pycom15g33270

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016156
Physical Location & Seq
Reverse (-)
32572852 .. 32574069
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33270.1

Sequence Viewer

Length: 1218 bp
ATGGCGTTTTGGCGCCTCATCCTTTCCAAAACCCCAAACCCTAAACCCTCACCTCACCTCCCAAACCTTAACCACTCGCAGCCCTCACTCCTCCTCCACCAAACCTCGCCGTTCTCCACCTCCTTCCTCATCACCAAAACCCCCAAAAAATTCAGAAAAAAACGCAAGAAGGACGAAAGCCCTCGGACCAAGCTCGTCCAGACCCAACCCAACCTCATCCCCCACTTCGAACACATCGTCGAGCGCGACGCCTACTTTCGATTCCTCACCAAATCCAAAGATTTCCTCTCCAAGCAGCCCCACCGCGTCCTCCGCCTCGACGACGCCGGCAAGCTCTACCGCGAACTCGGCTTCCCTCGCGGCCGAAAAGTCGCCCGCTCCCTCCAGCGCCACCCCCTAATCTTCCAGACCTACCGCCACGATGACAATAAAATGTGGATCGGCTTCACCGACTTCATGGAAGCTCTGCTCGAAGAGGAGCGAGTCATAATCGACTCAATGGAGTTGGACCGAGTCAACAAGGTTCGTAAATTGCTCATGATGTCCGCCAACAAGCGTATCCCTCTGAGTAAAATCTACCATTGCAGGTCGATTTTTGGAATTCCTGAAGATTTTAGGGACAGGGTTTCGAAATACCCTAATTTTTTCAGGATTGTGGTTGAGGAGGATGGGAAGAGAGTGCTTGAACTGGTGAATTGGGATCCGTTGATGGCGGTGAGCGCGATGGAGAGGGAGTTCATGGTGGATGAGGAGAGAGTGAAGAGGGCTTTTCGGTTTCCAGTGAAGCATGGGAAGGATTTGAGTCTGGAGATAGAGGATGAGAGGAAATTGAACATGTTGAACTCACTTCCGCTGGTTTTGCCATATTCGGTTCTAGATCGATCAAGATTGGACCTTTGGACTCTGGAAGCCGAGAAGTACAGGGTAGGAGTGTTGCATGAGTTTTTGAGCTTGACATTGGAGAAGAGGGCTTCCATACACCATGTTGTGGAGTTCAAGGAGGAGTTTAGCTTGACTAAGCATACTTATCAGATGCTTTTGAAGCAGCCCAGAACGTTTTATCTTGCTGGGACGGAGATGAATTGGGTTGTTTTTCTGAAATATGCTTATGACCTAGATGGGGTTTTGATTGAGAAGGATCCCCAGGTGGTGTTCAATGAGAAGTTGTATAAGCATGCTGATATGAAGGAACTGGGACAGGGCTGTGTTGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

406

Amino Acids

48.09

Weight (kDa)

9.49

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 74 - 395 6.4e-96 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08940
fragaria_vesca FvH4_2g09730 FvH4_2g09730
malus_domestica MD00G1009900.v1.1
prunus_persica Prupe.1G518700_v2.0.a1
pyrus_communis pycom08g15830 pycom15g33270
rosa_chinensis RchiOBHm_Chr7g0238641
rosa_laevigata RLG00000000822
rosa_multiflora Rmu_sc0000023.1_g000009 Rmu_sc0030975.1_g000001
rosa_roxburghii Rroxscaffold_3G00221660
rosa_rugosa Rorug07G0322500
rosa_samantha Rh7BG448100
rosa_wichuraiana Rw7G039810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 576
AccB1I GGYRCC 1 cut(s) 12
AccB7I CCANNNNNTGG 1 cut(s) 988
AccBSI CCGCTC 1 cut(s) 378
AccII CGCG 5 cut(s) 246, 306, 342, 360, 722
AciI CCGC 9 cut(s) 304, 313, 340, 360, 376, 415, 546, 713, 851
AclI AACGTT 1 cut(s) 1055
AclWI GGATC 5 cut(s) 446, 695, 708, 1133, 1146
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 2 cut(s) 149, 600
AcuI CTGAAG 1 cut(s) 627
AcyI GRCGYC 3 cut(s) 13, 249, 324
AfaI GTAC 1 cut(s) 920
AfiI CCNNNNNNNGG 2 cut(s) 988, 1120
AflIII ACRYGT 1 cut(s) 834
AgsI TTSAA 6 cut(s) 686, 832, 841, 997, 1042, 1156
AjnI CCWGG 1 cut(s) 1143
AloI GAACNNNNNNTCC 4 cut(s) 336, 368, 719, 751
AluBI AGCT 5 cut(s) 193, 334, 464, 951, 1011
AluI AGCT 5 cut(s) 193, 334, 464, 951, 1011
AlwI GGATC 5 cut(s) 446, 695, 708, 1133, 1146
AoxI GGCC 1 cut(s) 361
ApeKI GCWGC 3 cut(s) 79, 295, 1045
ApoI RAATTY 2 cut(s) 149, 600
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AspLEI GCGC 4 cut(s) 15, 246, 390, 722
AspS9I GGNCC 3 cut(s) 186, 508, 892
AsuHPI GGTGA 7 cut(s) 42, 47, 124, 259, 439, 703, 727
AsuII TTCGAA 2 cut(s) 228, 629
AvaII GGWCC 3 cut(s) 186, 508, 892
BaeI ACNNNNGTAYC 2 cut(s) 541, 574
BamHI GGATCC 2 cut(s) 700, 1138
BanI GGYRCC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 91, 307, 1057
BccI CCATC 4 cut(s) 662, 703, 718, 1112
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 1 cut(s) 1145
BciVI GTATCC 1 cut(s) 569
BclI TGATCA 1 cut(s) 1210
BfaI CTAG 2 cut(s) 875, 1115
BfoI RGCGCY 2 cut(s) 16, 391
BfuAI ACCTGC 1 cut(s) 576
BfuI GTATCC 1 cut(s) 569
BisI GCNGC 4 cut(s) 80, 296, 361, 1046
BlsI GCNGC 4 cut(s) 81, 297, 362, 1047
Bme1390I CCNGG 1 cut(s) 1145
Bme18I GGWCC 3 cut(s) 186, 508, 892
BmgT120I GGNCC 3 cut(s) 186, 508, 892
BmiI GGNNCC 3 cut(s) 14, 702, 1140
BmrFI CCNGG 1 cut(s) 1145
BmrI ACTGGG 1 cut(s) 1202
BmsI GCATC 1 cut(s) 1023
BmuI ACTGGG 1 cut(s) 1202
BpmI CTGGAG 2 cut(s) 368, 827
Bpu14I TTCGAA 2 cut(s) 228, 629
Bsa29I ATCGAT 1 cut(s) 880
BsaHI GRCGYC 3 cut(s) 13, 249, 324
BsaJI CCNNGG 2 cut(s) 182, 1143
BsaXI ACNNNNNCTCC 6 cut(s) 42, 72, 78, 108, 719, 749
Bsc4I CCNNNNNNNGG 2 cut(s) 988, 1120
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 3 cut(s) 693, 779, 1197
Bse3DI GCAATG 1 cut(s) 580
BseBI CCWGG 1 cut(s) 1145
BseCI ATCGAT 1 cut(s) 880
BseDI CCNNGG 2 cut(s) 182, 1143
BseGI GGATG 5 cut(s) 18, 216, 673, 751, 823
BseLI CCNNNNNNNGG 2 cut(s) 988, 1120
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 1 cut(s) 557
BseNI ACTGG 3 cut(s) 693, 779, 1197
BseRI GAGGAG 6 cut(s) 80, 83, 491, 677, 764, 1016
BseX3I CGGCCG 1 cut(s) 361
BseXI GCAGC 3 cut(s) 91, 307, 1057
BseYI CCCAGC 1 cut(s) 1067
Bsh1236I CGCG 5 cut(s) 246, 306, 342, 360, 722
Bsh1285I CGRYCG 1 cut(s) 364
BshFI GGCC 1 cut(s) 363
BshNI GGYRCC 1 cut(s) 12
BshVI ATCGAT 1 cut(s) 880
BsiEI CGRYCG 1 cut(s) 364
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 3 cut(s) 632, 1084, 1209
BslI CCNNNNNNNGG 2 cut(s) 988, 1120
BsmFI GGGAC 3 cut(s) 632, 1084, 1209
BsnI GGCC 1 cut(s) 363
Bsp119I TTCGAA 2 cut(s) 228, 629
Bsp143I GATC 6 cut(s) 438, 700, 877, 881, 1138, 1210
BspACI CCGC 9 cut(s) 304, 313, 340, 360, 376, 415, 546, 713, 851
BspANI GGCC 1 cut(s) 363
BspCNI CTCAG 1 cut(s) 558
BspDI ATCGAT 1 cut(s) 880
BspFNI CGCG 5 cut(s) 246, 306, 342, 360, 722
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 3 cut(s) 14, 702, 1140
BspMI ACCTGC 1 cut(s) 576
BspPI GGATC 5 cut(s) 446, 695, 708, 1133, 1146
BspT104I TTCGAA 2 cut(s) 228, 629
BspT107I GGYRCC 1 cut(s) 12
BsrBI CCGCTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 3 cut(s) 693, 779, 1197
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 2 cut(s) 182, 1143
BssMI GATC 6 cut(s) 438, 700, 877, 881, 1138, 1210
BssNI GRCGYC 3 cut(s) 13, 249, 324
Bst2UI CCWGG 1 cut(s) 1145
Bst6I CTCTTC 4 cut(s) 468, 668, 755, 959
BstACI GRCGYC 3 cut(s) 13, 249, 324
BstBI TTCGAA 2 cut(s) 228, 629
BstC8I GCNNGC 4 cut(s) 328, 332, 376, 1176
BstDEI CTNAG 2 cut(s) 566, 1017
BstF5I GGATG 5 cut(s) 18, 216, 673, 751, 823
BstFNI CGCG 5 cut(s) 246, 306, 342, 360, 722
BstH2I RGCGCY 2 cut(s) 16, 391
BstHHI GCGC 4 cut(s) 15, 246, 390, 722
BstKTI GATC 6 cut(s) 441, 703, 880, 884, 1141, 1213
BstMBI GATC 6 cut(s) 438, 700, 877, 881, 1138, 1210
BstMCI CGRYCG 1 cut(s) 364
BstMWI GCNNNNNNNGC 6 cut(s) 312, 348, 357, 719, 859, 1042
BstNI CCWGG 1 cut(s) 1145
BstNSI RCATGY 2 cut(s) 838, 1178
BstSCI CCNGG 1 cut(s) 1143
BstUI CGCG 5 cut(s) 246, 306, 342, 360, 722
BstV1I GCAGC 3 cut(s) 91, 307, 1057
BstX2I RGATCY 2 cut(s) 700, 1138
BstYI RGATCY 2 cut(s) 700, 1138
BstZI CGGCCG 1 cut(s) 361
Bsu15I ATCGAT 1 cut(s) 880
BsuI GTATCC 1 cut(s) 569
BsuRI GGCC 1 cut(s) 363
BsuTUI ATCGAT 1 cut(s) 880
BtgZI GCGATG 1 cut(s) 737
BtsCI GGATG 5 cut(s) 18, 216, 673, 751, 823
BtsIMutI CAGTG 1 cut(s) 786
BveI ACCTGC 1 cut(s) 576
Cac8I GCNNGC 4 cut(s) 328, 332, 376, 1176
CciI TCATGA 1 cut(s) 537
CfoI GCGC 4 cut(s) 15, 246, 390, 722
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 3 cut(s) 186, 508, 892
ClaI ATCGAT 1 cut(s) 880
CseI GACGC 3 cut(s) 257, 295, 332
Csp6I GTAC 1 cut(s) 919
CviAII CATG 8 cut(s) 457, 538, 739, 788, 835, 938, 983, 1175
CviQI GTAC 1 cut(s) 919
DdeI CTNAG 2 cut(s) 566, 1017
DinI GGCGCC 1 cut(s) 14
DpnI GATC 6 cut(s) 440, 702, 879, 883, 1140, 1212
DpnII GATC 6 cut(s) 438, 700, 877, 881, 1138, 1210
EaeI YGGCCR 1 cut(s) 361
EagI CGGCCG 1 cut(s) 361
Eam1104I CTCTTC 4 cut(s) 468, 668, 755, 959
EarI CTCTTC 4 cut(s) 468, 668, 755, 959
EciI GGCGGA 2 cut(s) 302, 535
EclXI CGGCCG 1 cut(s) 361
Eco47I GGWCC 3 cut(s) 186, 508, 892
Eco52I CGGCCG 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 627
EcoRI GAATTC 1 cut(s) 600
EcoRII CCWGG 1 cut(s) 1143
EgeI GGCGCC 1 cut(s) 14
EheI GGCGCC 1 cut(s) 14
FaeI CATG 8 cut(s) 460, 541, 742, 791, 838, 941, 986, 1178
FaqI GGGAC 3 cut(s) 632, 1084, 1209
FatI CATG 8 cut(s) 456, 537, 738, 787, 834, 937, 982, 1174
FauI CCCGC 1 cut(s) 383
FbaI TGATCA 1 cut(s) 1210
Fnu4HI GCNGC 4 cut(s) 80, 296, 361, 1046
FokI GGATG 5 cut(s) 5, 203, 680, 758, 830
Fsp4HI GCNGC 4 cut(s) 80, 296, 361, 1046
FspBI CTAG 2 cut(s) 875, 1115
GlaI GCGC 4 cut(s) 14, 245, 389, 721
GluI GCNGC 4 cut(s) 80, 296, 361, 1046
GsaI CCCAGC 1 cut(s) 1071
GsuI CTGGAG 2 cut(s) 368, 827
HaeII RGCGCY 2 cut(s) 16, 391
HaeIII GGCC 1 cut(s) 363
HapII CCGG 1 cut(s) 327
HgaI GACGC 3 cut(s) 257, 295, 332
HhaI GCGC 4 cut(s) 15, 246, 390, 722
Hin1I GRCGYC 3 cut(s) 13, 249, 324
Hin1II CATG 8 cut(s) 460, 541, 742, 791, 838, 941, 986, 1178
Hin6I GCGC 4 cut(s) 13, 244, 388, 720
HinP1I GCGC 4 cut(s) 13, 244, 388, 720
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HinfI GANTC 6 cut(s) 261, 483, 494, 513, 802, 901
HpaII CCGG 1 cut(s) 327
HphI GGTGA 7 cut(s) 42, 47, 124, 259, 439, 703, 727
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 5 cut(s) 155, 186, 567, 1032, 1098
Hpy188III TCNNGA 9 cut(s) 199, 406, 538, 605, 649, 806, 875, 885, 905
Hpy8I GTNNAC 1 cut(s) 517
Hpy99I CGWCG 4 cut(s) 242, 251, 323, 326
HpyAV CCTTC 5 cut(s) 133, 163, 787, 1129, 1180
HpyCH4IV ACGT 1 cut(s) 1055
HpyCH4V TGCA 2 cut(s) 585, 937
HpyF10VI GCNNNNNNNGC 6 cut(s) 312, 348, 357, 719, 859, 1042
HpyF3I CTNAG 2 cut(s) 566, 1017
HpySE526I ACGT 1 cut(s) 1055
Hsp92I GRCGYC 3 cut(s) 13, 249, 324
Hsp92II CATG 8 cut(s) 460, 541, 742, 791, 838, 941, 986, 1178
HspAI GCGC 4 cut(s) 13, 244, 388, 720
KasI GGCGCC 1 cut(s) 12
KroI GCCGGC 1 cut(s) 326
KroNI GCCGGC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 1210
Kzo9I GATC 6 cut(s) 438, 700, 877, 881, 1138, 1210
LmnI GCTCC 2 cut(s) 383, 478
Lsp1109I GCAGC 3 cut(s) 91, 307, 1057
LweI GCATC 1 cut(s) 1023
MaeI CTAG 2 cut(s) 875, 1115
MaeII ACGT 1 cut(s) 1055
MalI GATC 6 cut(s) 440, 702, 879, 883, 1140, 1212
MbiI CCGCTC 1 cut(s) 378
MboI GATC 6 cut(s) 438, 700, 877, 881, 1138, 1210
MboII GAAGA 6 cut(s) 394, 485, 620, 685, 772, 976
MflI RGATCY 2 cut(s) 700, 1138
MluCI AATT 7 cut(s) 149, 530, 600, 640, 694, 827, 1081
Mly113I GGCGCC 1 cut(s) 13
MlyI GAGTC 5 cut(s) 488, 492, 522, 811, 895
MmeI TCCRAC 1 cut(s) 486
MroNI GCCGGC 1 cut(s) 326
MseI TTAA 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 853
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 1145
MvaI CCWGG 1 cut(s) 1145
MvnI CGCG 5 cut(s) 246, 306, 342, 360, 722
MwoI GCNNNNNNNGC 6 cut(s) 312, 348, 357, 719, 859, 1042
NaeI GCCGGC 1 cut(s) 328
NarI GGCGCC 1 cut(s) 13
NdeII GATC 6 cut(s) 438, 700, 877, 881, 1138, 1210
NgoMIV GCCGGC 1 cut(s) 326
NlaIII CATG 8 cut(s) 460, 541, 742, 791, 838, 941, 986, 1178
NlaIV GGNNCC 3 cut(s) 14, 702, 1140
NmeAIII GCCGAG 2 cut(s) 327, 937
NspI RCATGY 2 cut(s) 838, 1178
NspV TTCGAA 2 cut(s) 228, 629
PaeI GCATGC 1 cut(s) 1178
PagI TCATGA 1 cut(s) 537
PciI ACATGT 1 cut(s) 834
PcsI WCGNNNNNNNCGW 3 cut(s) 234, 243, 447
PdiI GCCGGC 1 cut(s) 328
PfeI GAWTC 1 cut(s) 261
PflFI GACNNNGTC 1 cut(s) 512
PflMI CCANNNNNTGG 1 cut(s) 988
PkrI GCNGC 4 cut(s) 81, 297, 362, 1047
PleI GAGTC 5 cut(s) 488, 491, 521, 810, 895
PluTI GGCGCC 1 cut(s) 16
PpsI GAGTC 5 cut(s) 488, 491, 521, 810, 895
PscI ACATGT 1 cut(s) 834
Psp1406I AACGTT 1 cut(s) 1055
Psp6I CCWGG 1 cut(s) 1143
PspFI CCCAGC 1 cut(s) 1067
PspGI CCWGG 1 cut(s) 1143
PspN4I GGNNCC 3 cut(s) 14, 702, 1140
PspPI GGNCC 3 cut(s) 186, 508, 892
PsuI RGATCY 2 cut(s) 700, 1138
PsyI GACNNNGTC 1 cut(s) 512
RsaI GTAC 1 cut(s) 920
RsaNI GTAC 1 cut(s) 919
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 4 cut(s) 80, 296, 361, 1046
Sau3AI GATC 6 cut(s) 438, 700, 877, 881, 1138, 1210
Sau96I GGNCC 3 cut(s) 186, 508, 892
SchI GAGTC 5 cut(s) 488, 492, 522, 811, 895
ScrFI CCNGG 1 cut(s) 1145
SfaNI GCATC 1 cut(s) 1023
SfoI GGCGCC 1 cut(s) 14
SfuI TTCGAA 2 cut(s) 228, 629
SinI GGWCC 3 cut(s) 186, 508, 892
SphI GCATGC 1 cut(s) 1178
Sse9I AATT 7 cut(s) 149, 530, 600, 640, 694, 827, 1081
SsiI CCGC 9 cut(s) 304, 313, 340, 360, 376, 415, 546, 713, 851
SspDI GGCGCC 1 cut(s) 12
SspMI CTAG 2 cut(s) 875, 1115
StyD4I CCNGG 1 cut(s) 1143
TaiI ACGT 1 cut(s) 1058
TaqI TCGA 9 cut(s) 228, 240, 259, 318, 471, 492, 590, 629, 880
TaqII GACCGA 1 cut(s) 525
TasI AATT 7 cut(s) 149, 530, 600, 640, 694, 827, 1081
TatI WGTACW 1 cut(s) 918
TauI GCSGC 1 cut(s) 363
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 786
TseI GCWGC 3 cut(s) 79, 295, 1045
TspDTI ATGAA 4 cut(s) 445, 727, 1094, 1199
TspGWI ACGGA 2 cut(s) 693, 1088
TspRI CASTG 1 cut(s) 786
Tth111I GACNNNGTC 1 cut(s) 512
Van91I CCANNNNNTGG 1 cut(s) 988
VpaK11BI GGWCC 3 cut(s) 186, 508, 892
XapI RAATTY 2 cut(s) 149, 600
XbaI TCTAGA 1 cut(s) 874
XceI RCATGY 2 cut(s) 838, 1178
XspI CTAG 2 cut(s) 875, 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.