(s) 141, 369, 395
BstSFI CTRYAG 2 cut(s) 443, 1137
BstSLI GKGCMC 1 cut(s) 212
BstUI CGCG 2 cut(s) 176, 778
BstV1I GCAGC 9 cut(s) 81, 827, 856, 889, 1006, 1028, 1123, 1184, 1261
BstV2I GAAGAC 3 cut(s) 31, 353, 536
BstXI CCANNNNNNTGG 2 cut(s) 658, 1264
BtgI CCRYGG 1 cut(s) 967
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 5 cut(s) 405, 617, 826, 1056, 1269
BtsI GCAGTG 1 cut(s) 1205
BtsIMutI CAGTG 1 cut(s) 1205
BveI ACCTGC 2 cut(s) 619, 628
Cac8I GCNNGC 5 cut(s) 174, 594, 754, 1159, 1192
CaiI CAGNNNCTG 1 cut(s) 442
CfoI GCGC 6 cut(s) 8, 419, 628, 637, 778, 974
Cfr10I RCCGGY 1 cut(s) 1190
Cfr13I GGNCC 4 cut(s) 50, 139, 340, 615
CseI GACGC 3 cut(s) 136, 1091, 1142
Csp6I GTAC 1 cut(s) 85
CviAII CATG 5 cut(s) 25, 67, 107, 320, 1033
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 8 cut(s) 351, 489, 567, 576, 588, 735, 852, 1290
DinI GGCGCC 1 cut(s) 418
DpnI GATC 3 cut(s) 461, 479, 1040
DpnII GATC 3 cut(s) 459, 477, 1038
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 993
EarI CTCTTC 1 cut(s) 993
EciI GGCGGA 1 cut(s) 404
Ecl136II GAGCTC 2 cut(s) 256, 739
Eco130I CCWWGG 3 cut(s) 375, 618, 651
Eco24I GRGCYC 2 cut(s) 258, 741
Eco47I GGWCC 1 cut(s) 139
Eco53kI GAGCTC 2 cut(s) 256, 739
Eco57I CTGAAG 1 cut(s) 1082
Eco72I CACGTG 1 cut(s) 127
EcoICRI GAGCTC 2 cut(s) 256, 739
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 3 cut(s) 141, 369, 395
EcoT14I CCWWGG 3 cut(s) 375, 618, 651
EcoT38I GRGCYC 2 cut(s) 258, 741
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
ErhI CCWWGG 3 cut(s) 375, 618, 651
Esp3I CGTCTC 3 cut(s) 464, 689, 1125
FaeI CATG 5 cut(s) 28, 70, 110, 323, 1036
FaqI GGGAC 2 cut(s) 251, 957
FatI CATG 5 cut(s) 24, 66, 106, 319, 1032
FauI CCCGC 1 cut(s) 771
FbaI TGATCA 1 cut(s) 1038
FblI GTMKAC 1 cut(s) 647
FokI GGATG 5 cut(s) 412, 624, 833, 1063, 1276
FriOI GRGCYC 2 cut(s) 258, 741
GlaI GCGC 6 cut(s) 7, 418, 627, 636, 777, 973
GsuI CTGGAG 2 cut(s) 272, 1013
HaeII RGCGCY 3 cut(s) 9, 420, 975
HapII CCGG 1 cut(s) 1191
HgaI GACGC 3 cut(s) 136, 1091, 1142
HhaI GCGC 6 cut(s) 8, 419, 628, 637, 778, 974
Hin1I GRCGYC 3 cut(s) 147, 417, 1083
Hin1II CATG 5 cut(s) 28, 70, 110, 323, 1036
Hin6I GCGC 6 cut(s) 6, 417, 626, 635, 776, 972
HinP1I GCGC 6 cut(s) 6, 417, 626, 635, 776, 972
HincII GTYRAC 2 cut(s) 382, 648
HindII GTYRAC 2 cut(s) 382, 648
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 8 cut(s) 71, 163, 349, 487, 553, 644, 793, 1286
HpaII CCGG 1 cut(s) 1191
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 3 cut(s) 307, 382, 648
Hpy188I TCNGA 4 cut(s) 354, 492, 558, 959
Hpy188III TCNNGA 6 cut(s) 79, 100, 742, 992, 1117, 1232
Hpy8I GTNNAC 3 cut(s) 307, 382, 648
HpyCH4III ACNGT 4 cut(s) 300, 882, 1145, 1228
HpyCH4IV ACGT 3 cut(s) 126, 309, 384
HpyF3I CTNAG 8 cut(s) 351, 489, 567, 576, 588, 735, 852, 1290
HpySE526I ACGT 3 cut(s) 126, 309, 384
Hsp92I GRCGYC 3 cut(s) 147, 417, 1083
Hsp92II CATG 5 cut(s) 28, 70, 110, 323, 1036
HspAI GCGC 6 cut(s) 6, 417, 626, 635, 776, 972
KasI GGCGCC 1 cut(s) 416
KroI GCCGGC 1 cut(s) 1190
KroNI GCCGGC 1 cut(s) 1192
Ksp22I TGATCA 1 cut(s) 1038
Kzo9I GATC 3 cut(s) 459, 477, 1038
LmnI GCTCC 3 cut(s) 33, 133, 253
Lsp1109I GCAGC 9 cut(s) 81, 827, 856, 889, 1006, 1028, 1123, 1184, 1261
LweI GCATC 2 cut(s) 811, 1122
MaeII ACGT 3 cut(s) 126, 309, 384
MaeIII GTNAC 2 cut(s) 332, 1218
MalI GATC 3 cut(s) 461, 479, 1040
MboI GATC 3 cut(s) 459, 477, 1038
MhlI GDGCHC 4 cut(s) 174, 212, 258, 741
MluCI AATT 3 cut(s) 276, 914, 1300
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 4 cut(s) 157, 343, 481, 653
MmeI TCCRAC 2 cut(s) 144, 1241
MroNI GCCGGC 1 cut(s) 1190
MseI TTAA 2 cut(s) 222, 1074
MspA1I CMGCKG 4 cut(s) 250, 818, 869, 1019
MspI CCGG 1 cut(s) 1191
MspR9I CCNGG 3 cut(s) 143, 371, 397
Mva1269I GAATGC 3 cut(s) 454, 709, 1251
MvaI CCWGG 3 cut(s) 143, 371, 397
MvnI CGCG 2 cut(s) 176, 778
NaeI GCCGGC 1 cut(s) 1192
NarI GGCGCC 1 cut(s) 417
NdeII GATC 3 cut(s) 459, 477, 1038
NgoMIV GCCGGC 1 cut(s) 1190
NlaIII CATG 5 cut(s) 28, 70, 110, 323, 1036
NlaIV GGNNCC 3 cut(s) 52, 211, 418
NmeAIII GCCGAG 3 cut(s) 34, 700, 964
PaqCI CACCTGC 2 cut(s) 619, 628
PctI GAATGC 3 cut(s) 454, 709, 1251
PdiI GCCGGC 1 cut(s) 1192
PfeI GAWTC 4 cut(s) 71, 553, 793, 1286
PleI GAGTC 4 cut(s) 157, 343, 481, 652
PluTI GGCGCC 1 cut(s) 420
PmaCI CACGTG 1 cut(s) 127
PmlI CACGTG 1 cut(s) 127
PpsI GAGTC 4 cut(s) 157, 343, 481, 652
Ppu21I YACGTR 1 cut(s) 127
PshAI GACNNNNGTC 1 cut(s) 536
PshBI ATTAAT 1 cut(s) 1074
PsiI TTATAA 2 cut(s) 275, 1200
Psp124BI GAGCTC 2 cut(s) 258, 741
Psp6I CCWGG 3 cut(s) 141, 369, 395
PspCI CACGTG 1 cut(s) 127
PspGI CCWGG 3 cut(s) 141, 369, 395
PspN4I GGNNCC 3 cut(s) 52, 211, 418
PspPI GGNCC 4 cut(s) 50, 139, 340, 615
PstI CTGCAG 2 cut(s) 447, 1141
PstNI CAGNNNCTG 1 cut(s) 442
PvuII CAGCTG 3 cut(s) 250, 869, 1019
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SacI GAGCTC 2 cut(s) 258, 741
SalI GTCGAC 1 cut(s) 646
SaqAI TTAA 2 cut(s) 222, 1074
Sau3AI GATC 3 cut(s) 459, 477, 1038
Sau96I GGNCC 4 cut(s) 50, 139, 340, 615
SchI GAGTC 4 cut(s) 157, 343, 481, 653
ScrFI CCNGG 3 cut(s) 143, 371, 397
SduI GDGCHC 4 cut(s) 174, 212, 258, 741
SfaNI GCATC 2 cut(s) 811, 1122
SfcI CTRYAG 2 cut(s) 443, 1137
SfiI GGCCNNNNNGGCC 1 cut(s) 371
SfoI GGCGCC 1 cut(s) 418
SinI GGWCC 1 cut(s) 139
SmlI CTYRAG 3 cut(s) 728, 742, 873
SmoI CTYRAG 3 cut(s) 728, 742, 873
Sse9I AATT 3 cut(s) 276, 914, 1300
SsiI CCGC 7 cut(s) 176, 362, 389, 452, 656, 778, 818
SspDI GGCGCC 1 cut(s) 416
SstI GAGCTC 2 cut(s) 258, 741
StyD4I CCNGG 3 cut(s) 141, 369, 395
StyI CCWWGG 3 cut(s) 375, 618, 651
TaaI ACNGT 4 cut(s) 300, 882, 1145, 1228
TaiI ACGT 3 cut(s) 129, 312, 387
TaqI TCGA 4 cut(s) 101, 153, 485, 647
TaqII GACCGA 1 cut(s) 734
TasI AATT 3 cut(s) 276, 914, 1300
TauI GCSGC 3 cut(s) 179, 365, 455
TfiI GAWTC 4 cut(s) 71, 553, 793, 1286
Tru1I TTAA 2 cut(s) 222, 1074
Tru9I TTAA 2 cut(s) 222, 1074
TscAI CASTG 1 cut(s) 1212
TseI GCWGC 9 cut(s) 94, 815, 869, 902, 1016, 1019, 1136, 1172, 1249
TspDTI ATGAA 5 cut(s) 13, 416, 614, 716, 1193
TspGWI ACGGA 4 cut(s) 9, 64, 548, 875
TspRI CASTG 1 cut(s) 1212
VpaK11BI GGWCC 1 cut(s) 139
VspI ATTAAT 1 cut(s) 1074
XapI RAATTY 1 cut(s) 914
XcmI CCANNNNNNNNNTGG 2 cut(s) 382, 589
XmiI GTMKAC 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.