pycom10g16760

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0016219
Physical Location & Seq
Forward (+)
19894378 .. 19896101
1724 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGACCCCAAAACAACCTCACAAAGCTTACCCTATCTTTTTGTGGGATATAAATACCAAAAATACATGCAACTTTCACTACTACTCATCCATGGCTGCTTTCAACATGTTTAAGCTCTCATTTTTCTTGAGCCTTCTCTCCCATCTGAACCAGGGGTTGTGGCCACAAAATCAAAACAGTTTGGTAATATTTCCACCATCATGTAACAGCATAGAGTGCCCTAGCTTCGACACAGTCGAGGTTGGAAATGGCTACGAAGTCCGTCGATACAATTCGACAGAGTGGATATCGACTTCTCCTATTCAAGATATCTCTCTTGTTGAAGCCACCAGAACTGGCTTCTTACAGTTGTTTGACTACATTCAAGGGAAGAACAAGTATGAAGAGAAAATAGAGATGACTGCCCCAGTCCTCAGCGAAGTCTCACCAAGTGATGGACCCTTCTGTGAGTCCTCATTTGTTGTGAGCTTCTATGTGCCAAAGAAGAACCAAGAAAACCCACCACCAGCAGAAGGTCTTCATCTTCAAACATGGAAACCTAAATATGTGGCAGTGAGACAATTCACTGGATTCGTATCTGACTACAACATCGGAGAAGAAGCTGCTGCCTTGCAGGCCAGTCTTGCAGGCACTATTTGGTCTGCTGCCATTGACAAAAGCCATGCAGCCGATCACACTTCAGCTTATACTGTTGCGCAGTACAATTCACCGTTCGAATTTGATCATAGGGTGAATGAGATATGGATGATATTTGATTTAGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

256

Amino Acids

29.02

Weight (kDa)

5.06

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 71 - 249 2e-53 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17100
fragaria_vesca FvH4_3g16830
malus_domestica MD10G1193800.v1.1
prunus_persica Prupe.4G150500_v2.0.a1
pyrus_communis pycom10g16760
rosa_chinensis RchiOBHm_Chr5g0028041
rosa_laevigata RLG00000033060
rosa_multiflora Rmu_sc0009432.1_g000007
rosa_roxburghii Rroxscaffold_1G00051620
rosa_rugosa Rorug05G0103700
rosa_samantha Rh5AG197300 Rh5BG195500 Rh5CG215700 Rh5DG197100
rosa_wichuraiana Rw5G017990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 696
AccB7I CCANNNNNTGG 1 cut(s) 434
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 1 cut(s) 716
AcuI CTGAAG 1 cut(s) 663
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 1 cut(s) 701
AfiI CCNNNNNNNGG 2 cut(s) 434, 512
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 5 cut(s) 103, 305, 323, 365, 527
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 6 cut(s) 26, 115, 225, 468, 602, 683
AluI AGCT 6 cut(s) 26, 115, 225, 468, 602, 683
Alw26I GTCTC 2 cut(s) 427, 550
AoxI GGCC 2 cut(s) 161, 615
ApeKI GCWGC 5 cut(s) 95, 602, 605, 644, 665
ApoI RAATTY 1 cut(s) 716
Asp700I GAANNNNTTC 1 cut(s) 516
AspLEI GCGC 1 cut(s) 697
AspS9I GGNCC 1 cut(s) 437
AsuHPI GGTGA 3 cut(s) 417, 699, 742
AsuII TTCGAA 1 cut(s) 714
AvaII GGWCC 1 cut(s) 437
BaeGI GKGCMC 1 cut(s) 221
BalI TGGCCA 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 509
BbvCI CCTCAGC 1 cut(s) 413
BbvI GCAGC 5 cut(s) 82, 589, 592, 631, 677
BccI CCATC 3 cut(s) 150, 205, 428
BciT130I CCWGG 1 cut(s) 152
BclI TGATCA 1 cut(s) 721
BcoDI GTCTC 2 cut(s) 427, 550
BfaI CTAG 1 cut(s) 222
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 5 cut(s) 96, 603, 606, 645, 666
BlsI GCNGC 5 cut(s) 97, 604, 607, 646, 667
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 152
BmrI ACTGGG 1 cut(s) 401
BmuI ACTGGG 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 509
Bpu10I CCTNAGC 1 cut(s) 413
Bpu14I TTCGAA 1 cut(s) 714
BpuEI CTTGAG 1 cut(s) 148
BsaBI GATNNNNATC 1 cut(s) 574
BsaJI CCNNGG 2 cut(s) 90, 151
Bsc4I CCNNNNNNNGG 2 cut(s) 434, 512
Bse1I ACTGG 4 cut(s) 340, 407, 571, 618
Bse8I GATNNNNATC 1 cut(s) 574
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 2 cut(s) 90, 151
BseGI GGATG 2 cut(s) 86, 750
BseJI GATNNNNATC 1 cut(s) 574
BseLI CCNNNNNNNGG 2 cut(s) 434, 512
BseMII CTCAG 1 cut(s) 427
BseNI ACTGG 4 cut(s) 340, 407, 571, 618
BseSI GKGCMC 1 cut(s) 221
BseXI GCAGC 5 cut(s) 82, 589, 592, 631, 677
BshFI GGCC 2 cut(s) 163, 617
BslI CCNNNNNNNGG 2 cut(s) 434, 512
BsmAI GTCTC 2 cut(s) 427, 550
BsnI GGCC 2 cut(s) 163, 617
Bsp119I TTCGAA 1 cut(s) 714
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 2 cut(s) 670, 721
Bsp19I CCATGG 1 cut(s) 90
BspANI GGCC 2 cut(s) 163, 617
BspCNI CTCAG 1 cut(s) 426
BspLI GGNNCC 1 cut(s) 439
BspT104I TTCGAA 1 cut(s) 714
BsrI ACTGG 4 cut(s) 340, 407, 571, 618
BssECI CCNNGG 2 cut(s) 90, 151
BssMI GATC 2 cut(s) 670, 721
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 5 cut(s) 179, 235, 348, 691, 711
Bst6I CTCTTC 2 cut(s) 378, 756
BstAPI GCANNNNNTGC 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 714
BstC8I GCNNGC 2 cut(s) 615, 628
BstDEI CTNAG 1 cut(s) 413
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 2 cut(s) 86, 750
BstHHI GCGC 1 cut(s) 697
BstKTI GATC 2 cut(s) 673, 724
BstMAI GTCTC 2 cut(s) 427, 550
BstMBI GATC 2 cut(s) 670, 721
BstMWI GCNNNNNNNGC 3 cut(s) 216, 614, 623
BstNI CCWGG 1 cut(s) 152
BstNSI RCATGY 2 cut(s) 69, 109
BstSCI CCNGG 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 221
BstV1I GCAGC 5 cut(s) 82, 589, 592, 631, 677
BstV2I GAAGAC 1 cut(s) 509
BsuRI GGCC 2 cut(s) 163, 617
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 2 cut(s) 86, 750
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 2 cut(s) 558, 564
Cac8I GCNNGC 2 cut(s) 615, 628
CfoI GCGC 1 cut(s) 697
Cfr13I GGNCC 1 cut(s) 437
Csp6I GTAC 1 cut(s) 700
CviAII CATG 6 cut(s) 66, 91, 106, 201, 531, 662
CviQI GTAC 1 cut(s) 700
DdeI CTNAG 1 cut(s) 413
DpnI GATC 2 cut(s) 672, 723
DpnII GATC 2 cut(s) 670, 721
DraIII CACNNNGTG 1 cut(s) 431
EaeI YGGCCR 1 cut(s) 161
Eam1104I CTCTTC 2 cut(s) 378, 756
EarI CTCTTC 2 cut(s) 378, 756
Eco130I CCWWGG 1 cut(s) 90
Eco32I GATATC 2 cut(s) 288, 310
Eco47I GGWCC 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 663
EcoRII CCWGG 1 cut(s) 150
EcoRV GATATC 2 cut(s) 288, 310
EcoT14I CCWWGG 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 6 cut(s) 69, 94, 109, 204, 534, 665
FatI CATG 6 cut(s) 65, 90, 105, 200, 530, 661
FbaI TGATCA 1 cut(s) 721
Fnu4HI GCNGC 5 cut(s) 96, 603, 606, 645, 666
FokI GGATG 2 cut(s) 73, 757
Fsp4HI GCNGC 5 cut(s) 96, 603, 606, 645, 666
FspBI CTAG 1 cut(s) 222
FspI TGCGCA 1 cut(s) 696
GlaI GCGC 1 cut(s) 696
GluI GCNGC 5 cut(s) 96, 603, 606, 645, 666
HaeIII GGCC 2 cut(s) 163, 617
HhaI GCGC 1 cut(s) 697
Hin1II CATG 6 cut(s) 69, 94, 109, 204, 534, 665
Hin6I GCGC 1 cut(s) 695
HinP1I GCGC 1 cut(s) 695
HindIII AAGCTT 1 cut(s) 24
HinfI GANTC 2 cut(s) 449, 570
HphI GGTGA 3 cut(s) 417, 699, 742
Hpy188I TCNGA 3 cut(s) 147, 580, 593
Hpy188III TCNNGA 2 cut(s) 127, 305
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 3 cut(s) 143, 451, 506
HpyCH4III ACNGT 5 cut(s) 179, 235, 348, 691, 711
HpyCH4V TGCA 4 cut(s) 69, 613, 626, 665
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 614, 623
HpyF3I CTNAG 1 cut(s) 413
Hsp92II CATG 6 cut(s) 69, 94, 109, 204, 534, 665
HspAI GCGC 1 cut(s) 695
Ksp22I TGATCA 1 cut(s) 721
Kzo9I GATC 2 cut(s) 670, 721
Lsp1109I GCAGC 5 cut(s) 82, 589, 592, 631, 677
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 203
MalI GATC 2 cut(s) 672, 723
MboI GATC 2 cut(s) 670, 721
MboII GAAGA 6 cut(s) 382, 395, 496, 509, 515, 608
MhlI GDGCHC 1 cut(s) 221
MlsI TGGCCA 1 cut(s) 163
MluCI AATT 4 cut(s) 271, 560, 703, 716
MluNI TGGCCA 1 cut(s) 163
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 4 cut(s) 27, 232, 422, 463
Mox20I TGGCCA 1 cut(s) 163
MroXI GAANNNNTTC 1 cut(s) 516
MscI TGGCCA 1 cut(s) 163
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 199
Msp20I TGGCCA 1 cut(s) 163
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
MwoI GCNNNNNNNGC 3 cut(s) 216, 614, 623
NcoI CCATGG 1 cut(s) 90
NdeII GATC 2 cut(s) 670, 721
NlaIII CATG 6 cut(s) 69, 94, 109, 204, 534, 665
NlaIV GGNNCC 1 cut(s) 439
NsbI TGCGCA 1 cut(s) 696
NspI RCATGY 2 cut(s) 69, 109
NspV TTCGAA 1 cut(s) 714
PciI ACATGT 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 1 cut(s) 570
PflFI GACNNNGTC 1 cut(s) 233
PflMI CCANNNNNTGG 1 cut(s) 434
PkrI GCNGC 5 cut(s) 97, 604, 607, 646, 667
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PscI ACATGT 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 437
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 1 cut(s) 701
RsaNI GTAC 1 cut(s) 700
RseI CAYNNNNRTG 1 cut(s) 199
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 5 cut(s) 96, 603, 606, 645, 666
Sau3AI GATC 2 cut(s) 670, 721
Sau96I GGNCC 1 cut(s) 437
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 1 cut(s) 221
SfuI TTCGAA 1 cut(s) 714
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 199
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
Sse9I AATT 4 cut(s) 271, 560, 703, 716
SspI AATATT 1 cut(s) 189
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 150
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 5 cut(s) 179, 235, 348, 691, 711
TaqI TCGA 6 cut(s) 228, 237, 265, 275, 290, 714
TasI AATT 4 cut(s) 271, 560, 703, 716
TatI WGTACW 1 cut(s) 699
TfiI GAWTC 1 cut(s) 570
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 2 cut(s) 558, 571
TseI GCWGC 5 cut(s) 95, 602, 605, 644, 665
TspDTI ATGAA 2 cut(s) 396, 509
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 2 cut(s) 558, 571
Tth111I GACNNNGTC 1 cut(s) 233
Van91I CCANNNNNTGG 1 cut(s) 434
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 1 cut(s) 716
XceI RCATGY 2 cut(s) 69, 109
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.