pycom09g03730

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0009531
Physical Location & Seq
Reverse (-)
2749006 .. 2751499
2494 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1698 bp
ATGGCTTGCTGCAGGGCTTATTTTGGGACTGTTGCAGGAATTGAACTAGCTGAGAGGATATGTGTGATGGGAATTTCTATGAATTTGGTAACATACTTGGTTGGAAATTTGCATATATCTTCTGCAAAATCTGCAAACATAGTGACCAATTTCATGGGCACTTTGAACCTCCTTGGCCTCCTTGGTGGGTTTCTAGCAGATGCTAAGCTTGGAAGGTACCTCACAGTTGCACTTTCTGCAACCATAACGGCTGTGGGGATAATATTGTTGACATTGGCGACATCGATTCATAGCATGAGACCACCTCCATGTGATGACTACAGACAGCAACACCATCAATGCATTGAGGCCAACGGAACCCAATTAGCCCTACTCTATGCTGCTTTGTACACTACAGCACTGGGTGGTGGTGGCATAAAATCCAACGTCTCTGGTTTTGGGTCCGATCAATTCGACGTTGCGGACCCCAAGGAAGAGAAGGCCATGATCTTTTTCTTCAACAGGTTCTACTTCGGCATCAGCATTGGGTCTCTGTTTGCTGTCATTGTGCTGGTGTATGTGCAAGTCAATGTTAGCAGAGGGTTAGGTTATGGAATAGCGGCTGGGGCGATGATCATAGCGATCATCGTATTGCTCGCGGGAACGACGTTTTATCGGTTCAGCAAGCCTCGAGGCAGCCCTTTAACTGTCATATGGAGAGTGATATTTTTGGCTTGGAAGAAGAGAGCTTATCCTCTTCCTTCTCATCCAAGCCTTTTGAATGGATGCCAAGAAGCCAAGATCCCCCACACAGAGAGGCTCAAGTGTCTGGACAAAGCTGCAATCCTAGATGAATATGCTGCTCATGAAGGAAACAGAAGCAACTCATGGATAGTATCCACAGTGACCCAAGTCGAAGAGGTGAAACTGGTTTTCAAGCTCATGTCGATCTGGTCGACATGTATCCTCTTCTGGACGGTCTACTCTCAGATGACTACATTCACCATTGAACAAGCCACTTTCATGAACCGAAAAGTAGGCTCCTTTGAAATCCCAGCGGGTTCTTTCTCGGTGTTTCTATTCATCACCATCCTCATCTTTACTTCCTTAAACGAGAAACTATTTGTCCCCCTTGCTCGGAAACTCACCAACAATGCCCAAGGAATAACAAGCCTTCAGAGGATTGGAGTTGGACTCATTTTATCAATGGCGGCTATGGTGGCTTCCGCAATAGTAGAGAAAGAAAGAAGGAACATTGCAGTTCACCAAAATACCAAACTAAGAGCTTTCTGGCTTATCCCACAATATTTCCTCGTGGGTGCAGGGGAAGCTTTTGTCTACGTCGGACAACTTGAGTTTTTCATTAGGGAGGCTCCGGACAGGATGAAGTCCATGAGCACGGGGCTTTTCTTAAGCACCATCTCAATGGGATTCTACGTTAGCAGTCTTTTGGTGACGGTAGTGGACAAAGTGACTAGGAAGAGCTGGCTGAGGAGTAACTTGAACAAGGGAAGGGTAGATAACTTCTACTGGGTCCTCGCGGTGCTAGGAGCTACAAATTTTTTAGTTTTTCTTGCCTTTGCAATGAGACACCAATACAAAGGCCATCAACACAACAGCCCTGATGATAGTGGCGAAAAGGAGTTCAAAAGCTCGAGCATCAAAACAGTTGAAGAGATGGCAGAGATAATCAGAATTGAGGCAAAGGAAGGACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

566

Amino Acids

62.47

Weight (kDa)

9.31

Isoelectric Point (pI)

27.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 73 - 486 4.9e-104 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 669
Acc65I GGTACC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 216
AccI GTMKAC 3 cut(s) 935, 960, 1317
AccII CGCG 2 cut(s) 638, 1520
AccIII TCCGGA 1 cut(s) 1354
AciI CCGC 7 cut(s) 461, 599, 638, 1037, 1190, 1206, 1520
AclWI GGATC 1 cut(s) 775
AcsI RAATTY 4 cut(s) 72, 82, 106, 1537
AcuI CTGAAG 1 cut(s) 1139
AdeI CACNNNGTG 1 cut(s) 404
AfaI GTAC 2 cut(s) 218, 389
AfiI CCNNNNNNNGG 1 cut(s) 1116
AflII CTTAAG 1 cut(s) 1390
AflIII ACRYGT 1 cut(s) 938
Alw21I GWGCWC 1 cut(s) 1379
Alw26I GTCTC 4 cut(s) 292, 433, 534, 1561
AlwI GGATC 1 cut(s) 775
Ama87I CYCGRG 2 cut(s) 669, 1633
Aor13HI TCCGGA 1 cut(s) 1354
AoxI GGCC 4 cut(s) 175, 348, 480, 1582
ApeKI GCWGC 5 cut(s) 9, 380, 675, 818, 839
ApoI RAATTY 4 cut(s) 72, 82, 106, 1537
Asp718I GGTACC 1 cut(s) 216
AspS9I GGNCC 4 cut(s) 441, 463, 1513, 1691
AsuHPI GGTGA 6 cut(s) 913, 973, 1057, 1117, 1235, 1444
AvaI CYCGRG 2 cut(s) 669, 1633
AvaII GGWCC 4 cut(s) 441, 463, 1513, 1691
BaeGI GKGCMC 1 cut(s) 161
BanI GGYRCC 1 cut(s) 216
BauI CACGAG 1 cut(s) 1292
Bbv12I GWGCWC 1 cut(s) 1379
BbvCI CCTCAGC 1 cut(s) 1469
BbvI GCAGC 4 cut(s) 367, 687, 805, 826
BccI CCATC 6 cut(s) 61, 342, 1076, 1406, 1593, 1651
BceAI ACGGC 1 cut(s) 264
BciVI GTATCC 2 cut(s) 886, 953
BclI TGATCA 1 cut(s) 612
BcoDI GTCTC 4 cut(s) 292, 433, 534, 1561
BfaI CTAG 5 cut(s) 47, 194, 827, 1455, 1526
BfmI CTRYAG 3 cut(s) 10, 319, 393
BfrI CTTAAG 1 cut(s) 1390
BfuI GTATCC 2 cut(s) 886, 953
BisI GCNGC 7 cut(s) 10, 381, 600, 676, 819, 840, 1191
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 7 cut(s) 11, 382, 601, 677, 820, 841, 1192
Bme18I GGWCC 4 cut(s) 441, 463, 1513, 1691
BmeT110I CYCGRG 2 cut(s) 669, 1633
BmgT120I GGNCC 4 cut(s) 441, 463, 1513, 1691
BmiI GGNNCC 7 cut(s) 218, 358, 442, 465, 1021, 1353, 1514
BmrI ACTGGG 2 cut(s) 410, 1519
BmsI GCATC 4 cut(s) 190, 525, 755, 1647
BmuI ACTGGG 2 cut(s) 410, 1519
BoxI GACNNNNGTC 1 cut(s) 890
BplI GAGNNNNNCTC 4 cut(s) 289, 321, 1158, 1190
Bpu10I CCTNAGC 1 cut(s) 1469
Bpu1102I GCTNAGC 1 cut(s) 204
BpuEI CTTGAG 2 cut(s) 785, 1352
Bsa29I ATCGAT 1 cut(s) 284
BsaI GGTCTC 2 cut(s) 292, 534
BsaJI CCNNGG 4 cut(s) 172, 181, 468, 1138
BsaWI WCCGGW 1 cut(s) 1354
Bsc4I CCNNNNNNNGG 1 cut(s) 1116
Bse1I ACTGG 3 cut(s) 405, 912, 1514
Bse3DI GCAATG 2 cut(s) 1233, 1569
BseAI TCCGGA 1 cut(s) 1354
BseCI ATCGAT 1 cut(s) 284
BseDI CCNNGG 4 cut(s) 172, 181, 468, 1138
BseGI GGATG 4 cut(s) 745, 770, 1068, 1368
BseLI CCNNNNNNNGG 1 cut(s) 1116
BseMI GCAATG 2 cut(s) 1233, 1569
BseMII CTCAG 3 cut(s) 42, 980, 1460
BseNI ACTGG 3 cut(s) 405, 912, 1514
BseRI GAGGAG 1 cut(s) 1486
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 4 cut(s) 367, 687, 805, 826
BseYI CCCAGC 2 cut(s) 602, 1033
BsgI GTGCAG 1 cut(s) 1320
Bsh1236I CGCG 2 cut(s) 638, 1520
BshFI GGCC 4 cut(s) 177, 350, 482, 1584
BshNI GGYRCC 1 cut(s) 216
BshVI ATCGAT 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 1379
BsiHKCI CYCGRG 2 cut(s) 669, 1633
BsiSI CCGG 1 cut(s) 1355
BslFI GGGAC 2 cut(s) 40, 1091
BslI CCNNNNNNNGG 1 cut(s) 1116
BsmAI GTCTC 4 cut(s) 292, 433, 534, 1561
BsmBI CGTCTC 1 cut(s) 433
BsmFI GGGAC 2 cut(s) 40, 1091
BsnI GGCC 4 cut(s) 177, 350, 482, 1584
Bso31I GGTCTC 2 cut(s) 292, 534
BsoBI CYCGRG 2 cut(s) 669, 1633
Bsp1286I GDGCHC 2 cut(s) 161, 1379
Bsp13I TCCGGA 1 cut(s) 1354
Bsp1407I TGTACA 1 cut(s) 387
Bsp143I GATC 6 cut(s) 445, 486, 612, 621, 780, 927
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 7 cut(s) 461, 599, 638, 1037, 1190, 1206, 1520
BspANI GGCC 4 cut(s) 177, 350, 482, 1584
BspCNI CTCAG 3 cut(s) 43, 979, 1461
BspDI ATCGAT 1 cut(s) 284
BspEI TCCGGA 1 cut(s) 1354
BspFNI CGCG 2 cut(s) 638, 1520
BspHI TCATGA 2 cut(s) 844, 1002
BspLI GGNNCC 7 cut(s) 218, 358, 442, 465, 1021, 1353, 1514
BspMAI CTGCAG 1 cut(s) 14
BspPI GGATC 1 cut(s) 775
BspQI GCTCTTC 1 cut(s) 1454
BspT107I GGYRCC 1 cut(s) 216
BspTI CTTAAG 1 cut(s) 1390
BspTNI GGTCTC 2 cut(s) 292, 534
BsrDI GCAATG 2 cut(s) 1233, 1569
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 3 cut(s) 405, 912, 1514
BssECI CCNNGG 4 cut(s) 172, 181, 468, 1138
BssMI GATC 6 cut(s) 445, 486, 612, 621, 780, 927
BssSI CACGAG 1 cut(s) 1292
BssT1I CCWWGG 4 cut(s) 172, 181, 468, 1138
Bst2BI CACGAG 1 cut(s) 1292
Bst4CI ACNGT 7 cut(s) 31, 226, 688, 883, 958, 1438, 1648
Bst6I CTCTTC 7 cut(s) 468, 716, 741, 891, 953, 1454, 1647
BstAFI CTTAAG 1 cut(s) 1390
BstAPI GCANNNNNTGC 2 cut(s) 131, 236
BstAUI TGTACA 1 cut(s) 387
BstC8I GCNNGC 4 cut(s) 7, 636, 665, 1466
BstDEI CTNAG 6 cut(s) 51, 204, 966, 1259, 1469, 1695
BstF5I GGATG 4 cut(s) 745, 770, 1068, 1368
BstFNI CGCG 2 cut(s) 638, 1520
BstKTI GATC 6 cut(s) 448, 489, 615, 624, 783, 930
BstMAI GTCTC 4 cut(s) 292, 433, 534, 1561
BstMBI GATC 6 cut(s) 445, 486, 612, 621, 780, 927
BstMWI GCNNNNNNNGC 5 cut(s) 131, 236, 605, 1199, 1307
BstNSI RCATGY 1 cut(s) 942
BstPAI GACNNNNGTC 1 cut(s) 890
BstSFI CTRYAG 3 cut(s) 10, 319, 393
BstSLI GKGCMC 1 cut(s) 161
BstUI CGCG 2 cut(s) 638, 1520
BstV1I GCAGC 4 cut(s) 367, 687, 805, 826
BstX2I RGATCY 1 cut(s) 780
BstXI CCANNNNNNTGG 2 cut(s) 154, 1405
BstYI RGATCY 1 cut(s) 780
Bsu15I ATCGAT 1 cut(s) 284
BsuI GTATCC 2 cut(s) 886, 953
BsuRI GGCC 4 cut(s) 177, 350, 482, 1584
BsuTUI ATCGAT 1 cut(s) 284
BtgZI GCGATG 1 cut(s) 623
BtsCI GGATG 4 cut(s) 745, 770, 1068, 1368
BtsIMutI CAGTG 2 cut(s) 398, 888
Cac8I GCNNGC 4 cut(s) 7, 636, 665, 1466
CciI TCATGA 2 cut(s) 844, 1002
Cfr13I GGNCC 4 cut(s) 441, 463, 1513, 1691
ClaI ATCGAT 1 cut(s) 284
Csp6I GTAC 2 cut(s) 217, 388
CviQI GTAC 2 cut(s) 217, 388
DdeI CTNAG 6 cut(s) 51, 204, 966, 1259, 1469, 1695
DpnI GATC 6 cut(s) 447, 488, 614, 623, 782, 929
DpnII GATC 6 cut(s) 445, 486, 612, 621, 780, 927
DraIII CACNNNGTG 1 cut(s) 404
Eam1104I CTCTTC 7 cut(s) 468, 716, 741, 891, 953, 1454, 1647
EarI CTCTTC 7 cut(s) 468, 716, 741, 891, 953, 1454, 1647
Eco130I CCWWGG 4 cut(s) 172, 181, 468, 1138
Eco31I GGTCTC 2 cut(s) 292, 534
Eco47I GGWCC 4 cut(s) 441, 463, 1513, 1691
Eco57I CTGAAG 1 cut(s) 1139
Eco88I CYCGRG 2 cut(s) 669, 1633
EcoO109I RGGNCCY 2 cut(s) 1513, 1691
EcoT14I CCWWGG 4 cut(s) 172, 181, 468, 1138
EcoT22I ATGCAT 1 cut(s) 344
ErhI CCWWGG 4 cut(s) 172, 181, 468, 1138
Esp3I CGTCTC 1 cut(s) 433
FaqI GGGAC 2 cut(s) 40, 1091
FauI CCCGC 2 cut(s) 631, 1030
FauNDI CATATG 1 cut(s) 692
FbaI TGATCA 1 cut(s) 612
FblI GTMKAC 3 cut(s) 935, 960, 1317
Fnu4HI GCNGC 7 cut(s) 10, 381, 600, 676, 819, 840, 1191
FokI GGATG 4 cut(s) 732, 777, 1055, 1375
Fsp4HI GCNGC 7 cut(s) 10, 381, 600, 676, 819, 840, 1191
FspBI CTAG 5 cut(s) 47, 194, 827, 1455, 1526
GluI GCNGC 7 cut(s) 10, 381, 600, 676, 819, 840, 1191
GsaI CCCAGC 2 cut(s) 606, 1037
HaeIII GGCC 4 cut(s) 177, 350, 482, 1584
HapII CCGG 1 cut(s) 1355
HincII GTYRAC 2 cut(s) 270, 936
HindII GTYRAC 2 cut(s) 270, 936
HindIII AAGCTT 2 cut(s) 206, 1308
HinfI GANTC 3 cut(s) 286, 1173, 1410
HpaII CCGG 1 cut(s) 1355
HphI GGTGA 6 cut(s) 913, 973, 1057, 1117, 1235, 1444
Hpy166II GTNNAC 7 cut(s) 270, 390, 936, 961, 1243, 1318, 1444
Hpy188I TCNGA 6 cut(s) 445, 969, 1119, 1158, 1325, 1673
Hpy188III TCNNGA 5 cut(s) 809, 845, 952, 1003, 1355
Hpy8I GTNNAC 7 cut(s) 270, 390, 936, 961, 1243, 1318, 1444
Hpy99I CGWCG 3 cut(s) 458, 649, 1325
HpyAV CCTTC 8 cut(s) 207, 472, 750, 842, 1163, 1221, 1485, 1682
HpyCH4III ACNGT 7 cut(s) 31, 226, 688, 883, 958, 1438, 1648
HpyCH4IV ACGT 5 cut(s) 426, 456, 647, 1320, 1416
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 236, 605, 1199, 1307
HpyF3I CTNAG 6 cut(s) 51, 204, 966, 1259, 1469, 1695
HpySE526I ACGT 5 cut(s) 426, 456, 647, 1320, 1416
Kpn2I TCCGGA 1 cut(s) 1354
KpnI GGTACC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 6 cut(s) 445, 486, 612, 621, 780, 927
LguI GCTCTTC 1 cut(s) 1454
LmnI GCTCC 3 cut(s) 1025, 1357, 1529
Lsp1109I GCAGC 4 cut(s) 367, 687, 805, 826
LweI GCATC 4 cut(s) 190, 525, 755, 1647
MaeI CTAG 5 cut(s) 47, 194, 827, 1455, 1526
MaeII ACGT 5 cut(s) 426, 456, 647, 1320, 1416
MaeIII GTNAC 6 cut(s) 88, 142, 883, 1432, 1450, 1475
MalI GATC 6 cut(s) 447, 488, 614, 623, 782, 929
MboI GATC 6 cut(s) 445, 486, 612, 621, 780, 927
MflI RGATCY 1 cut(s) 780
MhlI GDGCHC 2 cut(s) 161, 1379
MluCI AATT 9 cut(s) 39, 72, 82, 106, 148, 362, 449, 1537, 1674
MlyI GAGTC 1 cut(s) 1167
MmeI TCCRAC 4 cut(s) 82, 447, 1150, 1303
Mph1103I ATGCAT 1 cut(s) 344
MroI TCCGGA 1 cut(s) 1354
MseI TTAA 3 cut(s) 683, 1088, 1391
MslI CAYNNNNRTG 3 cut(s) 307, 1001, 1403
MspA1I CMGCKG 1 cut(s) 1037
MspCI CTTAAG 1 cut(s) 1390
MspI CCGG 1 cut(s) 1355
MvnI CGCG 2 cut(s) 638, 1520
MwoI GCNNNNNNNGC 5 cut(s) 131, 236, 605, 1199, 1307
NdeI CATATG 1 cut(s) 692
NdeII GATC 6 cut(s) 445, 486, 612, 621, 780, 927
NlaIV GGNNCC 7 cut(s) 218, 358, 442, 465, 1021, 1353, 1514
NmuCI GTSAC 4 cut(s) 142, 883, 1432, 1450
NsiI ATGCAT 1 cut(s) 344
NspI RCATGY 1 cut(s) 942
PaeR7I CTCGAG 2 cut(s) 669, 1633
PagI TCATGA 2 cut(s) 844, 1002
PciI ACATGT 1 cut(s) 938
PciSI GCTCTTC 1 cut(s) 1454
PcsI WCGNNNNNNNCGW 1 cut(s) 932
PfeI GAWTC 2 cut(s) 286, 1410
PkrI GCNGC 7 cut(s) 11, 382, 601, 677, 820, 841, 1192
PleI GAGTC 1 cut(s) 1167
PpsI GAGTC 1 cut(s) 1167
PpuMI RGGWCCY 2 cut(s) 1513, 1691
PscI ACATGT 1 cut(s) 938
PshAI GACNNNNGTC 1 cut(s) 890
Psp5II RGGWCCY 2 cut(s) 1513, 1691
PspFI CCCAGC 2 cut(s) 602, 1033
PspN4I GGNNCC 7 cut(s) 218, 358, 442, 465, 1021, 1353, 1514
PspPI GGNCC 4 cut(s) 441, 463, 1513, 1691
PspPPI RGGWCCY 2 cut(s) 1513, 1691
PspXI VCTCGAGB 2 cut(s) 669, 1633
PstI CTGCAG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 780
RsaI GTAC 2 cut(s) 218, 389
RsaNI GTAC 2 cut(s) 217, 388
RseI CAYNNNNRTG 3 cut(s) 307, 1001, 1403
SalI GTCGAC 1 cut(s) 934
SapI GCTCTTC 1 cut(s) 1454
SaqAI TTAA 3 cut(s) 683, 1088, 1391
SatI GCNGC 7 cut(s) 10, 381, 600, 676, 819, 840, 1191
Sau3AI GATC 6 cut(s) 445, 486, 612, 621, 780, 927
Sau96I GGNCC 4 cut(s) 441, 463, 1513, 1691
SchI GAGTC 1 cut(s) 1167
SduI GDGCHC 2 cut(s) 161, 1379
SfaNI GCATC 4 cut(s) 190, 525, 755, 1647
SfcI CTRYAG 3 cut(s) 10, 319, 393
Sfr274I CTCGAG 2 cut(s) 669, 1633
SinI GGWCC 4 cut(s) 441, 463, 1513, 1691
SlaI CTCGAG 2 cut(s) 669, 1633
SmiMI CAYNNNNRTG 3 cut(s) 307, 1001, 1403
SmlI CTYRAG 5 cut(s) 669, 800, 1331, 1390, 1633
SmoI CTYRAG 5 cut(s) 669, 800, 1331, 1390, 1633
Sse9I AATT 9 cut(s) 39, 72, 82, 106, 148, 362, 449, 1537, 1674
SsiI CCGC 7 cut(s) 461, 599, 638, 1037, 1190, 1206, 1520
SspI AATATT 2 cut(s) 264, 1286
SspMI CTAG 5 cut(s) 47, 194, 827, 1455, 1526
StyI CCWWGG 4 cut(s) 172, 181, 468, 1138
TaaI ACNGT 7 cut(s) 31, 226, 688, 883, 958, 1438, 1648
TaiI ACGT 5 cut(s) 429, 459, 650, 1323, 1419
TaqI TCGA 7 cut(s) 284, 453, 670, 894, 926, 935, 1634
TasI AATT 9 cut(s) 39, 72, 82, 106, 148, 362, 449, 1537, 1674
TatI WGTACW 1 cut(s) 387
TauI GCSGC 2 cut(s) 602, 1193
TfiI GAWTC 2 cut(s) 286, 1410
Tru1I TTAA 3 cut(s) 683, 1088, 1391
Tru9I TTAA 3 cut(s) 683, 1088, 1391
TscAI CASTG 2 cut(s) 405, 888
TseFI GTSAC 4 cut(s) 142, 883, 1432, 1450
TseI GCWGC 5 cut(s) 9, 380, 675, 818, 839
Tsp45I GTSAC 4 cut(s) 142, 883, 1432, 1450
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 2 cut(s) 405, 888
Vha464I CTTAAG 1 cut(s) 1390
VpaK11BI GGWCC 4 cut(s) 441, 463, 1513, 1691
XapI RAATTY 4 cut(s) 72, 82, 106, 1537
XceI RCATGY 1 cut(s) 942
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XhoI CTCGAG 2 cut(s) 669, 1633
XmiI GTMKAC 3 cut(s) 935, 960, 1317
XspI CTAG 5 cut(s) 47, 194, 827, 1455, 1526
Zsp2I ATGCAT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.