pycom08g12460

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0021145
Physical Location & Seq
Forward (+)
10887004 .. 10887700
697 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g12460.1

Sequence Viewer

Length: 426 bp
ATGCATATATTTTCTAACTATGTTGTGAACATGAGGCTCTTATTCATGTCAATAGTGTTAAGCTTATTATGCAGAGAACTAAGGCTTGTTTCCGGTGATATCGGCACAGCAACGTCTTACGGTCCTCCTTACATACCTACAAAGTGCTTTGGAAGTAGGCAAGACCAGTTCCCTCCGGGGAATCTCTTTGTGGCGGTGAGTGAAGGGTTGTGGGACAATGGTGCTGCGTGCGGAAGGCGGTATAGACTGAGGTGCCTGAGTGGACGTAATAAGCCCTGCAAGGGTGGTGCTACTGTGGACGTGAAGGTGGTTGATCTTTGTCGAGATTCAACCTGCCCTTCTACCGTAGCCATGTCAACTGATGCTTTTGCAGCCATCTCACACTCTCCTTCTACAAGAATCAACGTCGAATATGTCCAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

142

Amino Acids

15.43

Weight (kDa)

8.94

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 65 - 138 3.7e-10 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021145)

Species Orthologous Gene IDs
malus_domestica MD08G1147800.v1.1 MD15G1123700.v1.1
prunus_persica Prupe.1G479000_v2.0.a1
pyrus_communis pycom08g12460 pycom15g11140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 252
AciI CCGC 3 cut(s) 194, 231, 238
AfiI CCNNNNNNNGG 1 cut(s) 281
AgsI TTSAA 1 cut(s) 330
AjiI CACGTC 1 cut(s) 301
AluBI AGCT 1 cut(s) 63
AluI AGCT 1 cut(s) 63
ApeKI GCWGC 2 cut(s) 224, 371
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 1 cut(s) 177
AsuHPI GGTGA 2 cut(s) 107, 208
AvaII GGWCC 1 cut(s) 122
BanI GGYRCC 1 cut(s) 252
BbvI GCAGC 2 cut(s) 211, 383
BccI CCATC 1 cut(s) 383
BcnI CCSGG 1 cut(s) 177
BfuAI ACCTGC 1 cut(s) 341
BisI GCNGC 2 cut(s) 225, 372
BlsI GCNGC 2 cut(s) 226, 373
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 1 cut(s) 122
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 254
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 352
BpuMI CCSGG 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 176
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 166
BseDI CCNNGG 1 cut(s) 176
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMII CTCAG 2 cut(s) 239, 248
BseNI ACTGG 1 cut(s) 166
BseXI GCAGC 2 cut(s) 211, 383
BshNI GGYRCC 1 cut(s) 252
BsiSI CCGG 2 cut(s) 93, 176
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 227
Bsp143I GATC 1 cut(s) 313
BspACI CCGC 3 cut(s) 194, 231, 238
BspCNI CTCAG 2 cut(s) 240, 249
BspLI GGNNCC 1 cut(s) 254
BspMI ACCTGC 1 cut(s) 341
BspT107I GGYRCC 1 cut(s) 252
BsrI ACTGG 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 1 cut(s) 313
Bst4CI ACNGT 3 cut(s) 122, 295, 346
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 3 cut(s) 80, 248, 257
BstKTI GATC 1 cut(s) 316
BstMBI GATC 1 cut(s) 313
BstMWI GCNNNNNNNGC 2 cut(s) 69, 371
BstSCI CCNGG 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 211, 383
BtrI CACGTC 1 cut(s) 301
BveI ACCTGC 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 122
CviAII CATG 3 cut(s) 31, 46, 352
CviJI RGCY 6 cut(s) 37, 63, 85, 274, 350, 374
CviKI_1 RGCY 6 cut(s) 37, 63, 85, 274, 350, 374
DdeI CTNAG 3 cut(s) 80, 248, 257
DpnI GATC 1 cut(s) 315
DpnII GATC 1 cut(s) 313
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 122
EcoRV GATATC 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 34, 49, 355
FaqI GGGAC 1 cut(s) 227
FatI CATG 3 cut(s) 30, 45, 351
Fnu4HI GCNGC 2 cut(s) 225, 372
Fsp4HI GCNGC 2 cut(s) 225, 372
GluI GCNGC 2 cut(s) 225, 372
HapII CCGG 2 cut(s) 93, 176
Hin1II CATG 3 cut(s) 34, 49, 355
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 3 cut(s) 181, 326, 399
HpaII CCGG 2 cut(s) 93, 176
HphI GGTGA 2 cut(s) 107, 208
Hpy166II GTNNAC 4 cut(s) 28, 263, 298, 357
Hpy188III TCNNGA 2 cut(s) 323, 418
Hpy8I GTNNAC 4 cut(s) 28, 263, 298, 357
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 5 cut(s) 197, 228, 298, 348, 399
HpyCH4III ACNGT 3 cut(s) 122, 295, 346
HpyCH4IV ACGT 4 cut(s) 113, 265, 300, 405
HpyCH4V TGCA 4 cut(s) 4, 72, 279, 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 371
HpyF3I CTNAG 3 cut(s) 80, 248, 257
HpySE526I ACGT 4 cut(s) 113, 265, 300, 405
Hsp92II CATG 3 cut(s) 34, 49, 355
Kzo9I GATC 1 cut(s) 313
LpnPI CCDG 6 cut(s) 106, 179, 189, 269, 289, 346
Lsp1109I GCAGC 2 cut(s) 211, 383
LweI GCATC 1 cut(s) 352
MaeII ACGT 4 cut(s) 113, 265, 300, 405
MalI GATC 1 cut(s) 315
MboI GATC 1 cut(s) 313
MnlI CCTC 4 cut(s) 27, 135, 183, 243
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 59
MspI CCGG 2 cut(s) 93, 176
MspR9I CCNGG 1 cut(s) 177
MwoI GCNNNNNNNGC 2 cut(s) 69, 371
NciI CCSGG 1 cut(s) 177
NdeII GATC 1 cut(s) 313
NlaIII CATG 3 cut(s) 34, 49, 355
NlaIV GGNNCC 1 cut(s) 254
NsiI ATGCAT 1 cut(s) 6
PfeI GAWTC 3 cut(s) 181, 326, 399
PkrI GCNGC 2 cut(s) 226, 373
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 59
SatI GCNGC 2 cut(s) 225, 372
Sau3AI GATC 1 cut(s) 313
Sau96I GGNCC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 177
SetI ASST 9 cut(s) 65, 116, 139, 254, 268, 303, 309, 335, 408
SfaNI GCATC 1 cut(s) 352
SinI GGWCC 1 cut(s) 122
SsiI CCGC 3 cut(s) 194, 231, 238
StyD4I CCNGG 1 cut(s) 175
TaaI ACNGT 3 cut(s) 122, 295, 346
TaiI ACGT 4 cut(s) 116, 268, 303, 408
TaqI TCGA 2 cut(s) 322, 408
TfiI GAWTC 3 cut(s) 181, 326, 399
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TseI GCWGC 2 cut(s) 224, 371
TspDTI ATGAA 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 122
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.