pycom10g17360

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013520
Physical Location & Seq
Reverse (-)
20572100 .. 20572990
891 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 891 bp
ATGGCCGTCATAGAAAAATACCCTGCAATATTCTATGTCGGTGGCGGCAACAGAACGCCCCCTTTTGTTACACTCGCTGAGAAGGCTGAGAAAGTTGCTGGTGAAGAAGCTTCAGCCAAGGATTTGATGGAACCCATGTTGGTTAAAAACTTGAGGAAGTTGTTGATGTTGTCGGTTGATTGCCGGGTTCCACTCGAAAACATTGAATTCATCGAATCCGAATTGGGTTTGCCGTATGATTTCAAGAAATCATTGATTCCGAAGTACCCTGAATTCTTTTCGGTTAAGGATGTCAATGAAAAAGATTTTCTGCATTTGGAGAATTGGGATTCTTCACTAGCAATCACGGCTCGTGAGGAAAATTTAGCCAATGAAGGAATTTCTGCCGCTTATGGATTGGGGAAGAAGGTTAGGATATCCAAAGATGGTAATTTTCTAGGTCCACATGCGTTTCGAATGAATTTTCCTGCTGGTTTTAGGCCAAACACTAGTTATCTTGAGCAACTTGAAAGGTGGCAGAAAATGGAATTCCCTTCTCCATATTTGAATGCCAGGAGATTTGACATTGCTGATCCCAAATCGAGGAAGCGGGTGGTGGCGGTGCTTCATGAGCTACTCAGCTTGACAATGCAGAAGAGGATGACATCTGCTCAACTGGAAGCATTCCATTCAGAGTATTTCTTACCCTCTAAATTGCTCCTTTGTCTGATAAAGCATCCTGGTATCTTTTACATAACTAATAAAGGTGCAAGGAGCACTGTTTTTCTTAAAGAAGCTTATGATGGATTGAATTTGATAAACAAATGTCCCCTGTTGTCATTTTATGATAAATTTGTAGCGCTTAGCGGTAGGAGAGAGACCAATCCATGTAACGTGATGCATTCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

297

Amino Acids

33.71

Weight (kDa)

8.86

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 2 - 285 3.7e-92 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013520)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79120 AT1G79120
fragaria_vesca FvH4_5g24801
malus_domestica MD05G1220600.v1.1 MD10G1203000.v1.1
prunus_persica Prupe.4G139300_v2.0.a1
pyrus_communis pycom05g20410 pycom10g17360
rosa_chinensis RchiOBHm_Chr7g0215141
rosa_laevigata RLG00000002690
rosa_multiflora Rmu_sc0005476.1_g000003
rosa_roxburghii Rroxscaffold_3G00244590
rosa_rugosa Rorug07G0150200
rosa_samantha Rh7BG278800 Rh7CG307000 Rh7DG290600 Rh7DG397500
rosa_wichuraiana Rw7G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 45, 387, 589, 599, 846
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 8 cut(s) 206, 272, 361, 378, 460, 527, 790, 830
AcuI CTGAAG 1 cut(s) 96
AfaI GTAC 1 cut(s) 266
AfeI AGCGCT 1 cut(s) 840
AfiI CCNNNNNNNGG 1 cut(s) 582
AgsI TTSAA 5 cut(s) 206, 244, 509, 547, 790
AhlI ACTAGT 1 cut(s) 488
AjnI CCWGG 2 cut(s) 551, 718
AluBI AGCT 4 cut(s) 110, 613, 621, 776
AluI AGCT 4 cut(s) 110, 613, 621, 776
Alw21I GWGCWC 1 cut(s) 758
Alw26I GTCTC 1 cut(s) 851
AlwI GGATC 1 cut(s) 566
Aor51HI AGCGCT 1 cut(s) 840
AoxI GGCC 2 cut(s) 3, 479
ApoI RAATTY 8 cut(s) 206, 272, 361, 378, 460, 527, 790, 830
AspLEI GCGC 1 cut(s) 841
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 113
AsuII TTCGAA 1 cut(s) 454
AvaII GGWCC 1 cut(s) 440
BauI CACGAG 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 758
BccI CCATC 3 cut(s) 121, 419, 776
BceAI ACGGC 2 cut(s) 217, 363
BciT130I CCWGG 2 cut(s) 553, 720
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 851
BcuI ACTAGT 1 cut(s) 488
BfaI CTAG 3 cut(s) 338, 437, 489
BfoI RGCGCY 1 cut(s) 842
BisI GCNGC 2 cut(s) 46, 387
BlpI GCTNAGC 1 cut(s) 842
BlsI GCNGC 2 cut(s) 47, 388
Bme1390I CCNGG 3 cut(s) 185, 553, 720
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 132, 189
BmrFI CCNGG 3 cut(s) 185, 553, 720
BmsI GCATC 2 cut(s) 724, 867
Bpu1102I GCTNAGC 1 cut(s) 842
Bpu14I TTCGAA 1 cut(s) 454
BpuEI CTTGAG 2 cut(s) 172, 518
BpuMI CCSGG 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 1 cut(s) 117
Bsc4I CCNNNNNNNGG 1 cut(s) 582
Bse1I ACTGG 1 cut(s) 660
Bse3DI GCAATG 1 cut(s) 564
BseBI CCWGG 2 cut(s) 553, 720
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 3 cut(s) 295, 645, 715
BseLI CCNNNNNNNGG 1 cut(s) 582
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 3 cut(s) 69, 78, 631
BseNI ACTGG 1 cut(s) 660
BshFI GGCC 2 cut(s) 5, 481
BsiHKAI GWGCWC 1 cut(s) 758
BsiSI CCGG 1 cut(s) 184
BslFI GGGAC 1 cut(s) 792
BslI CCNNNNNNNGG 1 cut(s) 582
BsmAI GTCTC 1 cut(s) 851
BsmFI GGGAC 1 cut(s) 792
BsmI GAATGC 3 cut(s) 553, 662, 880
BsnI GGCC 2 cut(s) 5, 481
Bso31I GGTCTC 1 cut(s) 851
Bsp119I TTCGAA 1 cut(s) 454
Bsp1286I GDGCHC 1 cut(s) 758
Bsp143I GATC 1 cut(s) 571
Bsp1720I GCTNAGC 1 cut(s) 842
BspACI CCGC 5 cut(s) 45, 387, 589, 599, 846
BspANI GGCC 2 cut(s) 5, 481
BspCNI CTCAG 3 cut(s) 70, 79, 630
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 132, 189
BspPI GGATC 1 cut(s) 566
BspT104I TTCGAA 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 851
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 1 cut(s) 660
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 1 cut(s) 571
BssSI CACGAG 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 117
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 2 cut(s) 553, 720
Bst4CI ACNGT 1 cut(s) 760
Bst6I CTCTTC 1 cut(s) 629
BstBI TTCGAA 1 cut(s) 454
BstDEI CTNAG 4 cut(s) 78, 87, 617, 842
BstF5I GGATG 3 cut(s) 295, 645, 715
BstH2I RGCGCY 1 cut(s) 842
BstHHI GCGC 1 cut(s) 841
BstKTI GATC 1 cut(s) 574
BstMAI GTCTC 1 cut(s) 851
BstMBI GATC 1 cut(s) 571
BstMWI GCNNNNNNNGC 3 cut(s) 83, 347, 610
BstNI CCWGG 2 cut(s) 553, 720
BstNSI RCATGY 1 cut(s) 449
BstSCI CCNGG 3 cut(s) 183, 551, 718
BsuRI GGCC 2 cut(s) 5, 481
BtsCI GGATG 3 cut(s) 295, 645, 715
BtsIMutI CAGTG 1 cut(s) 756
CciI TCATGA 1 cut(s) 607
CfoI GCGC 1 cut(s) 841
Cfr13I GGNCC 1 cut(s) 440
Csp6I GTAC 1 cut(s) 265
CviAII CATG 4 cut(s) 136, 446, 608, 867
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 4 cut(s) 78, 87, 617, 842
DpnI GATC 1 cut(s) 573
DpnII GATC 1 cut(s) 571
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 851
Eco32I GATATC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 440
Eco47III AGCGCT 1 cut(s) 840
Eco57I CTGAAG 1 cut(s) 96
EcoRI GAATTC 3 cut(s) 206, 272, 527
EcoRII CCWGG 2 cut(s) 551, 718
EcoRV GATATC 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 882
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 4 cut(s) 139, 449, 611, 870
FaqI GGGAC 1 cut(s) 792
FatI CATG 4 cut(s) 135, 445, 607, 866
FauI CCCGC 1 cut(s) 582
Fnu4HI GCNGC 2 cut(s) 46, 387
FokI GGATG 3 cut(s) 302, 652, 702
Fsp4HI GCNGC 2 cut(s) 46, 387
FspBI CTAG 3 cut(s) 338, 437, 489
GlaI GCGC 1 cut(s) 840
GluI GCNGC 2 cut(s) 46, 387
HaeII RGCGCY 1 cut(s) 842
HaeIII GGCC 2 cut(s) 5, 481
HapII CCGG 1 cut(s) 184
HhaI GCGC 1 cut(s) 841
Hin1II CATG 4 cut(s) 139, 449, 611, 870
Hin6I GCGC 1 cut(s) 839
HinP1I GCGC 1 cut(s) 839
HindIII AAGCTT 2 cut(s) 108, 774
HinfI GANTC 3 cut(s) 215, 256, 329
HpaII CCGG 1 cut(s) 184
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 4 cut(s) 220, 261, 673, 708
Hpy188III TCNNGA 4 cut(s) 244, 353, 497, 608
Hpy8I GTNNAC 1 cut(s) 443
HpyAV CCTTC 4 cut(s) 76, 368, 400, 543
HpyCH4III ACNGT 1 cut(s) 760
HpyCH4IV ACGT 1 cut(s) 873
HpyCH4V TGCA 5 cut(s) 26, 313, 631, 749, 880
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 347, 610
HpyF3I CTNAG 4 cut(s) 78, 87, 617, 842
HpySE526I ACGT 1 cut(s) 873
Hsp92II CATG 4 cut(s) 139, 449, 611, 870
HspAI GCGC 1 cut(s) 839
Kzo9I GATC 1 cut(s) 571
LmnI GCTCC 2 cut(s) 702, 753
LweI GCATC 2 cut(s) 724, 867
MaeI CTAG 3 cut(s) 338, 437, 489
MaeII ACGT 1 cut(s) 873
MaeIII GTNAC 2 cut(s) 67, 869
MalI GATC 1 cut(s) 573
MboI GATC 1 cut(s) 571
MboII GAAGA 5 cut(s) 116, 324, 415, 646, 876
MhlI GDGCHC 1 cut(s) 758
MnlI CCTC 5 cut(s) 147, 349, 576, 630, 697
Mph1103I ATGCAT 1 cut(s) 882
MseI TTAA 3 cut(s) 144, 285, 768
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 3 cut(s) 185, 553, 720
Mva1269I GAATGC 3 cut(s) 553, 662, 880
MvaI CCWGG 2 cut(s) 553, 720
MwoI GCNNNNNNNGC 3 cut(s) 83, 347, 610
NciI CCSGG 1 cut(s) 185
NdeII GATC 1 cut(s) 571
NlaIII CATG 4 cut(s) 139, 449, 611, 870
NlaIV GGNNCC 2 cut(s) 132, 189
NsiI ATGCAT 1 cut(s) 882
NspI RCATGY 1 cut(s) 449
NspV TTCGAA 1 cut(s) 454
PagI TCATGA 1 cut(s) 607
PctI GAATGC 3 cut(s) 553, 662, 880
PfeI GAWTC 3 cut(s) 215, 256, 329
PkrI GCNGC 2 cut(s) 47, 388
Psp6I CCWGG 2 cut(s) 551, 718
PspGI CCWGG 2 cut(s) 551, 718
PspN4I GGNNCC 2 cut(s) 132, 189
PspPI GGNCC 1 cut(s) 440
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
SaqAI TTAA 3 cut(s) 144, 285, 768
SatI GCNGC 2 cut(s) 46, 387
Sau3AI GATC 1 cut(s) 571
Sau96I GGNCC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 185, 553, 720
SduI GDGCHC 1 cut(s) 758
SetI ASST 9 cut(s) 112, 411, 442, 515, 615, 623, 748, 778, 876
SfaNI GCATC 2 cut(s) 724, 867
SfuI TTCGAA 1 cut(s) 454
SinI GGWCC 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 151, 497
SmoI CTYRAG 2 cut(s) 151, 497
SpeI ACTAGT 1 cut(s) 488
SsiI CCGC 5 cut(s) 45, 387, 589, 599, 846
SspI AATATT 1 cut(s) 30
SspMI CTAG 3 cut(s) 338, 437, 489
StyD4I CCNGG 3 cut(s) 183, 551, 718
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 1 cut(s) 760
TaiI ACGT 1 cut(s) 876
TaqI TCGA 4 cut(s) 195, 213, 454, 581
TauI GCSGC 2 cut(s) 48, 389
TfiI GAWTC 3 cut(s) 215, 256, 329
Tru1I TTAA 3 cut(s) 144, 285, 768
Tru9I TTAA 3 cut(s) 144, 285, 768
TscAI CASTG 1 cut(s) 763
TspDTI ATGAA 6 cut(s) 199, 312, 387, 473, 596, 876
TspRI CASTG 1 cut(s) 763
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 8 cut(s) 206, 272, 361, 378, 460, 527, 790, 830
XceI RCATGY 1 cut(s) 449
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XspI CTAG 3 cut(s) 338, 437, 489
Zsp2I ATGCAT 1 cut(s) 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.