pycom10g03660

Copper-transporting ATPase PAA1, chloroplastic-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0019595
Physical Location & Seq
Forward (+)
3969865 .. 3970831
967 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGGAGTCCACATTGTCCGCAGCGACGCCGACAACGGCAGCTCTCCTCGCCGTCTCCAAATCCCTCAACCGCCGACCGGAAAGGAGGACCTTTCTCAGTCAACGCCTCCCGAATCGTCTTCTTGCTCGCAGGCACCTCACTTCGGTTCACACCAACTACTACGGCTTTCAGGCCCCTAAACATGATCCTTTCTCTTCCTCGTCTCTGAGGAGTCTCTGCGTCGTTGGCCCGCTCTTACGGCGCCGTTTAGAGTGCGTTGGTAGCGGCGCTGCTTCTTTTGCTTCCGGTGGTGGAAATGGTGGCTTTGGTGGAGAGAGTTGCGGCAGCGGCGGTGACGGCGAGGGAGGCTCCGGCGGCGGCGACGCCAAGTCCAAGGTTGCTGCTGCTGGAGGCGATGAGGTTTCGGCGCTCTCCACTGATGTCATTGTTCTCGATGTTGGAGGAATGACATGTGGAGGATGTGCAGCCAGTGTGAAGCGAATTCTAGAAAATCAACCACAGGTATCGTCTGCGACTGTAAATCTCACAACAGAGACGGCAATTGTGTGTCCTGTGTCCGAAGCGAAGGCTACACCAAACTGGCAAAAGCAGTTAGGAGAGACACTTGCAAACCAATTGACCAGTTGCGGTTTCAAGTCTAACCTTCGTGGTAAGAATTTCACAATTGTTTCGCATTCAGCCTGTTACTTTTATTTATCGGTCTTATTAAACTGTGAATTCCATGGTTCTCAGTTAATGGAAAGTTGTATGTTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

253

Amino Acids

26.21

Weight (kDa)

8.71

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 143 - 212 2.9e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019595)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33520 AT4G33520
malus_domestica MD05G1044800.v1.1 MD10G1051800.v1.1
pyrus_communis pycom10g03660
rosa_chinensis RchiOBHm_Chr6g0271811
rosa_samantha Rh6CG181000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 132, 240
AccBSI CCGCTC 1 cut(s) 232
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 3 cut(s) 480, 655, 716
AcyI GRCGYC 3 cut(s) 26, 241, 363
AfiI CCNNNNNNNGG 2 cut(s) 76, 142
AflIII ACRYGT 1 cut(s) 449
AgsI TTSAA 1 cut(s) 634
AhdI GACNNNNNGTC 1 cut(s) 367
AluBI AGCT 1 cut(s) 41
AluI AGCT 1 cut(s) 41
Alw26I GTCTC 5 cut(s) 58, 207, 218, 527, 593
AlwI GGATC 1 cut(s) 179
AoxI GGCC 2 cut(s) 171, 226
ApeKI GCWGC 7 cut(s) 20, 38, 269, 324, 380, 383, 464
ApoI RAATTY 3 cut(s) 480, 655, 716
AspLEI GCGC 3 cut(s) 243, 269, 409
AspS9I GGNCC 3 cut(s) 87, 172, 227
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 1 cut(s) 87
BanI GGYRCC 2 cut(s) 132, 240
BbsI GAAGAC 1 cut(s) 110
BbvI GCAGC 7 cut(s) 32, 50, 256, 336, 367, 370, 476
BceAI ACGGC 7 cut(s) 35, 51, 178, 228, 254, 352, 552
BcoDI GTCTC 5 cut(s) 58, 207, 218, 527, 593
BfaI CTAG 1 cut(s) 485
BfoI RGCGCY 3 cut(s) 244, 270, 410
Bme18I GGWCC 1 cut(s) 87
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgT120I GGNCC 3 cut(s) 87, 172, 227
BmiI GGNNCC 4 cut(s) 134, 174, 242, 349
BpiI GAAGAC 1 cut(s) 110
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpmI CTGGAG 1 cut(s) 408
BsaHI GRCGYC 3 cut(s) 26, 241, 363
BsaJI CCNNGG 2 cut(s) 372, 721
BsaWI WCCGGW 2 cut(s) 76, 284
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 142
Bse1I ACTGG 3 cut(s) 468, 584, 621
BseDI CCNNGG 2 cut(s) 372, 721
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 2 cut(s) 76, 142
BseMII CTCAG 3 cut(s) 109, 197, 743
BseNI ACTGG 3 cut(s) 468, 584, 621
BseRI GAGGAG 2 cut(s) 35, 223
BseXI GCAGC 7 cut(s) 32, 50, 256, 336, 367, 370, 476
BsgI GTGCAG 1 cut(s) 483
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 173, 228
BshNI GGYRCC 2 cut(s) 132, 240
BsiEI CGRYCG 1 cut(s) 77
BsiSI CCGG 3 cut(s) 77, 285, 351
BslI CCNNNNNNNGG 2 cut(s) 76, 142
BsmAI GTCTC 5 cut(s) 58, 207, 218, 527, 593
BsmBI CGTCTC 3 cut(s) 58, 207, 527
BsmI GAATGC 1 cut(s) 673
BsnI GGCC 2 cut(s) 173, 228
Bsp143I GATC 1 cut(s) 184
Bsp19I CCATGG 1 cut(s) 721
BspANI GGCC 2 cut(s) 173, 228
BspCNI CTCAG 3 cut(s) 108, 198, 742
BspLI GGNNCC 4 cut(s) 134, 174, 242, 349
BspPI GGATC 1 cut(s) 179
BspT107I GGYRCC 2 cut(s) 132, 240
BsrBI CCGCTC 1 cut(s) 232
BsrI ACTGG 3 cut(s) 468, 584, 621
BssECI CCNNGG 2 cut(s) 372, 721
BssMI GATC 1 cut(s) 184
BssNI GRCGYC 3 cut(s) 26, 241, 363
BssT1I CCWWGG 2 cut(s) 372, 721
Bst4CI ACNGT 2 cut(s) 517, 713
Bst6I CTCTTC 1 cut(s) 199
BstACI GRCGYC 3 cut(s) 26, 241, 363
BstC8I GCNNGC 3 cut(s) 127, 131, 230
BstDEI CTNAG 3 cut(s) 95, 206, 729
BstDSI CCRYGG 1 cut(s) 721
BstF5I GGATG 1 cut(s) 464
BstH2I RGCGCY 3 cut(s) 244, 270, 410
BstHHI GCGC 3 cut(s) 243, 269, 409
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 5 cut(s) 58, 207, 218, 527, 593
BstMBI GATC 1 cut(s) 184
BstMCI CGRYCG 1 cut(s) 77
BstMWI GCNNNNNNNGC 9 cut(s) 47, 225, 238, 261, 278, 327, 336, 345, 354
BstNSI RCATGY 1 cut(s) 453
BstV1I GCAGC 7 cut(s) 32, 50, 256, 336, 367, 370, 476
BstV2I GAAGAC 1 cut(s) 110
BsuRI GGCC 2 cut(s) 173, 228
BtgI CCRYGG 1 cut(s) 721
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 1 cut(s) 464
BtsIMutI CAGTG 2 cut(s) 414, 475
Cac8I GCNNGC 3 cut(s) 127, 131, 230
CfoI GCGC 3 cut(s) 243, 269, 409
Cfr13I GGNCC 3 cut(s) 87, 172, 227
CseI GACGC 3 cut(s) 34, 208, 371
CviAII CATG 3 cut(s) 182, 450, 722
CviJI RGCY 9 cut(s) 41, 165, 173, 228, 303, 348, 467, 569, 680
CviKI_1 RGCY 9 cut(s) 41, 165, 173, 228, 303, 348, 467, 569, 680
DdeI CTNAG 3 cut(s) 95, 206, 729
DinI GGCGCC 1 cut(s) 242
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
DriI GACNNNNNGTC 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 199
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 372, 721
Eco47I GGWCC 1 cut(s) 87
EcoO109I RGGNCCY 2 cut(s) 87, 172
EcoRI GAATTC 2 cut(s) 480, 716
EcoT14I CCWWGG 2 cut(s) 372, 721
EgeI GGCGCC 1 cut(s) 242
EheI GGCGCC 1 cut(s) 242
ErhI CCWWGG 2 cut(s) 372, 721
Esp3I CGTCTC 3 cut(s) 58, 207, 527
FaeI CATG 3 cut(s) 185, 453, 725
FaiI YATR 4 cut(s) 183, 451, 723, 749
FatI CATG 3 cut(s) 181, 449, 721
FauI CCCGC 1 cut(s) 237
FokI GGATG 1 cut(s) 471
FspBI CTAG 1 cut(s) 485
GlaI GCGC 3 cut(s) 242, 268, 408
GsuI CTGGAG 1 cut(s) 408
HaeII RGCGCY 3 cut(s) 244, 270, 410
HaeIII GGCC 2 cut(s) 173, 228
HapII CCGG 3 cut(s) 77, 285, 351
HgaI GACGC 3 cut(s) 34, 208, 371
HhaI GCGC 3 cut(s) 243, 269, 409
Hin1I GRCGYC 3 cut(s) 26, 241, 363
Hin1II CATG 3 cut(s) 185, 453, 725
Hin6I GCGC 3 cut(s) 241, 267, 407
HinP1I GCGC 3 cut(s) 241, 267, 407
HincII GTYRAC 1 cut(s) 101
HindII GTYRAC 1 cut(s) 101
HinfI GANTC 3 cut(s) 5, 112, 211
HpaII CCGG 3 cut(s) 77, 285, 351
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 3 cut(s) 9, 101, 148
Hpy188I TCNGA 2 cut(s) 207, 559
Hpy188III TCNNGA 3 cut(s) 109, 431, 485
Hpy8I GTNNAC 3 cut(s) 9, 101, 148
Hpy99I CGWCG 3 cut(s) 28, 224, 365
HpyAV CCTTC 2 cut(s) 559, 653
HpyCH4III ACNGT 2 cut(s) 517, 713
HpyCH4V TGCA 2 cut(s) 464, 608
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 225, 238, 261, 278, 327, 336, 345, 354
HpyF3I CTNAG 3 cut(s) 95, 206, 729
Hsp92I GRCGYC 3 cut(s) 26, 241, 363
Hsp92II CATG 3 cut(s) 185, 453, 725
HspAI GCGC 3 cut(s) 241, 267, 407
KasI GGCGCC 1 cut(s) 240
Kzo9I GATC 1 cut(s) 184
LmnI GCTCC 1 cut(s) 353
Lsp1109I GCAGC 7 cut(s) 32, 50, 256, 336, 367, 370, 476
MaeI CTAG 1 cut(s) 485
MaeIII GTNAC 2 cut(s) 332, 683
MalI GATC 1 cut(s) 186
MbiI CCGCTC 1 cut(s) 232
MboI GATC 1 cut(s) 184
MboII GAAGA 2 cut(s) 110, 186
MfeI CAATTG 3 cut(s) 540, 614, 663
MluCI AATT 6 cut(s) 480, 540, 614, 655, 663, 716
Mly113I GGCGCC 1 cut(s) 241
MlyI GAGTC 2 cut(s) 14, 220
MmeI TCCRAC 1 cut(s) 418
MseI TTAA 3 cut(s) 707, 734, 757
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 3 cut(s) 77, 285, 351
MunI CAATTG 3 cut(s) 540, 614, 663
Mva1269I GAATGC 1 cut(s) 673
MwoI GCNNNNNNNGC 9 cut(s) 47, 225, 238, 261, 278, 327, 336, 345, 354
NarI GGCGCC 1 cut(s) 241
NcoI CCATGG 1 cut(s) 721
NdeII GATC 1 cut(s) 184
NlaIII CATG 3 cut(s) 185, 453, 725
NlaIV GGNNCC 4 cut(s) 134, 174, 242, 349
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 1 cut(s) 453
PciI ACATGT 1 cut(s) 449
PctI GAATGC 1 cut(s) 673
PfeI GAWTC 1 cut(s) 112
PleI GAGTC 2 cut(s) 13, 219
PluTI GGCGCC 1 cut(s) 244
PpsI GAGTC 2 cut(s) 13, 219
PpuMI RGGWCCY 1 cut(s) 87
PscI ACATGT 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 87
PspN4I GGNNCC 4 cut(s) 134, 174, 242, 349
PspPI GGNCC 3 cut(s) 87, 172, 227
PspPPI RGGWCCY 1 cut(s) 87
SaqAI TTAA 3 cut(s) 707, 734, 757
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 3 cut(s) 87, 172, 227
SchI GAGTC 2 cut(s) 14, 220
SetI ASST 7 cut(s) 43, 92, 138, 378, 402, 504, 645
SfoI GGCGCC 1 cut(s) 242
SinI GGWCC 1 cut(s) 87
Sse9I AATT 6 cut(s) 480, 540, 614, 655, 663, 716
SspDI GGCGCC 1 cut(s) 240
SspMI CTAG 1 cut(s) 485
StyI CCWWGG 2 cut(s) 372, 721
TaaI ACNGT 2 cut(s) 517, 713
TaqI TCGA 1 cut(s) 432
TaqII GACCGA 1 cut(s) 688
TasI AATT 6 cut(s) 480, 540, 614, 655, 663, 716
TauI GCSGC 5 cut(s) 267, 324, 330, 357, 360
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 3 cut(s) 707, 734, 757
Tru9I TTAA 3 cut(s) 707, 734, 757
TscAI CASTG 2 cut(s) 421, 475
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 7 cut(s) 20, 38, 269, 324, 380, 383, 464
Tsp45I GTSAC 1 cut(s) 332
TspRI CASTG 2 cut(s) 421, 475
VpaK11BI GGWCC 1 cut(s) 87
XapI RAATTY 3 cut(s) 480, 655, 716
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 1 cut(s) 453
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.