pycom10g15190

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0018139
Physical Location & Seq
Forward (+)
18496546 .. 18500247
3702 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
ATGAACATGATCTCATTTAAAGCCAGAGTATATGAGCAAAACGATTCTGTAGCAGTAGTGGGTTGCGGACTCGGTGGAGGTTCACTAGTGAATGCAGGAGTTATGATGCCGACACCAGTTCGAGCCAGACGGCATCCAAAATGGCCAAAGCAATGGGAAAAGAATTGGGATAGTTGTGAAGCTTCTGCAGCTGCCATGCTGAAAGTACAAAGCCTTCCTGACAAGTTTTCTGCTGCGAAAGTCTTGGAAGATCATATCACTAGTGAAGAAACTTTTGAGACTCCGGTGAGGCTGACTATGAATTTTGACTTTGAAGATGGTCAACGTCCACTATCCAATAATAATCCAATTAAGAAGGTTCAACAGATGAGTAGCTGCTTAGCCTGTGGAAATTGTCTCTCTGGATGTCCTTATAATGCCAAAATTTCTAACGACAAGACTTATCTACTTTCAGCAATCCAGATCCTCTTTCAGTCACAAATGAGAGGACTAAGAGTTTCAGAAACACTTGGTTCAGGATTCAGCTGCAACGGAAATACTGTTGCCTATCTCGCCGGAAGCCCGATGCCGTTGAATGGTTATGGACTAGATAAGAAGCAACTCTTTAAGATTCCATTTCAAGCAAGGCCAGGACCATCCATCTCATCATCGTACACTTCTTCGTTGGGGTTTACAATTCAAGTAAGAGAACATTATCTTATAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

236

Amino Acids

25.82

Weight (kDa)

8.95

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 17 - 154 5.2e-06 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018139)

Species Orthologous Gene IDs
pyrus_communis pycom10g15180 pycom10g15190
rosa_chinensis RchiOBHm_Chr3g0474671
rosa_multiflora Rmu_sc0001353.1_g000047 Rmu_sc0002324.1_g000028
rosa_roxburghii Rroxscaffold_6G00407000
rosa_rugosa Rorug03G0142000
rosa_samantha Rh3AG192800 Rh3BG222700 Rh3CG217400 Rh3DG218300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 414, 701
AciI CCGC 1 cut(s) 66
AclWI GGATC 1 cut(s) 457
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 2 cut(s) 301, 423
AfaI GTAC 2 cut(s) 207, 653
AfiI CCNNNNNNNGG 1 cut(s) 575
AgsI TTSAA 5 cut(s) 314, 362, 574, 620, 680
AhlI ACTAGT 2 cut(s) 85, 260
AjnI CCWGG 1 cut(s) 628
AluBI AGCT 4 cut(s) 182, 191, 375, 525
AluI AGCT 4 cut(s) 182, 191, 375, 525
Alw26I GTCTC 2 cut(s) 272, 401
AlwI GGATC 1 cut(s) 457
AoxI GGCC 2 cut(s) 143, 626
ApeKI GCWGC 5 cut(s) 188, 191, 233, 375, 525
ApoI RAATTY 2 cut(s) 301, 423
AspS9I GGNCC 1 cut(s) 632
AsuHPI GGTGA 1 cut(s) 298
AvaII GGWCC 1 cut(s) 632
BalI TGGCCA 1 cut(s) 145
BarI GAAGNNNNNNTAC 2 cut(s) 198, 230
BbvI GCAGC 5 cut(s) 178, 200, 220, 362, 512
BccI CCATC 3 cut(s) 311, 643, 647
BceAI ACGGC 2 cut(s) 146, 553
BciT130I CCWGG 1 cut(s) 630
BcoDI GTCTC 2 cut(s) 272, 401
BcuI ACTAGT 2 cut(s) 85, 260
BfaI CTAG 3 cut(s) 86, 261, 587
BfmI CTRYAG 2 cut(s) 48, 186
BisI GCNGC 5 cut(s) 189, 192, 234, 376, 526
BlpI GCTNAGC 1 cut(s) 379
BlsI GCNGC 5 cut(s) 190, 193, 235, 377, 527
Bme1390I CCNGG 1 cut(s) 630
Bme18I GGWCC 1 cut(s) 632
BmgT120I GGNCC 1 cut(s) 632
BmrFI CCNGG 1 cut(s) 630
BmsI GCATC 3 cut(s) 96, 142, 555
Bpu1102I GCTNAGC 1 cut(s) 379
BsaWI WCCGGW 1 cut(s) 283
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse1I ACTGG 1 cut(s) 116
Bse3DI GCAATG 1 cut(s) 158
BseBI CCWGG 1 cut(s) 630
BseGI GGATG 3 cut(s) 133, 410, 635
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMI GCAATG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 116
BseXI GCAGC 5 cut(s) 178, 200, 220, 362, 512
BshFI GGCC 2 cut(s) 145, 628
BsiSI CCGG 2 cut(s) 284, 555
BslI CCNNNNNNNGG 1 cut(s) 575
BsmAI GTCTC 2 cut(s) 272, 401
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 2 cut(s) 145, 628
Bsp143I GATC 3 cut(s) 9, 250, 462
Bsp1720I GCTNAGC 1 cut(s) 379
BspACI CCGC 1 cut(s) 66
BspANI GGCC 2 cut(s) 145, 628
BspMAI CTGCAG 1 cut(s) 190
BspPI GGATC 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 158
BsrI ACTGG 1 cut(s) 116
BssMI GATC 3 cut(s) 9, 250, 462
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 1 cut(s) 541
BstDEI CTNAG 2 cut(s) 379, 491
BstF5I GGATG 3 cut(s) 133, 410, 635
BstKTI GATC 3 cut(s) 12, 253, 465
BstMAI GTCTC 2 cut(s) 272, 401
BstMBI GATC 3 cut(s) 9, 250, 462
BstMWI GCNNNNNNNGC 2 cut(s) 188, 551
BstNI CCWGG 1 cut(s) 630
BstSCI CCNGG 1 cut(s) 628
BstSFI CTRYAG 2 cut(s) 48, 186
BstV1I GCAGC 5 cut(s) 178, 200, 220, 362, 512
BstX2I RGATCY 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 153
BstYI RGATCY 1 cut(s) 462
BsuRI GGCC 2 cut(s) 145, 628
BtsCI GGATG 3 cut(s) 133, 410, 635
Cfr13I GGNCC 1 cut(s) 632
Csp6I GTAC 2 cut(s) 206, 652
CviAII CATG 2 cut(s) 7, 196
CviQI GTAC 2 cut(s) 206, 652
DdeI CTNAG 2 cut(s) 379, 491
DpnI GATC 3 cut(s) 11, 252, 464
DpnII GATC 3 cut(s) 9, 250, 462
DraI TTTAAA 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 632
EcoRII CCWGG 1 cut(s) 628
FaeI CATG 2 cut(s) 10, 199
FalI AAGNNNNNCTT 2 cut(s) 587, 619
FatI CATG 2 cut(s) 6, 195
Fnu4HI GCNGC 5 cut(s) 189, 192, 234, 376, 526
FokI GGATG 3 cut(s) 120, 417, 622
Fsp4HI GCNGC 5 cut(s) 189, 192, 234, 376, 526
FspBI CTAG 3 cut(s) 86, 261, 587
GluI GCNGC 5 cut(s) 189, 192, 234, 376, 526
HaeIII GGCC 2 cut(s) 145, 628
HapII CCGG 2 cut(s) 284, 555
Hin1II CATG 2 cut(s) 10, 199
HincII GTYRAC 1 cut(s) 323
HindII GTYRAC 1 cut(s) 323
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 5 cut(s) 44, 69, 280, 519, 610
HpaII CCGG 2 cut(s) 284, 555
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 5 cut(s) 83, 323, 329, 654, 672
Hpy188I TCNGA 1 cut(s) 502
Hpy188III TCNNGA 4 cut(s) 218, 402, 460, 516
Hpy8I GTNNAC 5 cut(s) 83, 323, 329, 654, 672
HpyAV CCTTC 2 cut(s) 224, 349
HpyCH4III ACNGT 1 cut(s) 541
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 3 cut(s) 95, 188, 528
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 551
HpyF3I CTNAG 2 cut(s) 379, 491
HpySE526I ACGT 1 cut(s) 325
Hsp92II CATG 2 cut(s) 10, 199
Kzo9I GATC 3 cut(s) 9, 250, 462
Lsp1109I GCAGC 5 cut(s) 178, 200, 220, 362, 512
LweI GCATC 3 cut(s) 96, 142, 555
MaeI CTAG 3 cut(s) 86, 261, 587
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 1 cut(s) 474
MalI GATC 3 cut(s) 11, 252, 464
MboI GATC 3 cut(s) 9, 250, 462
MboII GAAGA 4 cut(s) 260, 278, 326, 651
MflI RGATCY 1 cut(s) 462
MlsI TGGCCA 1 cut(s) 145
MluCI AATT 6 cut(s) 163, 301, 348, 391, 423, 675
MluNI TGGCCA 1 cut(s) 145
MlyI GAGTC 2 cut(s) 63, 274
MnlI CCTC 4 cut(s) 71, 282, 476, 479
Mox20I TGGCCA 1 cut(s) 145
MscI TGGCCA 1 cut(s) 145
MseI TTAA 3 cut(s) 18, 351, 606
Msp20I TGGCCA 1 cut(s) 145
MspA1I CMGCKG 2 cut(s) 191, 525
MspI CCGG 2 cut(s) 284, 555
MspR9I CCNGG 1 cut(s) 630
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 1 cut(s) 630
MwoI GCNNNNNNNGC 2 cut(s) 188, 551
NdeII GATC 3 cut(s) 9, 250, 462
NlaIII CATG 2 cut(s) 10, 199
NmuCI GTSAC 1 cut(s) 474
PctI GAATGC 1 cut(s) 97
PfeI GAWTC 3 cut(s) 44, 519, 610
PkrI GCNGC 5 cut(s) 190, 193, 235, 377, 527
PleI GAGTC 2 cut(s) 63, 274
PpsI GAGTC 2 cut(s) 63, 274
PsiI TTATAA 2 cut(s) 414, 701
Psp6I CCWGG 1 cut(s) 628
PspGI CCWGG 1 cut(s) 628
PspPI GGNCC 1 cut(s) 632
PstI CTGCAG 1 cut(s) 190
PsuI RGATCY 1 cut(s) 462
PvuII CAGCTG 2 cut(s) 191, 525
RsaI GTAC 2 cut(s) 207, 653
RsaNI GTAC 2 cut(s) 206, 652
SaqAI TTAA 3 cut(s) 18, 351, 606
SatI GCNGC 5 cut(s) 189, 192, 234, 376, 526
Sau3AI GATC 3 cut(s) 9, 250, 462
Sau96I GGNCC 1 cut(s) 632
SchI GAGTC 2 cut(s) 63, 274
ScrFI CCNGG 1 cut(s) 630
SetI ASST 7 cut(s) 82, 184, 193, 328, 360, 377, 527
SfaNI GCATC 3 cut(s) 96, 142, 555
SfcI CTRYAG 2 cut(s) 48, 186
SinI GGWCC 1 cut(s) 632
SpeI ACTAGT 2 cut(s) 85, 260
Sse9I AATT 6 cut(s) 163, 301, 348, 391, 423, 675
SsiI CCGC 1 cut(s) 66
SspMI CTAG 3 cut(s) 86, 261, 587
StyD4I CCNGG 1 cut(s) 628
TaaI ACNGT 1 cut(s) 541
TaiI ACGT 1 cut(s) 328
TaqI TCGA 1 cut(s) 121
TasI AATT 6 cut(s) 163, 301, 348, 391, 423, 675
TatI WGTACW 1 cut(s) 205
TfiI GAWTC 3 cut(s) 44, 519, 610
Tru1I TTAA 3 cut(s) 18, 351, 606
Tru9I TTAA 3 cut(s) 18, 351, 606
TseFI GTSAC 1 cut(s) 474
TseI GCWGC 5 cut(s) 188, 191, 233, 375, 525
Tsp45I GTSAC 1 cut(s) 474
TspDTI ATGAA 2 cut(s) 17, 314
TspGWI ACGGA 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 632
XapI RAATTY 2 cut(s) 301, 423
XspI CTAG 3 cut(s) 86, 261, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.