Rroxscaffold_7G00217600

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012571
Physical Location & Seq
Forward (+)
68070540 .. 68074255
3716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217600.1

Sequence Viewer

Length: 891 bp
ATGGAGGGATCCTCCTCCTCCTCCTCCTCTTCCTATTCCTCGCCGTCTTCGGCCATCTTCTATGATTTTCTCGACCGAATGCGAAACCCTGCTTCCCTCGATCTCGTCCGGTCCATAAAAAGCTTTATTGTATCGTTTTCGTTTTACCCTGCCAACCCTGAAAATGATGGCAAAAGAGGTTTGGAGAAGTATGTCATGACGAAATTGTTCTCCCGGACCTTTTCTTCATCTCCTGAGGATGCAAAGATTGACCTTGAAGTCTCACAGAAAATACAGTTGTTGCAGACTTTCTTGAAGCCTGAGCATCTCGATATTCCGCTAGTTCTACGCAATGAAGCTTCATGGCTGGCCAATCCTCCACAATTACATTCCAACCTCAAATTTATCCAGTTATATCGAAGGCAGGCAAAACTTGTGTCAGAAGCGGCCTATTACCTCACCAATCTTGTCTCAGCTAAAACATTTATCTCTGAGTTAAATGCTAAATCTCTTTCGATTGATGAAATCGAATTTGAAGAGAATATGCAAGCAGCGAGACTGACCAATAAAGAGACACAAAAGGAGGCCACACCCACTCTGGAAGAAATGACAACCTCACAAGATCATGAAGATCCTGGCCCTTCATTGAGATCGCATGATAAAGAAACAAGGGGTGGGTCAAATTACCCCTATATGGACAAAGAAGCTGGGGATTTGACCGTTGGAGATGTGGAGAGGTTGCTGAGTGTGTACAAGGAAGTTGTTACCAAGTATACTAGCTTGTGCACCGCTTTTAGGCAGCTTTCATTATCTAGGATAGAAAACCATGTCACTAATTTAGAAGATAGAAACGTCTCATCAACACAATCGGCGGAAACAGGTATGAATTCAGGTCAAAGAGATGGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VPS9

Protein Analysis

296

Amino Acids

33.33

Weight (kDa)

5.24

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS9 PF02204 117 - 160 5.8e-09 Vacuolar sorting protein 9 (VPS9) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AccI GTMKAC 1 cut(s) 752
AciI CCGC 4 cut(s) 317, 425, 768, 851
AclWI GGATC 3 cut(s) 3, 16, 605
AcoI YGGCCR 2 cut(s) 51, 348
AcsI RAATTY 3 cut(s) 380, 509, 865
AfaI GTAC 1 cut(s) 731
AfiI CCNNNNNNNGG 2 cut(s) 673, 774
AgsI TTSAA 3 cut(s) 257, 295, 515
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 6 cut(s) 123, 338, 455, 686, 759, 781
AluI AGCT 6 cut(s) 123, 338, 455, 686, 759, 781
Alw21I GWGCWC 1 cut(s) 767
Alw26I GTCTC 5 cut(s) 265, 454, 529, 545, 838
Alw44I GTGCAC 1 cut(s) 763
AlwI GGATC 3 cut(s) 3, 16, 605
AoxI GGCC 5 cut(s) 51, 348, 426, 564, 616
ApaLI GTGCAC 1 cut(s) 763
ApeKI GCWGC 2 cut(s) 530, 778
ApoI RAATTY 3 cut(s) 380, 509, 865
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
Asp700I GAANNNNTTC 1 cut(s) 206
AspS9I GGNCC 3 cut(s) 111, 216, 617
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 1 cut(s) 430
AvaII GGWCC 2 cut(s) 111, 216
AxyI CCTNAGG 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 767
BalI TGGCCA 1 cut(s) 350
BamHI GGATCC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 767
BbvI GCAGC 2 cut(s) 542, 790
BccI CCATC 3 cut(s) 62, 161, 875
BceAI ACGGC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 615
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 5 cut(s) 265, 454, 529, 545, 838
BfaI CTAG 3 cut(s) 320, 756, 792
BisI GCNGC 3 cut(s) 426, 531, 779
BlsI GCNGC 3 cut(s) 427, 532, 780
Bme1390I CCNGG 2 cut(s) 214, 615
Bme18I GGWCC 2 cut(s) 111, 216
BmgT120I GGNCC 3 cut(s) 111, 216, 617
BmiI GGNNCC 1 cut(s) 10
BmrFI CCNGG 2 cut(s) 214, 615
BmsI GCATC 2 cut(s) 229, 313
BpiI GAAGAC 1 cut(s) 39
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 300
BpuMI CCSGG 1 cut(s) 214
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 2 cut(s) 673, 774
Bse1I ACTGG 1 cut(s) 388
Bse21I CCTNAGG 1 cut(s) 234
Bse3DI GCAATG 1 cut(s) 337
BseBI CCWGG 1 cut(s) 615
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 2 cut(s) 673, 774
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 5 cut(s) 225, 291, 462, 465, 713
BseNI ACTGG 1 cut(s) 388
BseRI GAGGAG 5 cut(s) 4, 7, 10, 13, 16
BseSI GKGCMC 1 cut(s) 767
BseXI GCAGC 2 cut(s) 542, 790
BseYI CCCAGC 1 cut(s) 686
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 5 cut(s) 53, 350, 428, 566, 618
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 767
BsiSI CCGG 2 cut(s) 109, 214
BslI CCNNNNNNNGG 2 cut(s) 673, 774
BsmAI GTCTC 5 cut(s) 265, 454, 529, 545, 838
BsmBI CGTCTC 1 cut(s) 838
BsmI GAATGC 1 cut(s) 84
BsnI GGCC 5 cut(s) 53, 350, 428, 566, 618
Bsp1286I GDGCHC 1 cut(s) 767
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 5 cut(s) 8, 100, 601, 610, 629
BspACI CCGC 4 cut(s) 317, 425, 768, 851
BspANI GGCC 5 cut(s) 53, 350, 428, 566, 618
BspCNI CTCAG 5 cut(s) 226, 292, 463, 464, 714
BspHI TCATGA 2 cut(s) 195, 604
BspLI GGNNCC 1 cut(s) 10
BspPI GGATC 3 cut(s) 3, 16, 605
BsrDI GCAATG 1 cut(s) 337
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 1 cut(s) 388
BssMI GATC 5 cut(s) 8, 100, 601, 610, 629
BssNAI GTATAC 1 cut(s) 753
Bst1107I GTATAC 1 cut(s) 753
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 2 cut(s) 276, 700
Bst6I CTCTTC 2 cut(s) 34, 510
BstAUI TGTACA 1 cut(s) 729
BstC8I GCNNGC 3 cut(s) 348, 405, 528
BstDEI CTNAG 5 cut(s) 234, 300, 451, 471, 722
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 5 cut(s) 11, 103, 604, 613, 632
BstMAI GTCTC 5 cut(s) 265, 454, 529, 545, 838
BstMBI GATC 5 cut(s) 8, 100, 601, 610, 629
BstMCI CGRYCG 1 cut(s) 76
BstNI CCWGG 1 cut(s) 615
BstSCI CCNGG 2 cut(s) 212, 613
BstSLI GKGCMC 1 cut(s) 767
BstV1I GCAGC 2 cut(s) 542, 790
BstV2I GAAGAC 1 cut(s) 39
BstX2I RGATCY 2 cut(s) 8, 610
BstYI RGATCY 2 cut(s) 8, 610
BstZ17I GTATAC 1 cut(s) 753
Bsu36I CCTNAGG 1 cut(s) 234
BsuRI GGCC 5 cut(s) 53, 350, 428, 566, 618
BtsCI GGATG 1 cut(s) 244
Cac8I GCNNGC 3 cut(s) 348, 405, 528
CciI TCATGA 2 cut(s) 195, 604
Cfr13I GGNCC 3 cut(s) 111, 216, 617
Csp6I GTAC 1 cut(s) 730
CviAII CATG 5 cut(s) 196, 342, 605, 635, 806
CviQI GTAC 1 cut(s) 730
DdeI CTNAG 5 cut(s) 234, 300, 451, 471, 722
DpnI GATC 5 cut(s) 10, 102, 603, 612, 631
DpnII GATC 5 cut(s) 8, 100, 601, 610, 629
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 2 cut(s) 51, 348
Eam1104I CTCTTC 2 cut(s) 34, 510
EarI CTCTTC 2 cut(s) 34, 510
EciI GGCGGA 1 cut(s) 866
Eco47I GGWCC 2 cut(s) 111, 216
Eco81I CCTNAGG 1 cut(s) 234
EcoRI GAATTC 1 cut(s) 865
EcoRII CCWGG 1 cut(s) 613
Esp3I CGTCTC 1 cut(s) 838
FaeI CATG 5 cut(s) 199, 345, 608, 638, 809
FatI CATG 5 cut(s) 195, 341, 604, 634, 805
FblI GTMKAC 1 cut(s) 752
Fnu4HI GCNGC 3 cut(s) 426, 531, 779
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 3 cut(s) 426, 531, 779
FspBI CTAG 3 cut(s) 320, 756, 792
GluI GCNGC 3 cut(s) 426, 531, 779
GsaI CCCAGC 1 cut(s) 690
HaeIII GGCC 5 cut(s) 53, 350, 428, 566, 618
HapII CCGG 2 cut(s) 109, 214
Hin1II CATG 5 cut(s) 199, 345, 608, 638, 809
HindIII AAGCTT 2 cut(s) 121, 336
HpaII CCGG 2 cut(s) 109, 214
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 3 cut(s) 730, 753, 765
Hpy188I TCNGA 2 cut(s) 421, 472
Hpy188III TCNNGA 7 cut(s) 71, 196, 233, 292, 308, 578, 605
Hpy8I GTNNAC 3 cut(s) 730, 753, 765
HpyAV CCTTC 2 cut(s) 393, 630
HpyCH4III ACNGT 2 cut(s) 276, 700
HpyCH4IV ACGT 1 cut(s) 831
HpyCH4V TGCA 4 cut(s) 242, 283, 526, 765
HpyF3I CTNAG 5 cut(s) 234, 300, 451, 471, 722
HpySE526I ACGT 1 cut(s) 831
Hsp92II CATG 5 cut(s) 199, 345, 608, 638, 809
Kzo9I GATC 5 cut(s) 8, 100, 601, 610, 629
Lsp1109I GCAGC 2 cut(s) 542, 790
LweI GCATC 2 cut(s) 229, 313
MaeI CTAG 3 cut(s) 320, 756, 792
MaeII ACGT 1 cut(s) 831
MaeIII GTNAC 2 cut(s) 742, 808
MalI GATC 5 cut(s) 10, 102, 603, 612, 631
MboI GATC 5 cut(s) 8, 100, 601, 610, 629
MboII GAAGA 8 cut(s) 21, 39, 49, 216, 527, 593, 620, 833
MflI RGATCY 2 cut(s) 8, 610
MhlI GDGCHC 1 cut(s) 767
MlsI TGGCCA 1 cut(s) 350
MluCI AATT 7 cut(s) 203, 362, 380, 509, 661, 814, 865
MluNI TGGCCA 1 cut(s) 350
MmeI TCCRAC 2 cut(s) 396, 682
Mox20I TGGCCA 1 cut(s) 350
MroXI GAANNNNTTC 1 cut(s) 206
MscI TGGCCA 1 cut(s) 350
MseI TTAA 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 350
MspI CCGG 2 cut(s) 109, 214
MspR9I CCNGG 2 cut(s) 214, 615
Mva1269I GAATGC 1 cut(s) 84
MvaI CCWGG 1 cut(s) 615
NciI CCSGG 1 cut(s) 214
NdeII GATC 5 cut(s) 8, 100, 601, 610, 629
NlaIII CATG 5 cut(s) 199, 345, 608, 638, 809
NlaIV GGNNCC 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 808
PagI TCATGA 2 cut(s) 195, 604
PctI GAATGC 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 206
PfoI TCCNGGA 1 cut(s) 212
PkrI GCNGC 3 cut(s) 427, 532, 780
Psp6I CCWGG 1 cut(s) 613
PspFI CCCAGC 1 cut(s) 686
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 1 cut(s) 10
PspPI GGNCC 3 cut(s) 111, 216, 617
PsuI RGATCY 2 cut(s) 8, 610
RsaI GTAC 1 cut(s) 731
RsaNI GTAC 1 cut(s) 730
SaqAI TTAA 1 cut(s) 476
SatI GCNGC 3 cut(s) 426, 531, 779
Sau3AI GATC 5 cut(s) 8, 100, 601, 610, 629
Sau96I GGNCC 3 cut(s) 111, 216, 617
ScrFI CCNGG 2 cut(s) 214, 615
SduI GDGCHC 1 cut(s) 767
SfaNI GCATC 2 cut(s) 229, 313
SinI GGWCC 2 cut(s) 111, 216
Sse9I AATT 7 cut(s) 203, 362, 380, 509, 661, 814, 865
SsiI CCGC 4 cut(s) 317, 425, 768, 851
SspMI CTAG 3 cut(s) 320, 756, 792
StyD4I CCNGG 2 cut(s) 212, 613
TaaI ACNGT 2 cut(s) 276, 700
TaiI ACGT 1 cut(s) 834
TaqI TCGA 6 cut(s) 72, 99, 309, 397, 494, 507
TaqII GACCGA 1 cut(s) 90
TasI AATT 7 cut(s) 203, 362, 380, 509, 661, 814, 865
TatI WGTACW 1 cut(s) 729
TauI GCSGC 1 cut(s) 428
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 2 cut(s) 530, 778
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 8 cut(s) 216, 330, 348, 516, 612, 621, 774, 878
VneI GTGCAC 1 cut(s) 763
VpaK11BI GGWCC 2 cut(s) 111, 216
XapI RAATTY 3 cut(s) 380, 509, 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmiI GTMKAC 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 3 cut(s) 320, 756, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.