pycom09g13180

Glycosyl-transferase family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006323
Physical Location & Seq
Forward (+)
11816751 .. 11820034
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2172 bp
ATGGAGGATAGCACTAGAGTGGAATATAAACCCTCGAGGGAAATTGGAAGTTTTAAGTCTATATTGTCAGGAAGATCGAGTCCTCGAAATTCTCCTTCATTTCGGAGATTGAATTCAAGCCGTACTCCACAAAAAGAAGGTAGAAGTAGTGGTGGTGTACAGTGGTTTCGGAGCAATCGGGTATTGTTTTGGTTGTTATTGATTACTCTTTGGGCTTATCTGGGAGTTTATTTTCAGTCCAGTTGGGCTCATAGCAATAACAAGGATAACTTCTTGGGGTTTCGAAATAAAGCAAGGAATGGGAACTCAGACAATGAACAGAATATGCAGAGAGATTTGCTTGCCAGCAACAGTTCTGTGGAAGTTAAGAATGAGACCGCCGAAAATCAGGTGGATGATGGTAAAAGGATACGTGTAGTTTCAACAAAAAAGGATTATGGAGTTTCATCTCATCGAAACGCAAGTCCAAAAAAGAGGAGTAAAAAAGGAGCTCGTAGTCTTCGTGGTAAAGGGCAAGGTAATCAGAAGAAAGCAGTGGAGGTTGATAGCCATGAGACAGAGGAGCAGGAATTGGACCTTCCTAAGACAAACACTACTTATGGGATGCTTGTTGGTCCATTTGGCGTTCTAGAGGATCGAATTTTAGAATGGAGTCCTAAAATGCGGTCTGGAACCTGTGACAGGAAGGGGGACTTTGCACGTCTTGTTTGGTCTAGGAGATTTATTTTAATATTCCATGAACTTTCAATGACTGGGGCTCCACTTTCTATGATGGGGTTAGCAACAGAGCTTTTGAGCTGTGGAGCCATGGTTTCTGCTGTAGTTCTTAGCAAGAAGGGTGGTTTGATGCCAGAGCTAGCTAGGAGAAGAATCAAGGTGCTTGAGGATAAAGAAAAACAAAGCTTCAAAACTGCTATGAAGGCAGATCTTGTCATCGCCGGGTCGGCAGTGTGTGCATCGTGGATTGATCAATACATGGATCATTTTCCAGCTGGTGCAAGTCAAATTGCTTGGTGGATCATGGAAAACCAGAGAGAATACTTCGATCACGCGAAGGTTGTGCTTAACCGAGTGAAAATGCTGGCTTTTCTATCAGAATCACAGTCTAAACAATGGCAAGACTGGTGTGAAGAAGAAAAGATTAACCTTAGGTCTCCACCTGCCGTTGTACCGCTCTCTATTAATGATGAGCTGGCCTTTGTAGCTGGCATAGCTTGTTCACTCAATACTCCATCTGCTAGTACTGAGAAGATGCTCGAGAAGAGACAGTTATTGCGAGATTCAGTCAGAAAGGAAATGGGGTTAACTGATAATGATATGCTTGTGATGACTCTGAGCAGTATAAACCCTGGAAAGGGGCAGCTCTTGCTTCTCGACTCAGCACGCTTGATGATTGAAGAACCTCTGAAAGATAATCCTAAGATAAAAAATCCAGTACGTAAGCGCCAAGCCCGCTCTACCTTGGGTAGAAAACATCACTTGAGAGCTTTGTTACAAGAATTGAAAGATGGTGTATCATCAAATGGATTACTACCGTCCAGGAAATCTGATGACCAGTTGAATGAACCCCAGAAGAATATCTTTCCATTAAGTAATCTCTATACCTCTGTTGATGGTACAGGTGCTTTGACTTTCGATGTCACTCATAAGAGGAAAGTGTTGTCAGATAAAGGAGGAACACTCAAACGGTCTGTTAAATTTCTTATTGGTTCTGTTGGATCTAAGAGTAATAAGGTCGTTTATGTCAAAGAACTTTTAGGATTCCTGTCTCAGCATTCAAACCTGTCAAAGTCAGTGTTGTGGACTCCAGCAACCACACGTGTAGCCGCATTATACTCTGCAGCTGACGTTTATGTAATGAACTCTCAGGGACTGGGAGAAACTTTTGGGAGAGTGACAATTGAAGCCATGGCATTCGGTCTTCCGGTGGTTGGAACAGAAGCTGGAGGCACAAAGGAGATTGTTGAACACAATGTAACAGGTCTGCTTCATCCTGTGGGGCAATCCGGGACCCATGGCCTCGCTGAAAATCTTCGGTTTTATCTTAAGAACCCAGTTTTAAGGAAGCAAATGGGACTGAAGGGACGAGAAAAGGTAGAGCGGATGTACCTAAAGCGGCACATGTACAAGAAATTTGTAGACGTTCTTCTCAAGTGCATGAGACCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016740 GO:0016757
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_trans_4_5 Glycos_transf_1

Protein Analysis

724

Amino Acids

81.06

Weight (kDa)

9.83

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_5 PF16994 237 - 406 3.3e-75 Glycosyl-transferase family 4
Glycos_transf_1 PF00534 589 - 697 3e-20 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 591 - 683 1.2e-17 Glycosyl transferases group 1
Glyco_trans_1_2 PF13524 605 - 712 6e-07 Glycosyl transferase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1168
AbsI CCTCGAGG 1 cut(s) 34
Acc36I ACCTGC 1 cut(s) 1168
AccBSI CCGCTC 3 cut(s) 1174, 1455, 2101
AccI GTMKAC 1 cut(s) 2139
AccII CGCG 1 cut(s) 1052
AciI CCGC 7 cut(s) 378, 664, 1172, 1453, 1827, 2101, 2116
AclWI GGATC 4 cut(s) 642, 987, 1025, 1726
AcsI RAATTY 5 cut(s) 88, 112, 639, 1697, 2132
AcuI CTGAAG 1 cut(s) 2099
AcvI CACGTG 1 cut(s) 1820
AfaI GTAC 8 cut(s) 124, 159, 1170, 1243, 1437, 1618, 2108, 2126
AfiI CCNNNNNNNGG 4 cut(s) 681, 1354, 1355, 1931
AflII CTTAAG 1 cut(s) 2045
AflIII ACRYGT 4 cut(s) 412, 1817, 1819, 2121
AjiI CACGTC 1 cut(s) 701
AjnI CCWGG 2 cut(s) 1348, 1538
AleI CACNNNNGTG 1 cut(s) 17
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 6 cut(s) 368, 548, 1158, 1258, 1773, 2155
AlwI GGATC 4 cut(s) 642, 987, 1025, 1726
AlwNI CAGNNNCTG 2 cut(s) 1873, 1943
Ama87I CYCGRG 2 cut(s) 34, 1256
AoxI GGCC 2 cut(s) 1194, 2017
ApeKI GCWGC 2 cut(s) 1360, 1841
ApoI RAATTY 5 cut(s) 88, 112, 639, 1697, 2132
ArsI GACNNNNNNTTYG 2 cut(s) 448, 480
AseI ATTAAT 1 cut(s) 1182
Asp700I GAANNNNTTC 1 cut(s) 2031
AspLEI GCGC 1 cut(s) 1446
AspS9I GGNCC 3 cut(s) 574, 614, 2010
AsuC2I CCSGG 2 cut(s) 940, 2008
AsuII TTCGAA 1 cut(s) 283
AsuNHI GCTAGC 1 cut(s) 856
AvaI CYCGRG 2 cut(s) 34, 1256
AvaII GGWCC 3 cut(s) 574, 614, 2010
AxyI CCTNAGG 1 cut(s) 1148
BanII GRGCYC 3 cut(s) 250, 493, 760
BarI GAAGNNNNNNTAC 2 cut(s) 2130, 2162
BbrPI CACGTG 1 cut(s) 1820
BbsI GAAGAC 2 cut(s) 491, 1913
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 2 cut(s) 1372, 1853
BccI CCATC 5 cut(s) 392, 766, 1240, 1502, 1607
BceAI ACGGC 2 cut(s) 105, 1148
BcgI CGANNNNNNTGC 2 cut(s) 1042, 1076
BciT130I CCWGG 2 cut(s) 1350, 1540
BciVI GTATCC 1 cut(s) 402
BclI TGATCA 1 cut(s) 967
BcnI CCSGG 2 cut(s) 940, 2008
BcoDI GTCTC 6 cut(s) 368, 548, 1158, 1258, 1773, 2155
BfaI CTAG 6 cut(s) 15, 629, 714, 857, 861, 1239
BfmI CTRYAG 2 cut(s) 819, 1839
BfoI RGCGCY 1 cut(s) 1447
BfrI CTTAAG 1 cut(s) 2045
BfuAI ACCTGC 1 cut(s) 1168
BfuI GTATCC 1 cut(s) 402
BglI GCCNNNNNGGC 1 cut(s) 944
BglII AGATCT 1 cut(s) 925
BisI GCNGC 4 cut(s) 1361, 1827, 1842, 2117
BlsI GCNGC 4 cut(s) 1362, 1828, 1843, 2118
BmcAI AGTACT 1 cut(s) 1243
Bme1390I CCNGG 4 cut(s) 940, 1350, 1540, 2008
Bme18I GGWCC 3 cut(s) 574, 614, 2010
BmeT110I CYCGRG 2 cut(s) 34, 1256
BmgBI CACGTC 1 cut(s) 701
BmgT120I GGNCC 3 cut(s) 574, 614, 2010
BmiI GGNNCC 5 cut(s) 673, 759, 805, 2011, 2012
BmrFI CCNGG 4 cut(s) 940, 1350, 1540, 2008
BmrI ACTGGG 3 cut(s) 762, 1883, 2048
BmsI GCATC 4 cut(s) 594, 837, 965, 1242
BmtI GCTAGC 1 cut(s) 860
BmuI ACTGGG 3 cut(s) 762, 1883, 2048
BpiI GAAGAC 2 cut(s) 491, 1913
BplI GAGNNNNNCTC 2 cut(s) 1665, 1697
BpmI CTGGAG 2 cut(s) 1791, 1965
Bpu14I TTCGAA 1 cut(s) 283
BpuEI CTTGAG 3 cut(s) 902, 1501, 2135
BpuMI CCSGG 2 cut(s) 940, 2008
BsaAI YACGTR 3 cut(s) 413, 1439, 1820
BsaI GGTCTC 3 cut(s) 368, 1158, 2155
BsaJI CCNNGG 5 cut(s) 807, 1348, 1461, 1908, 2014
BsaWI WCCGGW 1 cut(s) 1924
BsaXI ACNNNNNCTCC 4 cut(s) 742, 772, 795, 825
Bsc4I CCNNNNNNNGG 4 cut(s) 681, 1354, 1355, 1931
Bse1I ACTGG 7 cut(s) 240, 757, 1127, 1433, 1555, 1878, 2054
Bse21I CCTNAGG 1 cut(s) 1148
BseBI CCWGG 2 cut(s) 1350, 1540
BseDI CCNNGG 5 cut(s) 807, 1348, 1461, 1908, 2014
BseGI GGATG 4 cut(s) 400, 609, 1990, 2109
BseLI CCNNNNNNNGG 4 cut(s) 681, 1354, 1355, 1931
BseMII CTCAG 6 cut(s) 321, 1236, 1325, 1392, 1784, 1880
BseNI ACTGG 7 cut(s) 240, 757, 1127, 1433, 1555, 1878, 2054
BseRI GAGGAG 2 cut(s) 490, 575
BseXI GCAGC 2 cut(s) 1372, 1853
Bsh1236I CGCG 1 cut(s) 1052
BshFI GGCC 2 cut(s) 1196, 2019
BsiHKAI GWGCWC 1 cut(s) 493
BsiHKCI CYCGRG 2 cut(s) 34, 1256
BsiSI CCGG 3 cut(s) 939, 1925, 2007
BslFI GGGAC 5 cut(s) 704, 1884, 2023, 2088, 2097
BslI CCNNNNNNNGG 4 cut(s) 681, 1354, 1355, 1931
BsmAI GTCTC 6 cut(s) 368, 548, 1158, 1258, 1773, 2155
BsmFI GGGAC 5 cut(s) 704, 1884, 2023, 2088, 2097
BsmI GAATGC 2 cut(s) 1774, 1913
BsnI GGCC 2 cut(s) 1196, 2019
Bso31I GGTCTC 3 cut(s) 368, 1158, 2155
BsoBI CYCGRG 2 cut(s) 34, 1256
Bsp119I TTCGAA 1 cut(s) 283
Bsp1286I GDGCHC 3 cut(s) 250, 493, 760
Bsp1407I TGTACA 2 cut(s) 157, 2124
Bsp143I GATC 8 cut(s) 74, 634, 925, 967, 979, 1017, 1045, 1718
Bsp19I CCATGG 3 cut(s) 807, 1908, 2014
BspACI CCGC 7 cut(s) 378, 664, 1172, 1453, 1827, 2101, 2116
BspANI GGCC 2 cut(s) 1196, 2019
BspCNI CTCAG 6 cut(s) 320, 1237, 1326, 1391, 1783, 1879
BspFNI CGCG 1 cut(s) 1052
BspLI GGNNCC 5 cut(s) 673, 759, 805, 2011, 2012
BspMAI CTGCAG 1 cut(s) 1843
BspMI ACCTGC 1 cut(s) 1168
BspOI GCTAGC 1 cut(s) 860
BspPI GGATC 4 cut(s) 642, 987, 1025, 1726
BspT104I TTCGAA 1 cut(s) 283
BspTI CTTAAG 1 cut(s) 2045
BspTNI GGTCTC 3 cut(s) 368, 1158, 2155
BsrBI CCGCTC 3 cut(s) 1174, 1455, 2101
BsrGI TGTACA 2 cut(s) 157, 2124
BsrI ACTGG 7 cut(s) 240, 757, 1127, 1433, 1555, 1878, 2054
BssECI CCNNGG 5 cut(s) 807, 1348, 1461, 1908, 2014
BssMI GATC 8 cut(s) 74, 634, 925, 967, 979, 1017, 1045, 1718
BssT1I CCWWGG 4 cut(s) 807, 1461, 1908, 2014
Bst2UI CCWGG 2 cut(s) 1350, 1540
Bst4CI ACNGT 6 cut(s) 162, 353, 1104, 1269, 1536, 1689
Bst6I CTCTTC 1 cut(s) 1256
BstAFI CTTAAG 1 cut(s) 2045
BstAPI GCANNNNNTGC 2 cut(s) 953, 1366
BstAUI TGTACA 2 cut(s) 157, 2124
BstBAI YACGTR 3 cut(s) 413, 1439, 1820
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 8 cut(s) 342, 346, 858, 1083, 1194, 1207, 1384, 1453
BstDSI CCRYGG 3 cut(s) 807, 1908, 2014
BstENI CCTNNNNNAGG 1 cut(s) 679
BstF5I GGATG 4 cut(s) 400, 609, 1990, 2109
BstFNI CGCG 1 cut(s) 1052
BstH2I RGCGCY 1 cut(s) 1447
BstHHI GCGC 1 cut(s) 1446
BstKTI GATC 8 cut(s) 77, 637, 928, 970, 982, 1020, 1048, 1721
BstMAI GTCTC 6 cut(s) 368, 548, 1158, 1258, 1773, 2155
BstMBI GATC 8 cut(s) 74, 634, 925, 967, 979, 1017, 1045, 1718
BstMWI GCNNNNNNNGC 7 cut(s) 920, 944, 953, 1202, 1211, 1366, 1452
BstNI CCWGG 2 cut(s) 1350, 1540
BstNSI RCATGY 1 cut(s) 2125
BstSCI CCNGG 4 cut(s) 938, 1348, 1538, 2006
BstSFI CTRYAG 2 cut(s) 819, 1839
BstSNI TACGTA 1 cut(s) 1439
BstUI CGCG 1 cut(s) 1052
BstV1I GCAGC 2 cut(s) 1372, 1853
BstV2I GAAGAC 2 cut(s) 491, 1913
BstX2I RGATCY 2 cut(s) 925, 1718
BstYI RGATCY 2 cut(s) 925, 1718
Bsu36I CCTNAGG 1 cut(s) 1148
BsuI GTATCC 1 cut(s) 402
BsuRI GGCC 2 cut(s) 1196, 2019
BtgI CCRYGG 3 cut(s) 807, 1908, 2014
BtgZI GCGATG 1 cut(s) 919
BtrI CACGTC 1 cut(s) 701
BtsCI GGATG 4 cut(s) 400, 609, 1990, 2109
BtsI GCAGTG 2 cut(s) 540, 954
BtsIMutI CAGTG 4 cut(s) 167, 540, 954, 1800
BveI ACCTGC 1 cut(s) 1168
Cac8I GCNNGC 8 cut(s) 342, 346, 858, 1083, 1194, 1207, 1384, 1453
CaiI CAGNNNCTG 2 cut(s) 1873, 1943
CfoI GCGC 1 cut(s) 1446
Cfr13I GGNCC 3 cut(s) 574, 614, 2010
Csp6I GTAC 8 cut(s) 123, 158, 1169, 1242, 1436, 1617, 2107, 2125
CspCI CAANNNNNGTGG 2 cut(s) 820, 855
CviAII CATG 9 cut(s) 551, 737, 808, 976, 1021, 1909, 2015, 2122, 2158
CviQI GTAC 8 cut(s) 123, 158, 1169, 1242, 1436, 1617, 2107, 2125
DpnI GATC 8 cut(s) 76, 636, 927, 969, 981, 1019, 1047, 1720
DpnII GATC 8 cut(s) 74, 634, 925, 967, 979, 1017, 1045, 1718
Eam1104I CTCTTC 1 cut(s) 1256
EarI CTCTTC 1 cut(s) 1256
Ecl136II GAGCTC 1 cut(s) 491
Eco105I TACGTA 1 cut(s) 1439
Eco130I CCWWGG 4 cut(s) 807, 1461, 1908, 2014
Eco24I GRGCYC 3 cut(s) 250, 493, 760
Eco31I GGTCTC 3 cut(s) 368, 1158, 2155
Eco47I GGWCC 3 cut(s) 574, 614, 2010
Eco53kI GAGCTC 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 2099
Eco72I CACGTG 1 cut(s) 1820
Eco81I CCTNAGG 1 cut(s) 1148
Eco88I CYCGRG 2 cut(s) 34, 1256
EcoICRI GAGCTC 1 cut(s) 491
EcoNI CCTNNNNNAGG 1 cut(s) 679
EcoO109I RGGNCCY 1 cut(s) 2010
EcoRI GAATTC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 1348, 1538
EcoT14I CCWWGG 4 cut(s) 807, 1461, 1908, 2014
EcoT38I GRGCYC 3 cut(s) 250, 493, 760
ErhI CCWWGG 4 cut(s) 807, 1461, 1908, 2014
FaeI CATG 9 cut(s) 554, 740, 811, 979, 1024, 1912, 2018, 2125, 2161
FalI AAGNNNNNCTT 6 cut(s) 254, 286, 677, 709, 1565, 1597
FaqI GGGAC 5 cut(s) 704, 1884, 2023, 2088, 2097
FatI CATG 9 cut(s) 550, 736, 807, 975, 1020, 1908, 2014, 2121, 2157
FauI CCCGC 1 cut(s) 1460
FbaI TGATCA 1 cut(s) 967
FblI GTMKAC 1 cut(s) 2139
Fnu4HI GCNGC 4 cut(s) 1361, 1827, 1842, 2117
FokI GGATG 4 cut(s) 407, 616, 1977, 2116
FriOI GRGCYC 3 cut(s) 250, 493, 760
Fsp4HI GCNGC 4 cut(s) 1361, 1827, 1842, 2117
FspBI CTAG 6 cut(s) 15, 629, 714, 857, 861, 1239
GlaI GCGC 1 cut(s) 1445
GluI GCNGC 4 cut(s) 1361, 1827, 1842, 2117
GsuI CTGGAG 2 cut(s) 1791, 1965
HaeII RGCGCY 1 cut(s) 1447
HaeIII GGCC 2 cut(s) 1196, 2019
HapII CCGG 3 cut(s) 939, 1925, 2007
HhaI GCGC 1 cut(s) 1446
Hin1II CATG 9 cut(s) 554, 740, 811, 979, 1024, 1912, 2018, 2125, 2161
Hin6I GCGC 1 cut(s) 1444
HinP1I GCGC 1 cut(s) 1444
HincII GTYRAC 1 cut(s) 1305
HindII GTYRAC 1 cut(s) 1305
HindIII AAGCTT 1 cut(s) 901
HinfI GANTC 9 cut(s) 79, 652, 870, 1097, 1280, 1330, 1376, 1761, 1804
HpaI GTTAAC 1 cut(s) 1305
HpaII CCGG 3 cut(s) 939, 1925, 2007
Hpy166II GTNNAC 5 cut(s) 158, 1220, 1305, 1803, 2140
Hpy188III TCNNGA 5 cut(s) 69, 629, 669, 1258, 1373
Hpy8I GTNNAC 5 cut(s) 158, 1220, 1305, 1803, 2140
HpyAV CCTTC 8 cut(s) 105, 131, 587, 679, 829, 913, 1048, 2074
HpyCH4III ACNGT 6 cut(s) 162, 353, 1104, 1269, 1536, 1689
HpyCH4IV ACGT 6 cut(s) 412, 700, 1438, 1819, 1848, 2142
HpyCH4V TGCA 6 cut(s) 328, 698, 956, 998, 1841, 2157
HpyF10VI GCNNNNNNNGC 7 cut(s) 920, 944, 953, 1202, 1211, 1366, 1452
HpySE526I ACGT 6 cut(s) 412, 700, 1438, 1819, 1848, 2142
Hsp92II CATG 9 cut(s) 554, 740, 811, 979, 1024, 1912, 2018, 2125, 2161
HspAI GCGC 1 cut(s) 1444
KflI GGGWCCC 1 cut(s) 2010
Ksp22I TGATCA 1 cut(s) 967
KspAI GTTAAC 1 cut(s) 1305
Kzo9I GATC 8 cut(s) 74, 634, 925, 967, 979, 1017, 1045, 1718
LmnI GCTCC 5 cut(s) 171, 488, 562, 763, 803
Lsp1109I GCAGC 2 cut(s) 1372, 1853
LweI GCATC 4 cut(s) 594, 837, 965, 1242
MaeI CTAG 6 cut(s) 15, 629, 714, 857, 861, 1239
MaeII ACGT 6 cut(s) 412, 700, 1438, 1819, 1848, 2142
MaeIII GTNAC 5 cut(s) 677, 1491, 1639, 1894, 1975
MalI GATC 8 cut(s) 76, 636, 927, 969, 981, 1019, 1047, 1720
MbiI CCGCTC 3 cut(s) 1174, 1455, 2101
MboI GATC 8 cut(s) 74, 634, 925, 967, 979, 1017, 1045, 1718
MfeI CAATTG 1 cut(s) 1899
MflI RGATCY 2 cut(s) 925, 1718
MhlI GDGCHC 3 cut(s) 250, 493, 760
MlyI GAGTC 5 cut(s) 88, 661, 1324, 1370, 1798
MmeI TCCRAC 2 cut(s) 1696, 1912
MroXI GAANNNNTTC 1 cut(s) 2031
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 2 cut(s) 992, 1844
MspCI CTTAAG 1 cut(s) 2045
MspI CCGG 3 cut(s) 939, 1925, 2007
MspR9I CCNGG 4 cut(s) 940, 1350, 1540, 2008
MunI CAATTG 1 cut(s) 1899
Mva1269I GAATGC 2 cut(s) 1774, 1913
MvaI CCWGG 2 cut(s) 1350, 1540
MvnI CGCG 1 cut(s) 1052
MwoI GCNNNNNNNGC 7 cut(s) 920, 944, 953, 1202, 1211, 1366, 1452
NciI CCSGG 2 cut(s) 940, 2008
NcoI CCATGG 3 cut(s) 807, 1908, 2014
NdeII GATC 8 cut(s) 74, 634, 925, 967, 979, 1017, 1045, 1718
NheI GCTAGC 1 cut(s) 856
NlaIII CATG 9 cut(s) 554, 740, 811, 979, 1024, 1912, 2018, 2125, 2161
NlaIV GGNNCC 5 cut(s) 673, 759, 805, 2011, 2012
NmuCI GTSAC 3 cut(s) 677, 1639, 1894
NspI RCATGY 1 cut(s) 2125
NspV TTCGAA 1 cut(s) 283
OliI CACNNNNGTG 1 cut(s) 17
PaeR7I CTCGAG 2 cut(s) 34, 1256
PaqCI CACCTGC 1 cut(s) 1168
PciI ACATGT 1 cut(s) 2121
PcsI WCGNNNNNNNCGW 1 cut(s) 499
PctI GAATGC 2 cut(s) 1774, 1913
PdmI GAANNNNTTC 1 cut(s) 2031
PfeI GAWTC 4 cut(s) 870, 1097, 1280, 1761
PfoI TCCNGGA 2 cut(s) 1538, 2006
PkrI GCNGC 4 cut(s) 1362, 1828, 1843, 2118
PleI GAGTC 5 cut(s) 87, 660, 1324, 1370, 1798
PmaCI CACGTG 1 cut(s) 1820
PmlI CACGTG 1 cut(s) 1820
PpsI GAGTC 5 cut(s) 87, 660, 1324, 1370, 1798
Ppu21I YACGTR 3 cut(s) 413, 1439, 1820
PpuMI RGGWCCY 1 cut(s) 2010
PscI ACATGT 1 cut(s) 2121
PshBI ATTAAT 1 cut(s) 1182
Psp124BI GAGCTC 1 cut(s) 493
Psp5II RGGWCCY 1 cut(s) 2010
Psp6I CCWGG 2 cut(s) 1348, 1538
PspCI CACGTG 1 cut(s) 1820
PspGI CCWGG 2 cut(s) 1348, 1538
PspN4I GGNNCC 5 cut(s) 673, 759, 805, 2011, 2012
PspPI GGNCC 3 cut(s) 574, 614, 2010
PspPPI RGGWCCY 1 cut(s) 2010
PspXI VCTCGAGB 1 cut(s) 34
PstI CTGCAG 1 cut(s) 1843
PstNI CAGNNNCTG 2 cut(s) 1873, 1943
PsuI RGATCY 2 cut(s) 925, 1718
PvuII CAGCTG 2 cut(s) 992, 1844
RsaI GTAC 8 cut(s) 124, 159, 1170, 1243, 1437, 1618, 2108, 2126
RsaNI GTAC 8 cut(s) 123, 158, 1169, 1242, 1436, 1617, 2107, 2125
RseI CAYNNNNRTG 1 cut(s) 17
SacI GAGCTC 1 cut(s) 493
SatI GCNGC 4 cut(s) 1361, 1827, 1842, 2117
Sau3AI GATC 8 cut(s) 74, 634, 925, 967, 979, 1017, 1045, 1718
Sau96I GGNCC 3 cut(s) 574, 614, 2010
ScaI AGTACT 1 cut(s) 1243
SchI GAGTC 5 cut(s) 88, 661, 1324, 1370, 1798
ScrFI CCNGG 4 cut(s) 940, 1350, 1540, 2008
SduI GDGCHC 3 cut(s) 250, 493, 760
SfaNI GCATC 4 cut(s) 594, 837, 965, 1242
SfcI CTRYAG 2 cut(s) 819, 1839
Sfr274I CTCGAG 2 cut(s) 34, 1256
SfuI TTCGAA 1 cut(s) 283
SinI GGWCC 3 cut(s) 574, 614, 2010
SlaI CTCGAG 2 cut(s) 34, 1256
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 6 cut(s) 34, 881, 1256, 1480, 2045, 2150
SmoI CTYRAG 6 cut(s) 34, 881, 1256, 1480, 2045, 2150
SnaBI TACGTA 1 cut(s) 1439
SsiI CCGC 7 cut(s) 378, 664, 1172, 1453, 1827, 2101, 2116
SspI AATATT 1 cut(s) 732
SspMI CTAG 6 cut(s) 15, 629, 714, 857, 861, 1239
SstI GAGCTC 1 cut(s) 493
StyD4I CCNGG 4 cut(s) 938, 1348, 1538, 2006
StyI CCWWGG 4 cut(s) 807, 1461, 1908, 2014
TaaI ACNGT 6 cut(s) 162, 353, 1104, 1269, 1536, 1689
TaiI ACGT 6 cut(s) 415, 703, 1441, 1822, 1851, 2145
TaqII GACCGA 1 cut(s) 1907
TatI WGTACW 3 cut(s) 157, 1241, 2124
TauI GCSGC 2 cut(s) 1829, 2119
TfiI GAWTC 4 cut(s) 870, 1097, 1280, 1761
TscAI CASTG 4 cut(s) 167, 540, 954, 1800
TseFI GTSAC 3 cut(s) 677, 1639, 1894
TseI GCWGC 2 cut(s) 1360, 1841
Tsp45I GTSAC 3 cut(s) 677, 1639, 1894
TspDTI ATGAA 8 cut(s) 87, 330, 435, 753, 932, 1578, 1874, 1979
TspRI CASTG 4 cut(s) 167, 540, 954, 1800
Vha464I CTTAAG 1 cut(s) 2045
VpaK11BI GGWCC 3 cut(s) 574, 614, 2010
VspI ATTAAT 1 cut(s) 1182
XagI CCTNNNNNAGG 1 cut(s) 679
XapI RAATTY 5 cut(s) 88, 112, 639, 1697, 2132
XbaI TCTAGA 1 cut(s) 628
XceI RCATGY 1 cut(s) 2125
XhoI CTCGAG 2 cut(s) 34, 1256
XmiI GTMKAC 1 cut(s) 2139
XmnI GAANNNNTTC 1 cut(s) 2031
XspI CTAG 6 cut(s) 15, 629, 714, 857, 861, 1239
ZrmI AGTACT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.