pycom08g20380

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0020388
Physical Location & Seq
Reverse (-)
20199422 .. 20200723
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1302 bp
ATGGGGGGTGATGATGGAGAATTGCCGATCTTCTCCTCCAGCAATGACGATATATTCGTCGAGATACTTTCATGGCTACCGGTCATATCTCTCCTCCGGTTCCGTTGTGTCTGCAAGTCATGGCGCGACTTAATCTCTACTTCCCAGTTCGTCGCCAAGCACCGAGCACATACCAACGACAACAACAAGAATGTCCTGATTCTCTTTAAACGCCCCTATCCACTTCCCCATGACTGGCCACCAATCCCCTTTCGATTTCCCCGACTCACTGACTACCAATCACTGAAGAAGAATGTTGGCGCCACTTCTGCAGCCGCATCATCAGGAATACGAAGCAGCCGTAGAGACCTTGAAGATGAATTTGCATTTGATACTAGTCTCTACAGTTCATCCCTTGTGGGTTCTTCCAATGGCCTCATCTGCCTACTACTTGACTGGCCTCGGCCACCTCGTGACTACTATAGGTGCTACGTAAACACAAGACTTCTGTTGTGGAACCCTAGTACCAGAACAGCCCGTAAGTTACCAGACATTGATTGGTTTCGTTACAACCCGTTTTATTATGGATTCGGCTATGATTCCATCACTCAAGACTACAAGGTCCTTTTGGGCGGTCAAGATCCTAACGGTTGTGAATTGGTTGCAATCTTCGCACTCAAAACGGGTTCATGGAAGAGTGTTGAAGATCCCCTCGGCCTCGAGGCGGCCTCGTTAGATATTCATGATCGAGGGTGCTTCTTAAATGGAGCTCTACATTGGCATGAGTATTATTGTTATGAAGATGAGGATTGGAATGAAGAGGAAACAGGTTATATGAGAACAAGAATCGTATCCTTTGATTTCGCGCAGGAGAAATTTCATTCGTTTTCCTTTATTAAGCATAAAGGACATATGGGGATGGCGGGGGGAGTGGGGACTATTGGAAATCGTCTCTTCTTTTACACTTCCTGCTATACTTCCACAAATCGTCCAGACTTGGAAGAGGCTGGCCTTACCATATGGGTGATGAGGGAATATGGGGTCGATCAATCTTGGACTATTTTTGCGAAAATCCGACCGGAGATTTTCCCTCCAAACGTGAAGTGGTTGAATCCTATATTTCATATGGAAGACGATGGTGCAGTTTTGATGCAATCGAGTCAAGGTGACTTGGTATTATATACGCGTAAGGAAGAGAGAGAGGAAGAAGACACATGCAGGATTGTGCTCAAGATTTCTGATGTTGGAGAATTGCCGGCTAGGTACGAAGAGACGGTTATGTACGCGGACACCTTGGTTTCACCGGTGATTGGCGGCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

434

Amino Acids

49.67

Weight (kDa)

5.25

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 16 - 50 8.9e-10 F-box domain
F-box-like PF12937 16 - 48 3.8e-07 F-box-like
FBA_3 PF08268 131 - 345 3.3e-15 F-box associated beta propeller domain
FBA_1 PF07734 158 - 426 2.7e-19 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020388)

Species Orthologous Gene IDs
malus_domestica MD08G1201200.v1.1
pyrus_communis pycom01g23620 pycom08g17460 pycom08g17480 pycom08g20380 pycom08g20390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 599
AbsI CCTCGAGG 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 299
AccII CGCG 4 cut(s) 126, 845, 1165, 1265
AciI CCGC 7 cut(s) 315, 612, 704, 902, 1265, 1293, 1296
AclWI GGATC 2 cut(s) 614, 680
AcoI YGGCCR 2 cut(s) 236, 443
AcsI RAATTY 2 cut(s) 359, 854
AcuI CTGAAG 1 cut(s) 305
AcyI GRCGYC 1 cut(s) 300
AdeI CACNNNGTG 1 cut(s) 452
AfaI GTAC 3 cut(s) 505, 1244, 1262
AfiI CCNNNNNNNGG 3 cut(s) 234, 703, 1289
AflIII ACRYGT 1 cut(s) 1163
AgeI ACCGGT 2 cut(s) 79, 1282
AgsI TTSAA 3 cut(s) 353, 683, 1090
AhlI ACTAGT 1 cut(s) 374
AluBI AGCT 1 cut(s) 749
AluI AGCT 1 cut(s) 749
Alw21I GWGCWC 3 cut(s) 169, 751, 1209
Alw26I GTCTC 4 cut(s) 339, 383, 935, 1244
AlwI GGATC 2 cut(s) 614, 680
Ama87I CYCGRG 1 cut(s) 698
AoxI GGCC 7 cut(s) 236, 412, 437, 443, 694, 705, 988
ApeKI GCWGC 2 cut(s) 311, 336
ApoI RAATTY 2 cut(s) 359, 854
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AsiGI ACCGGT 2 cut(s) 79, 1282
AspLEI GCGC 3 cut(s) 126, 302, 847
AspS9I GGNCC 1 cut(s) 601
AsuHPI GGTGA 5 cut(s) 20, 1015, 1157, 1272, 1297
AvaI CYCGRG 1 cut(s) 698
AvaII GGWCC 1 cut(s) 601
BaeI ACNNNNGTAYC 2 cut(s) 487, 520
BalI TGGCCA 1 cut(s) 238
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 751
BauI CACGAG 1 cut(s) 450
BbsI GAAGAC 2 cut(s) 1116, 1194
Bbv12I GWGCWC 3 cut(s) 169, 751, 1209
BbvI GCAGC 2 cut(s) 323, 348
BccI CCATC 4 cut(s) 8, 590, 892, 1109
BceAI ACGGC 1 cut(s) 324
BciVI GTATCC 1 cut(s) 841
BcoDI GTCTC 4 cut(s) 339, 383, 935, 1244
BcuI ACTAGT 1 cut(s) 374
BfaI CTAG 4 cut(s) 375, 501, 1239, 1300
BfmI CTRYAG 3 cut(s) 309, 382, 460
BfoI RGCGCY 1 cut(s) 303
BfuI GTATCC 1 cut(s) 841
BisI GCNGC 6 cut(s) 312, 315, 337, 705, 1294, 1297
BlsI GCNGC 6 cut(s) 313, 316, 338, 706, 1295, 1298
Bme18I GGWCC 1 cut(s) 601
BmeT110I CYCGRG 1 cut(s) 698
BmgT120I GGNCC 1 cut(s) 601
BmiI GGNNCC 3 cut(s) 101, 301, 497
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 2 cut(s) 326, 1119
BmuI ACTGGG 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 1116, 1194
BplI GAGNNNNNCTC 2 cut(s) 692, 724
BpmI CTGGAG 1 cut(s) 22
BpuEI CTTGAG 2 cut(s) 573, 1193
BsaAI YACGTR 1 cut(s) 472
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 440, 691, 1272
BsaWI WCCGGW 4 cut(s) 79, 96, 1057, 1282
Bsc4I CCNNNNNNNGG 3 cut(s) 234, 703, 1289
Bse118I RCCGGY 3 cut(s) 79, 1234, 1282
Bse1I ACTGG 3 cut(s) 145, 239, 440
Bse3DI GCAATG 1 cut(s) 49
BseDI CCNNGG 3 cut(s) 440, 691, 1272
BseGI GGATG 2 cut(s) 389, 903
BseLI CCNNNNNNNGG 3 cut(s) 234, 703, 1289
BseMI GCAATG 1 cut(s) 49
BseNI ACTGG 3 cut(s) 145, 239, 440
BseRI GAGGAG 2 cut(s) 25, 83
BseXI GCAGC 2 cut(s) 323, 348
BsgI GTGCAG 1 cut(s) 1140
Bsh1236I CGCG 4 cut(s) 126, 845, 1165, 1265
Bsh1285I CGRYCG 1 cut(s) 1058
BshFI GGCC 7 cut(s) 238, 414, 439, 445, 696, 707, 990
BshNI GGYRCC 1 cut(s) 299
BshTI ACCGGT 2 cut(s) 79, 1282
BsiEI CGRYCG 1 cut(s) 1058
BsiHKAI GWGCWC 3 cut(s) 169, 751, 1209
BsiHKCI CYCGRG 1 cut(s) 698
BsiSI CCGG 5 cut(s) 80, 97, 1058, 1235, 1283
BslFI GGGAC 1 cut(s) 928
BslI CCNNNNNNNGG 3 cut(s) 234, 703, 1289
BsmAI GTCTC 4 cut(s) 339, 383, 935, 1244
BsmBI CGTCTC 2 cut(s) 935, 1244
BsmFI GGGAC 1 cut(s) 928
BsnI GGCC 7 cut(s) 238, 414, 439, 445, 696, 707, 990
Bso31I GGTCTC 1 cut(s) 339
BsoBI CYCGRG 1 cut(s) 698
Bsp1286I GDGCHC 3 cut(s) 169, 751, 1209
Bsp143I GATC 5 cut(s) 27, 619, 685, 724, 1024
BspACI CCGC 7 cut(s) 315, 612, 704, 902, 1265, 1293, 1296
BspANI GGCC 7 cut(s) 238, 414, 439, 445, 696, 707, 990
BspFNI CGCG 4 cut(s) 126, 845, 1165, 1265
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 3 cut(s) 101, 301, 497
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 2 cut(s) 614, 680
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 49
BsrFI RCCGGY 3 cut(s) 79, 1234, 1282
BsrI ACTGG 3 cut(s) 145, 239, 440
BssAI RCCGGY 3 cut(s) 79, 1234, 1282
BssECI CCNNGG 3 cut(s) 440, 691, 1272
BssMI GATC 5 cut(s) 27, 619, 685, 724, 1024
BssNI GRCGYC 1 cut(s) 300
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 1272
Bst2BI CACGAG 1 cut(s) 450
Bst4CI ACNGT 3 cut(s) 386, 629, 1255
Bst6I CTCTTC 6 cut(s) 668, 792, 938, 975, 1167, 1242
BstACI GRCGYC 1 cut(s) 300
BstBAI YACGTR 1 cut(s) 472
BstC8I GCNNGC 2 cut(s) 988, 1236
BstF5I GGATG 2 cut(s) 389, 903
BstFNI CGCG 4 cut(s) 126, 845, 1165, 1265
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 3 cut(s) 126, 302, 847
BstKTI GATC 5 cut(s) 30, 622, 688, 727, 1027
BstMAI GTCTC 4 cut(s) 339, 383, 935, 1244
BstMBI GATC 5 cut(s) 27, 619, 685, 724, 1024
BstMCI CGRYCG 1 cut(s) 1058
BstMWI GCNNNNNNNGC 3 cut(s) 308, 420, 650
BstNSI RCATGY 1 cut(s) 1197
BstSFI CTRYAG 3 cut(s) 309, 382, 460
BstSNI TACGTA 1 cut(s) 472
BstUI CGCG 4 cut(s) 126, 845, 1165, 1265
BstV1I GCAGC 2 cut(s) 323, 348
BstV2I GAAGAC 2 cut(s) 1116, 1194
BstX2I RGATCY 2 cut(s) 619, 685
BstYI RGATCY 2 cut(s) 619, 685
BsuI GTATCC 1 cut(s) 841
BsuRI GGCC 7 cut(s) 238, 414, 439, 445, 696, 707, 990
BtsCI GGATG 2 cut(s) 389, 903
BtsIMutI CAGTG 2 cut(s) 267, 281
Cac8I GCNNGC 2 cut(s) 988, 1236
CciI TCATGA 1 cut(s) 721
CfoI GCGC 3 cut(s) 126, 302, 847
Cfr10I RCCGGY 3 cut(s) 79, 1234, 1282
Cfr13I GGNCC 1 cut(s) 601
Csp6I GTAC 3 cut(s) 504, 1243, 1261
CspAI ACCGGT 2 cut(s) 79, 1282
CviAII CATG 7 cut(s) 72, 120, 230, 669, 722, 761, 1194
CviQI GTAC 3 cut(s) 504, 1243, 1261
DinI GGCGCC 1 cut(s) 301
DpnI GATC 5 cut(s) 29, 621, 687, 726, 1026
DpnII GATC 5 cut(s) 27, 619, 685, 724, 1024
DraI TTTAAA 1 cut(s) 208
DraIII CACNNNGTG 1 cut(s) 452
DrdI GACNNNNNNGTC 1 cut(s) 599
DseDI GACNNNNNNGTC 1 cut(s) 599
EaeI YGGCCR 2 cut(s) 236, 443
Eam1104I CTCTTC 6 cut(s) 668, 792, 938, 975, 1167, 1242
EarI CTCTTC 6 cut(s) 668, 792, 938, 975, 1167, 1242
Ecl136II GAGCTC 1 cut(s) 749
Eco105I TACGTA 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 1272
Eco24I GRGCYC 1 cut(s) 751
Eco31I GGTCTC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 601
Eco53kI GAGCTC 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 698
EcoICRI GAGCTC 1 cut(s) 749
EcoO109I RGGNCCY 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 1272
EcoT38I GRGCYC 1 cut(s) 751
EgeI GGCGCC 1 cut(s) 301
EheI GGCGCC 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 1272
Esp3I CGTCTC 2 cut(s) 935, 1244
FaeI CATG 7 cut(s) 75, 123, 233, 672, 725, 764, 1197
FaqI GGGAC 1 cut(s) 928
FatI CATG 7 cut(s) 71, 119, 229, 668, 721, 760, 1193
FauI CCCGC 1 cut(s) 895
FauNDI CATATG 3 cut(s) 891, 998, 1104
Fnu4HI GCNGC 6 cut(s) 312, 315, 337, 705, 1294, 1297
FokI GGATG 2 cut(s) 376, 910
FriOI GRGCYC 1 cut(s) 751
Fsp4HI GCNGC 6 cut(s) 312, 315, 337, 705, 1294, 1297
FspBI CTAG 4 cut(s) 375, 501, 1239, 1300
GlaI GCGC 3 cut(s) 125, 301, 846
GluI GCNGC 6 cut(s) 312, 315, 337, 705, 1294, 1297
GsuI CTGGAG 1 cut(s) 22
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 7 cut(s) 238, 414, 439, 445, 696, 707, 990
HapII CCGG 5 cut(s) 80, 97, 1058, 1235, 1283
HhaI GCGC 3 cut(s) 126, 302, 847
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 7 cut(s) 75, 123, 233, 672, 725, 764, 1197
Hin6I GCGC 3 cut(s) 124, 300, 845
HinP1I GCGC 3 cut(s) 124, 300, 845
HinfI GANTC 7 cut(s) 199, 264, 567, 578, 825, 1090, 1138
HpaII CCGG 5 cut(s) 80, 97, 1058, 1235, 1283
HphI GGTGA 5 cut(s) 20, 1015, 1157, 1272, 1297
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 2 cut(s) 1055, 1219
Hpy188III TCNNGA 9 cut(s) 61, 196, 324, 452, 590, 617, 722, 971, 1210
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 2 cut(s) 62, 155
HpyCH4III ACNGT 3 cut(s) 386, 629, 1255
HpyCH4IV ACGT 2 cut(s) 471, 1077
HpyCH4V TGCA 7 cut(s) 114, 311, 365, 644, 1121, 1132, 1197
HpyF10VI GCNNNNNNNGC 3 cut(s) 308, 420, 650
HpySE526I ACGT 2 cut(s) 471, 1077
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 7 cut(s) 75, 123, 233, 672, 725, 764, 1197
HspAI GCGC 3 cut(s) 124, 300, 845
KasI GGCGCC 1 cut(s) 299
KroI GCCGGC 1 cut(s) 1234
KroNI GCCGGC 1 cut(s) 1236
Kzo9I GATC 5 cut(s) 27, 619, 685, 724, 1024
LmnI GCTCC 1 cut(s) 746
Lsp1109I GCAGC 2 cut(s) 323, 348
LweI GCATC 2 cut(s) 326, 1119
MaeI CTAG 4 cut(s) 375, 501, 1239, 1300
MaeII ACGT 2 cut(s) 471, 1077
MaeIII GTNAC 4 cut(s) 452, 522, 545, 1145
MalI GATC 5 cut(s) 29, 621, 687, 726, 1026
MboI GATC 5 cut(s) 27, 619, 685, 724, 1024
MflI RGATCY 2 cut(s) 619, 685
MhlI GDGCHC 3 cut(s) 169, 751, 1209
MlsI TGGCCA 1 cut(s) 238
MluCI AATT 5 cut(s) 20, 359, 635, 854, 1229
MluI ACGCGT 1 cut(s) 1163
MluNI TGGCCA 1 cut(s) 238
Mly113I GGCGCC 1 cut(s) 300
MlyI GAGTC 2 cut(s) 258, 1147
MmeI TCCRAC 2 cut(s) 1078, 1204
Mox20I TGGCCA 1 cut(s) 238
MroNI GCCGGC 1 cut(s) 1234
MscI TGGCCA 1 cut(s) 238
MseI TTAA 4 cut(s) 131, 207, 740, 876
MslI CAYNNNNRTG 2 cut(s) 759, 1001
Msp20I TGGCCA 1 cut(s) 238
MspI CCGG 5 cut(s) 80, 97, 1058, 1235, 1283
MvnI CGCG 4 cut(s) 126, 845, 1165, 1265
MwoI GCNNNNNNNGC 3 cut(s) 308, 420, 650
NaeI GCCGGC 1 cut(s) 1236
NarI GGCGCC 1 cut(s) 300
NdeI CATATG 3 cut(s) 891, 998, 1104
NdeII GATC 5 cut(s) 27, 619, 685, 724, 1024
NgoMIV GCCGGC 1 cut(s) 1234
NlaIII CATG 7 cut(s) 75, 123, 233, 672, 725, 764, 1197
NlaIV GGNNCC 3 cut(s) 101, 301, 497
NmeAIII GCCGAG 2 cut(s) 421, 672
NmuCI GTSAC 2 cut(s) 452, 1145
NspI RCATGY 1 cut(s) 1197
PaeR7I CTCGAG 1 cut(s) 698
PagI TCATGA 1 cut(s) 721
PcsI WCGNNNNNNNCGW 4 cut(s) 54, 259, 337, 448
PdiI GCCGGC 1 cut(s) 1236
PfeI GAWTC 5 cut(s) 199, 567, 578, 825, 1090
PinAI ACCGGT 2 cut(s) 79, 1282
PkrI GCNGC 6 cut(s) 313, 316, 338, 706, 1295, 1298
PleI GAGTC 2 cut(s) 258, 1146
PluTI GGCGCC 1 cut(s) 303
PpsI GAGTC 2 cut(s) 258, 1146
Ppu21I YACGTR 1 cut(s) 472
PpuMI RGGWCCY 1 cut(s) 601
Psp124BI GAGCTC 1 cut(s) 751
Psp5II RGGWCCY 1 cut(s) 601
PspN4I GGNNCC 3 cut(s) 101, 301, 497
PspPI GGNCC 1 cut(s) 601
PspPPI RGGWCCY 1 cut(s) 601
PspXI VCTCGAGB 1 cut(s) 698
PstI CTGCAG 1 cut(s) 313
PsuI RGATCY 2 cut(s) 619, 685
RsaI GTAC 3 cut(s) 505, 1244, 1262
RsaNI GTAC 3 cut(s) 504, 1243, 1261
RseI CAYNNNNRTG 2 cut(s) 759, 1001
SacI GAGCTC 1 cut(s) 751
SaqAI TTAA 4 cut(s) 131, 207, 740, 876
SatI GCNGC 6 cut(s) 312, 315, 337, 705, 1294, 1297
Sau3AI GATC 5 cut(s) 27, 619, 685, 724, 1024
Sau96I GGNCC 1 cut(s) 601
SchI GAGTC 2 cut(s) 258, 1147
SduI GDGCHC 3 cut(s) 169, 751, 1209
SfaNI GCATC 2 cut(s) 326, 1119
SfcI CTRYAG 3 cut(s) 309, 382, 460
SfoI GGCGCC 1 cut(s) 301
Sfr274I CTCGAG 1 cut(s) 698
SgrAI CRCCGGYG 1 cut(s) 1282
SinI GGWCC 1 cut(s) 601
SlaI CTCGAG 1 cut(s) 698
SmiMI CAYNNNNRTG 2 cut(s) 759, 1001
SmlI CTYRAG 3 cut(s) 588, 698, 1208
SmoI CTYRAG 3 cut(s) 588, 698, 1208
SnaBI TACGTA 1 cut(s) 472
SpeI ACTAGT 1 cut(s) 374
Sse9I AATT 5 cut(s) 20, 359, 635, 854, 1229
SsiI CCGC 7 cut(s) 315, 612, 704, 902, 1265, 1293, 1296
SspDI GGCGCC 1 cut(s) 299
SspMI CTAG 4 cut(s) 375, 501, 1239, 1300
SstI GAGCTC 1 cut(s) 751
StyI CCWWGG 1 cut(s) 1272
TaaI ACNGT 3 cut(s) 386, 629, 1255
TaiI ACGT 2 cut(s) 474, 1080
TaqI TCGA 6 cut(s) 60, 253, 699, 727, 1023, 1136
TasI AATT 5 cut(s) 20, 359, 635, 854, 1229
TauI GCSGC 4 cut(s) 317, 707, 1296, 1299
TfiI GAWTC 5 cut(s) 199, 567, 578, 825, 1090
Tru1I TTAA 4 cut(s) 131, 207, 740, 876
Tru9I TTAA 4 cut(s) 131, 207, 740, 876
TscAI CASTG 2 cut(s) 274, 288
TseFI GTSAC 2 cut(s) 452, 1145
TseI GCWGC 2 cut(s) 311, 336
Tsp45I GTSAC 2 cut(s) 452, 1145
TspDTI ATGAA 9 cut(s) 60, 372, 378, 657, 710, 792, 810, 848, 1091
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 2 cut(s) 274, 288
VpaK11BI GGWCC 1 cut(s) 601
XapI RAATTY 2 cut(s) 359, 854
XceI RCATGY 1 cut(s) 1197
XcmI CCANNNNNNNNNTGG 2 cut(s) 534, 1080
XhoI CTCGAG 1 cut(s) 698
XspI CTAG 4 cut(s) 375, 501, 1239, 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.