Rh6DG497900

K( ) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005607
Physical Location & Seq
Reverse (-)
65533781 .. 65541101
7321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG497900.1

Sequence Viewer

Length: 1614 bp
ATGTTGAGAAGAAGCCGTTTCGCGATTCTGATCTCAACTCTGCTGCTTCTCAGCTCCGATTTGCTTCTCTCACTCTCGCTCGCAGTCGCCGACCCTCTCGCCGACGACTCAGATCCCCTCCTCCTCAACCTCAACGCCACCGAGTCCAATGTCTCGCTCTCCAAGCCCAAGGAAGGCAGCTTCGCTGACATGATCGACAAGGCCCTCGAGAACGAGTTCAAGGAGAACGATCAGAATCAAGCTACTGATCCTGGGAGTTTTAACAACAGCGTCGCCGGCCAACAGGCAACACTGGAAACAGTTGCCAGAGTTAAGTCGAAGAAAAATGAGACAAAAGAGGAAAAGTCGTTTCAACTACATGATGTTTTTAATCTGGATAATGATAATGGAGCAGAAGAGACCCCAACACTCATAGATCGCAAGGACAATGTCTTTATAATATCGAACTTTAAATCAAAATATCCAGTGCTGCAGCTAGACCTGAGACTGATATCAGATCTCGTCGTGGTTATTGTATCTGCAACCTGTGGTGGCATTGCTTTTGCTTGTGCTGGACAACCGGTTATCTCTGGATATTTGCTAGCTGGATCTGTTATTGGACCGGGAGGGTTCAACTTCGTCAGTGAAATGGTTCAAGTTGAAACTGTAGCTCAGTTTGGGGTGATCTTTCTTCTTTTTGCTTTGGGCTTAGAGTTCTCCACAACAAAGCTTCGAGTTGTTCGAGCAGTTGCTGTTCCAGGAGGCCTGCTGCAAATTTTCCTATTTATGTGCCTGTGTGGTATTACAGCCTCATTATGTGGTGGTAAAATTTCTGAGGGGGTATTCGTTGGCGTGTTCCTTTCTATGTCTTCAACAGCAGTGGTGTTAAAATTTTTGATGGAAAAAAACTCCACTAATGCGCTTCACGGTCAAGTCACCATTGGCACCCTTATTCTGCAGGATTGTGCCGTGGGTTTGCTGTTTGCTTTGCTTCCTGTTCTGGGAGGGACTTCTGGCATCTTTCAAGGAGTAATGTCTATGGCTAAGCTGATGGTGATGTTGATTACATTTTTGGCTGTTCTTTCTATATCATCTCGTACTTGTGTTCCCTGGTTACTTAAACTGATGATAAGCTTATCTTCACAGACCAATGAACTCTACCAGTTGGCATCAGTCGCATTCTGCTTGCTTGTAGCCTGGTGTAGTGACAAGCTGGGATTAAGTCTGGAACTGGGTTCCTTTGCCGCAGGAGTTATGATATCAACAACTGATCTTGCTCAACATACACTAGAACAAATTGAACCCATTCGCAACCTTTTTGCTGCCCTTTTCCTTGCCAGCATCGGGATGCTGATTCATGTTCAGTTTCTCTGGAATCATGTTGATATATTACTCGCGTCTGTCATCTTAGTTATCATCATAAAAACAATTATTATAACCATGGTTGTCAAGGGATTTGGATACAACAACAAAACATCATTCCTAGTTGGAATATCTCTAGCACAGATAGGCGAATTTGCTTTTGTTCTTTTAAGTCGTGCCTCTAATCTCCATCTAGTCGAGGTTAGTGCTACCATTGGAGTTTCCACAATAATTGTTGTCCTCCTAAATAACTTATGGCCACAGTACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

537

Amino Acids

57.82

Weight (kDa)

5.27

Isoelectric Point (pI)

25.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 171 - 527 1.7e-70 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 437, 1415
AccB1I GGYRCC 1 cut(s) 923
AccII CGCG 2 cut(s) 23, 1376
AciI CCGC 1 cut(s) 1224
AclWI GGATC 3 cut(s) 107, 242, 595
AcoI YGGCCR 2 cut(s) 277, 1598
AcsI RAATTY 4 cut(s) 753, 807, 869, 1493
AfaI GTAC 2 cut(s) 1078, 1607
AfiI CCNNNNNNNGG 3 cut(s) 173, 980, 1323
AgeI ACCGGT 1 cut(s) 559
AgsI TTSAA 8 cut(s) 220, 353, 613, 635, 641, 852, 1004, 1280
AjnI CCWGG 4 cut(s) 250, 736, 1088, 1175
Alw26I GTCTC 4 cut(s) 157, 323, 392, 478
AlwI GGATC 3 cut(s) 107, 242, 595
AlwNI CAGNNNCTG 1 cut(s) 731
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 4 cut(s) 201, 277, 742, 1598
ApeKI GCWGC 6 cut(s) 43, 177, 469, 472, 748, 1301
ApoI RAATTY 4 cut(s) 753, 807, 869, 1493
AsiGI ACCGGT 1 cut(s) 559
Asp700I GAANNNNTTC 3 cut(s) 215, 630, 1284
AspLEI GCGC 1 cut(s) 901
AspS9I GGNCC 2 cut(s) 202, 599
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 3 cut(s) 673, 907, 1045
AsuNHI GCTAGC 1 cut(s) 580
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 1 cut(s) 599
BalI TGGCCA 1 cut(s) 1600
BanI GGYRCC 1 cut(s) 923
BbsI GAAGAC 1 cut(s) 840
BbvI GCAGC 6 cut(s) 30, 189, 456, 484, 735, 1288
BccI CCATC 3 cut(s) 871, 1024, 1539
BceAI ACGGC 1 cut(s) 932
BcgI CGANNNNNNTGC 2 cut(s) 1529, 1563
BciT130I CCWGG 4 cut(s) 252, 738, 1090, 1177
BciVI GTATCC 1 cut(s) 1433
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 4 cut(s) 157, 323, 392, 478
BfaI CTAG 6 cut(s) 476, 581, 1268, 1463, 1478, 1535
BfmI CTRYAG 3 cut(s) 470, 645, 935
BfuI GTATCC 1 cut(s) 1433
BglII AGATCT 1 cut(s) 496
BisI GCNGC 7 cut(s) 44, 178, 470, 473, 749, 1224, 1302
BlpI GCTNAGC 1 cut(s) 1023
BlsI GCNGC 7 cut(s) 45, 179, 471, 474, 750, 1225, 1303
Bme1390I CCNGG 5 cut(s) 252, 603, 738, 1090, 1177
Bme18I GGWCC 1 cut(s) 599
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 202, 599
BmiI GGNNCC 2 cut(s) 925, 1216
BmrFI CCNGG 5 cut(s) 252, 603, 738, 1090, 1177
BmrI ACTGGG 1 cut(s) 1220
BmsI GCATC 4 cut(s) 1005, 1157, 1317, 1329
BmtI GCTAGC 1 cut(s) 584
BmuI ACTGGG 1 cut(s) 1220
BpiI GAAGAC 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 1023
BpuMI CCSGG 1 cut(s) 603
BsaBI GATNNNNATC 2 cut(s) 29, 234
BsaI GGTCTC 1 cut(s) 392
BsaJI CCNNGG 5 cut(s) 168, 251, 948, 1088, 1419
BsaWI WCCGGW 1 cut(s) 559
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 980, 1323
Bse118I RCCGGY 2 cut(s) 275, 559
Bse1I ACTGG 4 cut(s) 297, 464, 1141, 1215
Bse3DI GCAATG 1 cut(s) 534
Bse8I GATNNNNATC 2 cut(s) 29, 234
BseBI CCWGG 4 cut(s) 252, 738, 1090, 1177
BseDI CCNNGG 5 cut(s) 168, 251, 948, 1088, 1419
BseGI GGATG 1 cut(s) 1332
BseJI GATNNNNATC 2 cut(s) 29, 234
BseLI CCNNNNNNNGG 3 cut(s) 173, 980, 1323
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 5 cut(s) 64, 123, 473, 665, 804
BseNI ACTGG 4 cut(s) 297, 464, 1141, 1215
BseRI GAGGAG 2 cut(s) 110, 113
BseXI GCAGC 6 cut(s) 30, 189, 456, 484, 735, 1288
BseYI CCCAGC 1 cut(s) 1192
Bsh1236I CGCG 2 cut(s) 23, 1376
BshFI GGCC 4 cut(s) 203, 279, 744, 1600
BshNI GGYRCC 1 cut(s) 923
BshTI ACCGGT 1 cut(s) 559
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 3 cut(s) 276, 560, 602
BslFI GGGAC 1 cut(s) 1000
BslI CCNNNNNNNGG 3 cut(s) 173, 980, 1323
BsmAI GTCTC 4 cut(s) 157, 323, 392, 478
BsmFI GGGAC 1 cut(s) 1000
BsmI GAATGC 1 cut(s) 1157
BsnI GGCC 4 cut(s) 203, 279, 744, 1600
Bso31I GGTCTC 1 cut(s) 392
BsoBI CYCGRG 1 cut(s) 206
Bsp1720I GCTNAGC 1 cut(s) 1023
Bsp19I CCATGG 1 cut(s) 1419
Bsp68I TCGCGA 1 cut(s) 23
BspACI CCGC 1 cut(s) 1224
BspANI GGCC 4 cut(s) 203, 279, 744, 1600
BspCNI CTCAG 5 cut(s) 63, 122, 474, 664, 805
BspFNI CGCG 2 cut(s) 23, 1376
BspLI GGNNCC 2 cut(s) 925, 1216
BspMAI CTGCAG 2 cut(s) 474, 939
BspOI GCTAGC 1 cut(s) 584
BspPI GGATC 3 cut(s) 107, 242, 595
BspT107I GGYRCC 1 cut(s) 923
BspTNI GGTCTC 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 534
BsrFI RCCGGY 2 cut(s) 275, 559
BsrI ACTGG 4 cut(s) 297, 464, 1141, 1215
BssAI RCCGGY 2 cut(s) 275, 559
BssECI CCNNGG 5 cut(s) 168, 251, 948, 1088, 1419
BssT1I CCWWGG 2 cut(s) 168, 1419
Bst2UI CCWGG 4 cut(s) 252, 738, 1090, 1177
Bst4CI ACNGT 4 cut(s) 301, 646, 908, 1605
Bst6I CTCTTC 1 cut(s) 390
BstC8I GCNNGC 6 cut(s) 81, 277, 582, 746, 1166, 1318
BstDEI CTNAG 8 cut(s) 50, 109, 482, 651, 688, 813, 1023, 1387
BstDSI CCRYGG 2 cut(s) 948, 1419
BstF5I GGATG 1 cut(s) 1332
BstFNI CGCG 2 cut(s) 23, 1376
BstHHI GCGC 1 cut(s) 901
BstMAI GTCTC 4 cut(s) 157, 323, 392, 478
BstMWI GCNNNNNNNGC 3 cut(s) 163, 276, 1154
BstNI CCWGG 4 cut(s) 252, 738, 1090, 1177
BstSCI CCNGG 5 cut(s) 250, 601, 736, 1088, 1175
BstSFI CTRYAG 3 cut(s) 470, 645, 935
BstUI CGCG 2 cut(s) 23, 1376
BstV1I GCAGC 6 cut(s) 30, 189, 456, 484, 735, 1288
BstV2I GAAGAC 1 cut(s) 840
BstX2I RGATCY 3 cut(s) 112, 496, 587
BstYI RGATCY 3 cut(s) 112, 496, 587
BsuI GTATCC 1 cut(s) 1433
BsuRI GGCC 4 cut(s) 203, 279, 744, 1600
BtgI CCRYGG 2 cut(s) 948, 1419
BtsCI GGATG 1 cut(s) 1332
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 4 cut(s) 290, 471, 628, 864
BtuMI TCGCGA 1 cut(s) 23
Cac8I GCNNGC 6 cut(s) 81, 277, 582, 746, 1166, 1318
CaiI CAGNNNCTG 1 cut(s) 731
CfoI GCGC 1 cut(s) 901
Cfr10I RCCGGY 2 cut(s) 275, 559
Cfr13I GGNCC 2 cut(s) 202, 599
CseI GACGC 2 cut(s) 259, 1365
Csp6I GTAC 2 cut(s) 1077, 1606
CspAI ACCGGT 1 cut(s) 559
CspCI CAANNNNNGTGG 4 cut(s) 840, 875, 1555, 1590
CviAII CATG 5 cut(s) 190, 359, 1337, 1358, 1420
CviQI GTAC 2 cut(s) 1077, 1606
DdeI CTNAG 8 cut(s) 50, 109, 482, 651, 688, 813, 1023, 1387
DraI TTTAAA 1 cut(s) 451
EaeI YGGCCR 2 cut(s) 277, 1598
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco130I CCWWGG 2 cut(s) 168, 1419
Eco147I AGGCCT 1 cut(s) 744
Eco31I GGTCTC 1 cut(s) 392
Eco32I GATATC 2 cut(s) 492, 1239
Eco47I GGWCC 1 cut(s) 599
Eco88I CYCGRG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 202
EcoRII CCWGG 4 cut(s) 250, 736, 1088, 1175
EcoRV GATATC 2 cut(s) 492, 1239
EcoT14I CCWWGG 2 cut(s) 168, 1419
ErhI CCWWGG 2 cut(s) 168, 1419
FaeI CATG 5 cut(s) 193, 362, 1340, 1361, 1423
FalI AAGNNNNNCTT 2 cut(s) 1102, 1134
FaqI GGGAC 1 cut(s) 1000
FatI CATG 5 cut(s) 189, 358, 1336, 1357, 1419
Fnu4HI GCNGC 7 cut(s) 44, 178, 470, 473, 749, 1224, 1302
FokI GGATG 1 cut(s) 1339
Fsp4HI GCNGC 7 cut(s) 44, 178, 470, 473, 749, 1224, 1302
FspBI CTAG 6 cut(s) 476, 581, 1268, 1463, 1478, 1535
GlaI GCGC 1 cut(s) 900
GluI GCNGC 7 cut(s) 44, 178, 470, 473, 749, 1224, 1302
GsaI CCCAGC 1 cut(s) 1196
HaeIII GGCC 4 cut(s) 203, 279, 744, 1600
HapII CCGG 3 cut(s) 276, 560, 602
HgaI GACGC 2 cut(s) 259, 1365
HhaI GCGC 1 cut(s) 901
Hin1II CATG 5 cut(s) 193, 362, 1340, 1361, 1423
Hin6I GCGC 1 cut(s) 899
HinP1I GCGC 1 cut(s) 899
HindIII AAGCTT 2 cut(s) 707, 1111
HinfI GANTC 6 cut(s) 25, 107, 143, 235, 1333, 1354
HpaII CCGG 3 cut(s) 276, 560, 602
HphI GGTGA 3 cut(s) 673, 907, 1045
Hpy188I TCNGA 6 cut(s) 30, 58, 112, 234, 496, 814
Hpy188III TCNNGA 7 cut(s) 22, 208, 374, 570, 1205, 1324, 1351
Hpy99I CGWCG 3 cut(s) 107, 275, 506
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 4 cut(s) 301, 646, 908, 1605
HpyCH4V TGCA 4 cut(s) 472, 521, 751, 937
HpyF10VI GCNNNNNNNGC 3 cut(s) 163, 276, 1154
HpyF3I CTNAG 8 cut(s) 50, 109, 482, 651, 688, 813, 1023, 1387
Hsp92II CATG 5 cut(s) 193, 362, 1340, 1361, 1423
HspAI GCGC 1 cut(s) 899
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
LmnI GCTCC 2 cut(s) 59, 389
Lsp1109I GCAGC 6 cut(s) 30, 189, 456, 484, 735, 1288
LweI GCATC 4 cut(s) 1005, 1157, 1317, 1329
MaeI CTAG 6 cut(s) 476, 581, 1268, 1463, 1478, 1535
MaeIII GTNAC 3 cut(s) 913, 1092, 1184
MboII GAAGA 6 cut(s) 21, 331, 407, 662, 840, 1110
MflI RGATCY 3 cut(s) 112, 496, 587
MlsI TGGCCA 1 cut(s) 1600
MluCI AATT 7 cut(s) 753, 807, 869, 1275, 1407, 1493, 1572
MluNI TGGCCA 1 cut(s) 1600
MlyI GAGTC 2 cut(s) 101, 152
MmeI TCCRAC 1 cut(s) 1447
Mox20I TGGCCA 1 cut(s) 1600
MroNI GCCGGC 1 cut(s) 275
MroXI GAANNNNTTC 3 cut(s) 215, 630, 1284
MscI TGGCCA 1 cut(s) 1600
MseI TTAA 8 cut(s) 261, 312, 369, 450, 866, 1098, 1199, 1511
MslI CAYNNNNRTG 1 cut(s) 1325
Msp20I TGGCCA 1 cut(s) 1600
MspI CCGG 3 cut(s) 276, 560, 602
MspR9I CCNGG 5 cut(s) 252, 603, 738, 1090, 1177
Mva1269I GAATGC 1 cut(s) 1157
MvaI CCWGG 4 cut(s) 252, 738, 1090, 1177
MvnI CGCG 2 cut(s) 23, 1376
MwoI GCNNNNNNNGC 3 cut(s) 163, 276, 1154
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 603
NcoI CCATGG 1 cut(s) 1419
NgoMIV GCCGGC 1 cut(s) 275
NheI GCTAGC 1 cut(s) 580
NlaIII CATG 5 cut(s) 193, 362, 1340, 1361, 1423
NlaIV GGNNCC 2 cut(s) 925, 1216
NmuCI GTSAC 2 cut(s) 913, 1184
NruI TCGCGA 1 cut(s) 23
PaeR7I CTCGAG 1 cut(s) 206
PceI AGGCCT 1 cut(s) 744
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 718
PctI GAATGC 1 cut(s) 1157
PdiI GCCGGC 1 cut(s) 277
PdmI GAANNNNTTC 3 cut(s) 215, 630, 1284
PfeI GAWTC 4 cut(s) 25, 235, 1333, 1354
PflFI GACNNNGTC 1 cut(s) 428
PfoI TCCNGGA 1 cut(s) 736
PinAI ACCGGT 1 cut(s) 559
PkrI GCNGC 7 cut(s) 45, 179, 471, 474, 750, 1225, 1303
PleI GAGTC 2 cut(s) 101, 151
PpsI GAGTC 2 cut(s) 101, 151
PsiI TTATAA 2 cut(s) 437, 1415
Psp6I CCWGG 4 cut(s) 250, 736, 1088, 1175
PspFI CCCAGC 1 cut(s) 1192
PspGI CCWGG 4 cut(s) 250, 736, 1088, 1175
PspN4I GGNNCC 2 cut(s) 925, 1216
PspPI GGNCC 2 cut(s) 202, 599
PstI CTGCAG 2 cut(s) 474, 939
PstNI CAGNNNCTG 1 cut(s) 731
PsuI RGATCY 3 cut(s) 112, 496, 587
PsyI GACNNNGTC 1 cut(s) 428
RruI TCGCGA 1 cut(s) 23
RsaI GTAC 2 cut(s) 1078, 1607
RsaNI GTAC 2 cut(s) 1077, 1606
RseI CAYNNNNRTG 1 cut(s) 1325
SaqAI TTAA 8 cut(s) 261, 312, 369, 450, 866, 1098, 1199, 1511
SatI GCNGC 7 cut(s) 44, 178, 470, 473, 749, 1224, 1302
Sau96I GGNCC 2 cut(s) 202, 599
SchI GAGTC 2 cut(s) 101, 152
ScrFI CCNGG 5 cut(s) 252, 603, 738, 1090, 1177
SfaNI GCATC 4 cut(s) 1005, 1157, 1317, 1329
SfcI CTRYAG 3 cut(s) 470, 645, 935
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 1 cut(s) 599
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 1325
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 7 cut(s) 753, 807, 869, 1275, 1407, 1493, 1572
SseBI AGGCCT 1 cut(s) 744
SsiI CCGC 1 cut(s) 1224
SspMI CTAG 6 cut(s) 476, 581, 1268, 1463, 1478, 1535
StuI AGGCCT 1 cut(s) 744
StyD4I CCNGG 5 cut(s) 250, 601, 736, 1088, 1175
StyI CCWWGG 2 cut(s) 168, 1419
TaaI ACNGT 4 cut(s) 301, 646, 908, 1605
TaqI TCGA 7 cut(s) 195, 207, 317, 443, 712, 721, 1539
TasI AATT 7 cut(s) 753, 807, 869, 1275, 1407, 1493, 1572
TauI GCSGC 1 cut(s) 1226
TfiI GAWTC 4 cut(s) 25, 235, 1333, 1354
Tru1I TTAA 8 cut(s) 261, 312, 369, 450, 866, 1098, 1199, 1511
Tru9I TTAA 8 cut(s) 261, 312, 369, 450, 866, 1098, 1199, 1511
TscAI CASTG 4 cut(s) 297, 471, 628, 864
TseFI GTSAC 2 cut(s) 913, 1184
TseI GCWGC 6 cut(s) 43, 177, 469, 472, 748, 1301
Tsp45I GTSAC 2 cut(s) 913, 1184
TspDTI ATGAA 2 cut(s) 1146, 1325
TspRI CASTG 4 cut(s) 297, 471, 628, 864
Tth111I GACNNNGTC 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 4 cut(s) 753, 807, 869, 1493
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 3 cut(s) 215, 630, 1284
XspI CTAG 6 cut(s) 476, 581, 1268, 1463, 1478, 1535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.