Rmu_sc0021613.1_g000001

K( ) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0021613.1
OG0005607
Physical Location & Seq
Forward (+)
2197 .. 8122
5926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0021613.1_g000001.1.cds

Sequence Viewer

Length: 1647 bp
atgttgagaagaagccgtttcgcgattctgatctcaactctgctgcttctcagctccgatttgcttctctcactctcgctcgcagccgccgaccctctcgccgacgactcagatcccctcctcctcaacctcaacgccaccgagtccaatgtctcgctctccaagcccaaggaaggcagcttcgctgacatgatcgacaaggccctcgagaacgagttcaaggagaacgatcagaatcaagctactgatcctgggagttttaacaacagcgtcgccggccaacaggcaacactggaaacagttgccagagttaagtcgaagaaaaatgagacaaaagaggaaaacttaaataggtcgtttcaactgcatgatgtttttaatctggataatgataatggagcagaagagaccccaacactcatagatcgcaaggacaatgtctttataatatcaaactttaaatcaaaatatccagtgctgcagctagacctgagactgatatcagatctcgtcgtggttattgtatctgcaacctgtggtggcattgcttttgcttgtgctggacaaccggttatctctggatatttgctagctggatctgttattggacctggagggttcaacttcgtcagtgaaatggttcaagttgaaactgtagctcagtttggggtgatctttcttctttttgctttgggcttagagttctccacaacaaagcttcgagttgttcgagcagttgctgttccgggaggcctgctgcaaattttcctatttatgtgcctgtgtggtattacagcctcattatgtggtggtaaaatttctgagggggtattcgttggcgtgttcctttctatgtcttcaacagcagtggtgttaaaatttttgatggaaaaaaactccactaatgcgcttcacggtcaagtcaccattggcacccttattctgcaggattgtgctgtgggtttgctgtttgctttgcttcctgttctgggagggacttctggcatctttcaaggagtaatgtctatggctaagctgatggtgatgttgattacatttttggctgttctttctatatcatctcgtacttgtgttccttggttacttaaactgatgataagcttatcttcacagaccaatgaactctaccagttggcatcagtcgcattctgcttgcttgtagcctggtgtagtgacaagctgggattaagtctggaactgggttcctttgccgcaggagttatgatatcaacaactgatcttgctcaacatacactagaacaaattgaacccattcgcaacctttttgctgcccttttccttgccagcattgggatgctgattcatgttcagtttctctggaatcatgttgatatattactcgcgtctgtcatcttagttatcatcataaaaacaattattataaccatggttgtcaagggattcggatacaacaacaaaacatcattcctagttggaatatctctagcacagataggcgaatttgcttttgttcttttaagtcgtgcctctaatctccatctagtcgaggggaaattatatctgctgctgcttggaaccacagcacttagtctggtgaccactccttttctattcaagctaatccctattaatgagctttggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

548

Amino Acids

59.13

Weight (kDa)

5.39

Isoelectric Point (pI)

25.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 446, 1424
AccB1I GGYRCC 1 cut(s) 932
AccII CGCG 2 cut(s) 23, 1385
AciI CCGC 2 cut(s) 87, 1233
AclWI GGATC 3 cut(s) 107, 242, 604
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 4 cut(s) 762, 816, 878, 1502
AfaI GTAC 1 cut(s) 1087
AfiI CCNNNNNNNGG 3 cut(s) 173, 989, 1332
AgeI ACCGGT 1 cut(s) 568
AgsI TTSAA 9 cut(s) 220, 362, 622, 644, 650, 861, 1013, 1289, 1618
AjnI CCWGG 3 cut(s) 250, 610, 1184
Alw26I GTCTC 4 cut(s) 157, 323, 401, 487
AlwI GGATC 3 cut(s) 107, 242, 604
AlwNI CAGNNNCTG 1 cut(s) 740
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 3 cut(s) 201, 277, 751
ApeKI GCWGC 9 cut(s) 43, 83, 177, 478, 481, 757, 1310, 1567, 1570
ApoI RAATTY 4 cut(s) 762, 816, 878, 1502
AseI ATTAAT 1 cut(s) 1632
AsiGI ACCGGT 1 cut(s) 568
Asp700I GAANNNNTTC 3 cut(s) 215, 639, 1293
AspLEI GCGC 1 cut(s) 910
AspS9I GGNCC 2 cut(s) 202, 608
AsuC2I CCSGG 1 cut(s) 747
AsuHPI GGTGA 4 cut(s) 682, 916, 1054, 1609
AsuNHI GCTAGC 1 cut(s) 589
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 1 cut(s) 608
BanI GGYRCC 1 cut(s) 932
BbsI GAAGAC 1 cut(s) 849
BbvI GCAGC 9 cut(s) 30, 95, 189, 465, 493, 744, 1297, 1554, 1557
BccI CCATC 3 cut(s) 880, 1033, 1548
BciT130I CCWGG 3 cut(s) 252, 612, 1186
BciVI GTATCC 1 cut(s) 1442
BcnI CCSGG 1 cut(s) 747
BcoDI GTCTC 4 cut(s) 157, 323, 401, 487
BfaI CTAG 6 cut(s) 485, 590, 1277, 1472, 1487, 1544
BfmI CTRYAG 3 cut(s) 479, 654, 944
BfuI GTATCC 1 cut(s) 1442
BglII AGATCT 1 cut(s) 505
BlpI GCTNAGC 1 cut(s) 1032
Bme1390I CCNGG 4 cut(s) 252, 612, 747, 1186
Bme18I GGWCC 1 cut(s) 608
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 202, 608
BmiI GGNNCC 3 cut(s) 934, 1225, 1579
BmrFI CCNGG 4 cut(s) 252, 612, 747, 1186
BmrI ACTGGG 1 cut(s) 1229
BmsI GCATC 3 cut(s) 1014, 1166, 1326
BmtI GCTAGC 1 cut(s) 593
BmuI ACTGGG 1 cut(s) 1229
BpiI GAAGAC 1 cut(s) 849
BpmI CTGGAG 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 1032
BpuMI CCSGG 1 cut(s) 747
BsaBI GATNNNNATC 2 cut(s) 29, 234
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 4 cut(s) 168, 251, 1097, 1428
BsaWI WCCGGW 1 cut(s) 568
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 989, 1332
Bse118I RCCGGY 2 cut(s) 275, 568
Bse1I ACTGG 4 cut(s) 297, 473, 1150, 1224
Bse3DI GCAATG 1 cut(s) 543
Bse8I GATNNNNATC 2 cut(s) 29, 234
BseBI CCWGG 3 cut(s) 252, 612, 1186
BseDI CCNNGG 4 cut(s) 168, 251, 1097, 1428
BseGI GGATG 1 cut(s) 1341
BseJI GATNNNNATC 2 cut(s) 29, 234
BseLI CCNNNNNNNGG 3 cut(s) 173, 989, 1332
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 5 cut(s) 64, 123, 482, 674, 813
BseNI ACTGG 4 cut(s) 297, 473, 1150, 1224
BseRI GAGGAG 2 cut(s) 110, 113
BseXI GCAGC 9 cut(s) 30, 95, 189, 465, 493, 744, 1297, 1554, 1557
BseYI CCCAGC 1 cut(s) 1201
Bsh1236I CGCG 2 cut(s) 23, 1385
BshFI GGCC 3 cut(s) 203, 279, 753
BshNI GGYRCC 1 cut(s) 932
BshTI ACCGGT 1 cut(s) 568
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 3 cut(s) 276, 569, 746
BslFI GGGAC 1 cut(s) 1009
BslI CCNNNNNNNGG 3 cut(s) 173, 989, 1332
BsmAI GTCTC 4 cut(s) 157, 323, 401, 487
BsmFI GGGAC 1 cut(s) 1009
BsmI GAATGC 1 cut(s) 1166
BsnI GGCC 3 cut(s) 203, 279, 753
Bso31I GGTCTC 1 cut(s) 401
BsoBI CYCGRG 1 cut(s) 206
Bsp1720I GCTNAGC 1 cut(s) 1032
Bsp19I CCATGG 1 cut(s) 1428
Bsp68I TCGCGA 1 cut(s) 23
BspACI CCGC 2 cut(s) 87, 1233
BspANI GGCC 3 cut(s) 203, 279, 753
BspCNI CTCAG 5 cut(s) 63, 122, 483, 673, 814
BspFNI CGCG 2 cut(s) 23, 1385
BspLI GGNNCC 3 cut(s) 934, 1225, 1579
BspMAI CTGCAG 2 cut(s) 483, 948
BspOI GCTAGC 1 cut(s) 593
BspPI GGATC 3 cut(s) 107, 242, 604
BspT107I GGYRCC 1 cut(s) 932
BspTNI GGTCTC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 543
BsrFI RCCGGY 2 cut(s) 275, 568
BsrI ACTGG 4 cut(s) 297, 473, 1150, 1224
BssAI RCCGGY 2 cut(s) 275, 568
BssECI CCNNGG 4 cut(s) 168, 251, 1097, 1428
BssT1I CCWWGG 3 cut(s) 168, 1097, 1428
Bst2UI CCWGG 3 cut(s) 252, 612, 1186
Bst4CI ACNGT 3 cut(s) 301, 655, 917
Bst6I CTCTTC 1 cut(s) 399
BstC8I GCNNGC 6 cut(s) 81, 277, 591, 755, 1175, 1327
BstDEI CTNAG 9 cut(s) 50, 109, 491, 660, 697, 822, 1032, 1396, 1589
BstDSI CCRYGG 1 cut(s) 1428
BstEII GGTNACC 1 cut(s) 1597
BstF5I GGATG 1 cut(s) 1341
BstFNI CGCG 2 cut(s) 23, 1385
BstHHI GCGC 1 cut(s) 910
BstMAI GTCTC 4 cut(s) 157, 323, 401, 487
BstMWI GCNNNNNNNGC 3 cut(s) 163, 276, 1163
BstNI CCWGG 3 cut(s) 252, 612, 1186
BstPI GGTNACC 1 cut(s) 1597
BstSCI CCNGG 4 cut(s) 250, 610, 745, 1184
BstSFI CTRYAG 3 cut(s) 479, 654, 944
BstUI CGCG 2 cut(s) 23, 1385
BstV1I GCAGC 9 cut(s) 30, 95, 189, 465, 493, 744, 1297, 1554, 1557
BstV2I GAAGAC 1 cut(s) 849
BstX2I RGATCY 3 cut(s) 112, 505, 596
BstYI RGATCY 3 cut(s) 112, 505, 596
BsuI GTATCC 1 cut(s) 1442
BsuRI GGCC 3 cut(s) 203, 279, 753
BtgI CCRYGG 1 cut(s) 1428
BtsCI GGATG 1 cut(s) 1341
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 4 cut(s) 290, 480, 637, 873
BtuMI TCGCGA 1 cut(s) 23
Cac8I GCNNGC 6 cut(s) 81, 277, 591, 755, 1175, 1327
CaiI CAGNNNCTG 1 cut(s) 740
CfoI GCGC 1 cut(s) 910
Cfr10I RCCGGY 2 cut(s) 275, 568
Cfr13I GGNCC 2 cut(s) 202, 608
CseI GACGC 2 cut(s) 259, 1374
Csp6I GTAC 1 cut(s) 1086
CspAI ACCGGT 1 cut(s) 568
CspCI CAANNNNNGTGG 2 cut(s) 849, 884
CviAII CATG 5 cut(s) 190, 368, 1346, 1367, 1429
CviQI GTAC 1 cut(s) 1086
DdeI CTNAG 9 cut(s) 50, 109, 491, 660, 697, 822, 1032, 1396, 1589
DraI TTTAAA 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Eco130I CCWWGG 3 cut(s) 168, 1097, 1428
Eco147I AGGCCT 1 cut(s) 753
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 2 cut(s) 501, 1248
Eco47I GGWCC 1 cut(s) 608
Eco88I CYCGRG 1 cut(s) 206
Eco91I GGTNACC 1 cut(s) 1597
EcoO109I RGGNCCY 1 cut(s) 202
EcoO65I GGTNACC 1 cut(s) 1597
EcoRII CCWGG 3 cut(s) 250, 610, 1184
EcoRV GATATC 2 cut(s) 501, 1248
EcoT14I CCWWGG 3 cut(s) 168, 1097, 1428
ErhI CCWWGG 3 cut(s) 168, 1097, 1428
FaeI CATG 5 cut(s) 193, 371, 1349, 1370, 1432
FalI AAGNNNNNCTT 2 cut(s) 1111, 1143
FaqI GGGAC 1 cut(s) 1009
FatI CATG 5 cut(s) 189, 367, 1345, 1366, 1428
FokI GGATG 1 cut(s) 1348
FspBI CTAG 6 cut(s) 485, 590, 1277, 1472, 1487, 1544
GlaI GCGC 1 cut(s) 909
GsaI CCCAGC 1 cut(s) 1205
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 3 cut(s) 203, 279, 753
HapII CCGG 3 cut(s) 276, 569, 746
HgaI GACGC 2 cut(s) 259, 1374
HhaI GCGC 1 cut(s) 910
Hin1II CATG 5 cut(s) 193, 371, 1349, 1370, 1432
Hin6I GCGC 1 cut(s) 908
HinP1I GCGC 1 cut(s) 908
HindIII AAGCTT 2 cut(s) 716, 1120
HinfI GANTC 7 cut(s) 25, 107, 143, 235, 1342, 1363, 1443
HpaII CCGG 3 cut(s) 276, 569, 746
HphI GGTGA 4 cut(s) 682, 916, 1054, 1609
Hpy188I TCNGA 7 cut(s) 30, 58, 112, 234, 505, 823, 1448
Hpy188III TCNNGA 6 cut(s) 22, 208, 383, 579, 1214, 1360
Hpy99I CGWCG 3 cut(s) 107, 275, 515
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 3 cut(s) 301, 655, 917
HpyCH4V TGCA 5 cut(s) 367, 481, 530, 760, 946
HpyF10VI GCNNNNNNNGC 3 cut(s) 163, 276, 1163
HpyF3I CTNAG 9 cut(s) 50, 109, 491, 660, 697, 822, 1032, 1396, 1589
Hsp92II CATG 5 cut(s) 193, 371, 1349, 1370, 1432
HspAI GCGC 1 cut(s) 908
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
LmnI GCTCC 2 cut(s) 59, 398
Lsp1109I GCAGC 9 cut(s) 30, 95, 189, 465, 493, 744, 1297, 1554, 1557
LweI GCATC 3 cut(s) 1014, 1166, 1326
MaeI CTAG 6 cut(s) 485, 590, 1277, 1472, 1487, 1544
MaeIII GTNAC 4 cut(s) 922, 1101, 1193, 1597
MboII GAAGA 6 cut(s) 21, 331, 416, 671, 849, 1119
MflI RGATCY 3 cut(s) 112, 505, 596
MluCI AATT 7 cut(s) 762, 816, 878, 1284, 1416, 1502, 1556
MlyI GAGTC 2 cut(s) 101, 152
MmeI TCCRAC 1 cut(s) 1456
MroNI GCCGGC 1 cut(s) 275
MroXI GAANNNNTTC 3 cut(s) 215, 639, 1293
MslI CAYNNNNRTG 1 cut(s) 1334
MspI CCGG 3 cut(s) 276, 569, 746
MspR9I CCNGG 4 cut(s) 252, 612, 747, 1186
Mva1269I GAATGC 1 cut(s) 1166
MvaI CCWGG 3 cut(s) 252, 612, 1186
MvnI CGCG 2 cut(s) 23, 1385
MwoI GCNNNNNNNGC 3 cut(s) 163, 276, 1163
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 747
NcoI CCATGG 1 cut(s) 1428
NgoMIV GCCGGC 1 cut(s) 275
NheI GCTAGC 1 cut(s) 589
NlaIII CATG 5 cut(s) 193, 371, 1349, 1370, 1432
NlaIV GGNNCC 3 cut(s) 934, 1225, 1579
NmuCI GTSAC 3 cut(s) 922, 1193, 1597
NruI TCGCGA 1 cut(s) 23
PaeR7I CTCGAG 1 cut(s) 206
PceI AGGCCT 1 cut(s) 753
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 727
PctI GAATGC 1 cut(s) 1166
PdiI GCCGGC 1 cut(s) 277
PdmI GAANNNNTTC 3 cut(s) 215, 639, 1293
PfeI GAWTC 5 cut(s) 25, 235, 1342, 1363, 1443
PflFI GACNNNGTC 1 cut(s) 437
PfoI TCCNGGA 1 cut(s) 745
PinAI ACCGGT 1 cut(s) 568
PleI GAGTC 2 cut(s) 101, 151
PpsI GAGTC 2 cut(s) 101, 151
PshBI ATTAAT 1 cut(s) 1632
PsiI TTATAA 2 cut(s) 446, 1424
Psp6I CCWGG 3 cut(s) 250, 610, 1184
PspEI GGTNACC 1 cut(s) 1597
PspFI CCCAGC 1 cut(s) 1201
PspGI CCWGG 3 cut(s) 250, 610, 1184
PspN4I GGNNCC 3 cut(s) 934, 1225, 1579
PspPI GGNCC 2 cut(s) 202, 608
PstI CTGCAG 2 cut(s) 483, 948
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 3 cut(s) 112, 505, 596
PsyI GACNNNGTC 1 cut(s) 437
RruI TCGCGA 1 cut(s) 23
RsaI GTAC 1 cut(s) 1087
RsaNI GTAC 1 cut(s) 1086
RseI CAYNNNNRTG 1 cut(s) 1334
Sau96I GGNCC 2 cut(s) 202, 608
SchI GAGTC 2 cut(s) 101, 152
ScrFI CCNGG 4 cut(s) 252, 612, 747, 1186
SfaNI GCATC 3 cut(s) 1014, 1166, 1326
SfcI CTRYAG 3 cut(s) 479, 654, 944
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 1 cut(s) 608
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 1334
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 7 cut(s) 762, 816, 878, 1284, 1416, 1502, 1556
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 2 cut(s) 87, 1233
SspMI CTAG 6 cut(s) 485, 590, 1277, 1472, 1487, 1544
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 4 cut(s) 250, 610, 745, 1184
StyI CCWWGG 3 cut(s) 168, 1097, 1428
TaaI ACNGT 3 cut(s) 301, 655, 917
TaqI TCGA 6 cut(s) 195, 207, 317, 721, 730, 1548
TasI AATT 7 cut(s) 762, 816, 878, 1284, 1416, 1502, 1556
TauI GCSGC 2 cut(s) 89, 1235
TfiI GAWTC 5 cut(s) 25, 235, 1342, 1363, 1443
TscAI CASTG 4 cut(s) 297, 480, 637, 873
TseFI GTSAC 3 cut(s) 922, 1193, 1597
TseI GCWGC 9 cut(s) 43, 83, 177, 478, 481, 757, 1310, 1567, 1570
Tsp45I GTSAC 3 cut(s) 922, 1193, 1597
TspDTI ATGAA 2 cut(s) 1155, 1334
TspRI CASTG 4 cut(s) 297, 480, 637, 873
Tth111I GACNNNGTC 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 608
VspI ATTAAT 1 cut(s) 1632
XapI RAATTY 4 cut(s) 762, 816, 878, 1502
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 3 cut(s) 215, 639, 1293
XspI CTAG 6 cut(s) 485, 590, 1277, 1472, 1487, 1544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.