pycom10g00130

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012571
Physical Location & Seq
Reverse (-)
182910 .. 184826
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00130.3

Sequence Viewer

Length: 654 bp
ATGAATTGTTGCAAGGTCATTAACAATCTGTTGCTGAATGCATCAATGTCAGAAAATCATGTATTAGCAGGAGCTGATGACTTCCTTCCTGTCCTTATATATGTCATGATCAAGGCCAATCCTCCACAATTGCATTCCAACCTCAGATTTATTCATTTATATCGAAGGCAGACAAAACTTGTGTCGGAAGCGGCTTATTACTTCACCAATCTAGTCTCAGCCAAAACATTTATTCTTGAGTTAAATGCTAAATCTCTTTCTATCGACGAAATCGAATTTGAAGAGAGTATGCATGCCGCAAGATTGACCAATAAAGCTACAGAAAAGGACGCCACACAAGCTTTGGAAGAAATGACAACCTCACAAGGCCAGACAGATCCTGGTTCATCAGCAGTGTTCCATTATAAAGAGAGAAGTGGTTCATCAAATTATCCTTATATGGACAAAGAGGCTGGAGATTTGACAGTTGGAGATGTCGAAAGACTGCTGAGTGCGTACAAGGAAGTGGTTACCAAGTATACCAGTCTGTGTACGGCTGTTAGAAACCTTTCTTTTTCAAGGGCTGAACCCCATGGCACTAATTTAGAAGCTAAAATTGTAGTGTCGACACAGGCGGAGGAAACAGGTAAGAATTCCGATCAAAGAGGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VPS9

Protein Analysis

218

Amino Acids

24.08

Weight (kDa)

5.71

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
AccI GTMKAC 2 cut(s) 518, 605
AciI CCGC 3 cut(s) 191, 297, 614
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 2 cut(s) 275, 631
AcyI GRCGYC 1 cut(s) 330
AfaI GTAC 2 cut(s) 497, 532
AgsI TTSAA 2 cut(s) 281, 558
AjnI CCWGG 1 cut(s) 379
AluBI AGCT 4 cut(s) 74, 317, 341, 590
AluI AGCT 4 cut(s) 74, 317, 341, 590
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 1 cut(s) 371
AlwNI CAGNNNCTG 2 cut(s) 74, 380
AoxI GGCC 2 cut(s) 114, 367
ApoI RAATTY 2 cut(s) 275, 631
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
Asp700I GAANNNNTTC 2 cut(s) 418, 547
AsuHPI GGTGA 1 cut(s) 196
BceAI ACGGC 1 cut(s) 549
BciT130I CCWGG 1 cut(s) 381
BclI TGATCA 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 220
BfaI CTAG 1 cut(s) 212
BfmI CTRYAG 1 cut(s) 318
BisI GCNGC 2 cut(s) 192, 297
BlsI GCNGC 2 cut(s) 193, 298
Bme1390I CCNGG 1 cut(s) 381
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 50
BpmI CTGGAG 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 571
Bse1I ACTGG 1 cut(s) 522
BseBI CCWGG 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 571
BseMII CTCAG 3 cut(s) 157, 231, 479
BseNI ACTGG 1 cut(s) 522
BshFI GGCC 2 cut(s) 116, 369
BsmAI GTCTC 1 cut(s) 220
BsmI GAATGC 2 cut(s) 43, 133
BsnI GGCC 2 cut(s) 116, 369
Bsp143I GATC 3 cut(s) 108, 376, 637
Bsp19I CCATGG 1 cut(s) 571
BspACI CCGC 3 cut(s) 191, 297, 614
BspANI GGCC 2 cut(s) 116, 369
BspCNI CTCAG 3 cut(s) 156, 230, 480
BspHI TCATGA 1 cut(s) 105
BspPI GGATC 1 cut(s) 371
BsrI ACTGG 1 cut(s) 522
BssECI CCNNGG 1 cut(s) 571
BssMI GATC 3 cut(s) 108, 376, 637
BssNAI GTATAC 1 cut(s) 519
BssNI GRCGYC 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 571
Bst1107I GTATAC 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 466
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 3 cut(s) 143, 217, 488
BstDSI CCRYGG 1 cut(s) 571
BstEII GGTNACC 1 cut(s) 508
BstKTI GATC 3 cut(s) 111, 379, 640
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 3 cut(s) 108, 376, 637
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 1 cut(s) 381
BstNSI RCATGY 1 cut(s) 296
BstPI GGTNACC 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 379
BstSFI CTRYAG 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BstZ17I GTATAC 1 cut(s) 519
BsuRI GGCC 2 cut(s) 116, 369
BtgI CCRYGG 1 cut(s) 571
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 1 cut(s) 294
CaiI CAGNNNCTG 2 cut(s) 74, 380
CciI TCATGA 1 cut(s) 105
CseI GACGC 1 cut(s) 338
Csp6I GTAC 2 cut(s) 496, 531
CviAII CATG 4 cut(s) 59, 106, 293, 572
CviQI GTAC 2 cut(s) 496, 531
DdeI CTNAG 3 cut(s) 143, 217, 488
DpnI GATC 3 cut(s) 110, 378, 639
DpnII GATC 3 cut(s) 108, 376, 637
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 571
Eco91I GGTNACC 1 cut(s) 508
EcoO65I GGTNACC 1 cut(s) 508
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 571
EcoT22I ATGCAT 2 cut(s) 43, 294
ErhI CCWWGG 1 cut(s) 571
FaeI CATG 4 cut(s) 62, 109, 296, 575
FatI CATG 4 cut(s) 58, 105, 292, 571
FbaI TGATCA 1 cut(s) 108
FblI GTMKAC 2 cut(s) 518, 605
Fnu4HI GCNGC 2 cut(s) 192, 297
Fsp4HI GCNGC 2 cut(s) 192, 297
FspBI CTAG 1 cut(s) 212
GluI GCNGC 2 cut(s) 192, 297
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 2 cut(s) 116, 369
HgaI GACGC 1 cut(s) 338
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 4 cut(s) 62, 109, 296, 575
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 339
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 3 cut(s) 519, 531, 606
Hpy188I TCNGA 4 cut(s) 52, 146, 187, 637
Hpy188III TCNNGA 2 cut(s) 106, 236
Hpy8I GTNNAC 3 cut(s) 519, 531, 606
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 95, 159
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 4 cut(s) 12, 41, 133, 292
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 3 cut(s) 143, 217, 488
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 4 cut(s) 62, 109, 296, 575
Ksp22I TGATCA 1 cut(s) 108
Kzo9I GATC 3 cut(s) 108, 376, 637
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 9 cut(s) 54, 102, 366, 383, 393, 438, 535, 596, 609
LweI GCATC 1 cut(s) 50
MaeI CTAG 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 508
MalI GATC 3 cut(s) 110, 378, 639
MboI GATC 3 cut(s) 108, 376, 637
MboII GAAGA 2 cut(s) 293, 359
MfeI CAATTG 1 cut(s) 128
MflI RGATCY 1 cut(s) 376
MluCI AATT 7 cut(s) 4, 128, 275, 427, 580, 594, 631
MmeI TCCRAC 3 cut(s) 162, 165, 448
MnlI CCTC 7 cut(s) 132, 152, 370, 442, 610, 638, 641
Mph1103I ATGCAT 2 cut(s) 43, 294
MroXI GAANNNNTTC 2 cut(s) 418, 547
MseI TTAA 2 cut(s) 21, 242
MspR9I CCNGG 1 cut(s) 381
MunI CAATTG 1 cut(s) 128
Mva1269I GAATGC 2 cut(s) 43, 133
MvaI CCWGG 1 cut(s) 381
MwoI GCNNNNNNNGC 1 cut(s) 338
NcoI CCATGG 1 cut(s) 571
NdeII GATC 3 cut(s) 108, 376, 637
NlaIII CATG 4 cut(s) 62, 109, 296, 575
NsiI ATGCAT 2 cut(s) 43, 294
NspI RCATGY 1 cut(s) 296
PaeI GCATGC 1 cut(s) 296
PagI TCATGA 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 2 cut(s) 43, 133
PdmI GAANNNNTTC 2 cut(s) 418, 547
PkrI GCNGC 2 cut(s) 193, 298
PsiI TTATAA 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 379
PspEI GGTNACC 1 cut(s) 508
PspGI CCWGG 1 cut(s) 379
PstNI CAGNNNCTG 2 cut(s) 74, 380
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 2 cut(s) 497, 532
RsaNI GTAC 2 cut(s) 496, 531
SalI GTCGAC 1 cut(s) 604
SaqAI TTAA 2 cut(s) 21, 242
SatI GCNGC 2 cut(s) 192, 297
Sau3AI GATC 3 cut(s) 108, 376, 637
ScrFI CCNGG 1 cut(s) 381
SetI ASST 9 cut(s) 18, 76, 144, 319, 343, 362, 549, 592, 628
SfaNI GCATC 1 cut(s) 50
SfcI CTRYAG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SphI GCATGC 1 cut(s) 296
Sse9I AATT 7 cut(s) 4, 128, 275, 427, 580, 594, 631
SsiI CCGC 3 cut(s) 191, 297, 614
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 379
StyI CCWWGG 1 cut(s) 571
TaaI ACNGT 1 cut(s) 466
TaqI TCGA 5 cut(s) 163, 264, 273, 477, 605
TasI AATT 7 cut(s) 4, 128, 275, 427, 580, 594, 631
TauI GCSGC 2 cut(s) 194, 299
Tru1I TTAA 2 cut(s) 21, 242
Tru9I TTAA 2 cut(s) 21, 242
TscAI CASTG 1 cut(s) 399
TspDTI ATGAA 4 cut(s) 17, 143, 375, 411
TspRI CASTG 1 cut(s) 399
XapI RAATTY 2 cut(s) 275, 631
XceI RCATGY 1 cut(s) 296
XcmI CCANNNNNNNNNTGG 2 cut(s) 340, 377
XmiI GTMKAC 2 cut(s) 518, 605
XmnI GAANNNNTTC 2 cut(s) 418, 547
XspI CTAG 1 cut(s) 212
Zsp2I ATGCAT 2 cut(s) 43, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.