pycom09g10890

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006445
Physical Location & Seq
Forward (+)
8998148 .. 9000319
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1563 bp
ATGTCCAATCTAACTTCAGCATCAGGTGAAGCTGCTAGCGTCTCATCTGGCAACAGAAATGAAATTGGCACCAACTTTTCTCAGCAGTTTTTTACCACACCACCGCAAGCTCAACCCGCTCTCAAAAAGAAGAGAAATTTACCAGGCAATCCAGACCCAGATGCAGAAGTGATAGCCTTGTCCCCCAAGACACTCATGGCAACAAACAGGTTCATCTGTGAGATCTGCAACAAAGGGTTTCAGAGAGACCAGAATCTGCAGCTTCACAGAAGAGGGCACAACCTGCCATGGAAGCTCAAGCAAAGAACAAGCAAGGAGGTGAGGAAGAAGGTTTATGTGTGCCCAGAAGCCAGCTGTGTCCACCATGACCCTTCAAGGGCTCTTGGGGACCTGACTGGGATCAAGAAGCAATTTTGCAGAAAGCACGGTGAGAAGAAGTGGAAATGTGACAAGTGCTCAAAGAGGTATGCGGTTCAATCGGATTGGAAAGCTCATTCCAAAACCTGTGGCACTAGGGAGTACAGATGTGACTGTGGAACCCTATTCTCGAGGAGGGATAGTTTTATCACTCACAGAGCCTTCTGTGATGCTTTAGCAGAAGAGAGTGCAAGAGCCATCACCTCAGCAAACAACCCACATCATCCTCTCATCTCTCAGCAACAACAACAGATGAATCTTAACCAAGTGCAATTAGGGCACCAATTTAATCAAGACATCCATGGATTTTCACTGAAAAAAGAGCAGCAGAGTTTTACAACCCTAAGGCCAGATTTACCACCTTGGTTGGGCCCACCAAACTGCACCATTGACCTCTCCTCATCCTCCTCACTCTTCTCCCCAACCCACCACCAGGATTTATCCCTCGATGATTCCCATAATGGCACATCACAAAACCCTAACCCTAGCCCTAGCAGCTTGGGCCCCACACTCCCACCCTTCCACCCTGCACCTTCCCCTCACATGTCAGCCACTGCATTGCTACAGAAGGCAGCTCAGATGGGTGCAACCATGAGCAGCAAGCATAGTACTACTACAGCAGCAGCAGCAGCAGCTGCAGCCTCTGCATCACCACAGCCCATGGTGAGGGTCCACCAGCACAGCCAGGGTCACATGTCTGAGTTCAGTGGTCACGTGTCTGCTTTTGCAGCAGCAGGGGCCAATGCTACTAATAGCACAACTGGTCCTGCAGTCTCAGGTGTTCATCATCACCATCAAAACCAACATCATCAAGCGTCTCTACTCCATGATATGATGAACTCTCTGTCTTCCGGAACTGGGTTCGAAGGGGCTGCTTTTGAGCTGGAGGCTTTCGGCTCCCTCCCTAATATTTTGAACAATGCAAAAAAAGACTCGACCAATTCGACCCATTTCAACAGAAGTGGTAGCGACGACGGCGGTGCAAACGGTGAAGGTTTGACCCGAGATTTCTTGGGACTGAGAGCACTATCTCACAGTGACATTCTGAACATTGCTGGTATTGGAAACTGTGTCAGAAGTGCTAATGCTGCTGCTGCTGTTGCTAATACTGCTTCCCGTGACCACCAAAAACCTTGGCAAGGTTAA

Protein Analysis

521

Amino Acids

56.22

Weight (kDa)

9.13

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 108 - 142 3.2e-20 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 146 - 170 1.1e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 173 - 212 3.6e-24 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 291
AccB1I GGYRCC 2 cut(s) 68, 696
AccBSI CCGCTC 1 cut(s) 119
AccIII TCCGGA 1 cut(s) 1268
AciI CCGC 4 cut(s) 104, 117, 470, 1395
AclWI GGATC 1 cut(s) 407
AcsI RAATTY 1 cut(s) 136
AcvI CACGTG 1 cut(s) 1132
AfaI GTAC 2 cut(s) 521, 1027
AfiI CCNNNNNNNGG 6 cut(s) 376, 785, 850, 1083, 1275, 1556
AflIII ACRYGT 3 cut(s) 960, 1110, 1131
AgsI TTSAA 4 cut(s) 375, 476, 1333, 1372
AjnI CCWGG 3 cut(s) 142, 849, 1101
Alw21I GWGCWC 2 cut(s) 458, 1444
Alw26I GTCTC 4 cut(s) 46, 240, 1195, 1239
AlwI GGATC 1 cut(s) 407
AlwNI CAGNNNCTG 4 cut(s) 256, 971, 1052, 1061
Ama87I CYCGRG 2 cut(s) 547, 1419
Aor13HI TCCGGA 1 cut(s) 1268
AoxI GGCC 4 cut(s) 764, 787, 919, 1155
ApaI GGGCCC 2 cut(s) 791, 923
ApoI RAATTY 1 cut(s) 136
AspS9I GGNCC 8 cut(s) 388, 787, 788, 919, 920, 1087, 1155, 1181
AsuHPI GGTGA 8 cut(s) 38, 331, 440, 610, 1059, 1093, 1199, 1418
AsuII TTCGAA 1 cut(s) 1281
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 2 cut(s) 547, 1419
AvaII GGWCC 3 cut(s) 388, 1087, 1181
AxyI CCTNAGG 1 cut(s) 761
BaeGI GKGCMC 5 cut(s) 279, 344, 699, 791, 923
BanI GGYRCC 2 cut(s) 68, 696
BanII GRGCYC 3 cut(s) 382, 791, 923
BbrPI CACGTG 1 cut(s) 1132
BbsI GAAGAC 1 cut(s) 1257
Bbv12I GWGCWC 2 cut(s) 458, 1444
BbvCI CCTCAGC 1 cut(s) 622
BccI CCATC 3 cut(s) 623, 991, 1218
BceAI ACGGC 1 cut(s) 1408
BcgI CGANNNNNNTGC 4 cut(s) 1271, 1305, 1379, 1413
BciT130I CCWGG 3 cut(s) 144, 851, 1103
BcoDI GTCTC 4 cut(s) 46, 240, 1195, 1239
BfaI CTAG 4 cut(s) 36, 513, 903, 909
BfmI CTRYAG 5 cut(s) 257, 980, 1032, 1053, 1185
BfuAI ACCTGC 1 cut(s) 291
BglII AGATCT 1 cut(s) 222
BmcAI AGTACT 1 cut(s) 1027
Bme1390I CCNGG 3 cut(s) 144, 851, 1103
Bme18I GGWCC 3 cut(s) 388, 1087, 1181
BmeT110I CYCGRG 2 cut(s) 547, 1419
BmgT120I GGNCC 8 cut(s) 388, 787, 788, 919, 920, 1087, 1155, 1181
BmrFI CCNGG 3 cut(s) 144, 851, 1103
BmrI ACTGGG 2 cut(s) 405, 1284
BmsI GCATC 4 cut(s) 29, 151, 577, 1073
BmtI GCTAGC 1 cut(s) 39
BmuI ACTGGG 2 cut(s) 405, 1284
BpiI GAAGAC 1 cut(s) 1257
BpmI CTGGAG 1 cut(s) 1322
Bpu10I CCTNAGC 1 cut(s) 622
Bpu14I TTCGAA 1 cut(s) 1281
BpuEI CTTGAG 1 cut(s) 281
BsaAI YACGTR 1 cut(s) 1132
BsaI GGTCTC 1 cut(s) 240
BsaJI CCNNGG 6 cut(s) 287, 718, 779, 1077, 1102, 1550
BsaWI WCCGGW 1 cut(s) 1268
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 6 cut(s) 376, 785, 850, 1083, 1275, 1556
Bse1I ACTGG 3 cut(s) 400, 1183, 1279
Bse21I CCTNAGG 1 cut(s) 761
Bse3DI GCAATG 2 cut(s) 974, 1467
BseAI TCCGGA 1 cut(s) 1268
BseBI CCWGG 3 cut(s) 144, 851, 1103
BseDI CCNNGG 6 cut(s) 287, 718, 779, 1077, 1102, 1550
BseGI GGATG 3 cut(s) 640, 714, 818
BseLI CCNNNNNNNGG 6 cut(s) 376, 785, 850, 1083, 1275, 1556
BseMI GCAATG 2 cut(s) 974, 1467
BseMII CTCAG 7 cut(s) 95, 636, 668, 1007, 1107, 1206, 1427
BseNI ACTGG 3 cut(s) 400, 1183, 1279
BseRI GAGGAG 3 cut(s) 565, 805, 814
BseSI GKGCMC 5 cut(s) 279, 344, 699, 791, 923
BsgI GTGCAG 2 cut(s) 784, 930
BshFI GGCC 4 cut(s) 766, 789, 921, 1157
BshNI GGYRCC 2 cut(s) 68, 696
BsiHKAI GWGCWC 2 cut(s) 458, 1444
BsiHKCI CYCGRG 2 cut(s) 547, 1419
BsiSI CCGG 1 cut(s) 1269
BslFI GGGAC 3 cut(s) 166, 401, 1446
BslI CCNNNNNNNGG 6 cut(s) 376, 785, 850, 1083, 1275, 1556
BsmAI GTCTC 4 cut(s) 46, 240, 1195, 1239
BsmBI CGTCTC 2 cut(s) 46, 1239
BsmFI GGGAC 3 cut(s) 166, 401, 1446
BsnI GGCC 4 cut(s) 766, 789, 921, 1157
Bso31I GGTCTC 1 cut(s) 240
BsoBI CYCGRG 2 cut(s) 547, 1419
Bsp119I TTCGAA 1 cut(s) 1281
Bsp120I GGGCCC 2 cut(s) 787, 919
Bsp1286I GDGCHC 8 cut(s) 279, 344, 382, 458, 699, 791, 923, 1444
Bsp13I TCCGGA 1 cut(s) 1268
Bsp143I GATC 2 cut(s) 222, 399
Bsp19I CCATGG 3 cut(s) 287, 718, 1077
BspACI CCGC 4 cut(s) 104, 117, 470, 1395
BspANI GGCC 4 cut(s) 766, 789, 921, 1157
BspCNI CTCAG 7 cut(s) 94, 635, 667, 1006, 1108, 1205, 1428
BspEI TCCGGA 1 cut(s) 1268
BspMAI CTGCAG 3 cut(s) 261, 1057, 1189
BspMI ACCTGC 1 cut(s) 291
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 1 cut(s) 407
BspT104I TTCGAA 1 cut(s) 1281
BspT107I GGYRCC 2 cut(s) 68, 696
BspTNI GGTCTC 1 cut(s) 240
BsrBI CCGCTC 1 cut(s) 119
BsrDI GCAATG 2 cut(s) 974, 1467
BsrI ACTGG 3 cut(s) 400, 1183, 1279
BssECI CCNNGG 6 cut(s) 287, 718, 779, 1077, 1102, 1550
BssMI GATC 2 cut(s) 222, 399
BssT1I CCWWGG 5 cut(s) 287, 718, 779, 1077, 1550
Bst2UI CCWGG 3 cut(s) 144, 851, 1103
Bst4CI ACNGT 5 cut(s) 428, 533, 1406, 1454, 1487
Bst6I CTCTTC 4 cut(s) 125, 265, 594, 836
BstAPI GCANNNNNTGC 3 cut(s) 283, 1052, 1061
BstBAI YACGTR 1 cut(s) 1132
BstBI TTCGAA 1 cut(s) 1281
BstC8I GCNNGC 4 cut(s) 37, 108, 352, 1019
BstDEI CTNAG 8 cut(s) 81, 622, 654, 761, 993, 1116, 1192, 1436
BstDSI CCRYGG 3 cut(s) 287, 718, 1077
BstENI CCTNNNNNAGG 1 cut(s) 1554
BstF5I GGATG 3 cut(s) 640, 714, 818
BstKTI GATC 2 cut(s) 225, 402
BstMAI GTCTC 4 cut(s) 46, 240, 1195, 1239
BstMBI GATC 2 cut(s) 222, 399
BstNI CCWGG 3 cut(s) 144, 851, 1103
BstNSI RCATGY 2 cut(s) 964, 1114
BstSCI CCNGG 3 cut(s) 142, 849, 1101
BstSFI CTRYAG 5 cut(s) 257, 980, 1032, 1053, 1185
BstSLI GKGCMC 5 cut(s) 279, 344, 699, 791, 923
BstV2I GAAGAC 1 cut(s) 1257
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
Bsu36I CCTNAGG 1 cut(s) 761
BsuRI GGCC 4 cut(s) 766, 789, 921, 1157
BtgI CCRYGG 3 cut(s) 287, 718, 1077
BtsCI GGATG 3 cut(s) 640, 714, 818
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 4 cut(s) 728, 969, 1129, 1459
BveI ACCTGC 1 cut(s) 291
Cac8I GCNNGC 4 cut(s) 37, 108, 352, 1019
CaiI CAGNNNCTG 4 cut(s) 256, 971, 1052, 1061
Cfr13I GGNCC 8 cut(s) 388, 787, 788, 919, 920, 1087, 1155, 1181
CseI GACGC 2 cut(s) 28, 1221
Csp6I GTAC 2 cut(s) 520, 1026
CspCI CAANNNNNGTGG 6 cut(s) 487, 522, 765, 800, 1360, 1395
CviAII CATG 9 cut(s) 196, 288, 365, 719, 961, 1009, 1078, 1111, 1244
CviQI GTAC 2 cut(s) 520, 1026
DdeI CTNAG 8 cut(s) 81, 622, 654, 761, 993, 1116, 1192, 1436
DpnI GATC 2 cut(s) 224, 401
DpnII GATC 2 cut(s) 222, 399
Eam1104I CTCTTC 4 cut(s) 125, 265, 594, 836
EarI CTCTTC 4 cut(s) 125, 265, 594, 836
Eco130I CCWWGG 5 cut(s) 287, 718, 779, 1077, 1550
Eco24I GRGCYC 3 cut(s) 382, 791, 923
Eco31I GGTCTC 1 cut(s) 240
Eco47I GGWCC 3 cut(s) 388, 1087, 1181
Eco72I CACGTG 1 cut(s) 1132
Eco81I CCTNAGG 1 cut(s) 761
Eco88I CYCGRG 2 cut(s) 547, 1419
EcoNI CCTNNNNNAGG 1 cut(s) 1554
EcoO109I RGGNCCY 2 cut(s) 388, 920
EcoRII CCWGG 3 cut(s) 142, 849, 1101
EcoT14I CCWWGG 5 cut(s) 287, 718, 779, 1077, 1550
EcoT38I GRGCYC 3 cut(s) 382, 791, 923
ErhI CCWWGG 5 cut(s) 287, 718, 779, 1077, 1550
Esp3I CGTCTC 2 cut(s) 46, 1239
FaeI CATG 9 cut(s) 199, 291, 368, 722, 964, 1012, 1081, 1114, 1247
FaqI GGGAC 3 cut(s) 166, 401, 1446
FatI CATG 9 cut(s) 195, 287, 364, 718, 960, 1008, 1077, 1110, 1243
FauI CCCGC 1 cut(s) 124
FokI GGATG 3 cut(s) 627, 701, 805
FriOI GRGCYC 3 cut(s) 382, 791, 923
FspBI CTAG 4 cut(s) 36, 513, 903, 909
GsuI CTGGAG 1 cut(s) 1322
HaeIII GGCC 4 cut(s) 766, 789, 921, 1157
HapII CCGG 1 cut(s) 1269
HgaI GACGC 2 cut(s) 28, 1221
Hin1II CATG 9 cut(s) 199, 291, 368, 722, 964, 1012, 1081, 1114, 1247
HinfI GANTC 4 cut(s) 253, 673, 869, 1349
HpaII CCGG 1 cut(s) 1269
HphI GGTGA 8 cut(s) 38, 331, 440, 610, 1059, 1093, 1199, 1418
Hpy166II GTNNAC 2 cut(s) 361, 1090
Hpy188I TCNGA 6 cut(s) 243, 481, 996, 1117, 1464, 1493
Hpy188III TCNNGA 5 cut(s) 152, 403, 547, 710, 1269
Hpy8I GTNNAC 2 cut(s) 361, 1090
Hpy99I CGWCG 2 cut(s) 1391, 1394
HpyAV CCTTC 8 cut(s) 322, 381, 589, 946, 960, 979, 1277, 1403
HpyCH4III ACNGT 5 cut(s) 428, 533, 1406, 1454, 1487
HpyCH4IV ACGT 1 cut(s) 1131
HpyF3I CTNAG 8 cut(s) 81, 622, 654, 761, 993, 1116, 1192, 1436
HpySE526I ACGT 1 cut(s) 1131
Hsp92II CATG 9 cut(s) 199, 291, 368, 722, 964, 1012, 1081, 1114, 1247
Kpn2I TCCGGA 1 cut(s) 1268
Kzo9I GATC 2 cut(s) 222, 399
LmnI GCTCC 1 cut(s) 1319
LweI GCATC 4 cut(s) 29, 151, 577, 1073
MaeI CTAG 4 cut(s) 36, 513, 903, 909
MaeII ACGT 1 cut(s) 1131
MaeIII GTNAC 6 cut(s) 446, 527, 1106, 1127, 1454, 1535
MalI GATC 2 cut(s) 224, 401
MbiI CCGCTC 1 cut(s) 119
MboI GATC 2 cut(s) 222, 399
MboII GAAGA 7 cut(s) 142, 282, 337, 445, 611, 823, 1257
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 8 cut(s) 279, 344, 382, 458, 699, 791, 923, 1444
MluCI AATT 6 cut(s) 63, 136, 410, 689, 701, 1357
MlyI GAGTC 1 cut(s) 1343
MroI TCCGGA 1 cut(s) 1268
MseI TTAA 3 cut(s) 678, 705, 1561
MspA1I CMGCKG 2 cut(s) 354, 1052
MspI CCGG 1 cut(s) 1269
MspR9I CCNGG 3 cut(s) 144, 851, 1103
MvaI CCWGG 3 cut(s) 144, 851, 1103
NcoI CCATGG 3 cut(s) 287, 718, 1077
NdeII GATC 2 cut(s) 222, 399
NheI GCTAGC 1 cut(s) 35
NlaIII CATG 9 cut(s) 199, 291, 368, 722, 964, 1012, 1081, 1114, 1247
NmuCI GTSAC 6 cut(s) 446, 527, 1106, 1127, 1454, 1535
NspI RCATGY 2 cut(s) 964, 1114
NspV TTCGAA 1 cut(s) 1281
PaeR7I CTCGAG 1 cut(s) 547
PciI ACATGT 2 cut(s) 960, 1110
PcsI WCGNNNNNNNCGW 1 cut(s) 1358
PfeI GAWTC 3 cut(s) 253, 673, 869
PleI GAGTC 1 cut(s) 1343
PmaCI CACGTG 1 cut(s) 1132
PmlI CACGTG 1 cut(s) 1132
PpsI GAGTC 1 cut(s) 1343
Ppu21I YACGTR 1 cut(s) 1132
PpuMI RGGWCCY 1 cut(s) 388
PscI ACATGT 2 cut(s) 960, 1110
Psp5II RGGWCCY 1 cut(s) 388
Psp6I CCWGG 3 cut(s) 142, 849, 1101
PspCI CACGTG 1 cut(s) 1132
PspGI CCWGG 3 cut(s) 142, 849, 1101
PspOMI GGGCCC 2 cut(s) 787, 919
PspPI GGNCC 8 cut(s) 388, 787, 788, 919, 920, 1087, 1155, 1181
PspPPI RGGWCCY 1 cut(s) 388
PstI CTGCAG 3 cut(s) 261, 1057, 1189
PstNI CAGNNNCTG 4 cut(s) 256, 971, 1052, 1061
PsuI RGATCY 1 cut(s) 222
PvuII CAGCTG 2 cut(s) 354, 1052
RsaI GTAC 2 cut(s) 521, 1027
RsaNI GTAC 2 cut(s) 520, 1026
SaqAI TTAA 3 cut(s) 678, 705, 1561
Sau3AI GATC 2 cut(s) 222, 399
Sau96I GGNCC 8 cut(s) 388, 787, 788, 919, 920, 1087, 1155, 1181
ScaI AGTACT 1 cut(s) 1027
SchI GAGTC 1 cut(s) 1343
ScrFI CCNGG 3 cut(s) 144, 851, 1103
SduI GDGCHC 8 cut(s) 279, 344, 382, 458, 699, 791, 923, 1444
SfaNI GCATC 4 cut(s) 29, 151, 577, 1073
SfcI CTRYAG 5 cut(s) 257, 980, 1032, 1053, 1185
Sfr274I CTCGAG 1 cut(s) 547
SfuI TTCGAA 1 cut(s) 1281
SinI GGWCC 3 cut(s) 388, 1087, 1181
SlaI CTCGAG 1 cut(s) 547
SmlI CTYRAG 2 cut(s) 296, 547
SmoI CTYRAG 2 cut(s) 296, 547
Sse9I AATT 6 cut(s) 63, 136, 410, 689, 701, 1357
SsiI CCGC 4 cut(s) 104, 117, 470, 1395
SspI AATATT 1 cut(s) 1327
SspMI CTAG 4 cut(s) 36, 513, 903, 909
StyD4I CCNGG 3 cut(s) 142, 849, 1101
StyI CCWWGG 5 cut(s) 287, 718, 779, 1077, 1550
TaaI ACNGT 5 cut(s) 428, 533, 1406, 1454, 1487
TaiI ACGT 1 cut(s) 1134
TaqI TCGA 5 cut(s) 548, 864, 1281, 1352, 1361
TasI AATT 6 cut(s) 63, 136, 410, 689, 701, 1357
TatI WGTACW 2 cut(s) 519, 1025
TfiI GAWTC 3 cut(s) 253, 673, 869
Tru1I TTAA 3 cut(s) 678, 705, 1561
Tru9I TTAA 3 cut(s) 678, 705, 1561
TscAI CASTG 4 cut(s) 735, 976, 1129, 1459
TseFI GTSAC 6 cut(s) 446, 527, 1106, 1127, 1454, 1535
Tsp45I GTSAC 6 cut(s) 446, 527, 1106, 1127, 1454, 1535
TspDTI ATGAA 5 cut(s) 75, 202, 686, 1190, 1268
TspRI CASTG 4 cut(s) 735, 976, 1129, 1459
VpaK11BI GGWCC 3 cut(s) 388, 1087, 1181
XagI CCTNNNNNAGG 1 cut(s) 1554
XapI RAATTY 1 cut(s) 136
XceI RCATGY 2 cut(s) 964, 1114
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 547
XspI CTAG 4 cut(s) 36, 513, 903, 909
ZrmI AGTACT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.