pycom08g15830

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0016156
Physical Location & Seq
Reverse (-)
15613545 .. 15614771
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g15830.1

Sequence Viewer

Length: 1227 bp
ATGGCGTTTTGGCGCCTCCTCATTTCCAAAACCCTAAACCCTAAACCCTCACCTCCCCTCCCAAACACTAACCACTCGCCGCCCTCCCTCCTCCCTCTCCTCCTCCGTCAAACCTCACCGTTCTCCACCTCCTTTCTCATCACCAAAACCCCAAAAAAATTCAGAAAAAAGCGCAAGAAGGACGAAAGCCCCCGAACCAAGCTCGTCCAGACTCAGCCCAACCTCATCCCCCACTTCGAACACATTGTCGAGCGCGACGCTTACTTCCGATTCCTCACCAAATCGAAAGACTTCCTCTCCAAGCAGCCCCACCGCGTCCTCCGCCTCGATGATGCCGGCAAGCTCTACCGCGAACTCGGCTTCCCTCGCGGCCGAAAAGTCGCCCGATCCCTCCAGCGCCACCCCCTCATCTTCCAAACCTACCGCCACGATGAGAACAAAATGTGGATCGGATTCACCGACCTCATGGAAGCTCTGCTCGAAGAGGAGCGAGTCATAATGGACTCAATGGAATCGGACCGAGTCAACAAGGTTCGTAAATTGCTCATGATATCCACCAACAAGCGTATCCCTTTGAGTAAAATCTACCATTGCAGGTCGATTTTCGGAATTCCTGAAGATTTTAGGGACAGGGTTTCGAAATACCCTAATTTTTTCAGGATTGTGGTTGAGGAGGATGGGAAGAGAGTGCTTGAAGTGGTGAACTGGGATCCGTTGATGGCGGTGAGCGCATTGGAGAGGGAGTTTATGGTGGATGAGGAGAGAGTGAAGAGGGCTTTTCGGTTTCCAGTGAAGCATGGGAAGGATTTGGGCCTGGAGATAGAGGATGAGAGGAAATTGAACATGTTGAATTCGCTTCCGTTGGTTTCGCCTTATTCGGATGGATCAAGATTGGACCTTTGGACTCTGGAAGCGGAGAAGTACAGGTTGGGAGTGTTGCATGAGTTTTTGAGCTTGACATTGGAGAAGAGGGCTTCCATACACCATGTTGTGGAGTTCAAGGAGGAGTTTAGCTTGAGCAAGCATACTTATCAGATGCTGTTGAAGCAGCCCAGAAAGTTTTATCTGGCCGGGACGGAGATGAACTGGGTTGTTTTCTTGAAAGATGCTTATGACCGAGACAGGGTTTTGATTGAGAAAGATCCGCAGGTGGTTTTCAATGAGAAGTTGTACAAGTATGCTGATATGAAGGAAAGGGAACAAGGATTTGTTGATCAATGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

409

Amino Acids

48.6

Weight (kDa)

9.65

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 77 - 398 1.4e-98 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08940
fragaria_vesca FvH4_2g09730 FvH4_2g09730
malus_domestica MD00G1009900.v1.1
prunus_persica Prupe.1G518700_v2.0.a1
pyrus_communis pycom08g15830 pycom15g33270
rosa_chinensis RchiOBHm_Chr7g0238641
rosa_laevigata RLG00000000822
rosa_multiflora Rmu_sc0000023.1_g000009 Rmu_sc0030975.1_g000001
rosa_roxburghii Rroxscaffold_3G00221660
rosa_rugosa Rorug07G0322500
rosa_samantha Rh7BG448100
rosa_wichuraiana Rw7G039810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1138
Acc36I ACCTGC 2 cut(s) 585, 1138
AccB1I GGYRCC 1 cut(s) 12
AccB7I CCANNNNNTGG 1 cut(s) 991
AccII CGCG 4 cut(s) 255, 315, 351, 369
AciI CCGC 9 cut(s) 80, 313, 322, 349, 369, 424, 722, 914, 1145
AclWI GGATC 6 cut(s) 381, 455, 704, 717, 892, 1136
AcoI YGGCCR 2 cut(s) 370, 1068
AcsI RAATTY 3 cut(s) 158, 609, 850
AcuI CTGAAG 1 cut(s) 636
AcyI GRCGYC 1 cut(s) 13
AfaI GTAC 2 cut(s) 923, 1172
AfiI CCNNNNNNNGG 2 cut(s) 991, 1123
AflIII ACRYGT 1 cut(s) 843
AgsI TTSAA 7 cut(s) 695, 841, 850, 1000, 1045, 1102, 1159
AjnI CCWGG 1 cut(s) 813
AjuI GAANNNNNNNTTGG 2 cut(s) 911, 943
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 5 cut(s) 202, 343, 473, 954, 1014
AluI AGCT 5 cut(s) 202, 343, 473, 954, 1014
Alw26I GTCTC 1 cut(s) 1113
AlwI GGATC 6 cut(s) 381, 455, 704, 717, 892, 1136
AlwNI CAGNNNCTG 1 cut(s) 1039
AoxI GGCC 3 cut(s) 370, 811, 1068
ApeKI GCWGC 2 cut(s) 304, 1048
ApoI RAATTY 3 cut(s) 158, 609, 850
ArsI GACNNNNNNTTYG 2 cut(s) 231, 263
Asp700I GAANNNNTTC 1 cut(s) 290
AspLEI GCGC 5 cut(s) 15, 174, 255, 399, 731
AspS9I GGNCC 3 cut(s) 517, 811, 895
AsuC2I CCSGG 1 cut(s) 1072
AsuHPI GGTGA 7 cut(s) 42, 108, 133, 268, 448, 712, 736
AsuII TTCGAA 2 cut(s) 237, 638
AvaII GGWCC 2 cut(s) 517, 895
BaeI ACNNNNGTAYC 2 cut(s) 550, 583
BamHI GGATCC 1 cut(s) 709
BanI GGYRCC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 316, 1060
BccI CCATC 3 cut(s) 671, 712, 875
BciT130I CCWGG 1 cut(s) 815
BciVI GTATCC 1 cut(s) 578
BclI TGATCA 1 cut(s) 1213
BcnI CCSGG 1 cut(s) 1072
BcoDI GTCTC 1 cut(s) 1113
BfoI RGCGCY 2 cut(s) 16, 400
BfuAI ACCTGC 2 cut(s) 585, 1138
BfuI GTATCC 1 cut(s) 578
BisI GCNGC 4 cut(s) 80, 305, 370, 1049
BlsI GCNGC 4 cut(s) 81, 306, 371, 1050
Bme1390I CCNGG 2 cut(s) 815, 1072
Bme18I GGWCC 2 cut(s) 517, 895
BmgT120I GGNCC 3 cut(s) 517, 811, 895
BmiI GGNNCC 2 cut(s) 14, 711
BmrFI CCNGG 2 cut(s) 815, 1072
BmrI ACTGGG 2 cut(s) 715, 1096
BmsI GCATC 3 cut(s) 322, 1026, 1096
BmuI ACTGGG 2 cut(s) 715, 1096
BpmI CTGGAG 2 cut(s) 377, 836
Bpu14I TTCGAA 2 cut(s) 237, 638
BpuEI CTTGAG 1 cut(s) 1036
BpuMI CCSGG 1 cut(s) 1072
BsaHI GRCGYC 1 cut(s) 13
BsaXI ACNNNNNCTCC 6 cut(s) 42, 72, 281, 311, 728, 758
Bsc4I CCNNNNNNNGG 2 cut(s) 991, 1123
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 3 cut(s) 710, 788, 1091
Bse3DI GCAATG 1 cut(s) 589
BseBI CCWGG 1 cut(s) 815
BseGI GGATG 5 cut(s) 225, 682, 760, 832, 886
BseLI CCNNNNNNNGG 2 cut(s) 991, 1123
BseMI GCAATG 1 cut(s) 589
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 3 cut(s) 710, 788, 1091
BseRI GAGGAG 8 cut(s) 8, 80, 89, 92, 500, 686, 773, 1019
BseX3I CGGCCG 1 cut(s) 370
BseXI GCAGC 2 cut(s) 316, 1060
Bsh1236I CGCG 4 cut(s) 255, 315, 351, 369
Bsh1285I CGRYCG 1 cut(s) 373
BshFI GGCC 3 cut(s) 372, 813, 1070
BshNI GGYRCC 1 cut(s) 12
BsiEI CGRYCG 1 cut(s) 373
BsiSI CCGG 2 cut(s) 336, 1071
BslFI GGGAC 2 cut(s) 641, 1087
BslI CCNNNNNNNGG 2 cut(s) 991, 1123
BsmAI GTCTC 1 cut(s) 1113
BsmFI GGGAC 2 cut(s) 641, 1087
BsnI GGCC 3 cut(s) 372, 813, 1070
Bsp119I TTCGAA 2 cut(s) 237, 638
Bsp1407I TGTACA 1 cut(s) 1170
Bsp143I GATC 6 cut(s) 386, 447, 709, 884, 1141, 1213
BspACI CCGC 9 cut(s) 80, 313, 322, 349, 369, 424, 722, 914, 1145
BspANI GGCC 3 cut(s) 372, 813, 1070
BspCNI CTCAG 1 cut(s) 226
BspFNI CGCG 4 cut(s) 255, 315, 351, 369
BspHI TCATGA 1 cut(s) 546
BspLI GGNNCC 2 cut(s) 14, 711
BspMI ACCTGC 2 cut(s) 585, 1138
BspPI GGATC 6 cut(s) 381, 455, 704, 717, 892, 1136
BspT104I TTCGAA 2 cut(s) 237, 638
BspT107I GGYRCC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 589
BsrFI RCCGGY 1 cut(s) 335
BsrGI TGTACA 1 cut(s) 1170
BsrI ACTGG 3 cut(s) 710, 788, 1091
BssAI RCCGGY 1 cut(s) 335
BssMI GATC 6 cut(s) 386, 447, 709, 884, 1141, 1213
BssNI GRCGYC 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 815
Bst4CI ACNGT 1 cut(s) 120
Bst6I CTCTTC 4 cut(s) 477, 677, 764, 962
BstACI GRCGYC 1 cut(s) 13
BstAUI TGTACA 1 cut(s) 1170
BstBI TTCGAA 2 cut(s) 237, 638
BstC8I GCNNGC 3 cut(s) 337, 341, 1022
BstDEI CTNAG 1 cut(s) 213
BstF5I GGATG 5 cut(s) 225, 682, 760, 832, 886
BstFNI CGCG 4 cut(s) 255, 315, 351, 369
BstH2I RGCGCY 2 cut(s) 16, 400
BstHHI GCGC 5 cut(s) 15, 174, 255, 399, 731
BstKTI GATC 6 cut(s) 389, 450, 712, 887, 1144, 1216
BstMAI GTCTC 1 cut(s) 1113
BstMBI GATC 6 cut(s) 386, 447, 709, 884, 1141, 1213
BstMCI CGRYCG 1 cut(s) 373
BstMWI GCNNNNNNNGC 5 cut(s) 321, 357, 366, 728, 1045
BstNI CCWGG 1 cut(s) 815
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 2 cut(s) 813, 1070
BstUI CGCG 4 cut(s) 255, 315, 351, 369
BstV1I GCAGC 2 cut(s) 316, 1060
BstX2I RGATCY 2 cut(s) 709, 1141
BstYI RGATCY 2 cut(s) 709, 1141
BstZI CGGCCG 1 cut(s) 370
BsuI GTATCC 1 cut(s) 578
BsuRI GGCC 3 cut(s) 372, 813, 1070
BtsCI GGATG 5 cut(s) 225, 682, 760, 832, 886
BtsIMutI CAGTG 1 cut(s) 795
BveI ACCTGC 2 cut(s) 585, 1138
Cac8I GCNNGC 3 cut(s) 337, 341, 1022
CaiI CAGNNNCTG 1 cut(s) 1039
CciI TCATGA 1 cut(s) 546
CfoI GCGC 5 cut(s) 15, 174, 255, 399, 731
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 3 cut(s) 517, 811, 895
CpoI CGGWCCG 1 cut(s) 517
CseI GACGC 2 cut(s) 266, 304
Csp6I GTAC 2 cut(s) 922, 1171
CspI CGGWCCG 1 cut(s) 517
CviAII CATG 6 cut(s) 466, 547, 797, 844, 941, 986
CviQI GTAC 2 cut(s) 922, 1171
DdeI CTNAG 1 cut(s) 213
DinI GGCGCC 1 cut(s) 14
DpnI GATC 6 cut(s) 388, 449, 711, 886, 1143, 1215
DpnII GATC 6 cut(s) 386, 447, 709, 884, 1141, 1213
EaeI YGGCCR 2 cut(s) 370, 1068
EagI CGGCCG 1 cut(s) 370
Eam1104I CTCTTC 4 cut(s) 477, 677, 764, 962
EarI CTCTTC 4 cut(s) 477, 677, 764, 962
EciI GGCGGA 1 cut(s) 311
EclXI CGGCCG 1 cut(s) 370
Eco32I GATATC 1 cut(s) 552
Eco47I GGWCC 2 cut(s) 517, 895
Eco52I CGGCCG 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 636
EcoRI GAATTC 2 cut(s) 609, 850
EcoRII CCWGG 1 cut(s) 813
EcoRV GATATC 1 cut(s) 552
EgeI GGCGCC 1 cut(s) 14
EheI GGCGCC 1 cut(s) 14
FaeI CATG 6 cut(s) 469, 550, 800, 847, 944, 989
FaqI GGGAC 2 cut(s) 641, 1087
FatI CATG 6 cut(s) 465, 546, 796, 843, 940, 985
FbaI TGATCA 1 cut(s) 1213
Fnu4HI GCNGC 4 cut(s) 80, 305, 370, 1049
FokI GGATG 5 cut(s) 212, 689, 767, 839, 893
Fsp4HI GCNGC 4 cut(s) 80, 305, 370, 1049
GlaI GCGC 5 cut(s) 14, 173, 254, 398, 730
GluI GCNGC 4 cut(s) 80, 305, 370, 1049
GsuI CTGGAG 2 cut(s) 377, 836
HaeII RGCGCY 2 cut(s) 16, 400
HaeIII GGCC 3 cut(s) 372, 813, 1070
HapII CCGG 2 cut(s) 336, 1071
HgaI GACGC 2 cut(s) 266, 304
HhaI GCGC 5 cut(s) 15, 174, 255, 399, 731
Hin1I GRCGYC 1 cut(s) 13
Hin1II CATG 6 cut(s) 469, 550, 800, 847, 944, 989
Hin6I GCGC 5 cut(s) 13, 172, 253, 397, 729
HinP1I GCGC 5 cut(s) 13, 172, 253, 397, 729
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 8 cut(s) 211, 270, 453, 492, 503, 512, 522, 904
HpaII CCGG 2 cut(s) 336, 1071
HphI GGTGA 7 cut(s) 42, 108, 133, 268, 448, 712, 736
Hpy166II GTNNAC 2 cut(s) 526, 703
Hpy188I TCNGA 7 cut(s) 164, 269, 452, 517, 608, 880, 1035
Hpy188III TCNNGA 7 cut(s) 208, 547, 614, 658, 888, 908, 1099
Hpy8I GTNNAC 2 cut(s) 526, 703
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 172, 796, 1183
HpyCH4III ACNGT 1 cut(s) 120
HpyCH4V TGCA 2 cut(s) 594, 940
HpyF10VI GCNNNNNNNGC 5 cut(s) 321, 357, 366, 728, 1045
HpyF3I CTNAG 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 13
Hsp92II CATG 6 cut(s) 469, 550, 800, 847, 944, 989
HspAI GCGC 5 cut(s) 13, 172, 253, 397, 729
KasI GGCGCC 1 cut(s) 12
KroI GCCGGC 1 cut(s) 335
KroNI GCCGGC 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 1213
Kzo9I GATC 6 cut(s) 386, 447, 709, 884, 1141, 1213
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 2 cut(s) 316, 1060
LweI GCATC 3 cut(s) 322, 1026, 1096
MalI GATC 6 cut(s) 388, 449, 711, 886, 1143, 1215
MboI GATC 6 cut(s) 386, 447, 709, 884, 1141, 1213
MboII GAAGA 6 cut(s) 403, 494, 629, 694, 781, 979
MflI RGATCY 2 cut(s) 709, 1141
MluCI AATT 6 cut(s) 158, 539, 609, 649, 836, 850
Mly113I GGCGCC 1 cut(s) 13
MlyI GAGTC 5 cut(s) 205, 497, 501, 531, 898
MroNI GCCGGC 1 cut(s) 335
MroXI GAANNNNTTC 1 cut(s) 290
MspI CCGG 2 cut(s) 336, 1071
MspR9I CCNGG 2 cut(s) 815, 1072
MvaI CCWGG 1 cut(s) 815
MvnI CGCG 4 cut(s) 255, 315, 351, 369
MwoI GCNNNNNNNGC 5 cut(s) 321, 357, 366, 728, 1045
NaeI GCCGGC 1 cut(s) 337
NarI GGCGCC 1 cut(s) 13
NciI CCSGG 1 cut(s) 1072
NdeII GATC 6 cut(s) 386, 447, 709, 884, 1141, 1213
NgoMIV GCCGGC 1 cut(s) 335
NlaIII CATG 6 cut(s) 469, 550, 800, 847, 944, 989
NlaIV GGNNCC 2 cut(s) 14, 711
NmeAIII GCCGAG 1 cut(s) 336
NspI RCATGY 1 cut(s) 847
NspV TTCGAA 2 cut(s) 237, 638
PagI TCATGA 1 cut(s) 546
PaqCI CACCTGC 1 cut(s) 1138
PciI ACATGT 1 cut(s) 843
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PdiI GCCGGC 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 3 cut(s) 270, 453, 512
PflFI GACNNNGTC 1 cut(s) 521
PflMI CCANNNNNTGG 1 cut(s) 991
PkrI GCNGC 4 cut(s) 81, 306, 371, 1050
PleI GAGTC 5 cut(s) 205, 497, 500, 530, 898
PluTI GGCGCC 1 cut(s) 16
PpsI GAGTC 5 cut(s) 205, 497, 500, 530, 898
PscI ACATGT 1 cut(s) 843
Psp6I CCWGG 1 cut(s) 813
PspGI CCWGG 1 cut(s) 813
PspN4I GGNNCC 2 cut(s) 14, 711
PspPI GGNCC 3 cut(s) 517, 811, 895
PstNI CAGNNNCTG 1 cut(s) 1039
PsuI RGATCY 2 cut(s) 709, 1141
PsyI GACNNNGTC 1 cut(s) 521
RsaI GTAC 2 cut(s) 923, 1172
RsaNI GTAC 2 cut(s) 922, 1171
Rsr2I CGGWCCG 1 cut(s) 517
RsrII CGGWCCG 1 cut(s) 517
SatI GCNGC 4 cut(s) 80, 305, 370, 1049
Sau3AI GATC 6 cut(s) 386, 447, 709, 884, 1141, 1213
Sau96I GGNCC 3 cut(s) 517, 811, 895
SchI GAGTC 5 cut(s) 205, 497, 501, 531, 898
ScrFI CCNGG 2 cut(s) 815, 1072
SfaNI GCATC 3 cut(s) 322, 1026, 1096
SfoI GGCGCC 1 cut(s) 14
SfuI TTCGAA 2 cut(s) 237, 638
SinI GGWCC 2 cut(s) 517, 895
SmlI CTYRAG 1 cut(s) 1015
SmoI CTYRAG 1 cut(s) 1015
Sse9I AATT 6 cut(s) 158, 539, 609, 649, 836, 850
SsiI CCGC 9 cut(s) 80, 313, 322, 349, 369, 424, 722, 914, 1145
SspDI GGCGCC 1 cut(s) 12
StyD4I CCNGG 2 cut(s) 813, 1070
TaaI ACNGT 1 cut(s) 120
TaqI TCGA 7 cut(s) 237, 249, 284, 327, 480, 599, 638
TaqII GACCGA 2 cut(s) 534, 1131
TasI AATT 6 cut(s) 158, 539, 609, 649, 836, 850
TatI WGTACW 2 cut(s) 921, 1170
TauI GCSGC 2 cut(s) 82, 372
TfiI GAWTC 3 cut(s) 270, 453, 512
TscAI CASTG 1 cut(s) 795
TseI GCWGC 2 cut(s) 304, 1048
TspDTI ATGAA 2 cut(s) 1097, 1202
TspGWI ACGGA 4 cut(s) 95, 702, 849, 1091
TspRI CASTG 1 cut(s) 795
Tth111I GACNNNGTC 1 cut(s) 521
Van91I CCANNNNNTGG 1 cut(s) 991
VpaK11BI GGWCC 2 cut(s) 517, 895
XapI RAATTY 3 cut(s) 158, 609, 850
XceI RCATGY 1 cut(s) 847
XmnI GAANNNNTTC 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.