pycom10g08020

Belongs to the universal ribosomal protein uS9 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0018927
Physical Location & Seq
Forward (+)
10268965 .. 10269780
816 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
ATGGGATTACCTGGTCATAATCGAAACGGAGCTTCAATCCACCTGAATCGGTTGCTCACAACACATCCCTCTCTGTGCCTCTTTTTCCCTCTTGAACACACGAAGAATCCCTCAGCTCACCCTCTCTCTGTTACAACAACACAGTTCTATCGTAAGCTCGTCAGATCACGACCATTGGATTTTGTACTAAACTTTTTATTGGGTGCAGATTTGGGTGTGAGCTTAGCCTATCAGGCCCAACAACCCATCAGCCCATTAGGTCCACACAGAAATCACCTAAGGCCATTATATAAATACCCTCACTTACCCACACCACCACTTCCTGAATGTCGACTCTCGCAATCGAGTCAGTGCAGTGTTTTGGCCGCAAGAAGACCGCTGTCGCCGTCACATACTGCAAGCGCGGCTGTGGCCTCATTAAGATCAACGGCTGCCCAATCGAGCTCGTCGAGTCCGATCGATCCTCCGCTTCAAGGCCTACGAACCCATCCTACTCCTCGGACGACAGCGCTTCGCCGGTGTCGACATGCGCATCCGCGTCAAGGGAGGCGGTCACACCTCCCAGATCTACGCCATCCGCCAGAGCATCGCCAAAGCTCTCGTCGCTTTTTACCAGAAATATGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S9

Protein Analysis

210

Amino Acids

23.23

Weight (kDa)

11.47

Isoelectric Point (pI)

76.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018927)

Species Orthologous Gene IDs
pyrus_communis pycom10g08020
rosa_chinensis RchiOBHm_Chr6g0255201
rosa_multiflora Rmu_sc0001879.1_g000005
rosa_roxburghii Rroxscaffold_1G00030090
rosa_rugosa Rorug02G0279800
rosa_samantha Rh3BG175600 Rh3CG166700 Rh4AG078700 Rh4DG213700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 531
AccI GTMKAC 2 cut(s) 331, 523
AccII CGCG 2 cut(s) 404, 538
AciI CCGC 7 cut(s) 366, 377, 404, 467, 536, 550, 578
AclWI GGATC 1 cut(s) 455
AcoI YGGCCR 1 cut(s) 363
AfaI GTAC 1 cut(s) 186
AfeI AGCGCT 1 cut(s) 510
AfiI CCNNNNNNNGG 2 cut(s) 473, 542
AgsI TTSAA 3 cut(s) 36, 95, 473
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 6 cut(s) 32, 116, 157, 222, 444, 597
AluI AGCT 6 cut(s) 32, 116, 157, 222, 444, 597
Alw21I GWGCWC 1 cut(s) 446
AlwI GGATC 1 cut(s) 455
Aor51HI AGCGCT 1 cut(s) 510
AoxI GGCC 5 cut(s) 234, 281, 363, 411, 475
ApeKI GCWGC 1 cut(s) 431
ArsI GACNNNNNNTTYG 2 cut(s) 586, 618
AspLEI GCGC 3 cut(s) 404, 511, 532
AspS9I GGNCC 2 cut(s) 235, 260
AsuHPI GGTGA 2 cut(s) 110, 266
AvaII GGWCC 1 cut(s) 260
AxyI CCTNAGG 1 cut(s) 278
BanII GRGCYC 1 cut(s) 446
BbsI GAAGAC 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 446
BbvCI CCTCAGC 1 cut(s) 112
BbvI GCAGC 1 cut(s) 418
BccI CCATC 3 cut(s) 254, 495, 582
BceAI ACGGC 2 cut(s) 370, 444
BciT130I CCWGG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 512
BglI GCCNNNNNGGC 1 cut(s) 233
BglII AGATCT 1 cut(s) 565
BisI GCNGC 3 cut(s) 366, 405, 432
BlpI GCTNAGC 1 cut(s) 223
BlsI GCNGC 3 cut(s) 367, 406, 433
Bme1390I CCNGG 1 cut(s) 12
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 235, 260
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 2 cut(s) 541, 595
BoxI GACNNNNGTC 1 cut(s) 379
BpiI GAAGAC 1 cut(s) 379
Bpu10I CCTNAGC 1 cut(s) 112
Bpu1102I GCTNAGC 1 cut(s) 223
Bsa29I ATCGAT 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 497
Bsc4I CCNNNNNNNGG 2 cut(s) 473, 542
Bse118I RCCGGY 1 cut(s) 516
Bse21I CCTNAGG 1 cut(s) 278
BseBI CCWGG 1 cut(s) 12
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 497
BseGI GGATG 4 cut(s) 64, 487, 532, 574
BseLI CCNNNNNNNGG 2 cut(s) 473, 542
BseMII CTCAG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 486
BseXI GCAGC 1 cut(s) 418
BsgI GTGCAG 2 cut(s) 225, 373
Bsh1236I CGCG 2 cut(s) 404, 538
Bsh1285I CGRYCG 1 cut(s) 459
BshFI GGCC 5 cut(s) 236, 283, 365, 413, 477
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 446
BsiSI CCGG 1 cut(s) 517
BslI CCNNNNNNNGG 2 cut(s) 473, 542
BsnI GGCC 5 cut(s) 236, 283, 365, 413, 477
Bsp1286I GDGCHC 1 cut(s) 446
Bsp143I GATC 5 cut(s) 164, 422, 456, 460, 565
Bsp1720I GCTNAGC 1 cut(s) 223
BspACI CCGC 7 cut(s) 366, 377, 404, 467, 536, 550, 578
BspANI GGCC 5 cut(s) 236, 283, 365, 413, 477
BspCNI CTCAG 1 cut(s) 125
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 2 cut(s) 404, 538
BspPI GGATC 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 516
BssAI RCCGGY 1 cut(s) 516
BssECI CCNNGG 1 cut(s) 497
BssMI GATC 5 cut(s) 164, 422, 456, 460, 565
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 3 cut(s) 112, 223, 278
BstF5I GGATG 4 cut(s) 64, 487, 532, 574
BstFNI CGCG 2 cut(s) 404, 538
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 3 cut(s) 404, 511, 532
BstKTI GATC 5 cut(s) 167, 425, 459, 463, 568
BstMBI GATC 5 cut(s) 164, 422, 456, 460, 565
BstMCI CGRYCG 1 cut(s) 459
BstMWI GCNNNNNNNGC 4 cut(s) 233, 404, 410, 603
BstNI CCWGG 1 cut(s) 12
BstNSI RCATGY 1 cut(s) 530
BstPAI GACNNNNGTC 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 10
BstUI CGCG 2 cut(s) 404, 538
BstV1I GCAGC 1 cut(s) 418
BstV2I GAAGAC 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
Bsu15I ATCGAT 1 cut(s) 459
Bsu36I CCTNAGG 1 cut(s) 278
BsuRI GGCC 5 cut(s) 236, 283, 365, 413, 477
BsuTUI ATCGAT 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 572
BtsCI GGATG 4 cut(s) 64, 487, 532, 574
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 2 cut(s) 356, 361
Cac8I GCNNGC 1 cut(s) 400
CfoI GCGC 3 cut(s) 404, 511, 532
Cfr10I RCCGGY 1 cut(s) 516
Cfr13I GGNCC 2 cut(s) 235, 260
ClaI ATCGAT 1 cut(s) 459
CseI GACGC 1 cut(s) 527
CsiI ACCWGGT 1 cut(s) 10
Csp6I GTAC 1 cut(s) 185
CviAII CATG 1 cut(s) 527
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 3 cut(s) 112, 223, 278
DpnI GATC 5 cut(s) 166, 424, 458, 462, 567
DpnII GATC 5 cut(s) 164, 422, 456, 460, 565
EaeI YGGCCR 1 cut(s) 363
EciI GGCGGA 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 444
Eco147I AGGCCT 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 260
Eco47III AGCGCT 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 444
Eco81I CCTNAGG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 444
EcoRII CCWGG 1 cut(s) 10
EcoT38I GRGCYC 1 cut(s) 446
FaeI CATG 1 cut(s) 530
FaiI YATR 6 cut(s) 18, 289, 291, 393, 528, 622
FatI CATG 1 cut(s) 526
FblI GTMKAC 2 cut(s) 331, 523
Fnu4HI GCNGC 3 cut(s) 366, 405, 432
FokI GGATG 4 cut(s) 51, 474, 519, 561
FriOI GRGCYC 1 cut(s) 446
Fsp4HI GCNGC 3 cut(s) 366, 405, 432
FspAI RTGCGCAY 1 cut(s) 531
FspI TGCGCA 1 cut(s) 531
GlaI GCGC 3 cut(s) 403, 510, 531
GluI GCNGC 3 cut(s) 366, 405, 432
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 5 cut(s) 236, 283, 365, 413, 477
HapII CCGG 1 cut(s) 517
HgaI GACGC 1 cut(s) 527
HhaI GCGC 3 cut(s) 404, 511, 532
Hin1II CATG 1 cut(s) 530
Hin6I GCGC 3 cut(s) 402, 509, 530
HinP1I GCGC 3 cut(s) 402, 509, 530
HincII GTYRAC 2 cut(s) 332, 524
HindII GTYRAC 2 cut(s) 332, 524
HinfI GANTC 5 cut(s) 46, 106, 333, 346, 451
HpaII CCGG 1 cut(s) 517
HphI GGTGA 2 cut(s) 110, 266
Hpy166II GTNNAC 3 cut(s) 263, 332, 524
Hpy188I TCNGA 3 cut(s) 164, 456, 501
Hpy188III TCNNGA 3 cut(s) 92, 168, 323
Hpy8I GTNNAC 3 cut(s) 263, 332, 524
Hpy99I CGWCG 2 cut(s) 451, 606
HpyCH4III ACNGT 1 cut(s) 144
HpyCH4V TGCA 3 cut(s) 206, 354, 398
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 404, 410, 603
HpyF3I CTNAG 3 cut(s) 112, 223, 278
Hsp92II CATG 1 cut(s) 530
HspAI GCGC 3 cut(s) 402, 509, 530
Kzo9I GATC 5 cut(s) 164, 422, 456, 460, 565
LmnI GCTCC 1 cut(s) 29
LpnPI CCDG 7 cut(s) 24, 56, 218, 336, 530, 576, 594
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 2 cut(s) 541, 595
MabI ACCWGGT 1 cut(s) 10
MaeIII GTNAC 3 cut(s) 130, 387, 552
MalI GATC 5 cut(s) 166, 424, 458, 462, 567
MboI GATC 5 cut(s) 164, 422, 456, 460, 565
MboII GAAGA 2 cut(s) 115, 384
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 1 cut(s) 446
MlyI GAGTC 3 cut(s) 327, 355, 460
MseI TTAA 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 379
MspI CCGG 1 cut(s) 517
MspR9I CCNGG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 12
MvnI CGCG 2 cut(s) 404, 538
MwoI GCNNNNNNNGC 4 cut(s) 233, 404, 410, 603
NdeII GATC 5 cut(s) 164, 422, 456, 460, 565
NlaIII CATG 1 cut(s) 530
NmuCI GTSAC 2 cut(s) 387, 552
NsbI TGCGCA 1 cut(s) 531
NspI RCATGY 1 cut(s) 530
PceI AGGCCT 1 cut(s) 477
PcsI WCGNNNNNNNCGW 3 cut(s) 446, 452, 520
PfeI GAWTC 2 cut(s) 46, 106
PkrI GCNGC 3 cut(s) 367, 406, 433
Ple19I CGATCG 1 cut(s) 459
PleI GAGTC 3 cut(s) 327, 354, 459
PpsI GAGTC 3 cut(s) 327, 354, 459
PshAI GACNNNNGTC 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspPI GGNCC 2 cut(s) 235, 260
PsrI GAACNNNNNNTAC 2 cut(s) 475, 507
PsuI RGATCY 1 cut(s) 565
PvuI CGATCG 1 cut(s) 459
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
SacI GAGCTC 1 cut(s) 446
SalI GTCGAC 2 cut(s) 330, 522
SaqAI TTAA 1 cut(s) 419
SatI GCNGC 3 cut(s) 366, 405, 432
Sau3AI GATC 5 cut(s) 164, 422, 456, 460, 565
Sau96I GGNCC 2 cut(s) 235, 260
SchI GAGTC 3 cut(s) 327, 355, 460
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 1 cut(s) 446
SexAI ACCWGGT 1 cut(s) 10
SfaNI GCATC 2 cut(s) 541, 595
SgrAI CRCCGGYG 1 cut(s) 516
SinI GGWCC 1 cut(s) 260
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 7 cut(s) 366, 377, 404, 467, 536, 550, 578
SstI GAGCTC 1 cut(s) 446
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 10
TaaI ACNGT 1 cut(s) 144
TaqI TCGA 7 cut(s) 22, 331, 344, 440, 449, 459, 523
TatI WGTACW 1 cut(s) 184
TauI GCSGC 2 cut(s) 368, 407
TfiI GAWTC 2 cut(s) 46, 106
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TscAI CASTG 2 cut(s) 356, 361
TseFI GTSAC 2 cut(s) 387, 552
TseI GCWGC 1 cut(s) 431
Tsp45I GTSAC 2 cut(s) 387, 552
TspGWI ACGGA 1 cut(s) 42
TspRI CASTG 2 cut(s) 356, 361
VpaK11BI GGWCC 1 cut(s) 260
XceI RCATGY 1 cut(s) 530
XmiI GTMKAC 2 cut(s) 331, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.