pycom09g04620

glucan endo-13-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0017090
Physical Location & Seq
Reverse (-)
3408263 .. 3409540
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g04620.1

Sequence Viewer

Length: 357 bp
ATGTCGAATTTTCTGATAAAGGTTTTAATTTTTCTGCTCGTTCTCTCAGCTGCTCCTCTCAAATCAGTTGCGGAGTTTGAGCAATGGTGCATCGCCGACGAGCAGACGCCGGACAGCGAGTTGCAGGCAGCAATGGATTGGGCTTGTGGGGGAGGAGGAGGTGCAGATTGTAGCAAGATTCAGGTGAACCAAGCTTGCTATTTCCCAAACACTGTGAAAGACCATGCTTCCTATGCATTCAACAGCTACTTTCAAAGATTCAAACACAAAGGTGGATCTTGCTACTTCAAGGGTGCTGCTTTAATTACAGAAATCGATCCAAGCCATGCCTCTTGCCACTATGAGTTTATTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

119

Amino Acids

13.01

Weight (kDa)

5.4

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 28 - 100 4.3e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35740
fragaria_vesca FvH4_6g41190
malus_domestica MD09G1121800.v1.1
prunus_persica Prupe.3G201800_v2.0.a1
pyrus_communis pycom09g04620
rosa_chinensis RchiOBHm_Chr2g0156751
rosa_laevigata RLG00000020937
rosa_multiflora Rmu_sc0008149.1_g000015
rosa_roxburghii Rroxscaffold_2G00092760
rosa_rugosa Rorug02G0456100
rosa_samantha Rh2AG521400 Rh2CG506300
rosa_wichuraiana Rw2G043040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 71
AclWI GGATC 2 cut(s) 283, 311
AcsI RAATTY 1 cut(s) 7
AcyI GRCGYC 1 cut(s) 107
AgsI TTSAA 4 cut(s) 241, 254, 262, 289
AleI CACNNNNGTG 1 cut(s) 270
AluBI AGCT 3 cut(s) 50, 194, 246
AluI AGCT 3 cut(s) 50, 194, 246
AlwI GGATC 2 cut(s) 283, 311
ApeKI GCWGC 3 cut(s) 50, 128, 296
ApoI RAATTY 1 cut(s) 7
AsuHPI GGTGA 1 cut(s) 196
BbvI GCAGC 3 cut(s) 37, 140, 283
BisI GCNGC 3 cut(s) 51, 129, 297
BlsI GCNGC 3 cut(s) 52, 130, 298
BmsI GCATC 1 cut(s) 99
Bsa29I ATCGAT 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 107
Bse3DI GCAATG 2 cut(s) 89, 138
BseCI ATCGAT 1 cut(s) 315
BseMI GCAATG 2 cut(s) 89, 138
BseMII CTCAG 1 cut(s) 60
BseRI GAGGAG 3 cut(s) 45, 168, 171
BseXI GCAGC 3 cut(s) 37, 140, 283
BsgI GTGCAG 1 cut(s) 183
BshVI ATCGAT 1 cut(s) 315
BsiSI CCGG 1 cut(s) 110
BsmI GAATGC 1 cut(s) 236
Bsp143I GATC 2 cut(s) 275, 316
BspACI CCGC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 59
BspDI ATCGAT 1 cut(s) 315
BspPI GGATC 2 cut(s) 283, 311
BsrDI GCAATG 2 cut(s) 89, 138
BssMI GATC 2 cut(s) 275, 316
BssNI GRCGYC 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 214
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 2 cut(s) 126, 196
BstDEI CTNAG 1 cut(s) 46
BstKTI GATC 2 cut(s) 278, 319
BstMBI GATC 2 cut(s) 275, 316
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstV1I GCAGC 3 cut(s) 37, 140, 283
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
Bsu15I ATCGAT 1 cut(s) 315
BsuTUI ATCGAT 1 cut(s) 315
BtgZI GCGATG 1 cut(s) 76
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 126, 196
ClaI ATCGAT 1 cut(s) 315
CseI GACGC 1 cut(s) 115
CviAII CATG 2 cut(s) 224, 326
CviJI RGCY 5 cut(s) 50, 143, 194, 246, 324
CviKI_1 RGCY 5 cut(s) 50, 143, 194, 246, 324
DdeI CTNAG 1 cut(s) 46
DpnI GATC 2 cut(s) 277, 318
DpnII GATC 2 cut(s) 275, 316
EcoT22I ATGCAT 1 cut(s) 238
FaeI CATG 2 cut(s) 227, 329
FaiI YATR 4 cut(s) 225, 234, 327, 342
FatI CATG 2 cut(s) 223, 325
Fnu4HI GCNGC 3 cut(s) 51, 129, 297
Fsp4HI GCNGC 3 cut(s) 51, 129, 297
GluI GCNGC 3 cut(s) 51, 129, 297
HapII CCGG 1 cut(s) 110
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 2 cut(s) 227, 329
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 2 cut(s) 178, 258
HpaII CCGG 1 cut(s) 110
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 1 cut(s) 15
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 1 cut(s) 101
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4V TGCA 4 cut(s) 90, 124, 164, 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 1 cut(s) 46
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 2 cut(s) 227, 329
Kzo9I GATC 2 cut(s) 275, 316
LmnI GCTCC 1 cut(s) 58
LpnPI CCDG 3 cut(s) 110, 123, 167
Lsp1109I GCAGC 3 cut(s) 37, 140, 283
LweI GCATC 1 cut(s) 99
MalI GATC 2 cut(s) 277, 318
MboI GATC 2 cut(s) 275, 316
MflI RGATCY 1 cut(s) 275
MluCI AATT 3 cut(s) 7, 27, 303
MnlI CCTC 5 cut(s) 66, 146, 149, 152, 340
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 2 cut(s) 26, 302
MslI CAYNNNNRTG 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 1 cut(s) 110
Mva1269I GAATGC 1 cut(s) 236
MwoI GCNNNNNNNGC 1 cut(s) 233
NdeII GATC 2 cut(s) 275, 316
NlaIII CATG 2 cut(s) 227, 329
NsiI ATGCAT 1 cut(s) 238
OliI CACNNNNGTG 1 cut(s) 270
PctI GAATGC 1 cut(s) 236
PfeI GAWTC 2 cut(s) 178, 258
PkrI GCNGC 3 cut(s) 52, 130, 298
PsuI RGATCY 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 50
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 2 cut(s) 26, 302
SatI GCNGC 3 cut(s) 51, 129, 297
Sau3AI GATC 2 cut(s) 275, 316
SetI ASST 7 cut(s) 24, 52, 163, 186, 196, 248, 274
SfaNI GCATC 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 270
Sse9I AATT 3 cut(s) 7, 27, 303
SsiI CCGC 1 cut(s) 71
TaaI ACNGT 1 cut(s) 214
TaqI TCGA 2 cut(s) 5, 315
TasI AATT 3 cut(s) 7, 27, 303
TfiI GAWTC 2 cut(s) 178, 258
Tru1I TTAA 2 cut(s) 26, 302
Tru9I TTAA 2 cut(s) 26, 302
TscAI CASTG 1 cut(s) 217
TseI GCWGC 3 cut(s) 50, 128, 296
TspRI CASTG 1 cut(s) 217
XapI RAATTY 1 cut(s) 7
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.