pycom08g11450

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006494
Physical Location & Seq
Reverse (-)
9668372 .. 9673292
4921 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2271 bp
ATGTACGACTCTCAGCCTCTTCCGGTCATCATCCGCAACGCTTACTATCATCAATTTCTCTCTCGGTTCCCAATTTCTAGGGTTTCTTCTCTGCCCGCTTGTGGATGCATACATAGATTCGAGTTGCTGCACTGGTTATATAATCAGGGGCCGCAATATGTTTGCACGACCTTCTTGACATTTGTCTGTACAAAGTGTAGTGGAGTACATCGGGAGTTCACTCACCGAGTAAAATCAGTGTCAATGGCTAAATTTAATGCAGAAGAAGTTAGTGCACTTCAAGCAGGGGGAAATGAGCGAGCAAGACAAATTTATTTTAAAGAATGGGATCCTCAGTACCATTCTTACCCTGATGCCGGTAATCTTCATGGACTCAGGAGTTTTATAAAGCATGTATATGTTGATAGAAAATACACTGGCGAGAGGAGTGTCTACACTGGTGAGAGGAGTATCTACACTAGTGAGAGGAGTGGCAACAAGCTCCCAATGCTGCGATTGAGCACTGAGGAGTCTGATGAAAACCAGAAGGTTGGTGGACATCGTAGTGGATCCAGGAGCTTCCATGATGATGACAGGTTGGAACGGAGTTATAGTAATAGTCCAGGTGGAAGAAGTTTAAGATACTACTATGATGAGAGAAGAAGTCCTCGATATTCTCCAGAAAACTCAAGAAGTGGTGGTTTTATAAGAGCTCCTCTACGTTTTGAGATTGTTGATAACAGGATTCGGGGGGGAAAACGTGGATCCTCAAGTGGAGAATCCAAGGTCCAAAGCAGGACACCAGACAATAAGAAGACCACAGAAGGGTCCCATTCTCCTGTTGTGCCCCCTGTTAAAGATAAACTACGAGAAAACGTGCCTCCTCCACAGGTTGGTGAAAGTTCCACAGCAAATCAGAAGGATACTGATGGTTCTGCTCATAATCAGAAAACCAAATCTACTGGTGGCCATGATGGGAATGCGACCAAACAAAATATAGTAAGCTTGATTGATTTCAATACTGATTCCGAGCCCCCACATGCTACAGAATCACAATTGCAGCAAACACCTCCACCCAATGAGGATAACAACTGGGCCTCATTTGGATCATCTACAACGGAGAAGGCACCTCAAGTTCCAACTGCGGTTCCAAATGCAGACCCTCTGGAGTCTTTGCTATTTGAATTGTCAACTCCCTCATCTGTTCCTGTGAGTAATGCATCTGAGACACCACACAATGATGGTGCTCCGTCAACTGCATCTATAAACACCATGCCTGCAGGTGGTCATTCCCCAGCTGATGCAGGTCCTGTGCAAGATCTTGCTATTTTTGGTGGTGACACCACTGTAAAAGGTACTGATGAGAAGCAGTTAGTACTAAGTACACAGCAAGATCAATCTTCTATATCCTTCACCACGGATAGCGGTTCTACTTCACATGTCAATTTTGCAGTTGGAGCTTCAAACGCTGAGTTGAGGAACTTATCACTGGCACCAAGCATACAAAGATCTTTCAGTATTCCTGCTGAAAAATCATCTCAATCTATTTTGAATCCAGCTGAACATACCGACAGTGGCAGTGGAGCTGGATCCCAAGTGGAAATGAATACTAGCGACCTTTTTACTGCAAGTTACTCATCAGCCCCTGGACAAGCTTCAGGCTGGCATATTGCTCCAGTTCAGGGAATGGGGTACAACATGCAGTATTACCCTACCGCGACGGTATATAATCCGGCTCCAGTAATTCCGGGTCCAGCAAAACCAAAAAATCCGTTTGATCTTAATGAAGAAAAAAGCCTAGTGACTTCCACACATGTATGCACAACATTCAATTTGATACCTCTTTTTCCTTCCATGTCTTCATTGCAAGGAGCTCTGCCTAATGCTCCAGTACCAGGCTTGATGCATGCTTCGAGCCTTCCTACTATGTCGCAATTTGAGTCTAATGAATCCATGGCGATGCCTTCACACTCGCCTTCGTTCGCAAATGCTTTCTCTCCGAAGCATGTGCTACTAGAACACCGTGTCTCTCCTTCCTTCCCTTCTCCCTCTCTTATGTGTTTGTTATTCTCCATTTCGTCTGTAATTATGTGCTCAGTTTCTCTACTTTTCGGTCATTGTCATCATTGCAGACCACAGGGAGTTGGCGGCTTTGGCAGGGACGAAGCTGCTTTTGGATCCCTAACCACAACTCAGCAATCATTTCAGCAACCAAGCGTCAGATACCCAGCACCCAGCAGCAGCAGCACCTTAGACACTTTTTCTCCAATGAGAGGAAACCCATTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArfGap

Protein Analysis

757

Amino Acids

82.13

Weight (kDa)

6.62

Isoelectric Point (pI)

64.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 49 - 139 1.3e-20 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 386, 686
AarI CACCTGC 1 cut(s) 1250
Acc36I ACCTGC 2 cut(s) 1250, 1274
AccB1I GGYRCC 2 cut(s) 1105, 1471
AccB7I CCANNNNNTGG 1 cut(s) 872
AccI GTMKAC 1 cut(s) 432
AccII CGCG 1 cut(s) 1697
AciI CCGC 7 cut(s) 34, 96, 152, 1124, 1404, 1695, 2127
AcoI YGGCCR 1 cut(s) 946
AcsI RAATTY 2 cut(s) 251, 309
AcuI CTGAAG 1 cut(s) 1620
AfaI GTAC 9 cut(s) 5, 190, 207, 338, 1336, 1356, 1363, 1673, 1872
AfiI CCNNNNNNNGG 8 cut(s) 101, 356, 804, 872, 1262, 1661, 1874, 2252
AflIII ACRYGT 2 cut(s) 1417, 1792
AgsI TTSAA 6 cut(s) 281, 997, 1163, 1443, 1531, 1810
AhlI ACTAGT 1 cut(s) 458
AjnI CCWGG 4 cut(s) 551, 601, 1624, 1873
AjuI GAANNNNNNNTTGG 2 cut(s) 2136, 2168
Alw21I GWGCWC 6 cut(s) 277, 503, 694, 1228, 1855, 2075
Alw26I GTCTC 2 cut(s) 1199, 2011
Alw44I GTGCAC 1 cut(s) 273
AlwNI CAGNNNCTG 2 cut(s) 1289, 1625
AoxI GGCC 3 cut(s) 149, 946, 1074
ApaLI GTGCAC 1 cut(s) 273
ApeKI GCWGC 7 cut(s) 127, 490, 1039, 2147, 2217, 2220, 2223
ApoI RAATTY 2 cut(s) 251, 309
AspS9I GGNCC 6 cut(s) 149, 766, 807, 1074, 1286, 1730
AsuC2I CCSGG 1 cut(s) 1728
AsuHPI GGTGA 5 cut(s) 215, 452, 887, 1328, 1384
AvaII GGWCC 4 cut(s) 766, 807, 1286, 1730
BaeGI GKGCMC 2 cut(s) 277, 828
BalI TGGCCA 1 cut(s) 948
BamHI GGATCC 5 cut(s) 328, 548, 743, 1568, 2156
BanI GGYRCC 2 cut(s) 1105, 1471
BanII GRGCYC 3 cut(s) 694, 1014, 1855
BbsI GAAGAC 2 cut(s) 800, 1830
Bbv12I GWGCWC 6 cut(s) 277, 503, 694, 1228, 1855, 2075
BbvI GCAGC 7 cut(s) 114, 477, 1051, 2134, 2229, 2232, 2235
BccI CCATC 3 cut(s) 902, 947, 1214
BcgI CGANNNNNNTGC 2 cut(s) 1969, 2003
BciT130I CCWGG 4 cut(s) 553, 603, 1626, 1875
BciVI GTATCC 1 cut(s) 895
BcnI CCSGG 1 cut(s) 1728
BcoDI GTCTC 2 cut(s) 1199, 2011
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 5 cut(s) 78, 459, 1590, 1778, 1994
BfmI CTRYAG 2 cut(s) 1023, 1257
BfuAI ACCTGC 2 cut(s) 1250, 1274
BfuI GTATCC 1 cut(s) 895
BglII AGATCT 2 cut(s) 1297, 1487
BisI GCNGC 9 cut(s) 128, 152, 491, 1040, 2128, 2148, 2218, 2221, 2224
BlsI GCNGC 9 cut(s) 129, 153, 492, 1041, 2129, 2149, 2219, 2222, 2225
BmcAI AGTACT 1 cut(s) 1356
Bme1390I CCNGG 5 cut(s) 553, 603, 1626, 1728, 1875
Bme18I GGWCC 4 cut(s) 766, 807, 1286, 1730
BmgT120I GGNCC 6 cut(s) 149, 766, 807, 1074, 1286, 1730
BmrFI CCNGG 5 cut(s) 553, 603, 1626, 1728, 1875
BmrI ACTGGG 1 cut(s) 1081
BmsI GCATC 7 cut(s) 95, 343, 1208, 1247, 1270, 1872, 1929
BmuI ACTGGG 1 cut(s) 1081
BoxI GACNNNNGTC 1 cut(s) 182
BpiI GAAGAC 2 cut(s) 800, 1830
BpmI CTGGAG 5 cut(s) 642, 1166, 1638, 1701, 1851
BpuEI CTTGAG 3 cut(s) 652, 733, 1095
BpuMI CCSGG 1 cut(s) 1728
BsaJI CCNNGG 4 cut(s) 762, 1395, 1624, 1932
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 4 cut(s) 577, 607, 2227, 2257
Bsc4I CCNNNNNNNGG 8 cut(s) 101, 356, 804, 872, 1262, 1661, 1874, 2252
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 9 cut(s) 137, 421, 442, 946, 1076, 1473, 1655, 1718, 1868
Bse3DI GCAATG 2 cut(s) 1841, 2104
BseBI CCWGG 4 cut(s) 553, 603, 1626, 1875
BseDI CCNNGG 4 cut(s) 762, 1395, 1624, 1932
BseGI GGATG 2 cut(s) 30, 110
BseLI CCNNNNNNNGG 8 cut(s) 101, 356, 804, 872, 1262, 1661, 1874, 2252
BseMI GCAATG 2 cut(s) 1841, 2104
BseMII CTCAG 8 cut(s) 26, 347, 388, 495, 1194, 1440, 2088, 2186
BseNI ACTGG 9 cut(s) 137, 421, 442, 946, 1076, 1473, 1655, 1718, 1868
BseRI GAGGAG 6 cut(s) 439, 460, 481, 521, 684, 852
BseSI GKGCMC 2 cut(s) 277, 828
BseXI GCAGC 7 cut(s) 114, 477, 1051, 2134, 2229, 2232, 2235
BseYI CCCAGC 3 cut(s) 1273, 2206, 2213
BsgI GTGCAG 1 cut(s) 113
Bsh1236I CGCG 1 cut(s) 1697
BshFI GGCC 3 cut(s) 151, 948, 1076
BshNI GGYRCC 2 cut(s) 1105, 1471
BsiHKAI GWGCWC 6 cut(s) 277, 503, 694, 1228, 1855, 2075
BsiSI CCGG 4 cut(s) 23, 357, 1712, 1727
BslFI GGGAC 2 cut(s) 793, 2153
BslI CCNNNNNNNGG 8 cut(s) 101, 356, 804, 872, 1262, 1661, 1874, 2252
BsmAI GTCTC 2 cut(s) 1199, 2011
BsmFI GGGAC 2 cut(s) 793, 2153
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 3 cut(s) 151, 948, 1076
Bsp1286I GDGCHC 8 cut(s) 277, 503, 694, 828, 1014, 1228, 1855, 2075
Bsp1407I TGTACA 1 cut(s) 188
Bsp19I CCATGG 1 cut(s) 1932
BspACI CCGC 7 cut(s) 34, 96, 152, 1124, 1404, 1695, 2127
BspANI GGCC 3 cut(s) 151, 948, 1076
BspCNI CTCAG 8 cut(s) 25, 346, 387, 496, 1195, 1441, 2087, 2185
BspFNI CGCG 1 cut(s) 1697
BspMAI CTGCAG 1 cut(s) 1261
BspMI ACCTGC 2 cut(s) 1250, 1274
BspT107I GGYRCC 2 cut(s) 1105, 1471
BsrDI GCAATG 2 cut(s) 1841, 2104
BsrFI RCCGGY 1 cut(s) 356
BsrGI TGTACA 1 cut(s) 188
BsrI ACTGG 9 cut(s) 137, 421, 442, 946, 1076, 1473, 1655, 1718, 1868
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 4 cut(s) 762, 1395, 1624, 1932
BssT1I CCWWGG 2 cut(s) 762, 1932
Bst2UI CCWGG 4 cut(s) 553, 603, 1626, 1875
Bst4CI ACNGT 4 cut(s) 1327, 1553, 1702, 2003
Bst6I CTCTTC 1 cut(s) 24
BstAUI TGTACA 1 cut(s) 188
BstC8I GCNNGC 5 cut(s) 96, 300, 1257, 1643, 1887
BstDSI CCRYGG 2 cut(s) 1395, 1932
BstF5I GGATG 2 cut(s) 30, 110
BstFNI CGCG 1 cut(s) 1697
BstMAI GTCTC 2 cut(s) 1199, 2011
BstMWI GCNNNNNNNGC 6 cut(s) 281, 487, 1436, 1445, 2133, 2223
BstNI CCWGG 4 cut(s) 553, 603, 1626, 1875
BstNSI RCATGY 7 cut(s) 395, 1022, 1421, 1681, 1796, 1889, 1988
BstPAI GACNNNNGTC 1 cut(s) 182
BstSCI CCNGG 5 cut(s) 551, 601, 1624, 1726, 1873
BstSFI CTRYAG 2 cut(s) 1023, 1257
BstSLI GKGCMC 2 cut(s) 277, 828
BstUI CGCG 1 cut(s) 1697
BstV1I GCAGC 7 cut(s) 114, 477, 1051, 2134, 2229, 2232, 2235
BstV2I GAAGAC 2 cut(s) 800, 1830
BstX2I RGATCY 7 cut(s) 328, 548, 743, 1297, 1487, 1568, 2156
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 7 cut(s) 328, 548, 743, 1297, 1487, 1568, 2156
BsuI GTATCC 1 cut(s) 895
BsuRI GGCC 3 cut(s) 151, 948, 1076
BtgI CCRYGG 2 cut(s) 1395, 1932
BtgZI GCGATG 1 cut(s) 1952
BtsCI GGATG 2 cut(s) 30, 110
BtsI GCAGTG 1 cut(s) 1564
BtsIMutI CAGTG 9 cut(s) 130, 243, 414, 435, 501, 1323, 1466, 1558, 1564
BveI ACCTGC 2 cut(s) 1250, 1274
Cac8I GCNNGC 5 cut(s) 96, 300, 1257, 1643, 1887
CaiI CAGNNNCTG 2 cut(s) 1289, 1625
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 6 cut(s) 149, 766, 807, 1074, 1286, 1730
CseI GACGC 1 cut(s) 2185
Csp6I GTAC 9 cut(s) 4, 189, 206, 337, 1335, 1355, 1362, 1672, 1871
CviQI GTAC 9 cut(s) 4, 189, 206, 337, 1335, 1355, 1362, 1672, 1871
DraI TTTAAA 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 946
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Ecl136II GAGCTC 2 cut(s) 692, 1853
Eco130I CCWWGG 2 cut(s) 762, 1932
Eco24I GRGCYC 3 cut(s) 694, 1014, 1855
Eco47I GGWCC 4 cut(s) 766, 807, 1286, 1730
Eco53kI GAGCTC 2 cut(s) 692, 1853
Eco57I CTGAAG 1 cut(s) 1620
EcoICRI GAGCTC 2 cut(s) 692, 1853
EcoO109I RGGNCCY 2 cut(s) 807, 1286
EcoRII CCWGG 4 cut(s) 551, 601, 1624, 1873
EcoT14I CCWWGG 2 cut(s) 762, 1932
EcoT22I ATGCAT 3 cut(s) 110, 1201, 1887
EcoT38I GRGCYC 3 cut(s) 694, 1014, 1855
ErhI CCWWGG 2 cut(s) 762, 1932
FaqI GGGAC 2 cut(s) 793, 2153
FauI CCCGC 1 cut(s) 103
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 9 cut(s) 128, 152, 491, 1040, 2128, 2148, 2218, 2221, 2224
FokI GGATG 2 cut(s) 17, 117
FriOI GRGCYC 3 cut(s) 694, 1014, 1855
Fsp4HI GCNGC 9 cut(s) 128, 152, 491, 1040, 2128, 2148, 2218, 2221, 2224
FspBI CTAG 5 cut(s) 78, 459, 1590, 1778, 1994
GluI GCNGC 9 cut(s) 128, 152, 491, 1040, 2128, 2148, 2218, 2221, 2224
GsaI CCCAGC 3 cut(s) 1277, 2210, 2217
GsuI CTGGAG 5 cut(s) 642, 1166, 1638, 1701, 1851
HaeIII GGCC 3 cut(s) 151, 948, 1076
HapII CCGG 4 cut(s) 23, 357, 1712, 1727
HgaI GACGC 1 cut(s) 2185
HincII GTYRAC 2 cut(s) 1170, 1233
HindII GTYRAC 2 cut(s) 1170, 1233
HindIII AAGCTT 2 cut(s) 982, 1632
HpaII CCGG 4 cut(s) 23, 357, 1712, 1727
HphI GGTGA 5 cut(s) 215, 452, 887, 1328, 1384
Hpy166II GTNNAC 7 cut(s) 219, 275, 433, 536, 1170, 1233, 1364
Hpy188I TCNGA 7 cut(s) 514, 897, 927, 1009, 1204, 1980, 2201
Hpy188III TCNNGA 6 cut(s) 175, 212, 376, 659, 669, 1145
Hpy8I GTNNAC 7 cut(s) 219, 275, 433, 536, 1170, 1233, 1364
Hpy99I CGWCG 1 cut(s) 1702
HpyCH4III ACNGT 4 cut(s) 1327, 1553, 1702, 2003
HpyCH4IV ACGT 3 cut(s) 700, 739, 855
HpyF10VI GCNNNNNNNGC 6 cut(s) 281, 487, 1436, 1445, 2133, 2223
HpySE526I ACGT 3 cut(s) 700, 739, 855
KflI GGGWCCC 1 cut(s) 807
Lsp1109I GCAGC 7 cut(s) 114, 477, 1051, 2134, 2229, 2232, 2235
LweI GCATC 7 cut(s) 95, 343, 1208, 1247, 1270, 1872, 1929
MaeI CTAG 5 cut(s) 78, 459, 1590, 1778, 1994
MaeII ACGT 3 cut(s) 700, 739, 855
MaeIII GTNAC 3 cut(s) 1316, 1610, 1780
MfeI CAATTG 1 cut(s) 1034
MflI RGATCY 7 cut(s) 328, 548, 743, 1297, 1487, 1568, 2156
MhlI GDGCHC 8 cut(s) 277, 503, 694, 828, 1014, 1228, 1855, 2075
MlsI TGGCCA 1 cut(s) 948
MluNI TGGCCA 1 cut(s) 948
MlyI GAGTC 5 cut(s) 2, 366, 518, 1157, 1928
MmeI TCCRAC 3 cut(s) 558, 1142, 1414
Mox20I TGGCCA 1 cut(s) 948
Mph1103I ATGCAT 3 cut(s) 110, 1201, 1887
MscI TGGCCA 1 cut(s) 948
MseI TTAA 5 cut(s) 255, 318, 617, 834, 1761
MslI CAYNNNNRTG 7 cut(s) 396, 543, 567, 1218, 1795, 1937, 2266
Msp20I TGGCCA 1 cut(s) 948
MspA1I CMGCKG 2 cut(s) 1277, 1538
MspI CCGG 4 cut(s) 23, 357, 1712, 1727
MspR9I CCNGG 5 cut(s) 553, 603, 1626, 1728, 1875
MunI CAATTG 1 cut(s) 1034
Mva1269I GAATGC 1 cut(s) 964
MvaI CCWGG 4 cut(s) 553, 603, 1626, 1875
MvnI CGCG 1 cut(s) 1697
MwoI GCNNNNNNNGC 6 cut(s) 281, 487, 1436, 1445, 2133, 2223
NciI CCSGG 1 cut(s) 1728
NcoI CCATGG 1 cut(s) 1932
NmuCI GTSAC 2 cut(s) 1316, 1780
NsiI ATGCAT 3 cut(s) 110, 1201, 1887
NspI RCATGY 7 cut(s) 395, 1022, 1421, 1681, 1796, 1889, 1988
PaeI GCATGC 1 cut(s) 1889
PaqCI CACCTGC 1 cut(s) 1250
PciI ACATGT 2 cut(s) 1417, 1792
PctI GAATGC 1 cut(s) 964
PfeI GAWTC 7 cut(s) 117, 724, 758, 1004, 1028, 1531, 1928
PflMI CCANNNNNTGG 1 cut(s) 872
PfoI TCCNGGA 1 cut(s) 551
PkrI GCNGC 9 cut(s) 129, 153, 492, 1041, 2129, 2149, 2219, 2222, 2225
PleI GAGTC 5 cut(s) 2, 366, 517, 1156, 1927
PpsI GAGTC 5 cut(s) 2, 366, 517, 1156, 1927
PpuMI RGGWCCY 2 cut(s) 807, 1286
PscI ACATGT 2 cut(s) 1417, 1792
PshAI GACNNNNGTC 1 cut(s) 182
PsiI TTATAA 2 cut(s) 386, 686
Psp124BI GAGCTC 2 cut(s) 694, 1855
Psp5II RGGWCCY 2 cut(s) 807, 1286
Psp6I CCWGG 4 cut(s) 551, 601, 1624, 1873
PspFI CCCAGC 3 cut(s) 1273, 2206, 2213
PspGI CCWGG 4 cut(s) 551, 601, 1624, 1873
PspPI GGNCC 6 cut(s) 149, 766, 807, 1074, 1286, 1730
PspPPI RGGWCCY 2 cut(s) 807, 1286
PstI CTGCAG 1 cut(s) 1261
PstNI CAGNNNCTG 2 cut(s) 1289, 1625
PsuI RGATCY 7 cut(s) 328, 548, 743, 1297, 1487, 1568, 2156
PvuII CAGCTG 2 cut(s) 1277, 1538
RsaI GTAC 9 cut(s) 5, 190, 207, 338, 1336, 1356, 1363, 1673, 1872
RsaNI GTAC 9 cut(s) 4, 189, 206, 337, 1335, 1355, 1362, 1672, 1871
RseI CAYNNNNRTG 7 cut(s) 396, 543, 567, 1218, 1795, 1937, 2266
SacI GAGCTC 2 cut(s) 694, 1855
SaqAI TTAA 5 cut(s) 255, 318, 617, 834, 1761
SatI GCNGC 9 cut(s) 128, 152, 491, 1040, 2128, 2148, 2218, 2221, 2224
Sau96I GGNCC 6 cut(s) 149, 766, 807, 1074, 1286, 1730
SbfI CCTGCAGG 1 cut(s) 1261
ScaI AGTACT 1 cut(s) 1356
SchI GAGTC 5 cut(s) 2, 366, 518, 1157, 1928
ScrFI CCNGG 5 cut(s) 553, 603, 1626, 1728, 1875
SdaI CCTGCAGG 1 cut(s) 1261
SduI GDGCHC 8 cut(s) 277, 503, 694, 828, 1014, 1228, 1855, 2075
SfaNI GCATC 7 cut(s) 95, 343, 1208, 1247, 1270, 1872, 1929
SfcI CTRYAG 2 cut(s) 1023, 1257
SinI GGWCC 4 cut(s) 766, 807, 1286, 1730
SmiMI CAYNNNNRTG 7 cut(s) 396, 543, 567, 1218, 1795, 1937, 2266
SmlI CTYRAG 3 cut(s) 667, 748, 1110
SmoI CTYRAG 3 cut(s) 667, 748, 1110
SpeI ACTAGT 1 cut(s) 458
SphI GCATGC 1 cut(s) 1889
Sse8387I CCTGCAGG 1 cut(s) 1261
SsiI CCGC 7 cut(s) 34, 96, 152, 1124, 1404, 1695, 2127
SspMI CTAG 5 cut(s) 78, 459, 1590, 1778, 1994
SstI GAGCTC 2 cut(s) 694, 1855
StyD4I CCNGG 5 cut(s) 551, 601, 1624, 1726, 1873
StyI CCWWGG 2 cut(s) 762, 1932
TaaI ACNGT 4 cut(s) 1327, 1553, 1702, 2003
TaiI ACGT 3 cut(s) 703, 742, 858
TaqI TCGA 3 cut(s) 120, 649, 1892
TaqII GACCGA 1 cut(s) 2081
TatI WGTACW 4 cut(s) 188, 205, 1354, 1361
TauI GCSGC 2 cut(s) 154, 2130
TfiI GAWTC 7 cut(s) 117, 724, 758, 1004, 1028, 1531, 1928
Tru1I TTAA 5 cut(s) 255, 318, 617, 834, 1761
Tru9I TTAA 5 cut(s) 255, 318, 617, 834, 1761
TscAI CASTG 9 cut(s) 137, 243, 421, 442, 508, 1330, 1473, 1558, 1564
TseFI GTSAC 2 cut(s) 1316, 1780
TseI GCWGC 7 cut(s) 127, 490, 1039, 2147, 2217, 2220, 2223
Tsp45I GTSAC 2 cut(s) 1316, 1780
TspDTI ATGAA 6 cut(s) 356, 531, 1598, 1779, 1830, 1941
TspGWI ACGGA 5 cut(s) 598, 1112, 1218, 1412, 1740
TspRI CASTG 9 cut(s) 137, 243, 421, 442, 508, 1330, 1473, 1558, 1564
Van91I CCANNNNNTGG 1 cut(s) 872
VneI GTGCAC 1 cut(s) 273
VpaK11BI GGWCC 4 cut(s) 766, 807, 1286, 1730
XapI RAATTY 2 cut(s) 251, 309
XceI RCATGY 7 cut(s) 395, 1022, 1421, 1681, 1796, 1889, 1988
XcmI CCANNNNNNNNNTGG 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 5 cut(s) 78, 459, 1590, 1778, 1994
ZrmI AGTACT 1 cut(s) 1356
Zsp2I ATGCAT 3 cut(s) 110, 1201, 1887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.