Rh6BG506500

K( ) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005607
Physical Location & Seq
Reverse (-)
70413781 .. 70419962
6182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG506500.1

Sequence Viewer

Length: 1323 bp
ATGTTGAGAAGAAGCCGTTTCGCGATTCTGATCTCAACTCTGCTGCTTCTCAGCTCCGATTTGCTTCTCTCACTCTCGCTCGCAGTCGCCGACCCTCTCGCCGACGACTCAGATCCCCTCCTCCTCAACCTCAACGCCACCGAGTCCAATGTCTCGCTCTCCAAGCCCAAGGAAGGCAGCTTCGCTGACATGATCGACAAGGCCCTCGAGAACGAGTTCAAGGAGAACGATCAGAATCAAGCTACTGATCCTGGGAGTTTTAACAACAGCGTCGCCGGCCAACAGGCAACACTGGAAACAGTTGCCAGAGTTAAGTCGAAGAAAAATGAGACAAAAGAGGAAAACTTAAATAGGTCGTTTCAACTACATGATGTTTTTAATCTGGATAATGATAATGGAGCAGAAGAGACCCCAACACTCATAGATCGCAAGGACAATGTCTTTATAATATCGAACTTTAAATCAAAATATCCAGTGCTGCAGCTAGACCTGAGACTGATATCAGATCTCGTCGTGGTTATTGTATCTGCAACCTGTGGTGGCATTGCTTTTGCTTGTGCTGGACAACCGGTTATCTCTGGATATTTGCTAGCTGGATCTGTTATTGGACCTGGAGGGTTCAACTTCGTCAGTGAAATGGTTCAAGTTGAAACTGTAGCTCAGTTTGGGGTGATCTTTCTTCTTTTTGCTTTGGGCTTAGAGTTCTCCACAACAAAGCTTCGAGTTGTTCGAGCAGTTGCTGTTCCAGGAGGCCTGCTGCAAATTTTCCTATTTATGTGCCTGTGTGGTATTACAGCCTCATTATGTGGTGGTAAAATTTCTGAGGGGGTATTCGTTGGCGTGTTCCTTTCTATGTCTTCAACAGCAGTGGTGTTAAAATTTTTGATGGAAAAAAACTCCACTAATGCGCTTCACGGTCAAGTCACCATTGGCACCCTTATTCTGCAGGATTGTGCCGTGGGTTTGCTGTTTGCTTTGCTTCCTGTTCTGGGAGGGACTTCTGGCATCTTTCAAGGAGTAATGTCTATGGCTAAGCTGATGGTGATGTTGATTACATTTTTGGCTGTTCTTTCTATATCATCTCGTACTTGTGTTCCCTGGTTACTTAAACTGATGATAAGCTTATCTTCACAGACCAATGAACTCTACCAGTTGGCATCAGTCGCATTCTGCTTGCTTGTAGCCTGGTGTAGTGACAAGCTGGGATTAAGTCTGGAACTGGGTTCCTTTGCCGCAGGAATTGGATTTAAGGGAGACAACCTGCGTACGGATAGTGGTAAACAGCGTGTTATTTTGATGGTCCGGGAATCACATGATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

440

Amino Acids

47.22

Weight (kDa)

5.18

Isoelectric Point (pI)

27.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 174 - 424 5e-49 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
Acc36I ACCTGC 1 cut(s) 1269
AccB1I GGYRCC 1 cut(s) 932
AccII CGCG 1 cut(s) 23
AciI CCGC 1 cut(s) 1233
AclWI GGATC 3 cut(s) 107, 242, 604
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 3 cut(s) 762, 816, 878
AfaI GTAC 2 cut(s) 1087, 1267
AfiI CCNNNNNNNGG 3 cut(s) 173, 989, 1267
AgeI ACCGGT 1 cut(s) 568
AgsI TTSAA 7 cut(s) 220, 362, 622, 644, 650, 861, 1013
AjnI CCWGG 5 cut(s) 250, 610, 745, 1097, 1184
Alw26I GTCTC 5 cut(s) 157, 323, 401, 487, 1248
AlwI GGATC 3 cut(s) 107, 242, 604
AlwNI CAGNNNCTG 1 cut(s) 740
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 3 cut(s) 201, 277, 751
ApeKI GCWGC 5 cut(s) 43, 177, 478, 481, 757
ApoI RAATTY 3 cut(s) 762, 816, 878
AsiGI ACCGGT 1 cut(s) 568
Asp700I GAANNNNTTC 2 cut(s) 215, 639
AspLEI GCGC 1 cut(s) 910
AspS9I GGNCC 3 cut(s) 202, 608, 1300
AsuC2I CCSGG 1 cut(s) 1304
AsuHPI GGTGA 3 cut(s) 682, 916, 1054
AsuNHI GCTAGC 1 cut(s) 589
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 2 cut(s) 608, 1300
BanI GGYRCC 1 cut(s) 932
BbsI GAAGAC 1 cut(s) 849
BbvI GCAGC 5 cut(s) 30, 189, 465, 493, 744
BccI CCATC 3 cut(s) 880, 1033, 1291
BceAI ACGGC 1 cut(s) 941
BciT130I CCWGG 5 cut(s) 252, 612, 747, 1099, 1186
BcnI CCSGG 1 cut(s) 1304
BcoDI GTCTC 5 cut(s) 157, 323, 401, 487, 1248
BfaI CTAG 2 cut(s) 485, 590
BfmI CTRYAG 3 cut(s) 479, 654, 944
BfuAI ACCTGC 1 cut(s) 1269
BglII AGATCT 1 cut(s) 505
BisI GCNGC 6 cut(s) 44, 178, 479, 482, 758, 1233
BlpI GCTNAGC 1 cut(s) 1032
BlsI GCNGC 6 cut(s) 45, 179, 480, 483, 759, 1234
Bme1390I CCNGG 6 cut(s) 252, 612, 747, 1099, 1186, 1304
Bme18I GGWCC 2 cut(s) 608, 1300
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 3 cut(s) 202, 608, 1300
BmiI GGNNCC 2 cut(s) 934, 1225
BmrFI CCNGG 6 cut(s) 252, 612, 747, 1099, 1186, 1304
BmrI ACTGGG 1 cut(s) 1229
BmsI GCATC 2 cut(s) 1014, 1166
BmtI GCTAGC 1 cut(s) 593
BmuI ACTGGG 1 cut(s) 1229
BpiI GAAGAC 1 cut(s) 849
BpmI CTGGAG 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 1032
BpuMI CCSGG 1 cut(s) 1304
BsaBI GATNNNNATC 2 cut(s) 29, 234
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 4 cut(s) 168, 251, 957, 1097
BsaWI WCCGGW 1 cut(s) 568
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 989, 1267
Bse118I RCCGGY 2 cut(s) 275, 568
Bse1I ACTGG 4 cut(s) 297, 473, 1150, 1224
Bse3DI GCAATG 1 cut(s) 543
Bse8I GATNNNNATC 2 cut(s) 29, 234
BseBI CCWGG 5 cut(s) 252, 612, 747, 1099, 1186
BseDI CCNNGG 4 cut(s) 168, 251, 957, 1097
BseJI GATNNNNATC 2 cut(s) 29, 234
BseLI CCNNNNNNNGG 3 cut(s) 173, 989, 1267
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 5 cut(s) 64, 123, 482, 674, 813
BseNI ACTGG 4 cut(s) 297, 473, 1150, 1224
BseRI GAGGAG 2 cut(s) 110, 113
BseXI GCAGC 5 cut(s) 30, 189, 465, 493, 744
BseYI CCCAGC 1 cut(s) 1201
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 203, 279, 753
BshNI GGYRCC 1 cut(s) 932
BshTI ACCGGT 1 cut(s) 568
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 3 cut(s) 276, 569, 1303
BsiWI CGTACG 1 cut(s) 1265
BslFI GGGAC 1 cut(s) 1009
BslI CCNNNNNNNGG 3 cut(s) 173, 989, 1267
BsmAI GTCTC 5 cut(s) 157, 323, 401, 487, 1248
BsmFI GGGAC 1 cut(s) 1009
BsmI GAATGC 1 cut(s) 1166
BsnI GGCC 3 cut(s) 203, 279, 753
Bso31I GGTCTC 1 cut(s) 401
BsoBI CYCGRG 1 cut(s) 206
Bsp143I GATC 9 cut(s) 30, 112, 192, 229, 247, 424, 505, 596, 672
Bsp1720I GCTNAGC 1 cut(s) 1032
Bsp68I TCGCGA 1 cut(s) 23
BspACI CCGC 1 cut(s) 1233
BspANI GGCC 3 cut(s) 203, 279, 753
BspCNI CTCAG 5 cut(s) 63, 122, 483, 673, 814
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 934, 1225
BspMAI CTGCAG 2 cut(s) 483, 948
BspMI ACCTGC 1 cut(s) 1269
BspOI GCTAGC 1 cut(s) 593
BspPI GGATC 3 cut(s) 107, 242, 604
BspT107I GGYRCC 1 cut(s) 932
BspTNI GGTCTC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 543
BsrFI RCCGGY 2 cut(s) 275, 568
BsrI ACTGG 4 cut(s) 297, 473, 1150, 1224
BssAI RCCGGY 2 cut(s) 275, 568
BssECI CCNNGG 4 cut(s) 168, 251, 957, 1097
BssMI GATC 9 cut(s) 30, 112, 192, 229, 247, 424, 505, 596, 672
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 5 cut(s) 252, 612, 747, 1099, 1186
Bst4CI ACNGT 3 cut(s) 301, 655, 917
Bst6I CTCTTC 1 cut(s) 399
BstC8I GCNNGC 5 cut(s) 81, 277, 591, 755, 1175
BstDEI CTNAG 7 cut(s) 50, 109, 491, 660, 697, 822, 1032
BstDSI CCRYGG 1 cut(s) 957
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 910
BstKTI GATC 9 cut(s) 33, 115, 195, 232, 250, 427, 508, 599, 675
BstMAI GTCTC 5 cut(s) 157, 323, 401, 487, 1248
BstMBI GATC 9 cut(s) 30, 112, 192, 229, 247, 424, 505, 596, 672
BstMWI GCNNNNNNNGC 3 cut(s) 163, 276, 1163
BstNI CCWGG 5 cut(s) 252, 612, 747, 1099, 1186
BstSCI CCNGG 6 cut(s) 250, 610, 745, 1097, 1184, 1302
BstSFI CTRYAG 3 cut(s) 479, 654, 944
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 5 cut(s) 30, 189, 465, 493, 744
BstV2I GAAGAC 1 cut(s) 849
BstX2I RGATCY 3 cut(s) 112, 505, 596
BstYI RGATCY 3 cut(s) 112, 505, 596
BsuRI GGCC 3 cut(s) 203, 279, 753
BtgI CCRYGG 1 cut(s) 957
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 4 cut(s) 290, 480, 637, 873
BtuMI TCGCGA 1 cut(s) 23
BveI ACCTGC 1 cut(s) 1269
Cac8I GCNNGC 5 cut(s) 81, 277, 591, 755, 1175
CaiI CAGNNNCTG 1 cut(s) 740
CfoI GCGC 1 cut(s) 910
Cfr10I RCCGGY 2 cut(s) 275, 568
Cfr13I GGNCC 3 cut(s) 202, 608, 1300
CseI GACGC 1 cut(s) 259
Csp6I GTAC 2 cut(s) 1086, 1266
CspAI ACCGGT 1 cut(s) 568
CspCI CAANNNNNGTGG 2 cut(s) 849, 884
CviAII CATG 3 cut(s) 190, 368, 1313
CviQI GTAC 2 cut(s) 1086, 1266
DdeI CTNAG 7 cut(s) 50, 109, 491, 660, 697, 822, 1032
DpnI GATC 9 cut(s) 32, 114, 194, 231, 249, 426, 507, 598, 674
DpnII GATC 9 cut(s) 30, 112, 192, 229, 247, 424, 505, 596, 672
DraI TTTAAA 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 168
Eco147I AGGCCT 1 cut(s) 753
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 2 cut(s) 608, 1300
Eco88I CYCGRG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 202
EcoRII CCWGG 5 cut(s) 250, 610, 745, 1097, 1184
EcoRV GATATC 1 cut(s) 501
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 3 cut(s) 193, 371, 1316
FalI AAGNNNNNCTT 2 cut(s) 1111, 1143
FaqI GGGAC 1 cut(s) 1009
FatI CATG 3 cut(s) 189, 367, 1312
Fnu4HI GCNGC 6 cut(s) 44, 178, 479, 482, 758, 1233
Fsp4HI GCNGC 6 cut(s) 44, 178, 479, 482, 758, 1233
FspBI CTAG 2 cut(s) 485, 590
GlaI GCGC 1 cut(s) 909
GluI GCNGC 6 cut(s) 44, 178, 479, 482, 758, 1233
GsaI CCCAGC 1 cut(s) 1205
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 3 cut(s) 203, 279, 753
HapII CCGG 3 cut(s) 276, 569, 1303
HgaI GACGC 1 cut(s) 259
HhaI GCGC 1 cut(s) 910
Hin1II CATG 3 cut(s) 193, 371, 1316
Hin6I GCGC 1 cut(s) 908
HinP1I GCGC 1 cut(s) 908
HindIII AAGCTT 2 cut(s) 716, 1120
HinfI GANTC 6 cut(s) 25, 107, 143, 235, 1307, 1316
HpaII CCGG 3 cut(s) 276, 569, 1303
HphI GGTGA 3 cut(s) 682, 916, 1054
Hpy166II GTNNAC 1 cut(s) 1280
Hpy188I TCNGA 6 cut(s) 30, 58, 112, 234, 505, 823
Hpy188III TCNNGA 5 cut(s) 22, 208, 383, 579, 1214
Hpy8I GTNNAC 1 cut(s) 1280
Hpy99I CGWCG 3 cut(s) 107, 275, 515
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 3 cut(s) 301, 655, 917
HpyCH4V TGCA 4 cut(s) 481, 530, 760, 946
HpyF10VI GCNNNNNNNGC 3 cut(s) 163, 276, 1163
HpyF3I CTNAG 7 cut(s) 50, 109, 491, 660, 697, 822, 1032
Hsp92II CATG 3 cut(s) 193, 371, 1316
HspAI GCGC 1 cut(s) 908
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
Kzo9I GATC 9 cut(s) 30, 112, 192, 229, 247, 424, 505, 596, 672
LmnI GCTCC 2 cut(s) 59, 398
Lsp1109I GCAGC 5 cut(s) 30, 189, 465, 493, 744
LweI GCATC 2 cut(s) 1014, 1166
MaeI CTAG 2 cut(s) 485, 590
MaeIII GTNAC 3 cut(s) 922, 1101, 1193
MalI GATC 9 cut(s) 32, 114, 194, 231, 249, 426, 507, 598, 674
MboI GATC 9 cut(s) 30, 112, 192, 229, 247, 424, 505, 596, 672
MboII GAAGA 6 cut(s) 21, 331, 416, 671, 849, 1119
MflI RGATCY 3 cut(s) 112, 505, 596
MluCI AATT 4 cut(s) 762, 816, 878, 1239
MlyI GAGTC 2 cut(s) 101, 152
MroNI GCCGGC 1 cut(s) 275
MroXI GAANNNNTTC 2 cut(s) 215, 639
MseI TTAA 9 cut(s) 261, 312, 347, 378, 459, 875, 1107, 1208, 1248
MspI CCGG 3 cut(s) 276, 569, 1303
MspR9I CCNGG 6 cut(s) 252, 612, 747, 1099, 1186, 1304
Mva1269I GAATGC 1 cut(s) 1166
MvaI CCWGG 5 cut(s) 252, 612, 747, 1099, 1186
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 163, 276, 1163
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 1304
NdeII GATC 9 cut(s) 30, 112, 192, 229, 247, 424, 505, 596, 672
NgoMIV GCCGGC 1 cut(s) 275
NheI GCTAGC 1 cut(s) 589
NlaIII CATG 3 cut(s) 193, 371, 1316
NlaIV GGNNCC 2 cut(s) 934, 1225
NmuCI GTSAC 2 cut(s) 922, 1193
NruI TCGCGA 1 cut(s) 23
PaeR7I CTCGAG 1 cut(s) 206
PceI AGGCCT 1 cut(s) 753
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 727
PctI GAATGC 1 cut(s) 1166
PdiI GCCGGC 1 cut(s) 277
PdmI GAANNNNTTC 2 cut(s) 215, 639
PfeI GAWTC 4 cut(s) 25, 235, 1307, 1316
Pfl23II CGTACG 1 cut(s) 1265
PflFI GACNNNGTC 1 cut(s) 437
PfoI TCCNGGA 2 cut(s) 745, 1302
PinAI ACCGGT 1 cut(s) 568
PkrI GCNGC 6 cut(s) 45, 179, 480, 483, 759, 1234
PleI GAGTC 2 cut(s) 101, 151
PpsI GAGTC 2 cut(s) 101, 151
PsiI TTATAA 1 cut(s) 446
Psp6I CCWGG 5 cut(s) 250, 610, 745, 1097, 1184
PspFI CCCAGC 1 cut(s) 1201
PspGI CCWGG 5 cut(s) 250, 610, 745, 1097, 1184
PspLI CGTACG 1 cut(s) 1265
PspN4I GGNNCC 2 cut(s) 934, 1225
PspPI GGNCC 3 cut(s) 202, 608, 1300
PstI CTGCAG 2 cut(s) 483, 948
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 3 cut(s) 112, 505, 596
PsyI GACNNNGTC 1 cut(s) 437
RruI TCGCGA 1 cut(s) 23
RsaI GTAC 2 cut(s) 1087, 1267
RsaNI GTAC 2 cut(s) 1086, 1266
SaqAI TTAA 9 cut(s) 261, 312, 347, 378, 459, 875, 1107, 1208, 1248
SatI GCNGC 6 cut(s) 44, 178, 479, 482, 758, 1233
Sau3AI GATC 9 cut(s) 30, 112, 192, 229, 247, 424, 505, 596, 672
Sau96I GGNCC 3 cut(s) 202, 608, 1300
SchI GAGTC 2 cut(s) 101, 152
ScrFI CCNGG 6 cut(s) 252, 612, 747, 1099, 1186, 1304
SfaNI GCATC 2 cut(s) 1014, 1166
SfcI CTRYAG 3 cut(s) 479, 654, 944
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 2 cut(s) 608, 1300
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 4 cut(s) 762, 816, 878, 1239
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 1 cut(s) 1233
SspMI CTAG 2 cut(s) 485, 590
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 6 cut(s) 250, 610, 745, 1097, 1184, 1302
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 3 cut(s) 301, 655, 917
TaqI TCGA 6 cut(s) 195, 207, 317, 452, 721, 730
TasI AATT 4 cut(s) 762, 816, 878, 1239
TauI GCSGC 1 cut(s) 1235
TfiI GAWTC 4 cut(s) 25, 235, 1307, 1316
Tru1I TTAA 9 cut(s) 261, 312, 347, 378, 459, 875, 1107, 1208, 1248
Tru9I TTAA 9 cut(s) 261, 312, 347, 378, 459, 875, 1107, 1208, 1248
TscAI CASTG 4 cut(s) 297, 480, 637, 873
TseFI GTSAC 2 cut(s) 922, 1193
TseI GCWGC 5 cut(s) 43, 177, 478, 481, 757
Tsp45I GTSAC 2 cut(s) 922, 1193
TspDTI ATGAA 2 cut(s) 1155, 1308
TspGWI ACGGA 1 cut(s) 1283
TspRI CASTG 4 cut(s) 297, 480, 637, 873
Tth111I GACNNNGTC 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 608, 1300
XapI RAATTY 3 cut(s) 762, 816, 878
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 2 cut(s) 215, 639
XspI CTAG 2 cut(s) 485, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.