Rroxscaffold_6G00407000

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0018139
Physical Location & Seq
Forward (+)
29534234 .. 29535678
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00407000.1

Sequence Viewer

Length: 693 bp
ATGGAGGAGCAAGCAGAGGTAGTAAGCAGTTTTGGACATGAAAGTTATGATGCAGTTGTTGTGGGGTCTGGTTATGGAGGTTCTGTTGCTGCTTGTCGGCTGGCTATGGCAGGCGTAAGGGTGTGTCTACTTGAAAAAGGCCGGAAATGGGAAGCGCAGGACTTCCCAACTGACAGCTTCAAAATCTTATCGGCTGTGAGGATGGAGAACCAGAACCTAGGTGTTAGCTATGGACCAAAAGATGCTTTATTTCAGGTGTACGAGCAAAATGATTCTCTAGCAGCAGTGGCTTGTGGGCTTGGTGGAGGTTCACTAGTGAATGCTGGAGTTATGATGCCAGTACCTGATCGTGCTAGACGGCATCCAAAATGGCCGAAGGAATGGGAAAGGAATTGGCGTAGTTGTGAAGCTTCTGCTGCAGACATGCTGAGAGTACAAAGCATTCCCGTCAAGTTTCCTTCCACGAAAATTTTGGAAGAAGCTGCTGATGGAGTAACTTTTGACACTTCAATAAAGCTTAGTGTGAATTTTGACATTGAAGAACCAACAACAAATGGAACGAAGCGTCCACAGATGGGTAGCTGCTTAGCCTGTGGAAACTGTCTTTCTGGATGTCCATATAACGCCAAAAGTAAGAAACAGTCAAAGTTTGTATGTTCAAGAAATTCTGAAGATCACAAAGATGTTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

230

Amino Acids

25.04

Weight (kDa)

6.33

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 20 - 48 6.4e-06 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018139)

Species Orthologous Gene IDs
pyrus_communis pycom10g15180 pycom10g15190
rosa_chinensis RchiOBHm_Chr3g0474671
rosa_multiflora Rmu_sc0001353.1_g000047 Rmu_sc0002324.1_g000028
rosa_roxburghii Rroxscaffold_6G00407000
rosa_rugosa Rorug03G0142000
rosa_samantha Rh3AG192800 Rh3BG222700 Rh3CG217400 Rh3DG218300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 127
AcoI YGGCCR 1 cut(s) 371
AcsI RAATTY 3 cut(s) 468, 526, 664
AcuI CTGAAG 1 cut(s) 690
AfaI GTAC 3 cut(s) 260, 342, 435
AfiI CCNNNNNNNGG 2 cut(s) 148, 575
AgsI TTSAA 5 cut(s) 134, 181, 510, 539, 660
AhlI ACTAGT 1 cut(s) 313
AloI GAACNNNNNNTCC 2 cut(s) 550, 582
AluBI AGCT 6 cut(s) 177, 228, 410, 482, 517, 582
AluI AGCT 6 cut(s) 177, 228, 410, 482, 517, 582
AlwNI CAGNNNCTG 1 cut(s) 344
AoxI GGCC 2 cut(s) 139, 371
ApeKI GCWGC 5 cut(s) 89, 281, 416, 482, 582
ApoI RAATTY 3 cut(s) 468, 526, 664
AspA2I CCTAGG 1 cut(s) 217
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 233
AvaII GGWCC 1 cut(s) 233
AvrII CCTAGG 1 cut(s) 217
BbvI GCAGC 5 cut(s) 76, 293, 403, 469, 569
BccI CCATC 3 cut(s) 196, 482, 568
BceAI ACGGC 1 cut(s) 374
BcuI ACTAGT 1 cut(s) 313
BfaI CTAG 5 cut(s) 218, 278, 314, 354, 691
BfmI CTRYAG 1 cut(s) 417
BisI GCNGC 5 cut(s) 90, 282, 417, 483, 583
BlnI CCTAGG 1 cut(s) 217
BlpI GCTNAGC 1 cut(s) 586
BlsI GCNGC 5 cut(s) 91, 283, 418, 484, 584
Bme18I GGWCC 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 233
BmsI GCATC 4 cut(s) 40, 232, 324, 370
BpmI CTGGAG 1 cut(s) 345
Bpu1102I GCTNAGC 1 cut(s) 586
BsaJI CCNNGG 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 575
Bse1I ACTGG 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 3 cut(s) 207, 361, 617
BseLI CCNNNNNNNGG 2 cut(s) 148, 575
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 1 cut(s) 338
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 5 cut(s) 76, 293, 403, 469, 569
BshFI GGCC 2 cut(s) 141, 373
BsiSI CCGG 1 cut(s) 142
BslI CCNNNNNNNGG 2 cut(s) 148, 575
BsmI GAATGC 2 cut(s) 325, 441
BsnI GGCC 2 cut(s) 141, 373
Bsp143I GATC 2 cut(s) 346, 673
Bsp1720I GCTNAGC 1 cut(s) 586
BspANI GGCC 2 cut(s) 141, 373
BspCNI CTCAG 1 cut(s) 420
BspMAI CTGCAG 1 cut(s) 421
BsrI ACTGG 1 cut(s) 338
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 2 cut(s) 346, 673
BssT1I CCWWGG 1 cut(s) 217
Bst4CI ACNGT 2 cut(s) 602, 642
BstC8I GCNNGC 3 cut(s) 12, 102, 112
BstDEI CTNAG 3 cut(s) 428, 518, 586
BstF5I GGATG 3 cut(s) 207, 361, 617
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 2 cut(s) 349, 676
BstMBI GATC 2 cut(s) 346, 673
BstMWI GCNNNNNNNGC 2 cut(s) 287, 416
BstNSI RCATGY 1 cut(s) 427
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 5 cut(s) 76, 293, 403, 469, 569
BsuRI GGCC 2 cut(s) 141, 373
BtsCI GGATG 3 cut(s) 207, 361, 617
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 291
Cac8I GCNNGC 3 cut(s) 12, 102, 112
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 233
CseI GACGC 1 cut(s) 554
Csp6I GTAC 3 cut(s) 259, 341, 434
CviAII CATG 2 cut(s) 38, 424
CviQI GTAC 3 cut(s) 259, 341, 434
DdeI CTNAG 3 cut(s) 428, 518, 586
DpnI GATC 2 cut(s) 348, 675
DpnII GATC 2 cut(s) 346, 673
EaeI YGGCCR 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 690
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 2 cut(s) 41, 427
FatI CATG 2 cut(s) 37, 423
FblI GTMKAC 1 cut(s) 127
Fnu4HI GCNGC 5 cut(s) 90, 282, 417, 483, 583
FokI GGATG 3 cut(s) 214, 348, 624
Fsp4HI GCNGC 5 cut(s) 90, 282, 417, 483, 583
FspBI CTAG 5 cut(s) 218, 278, 314, 354, 691
GlaI GCGC 1 cut(s) 156
GluI GCNGC 5 cut(s) 90, 282, 417, 483, 583
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 2 cut(s) 141, 373
HapII CCGG 1 cut(s) 142
HgaI GACGC 1 cut(s) 554
HhaI GCGC 1 cut(s) 157
Hin1II CATG 2 cut(s) 41, 427
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 2 cut(s) 408, 515
HinfI GANTC 1 cut(s) 272
HpaI GTTAAC 1 cut(s) 688
HpaII CCGG 1 cut(s) 142
Hpy166II GTNNAC 5 cut(s) 128, 259, 311, 569, 688
Hpy188I TCNGA 1 cut(s) 670
Hpy188III TCNNGA 2 cut(s) 609, 660
Hpy8I GTNNAC 5 cut(s) 128, 259, 311, 569, 688
HpyAV CCTTC 2 cut(s) 370, 468
HpyCH4III ACNGT 2 cut(s) 602, 642
HpyCH4V TGCA 2 cut(s) 53, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 416
HpyF3I CTNAG 3 cut(s) 428, 518, 586
Hsp92II CATG 2 cut(s) 41, 427
HspAI GCGC 1 cut(s) 155
KspAI GTTAAC 1 cut(s) 688
Kzo9I GATC 2 cut(s) 346, 673
LmnI GCTCC 1 cut(s) 7
Lsp1109I GCAGC 5 cut(s) 76, 293, 403, 469, 569
LweI GCATC 4 cut(s) 40, 232, 324, 370
MaeI CTAG 5 cut(s) 218, 278, 314, 354, 691
MaeIII GTNAC 1 cut(s) 493
MalI GATC 2 cut(s) 348, 675
MboI GATC 2 cut(s) 346, 673
MboII GAAGA 3 cut(s) 488, 551, 683
MluCI AATT 4 cut(s) 391, 468, 526, 664
MnlI CCTC 4 cut(s) 10, 71, 192, 299
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 1 cut(s) 681
MspI CCGG 1 cut(s) 142
Mva1269I GAATGC 2 cut(s) 325, 441
MwoI GCNNNNNNNGC 2 cut(s) 287, 416
NdeII GATC 2 cut(s) 346, 673
NlaIII CATG 2 cut(s) 41, 427
NspI RCATGY 1 cut(s) 427
PctI GAATGC 2 cut(s) 325, 441
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 5 cut(s) 91, 283, 418, 484, 584
PspPI GGNCC 1 cut(s) 233
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 344
RsaI GTAC 3 cut(s) 260, 342, 435
RsaNI GTAC 3 cut(s) 259, 341, 434
RseI CAYNNNNRTG 1 cut(s) 681
SaqAI TTAA 1 cut(s) 687
SatI GCNGC 5 cut(s) 90, 282, 417, 483, 583
Sau3AI GATC 2 cut(s) 346, 673
Sau96I GGNCC 1 cut(s) 233
SfaNI GCATC 4 cut(s) 40, 232, 324, 370
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 681
SpeI ACTAGT 1 cut(s) 313
Sse9I AATT 4 cut(s) 391, 468, 526, 664
SspMI CTAG 5 cut(s) 218, 278, 314, 354, 691
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 2 cut(s) 602, 642
TasI AATT 4 cut(s) 391, 468, 526, 664
TatI WGTACW 1 cut(s) 433
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 1 cut(s) 291
TseI GCWGC 5 cut(s) 89, 281, 416, 482, 582
TspDTI ATGAA 1 cut(s) 54
TspRI CASTG 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 3 cut(s) 468, 526, 664
XceI RCATGY 1 cut(s) 427
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XmaJI CCTAGG 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 127
XspI CTAG 5 cut(s) 218, 278, 314, 354, 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.