Rorug07G0150200

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0013520
Physical Location & Seq
Forward (+)
11930218 .. 11931279
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0150200.1

Sequence Viewer

Length: 1062 bp
ATGAGCGTGGGGTTGGCTGGAGGTCTGGGTTTATTATGGAATGATGATATGAAGGTCCAAATACGGCAGCCTTCTACGGTTCATTTTATTGATGCAGTAGTAGAGGAAGGTGATGGTTTTTTGCAATGGCGCTTCACTGGGTTTTACGGCAATCCAAGAACTGCCGACAGACACGAATCTTGGGATATTCTTAGGGCTTTGGGTGATGACGACAGCCTACCGTGGGTTGTTCTTGGAGATTTCAACGAAATTTTGTTGGCATCGGAGAAATTGGATGGTAGACCGAGATCGGAGAACCAAATGCGTGGTTTCCGGGAAGCGCTGGGATATGCGGAATTGGTGGACCTGGGGTTCCATGGGTGTCCGTATACATGGAGTGACTCTGAGATGAAGGTTAGGTTGGACAGCGTGGTTGCTACGGCGGCATGGTCAGACAGCTTCGTCCACTCTCGGGTCATCCATCTACCTCCTAGCAAGTCGGACCATATTCCAATCTTGTTATGTGTTAGTTCAGTTCCTATTATCCAGAAGCAGAAATACCATAGGTTTCGATTTGAATCTCTGTGGTTGAAGTATGAGGGATGTCATGAGACCGTCCAACAGCAATGGCAGCGTCCGGTGTTGGGGCAGCCTATGTTTATGGTCTCGAAAAAGCTTGAAAGGACAAGTATTGCACTAGATGCCTGTCAACGAAGTGTGTTTGGTGATAGGCAGAAAGCAATAATGGAGATTCGTTCAAGGCTGGAGGGGCTTCTTAGTCTGTCATCGTCTCCGGCTTTGTTGGTAGAAAGGGGTAGTCTAATGGATCTCTTGGAGAGATTACTTTCGGAAGAGGAATTATATTGGAGTCAACGAGCTAAAGTCTCATGGCTTAAGGAGGGAGATCGTAACACGGGCTTCTTTCATCGGAAATCCTCTAATAGGAAAAGGAAAAATTTCATAAGGGGTCTTTACGATGAGGCAGGGGTTTGGCAGGAAGATGATGATGGGGTAGAAATTGTAATACCCAGCATTGAGACGTTAATAAAAGAATCTGTGAGCCCAGATATCGAGCTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

353

Amino Acids

40.43

Weight (kDa)

5.68

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 7 - 162 1.7e-07 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013520)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79120 AT1G79120
fragaria_vesca FvH4_5g24801
malus_domestica MD05G1220600.v1.1 MD10G1203000.v1.1
prunus_persica Prupe.4G139300_v2.0.a1
pyrus_communis pycom05g20410 pycom10g17360
rosa_chinensis RchiOBHm_Chr7g0215141
rosa_laevigata RLG00000002690
rosa_multiflora Rmu_sc0005476.1_g000003
rosa_roxburghii Rroxscaffold_3G00244590
rosa_rugosa Rorug07G0150200
rosa_samantha Rh7BG278800 Rh7CG307000 Rh7DG290600 Rh7DG397500
rosa_wichuraiana Rw7G024490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 440
AccI GTMKAC 2 cut(s) 280, 368
AciI CCGC 2 cut(s) 332, 422
AclWI GGATC 1 cut(s) 813
AcsI RAATTY 2 cut(s) 249, 934
AfeI AGCGCT 1 cut(s) 321
AfiI CCNNNNNNNGG 4 cut(s) 223, 451, 623, 921
AflII CTTAAG 2 cut(s) 872, 1055
AgsI TTSAA 5 cut(s) 244, 557, 571, 659, 738
AjnI CCWGG 1 cut(s) 345
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 4 cut(s) 438, 655, 857, 1054
AluI AGCT 4 cut(s) 438, 655, 857, 1054
Alw26I GTCTC 5 cut(s) 584, 649, 774, 868, 1010
AlwI GGATC 1 cut(s) 813
Ama87I CYCGRG 1 cut(s) 450
Aor51HI AGCGCT 1 cut(s) 321
ApeKI GCWGC 3 cut(s) 67, 610, 628
ApoI RAATTY 2 cut(s) 249, 934
Asp700I GAANNNNTTC 1 cut(s) 935
AspLEI GCGC 2 cut(s) 132, 322
AspS9I GGNCC 3 cut(s) 55, 343, 481
AsuC2I CCSGG 1 cut(s) 314
AsuHPI GGTGA 3 cut(s) 122, 215, 716
AvaI CYCGRG 1 cut(s) 450
AvaII GGWCC 3 cut(s) 55, 343, 481
BanII GRGCYC 1 cut(s) 1043
BarI GAAGNNNNNNTAC 2 cut(s) 521, 553
BbvI GCAGC 3 cut(s) 79, 622, 640
BccI CCATC 4 cut(s) 107, 269, 468, 980
BceAI ACGGC 3 cut(s) 80, 163, 435
BciT130I CCWGG 1 cut(s) 347
BcnI CCSGG 1 cut(s) 314
BcoDI GTCTC 5 cut(s) 584, 649, 774, 868, 1010
BfaI CTAG 2 cut(s) 471, 677
BfoI RGCGCY 2 cut(s) 133, 323
BfrI CTTAAG 2 cut(s) 872, 1055
BisI GCNGC 4 cut(s) 68, 423, 611, 629
BlsI GCNGC 4 cut(s) 69, 424, 612, 630
Bme1390I CCNGG 2 cut(s) 314, 347
Bme18I GGWCC 3 cut(s) 55, 343, 481
BmeT110I CYCGRG 1 cut(s) 450
BmgT120I GGNCC 3 cut(s) 55, 343, 481
BmiI GGNNCC 1 cut(s) 353
BmrFI CCNGG 2 cut(s) 314, 347
BmrI ACTGGG 1 cut(s) 147
BmsI GCATC 3 cut(s) 82, 269, 670
BmuI ACTGGG 1 cut(s) 147
BpmI CTGGAG 2 cut(s) 39, 764
BpuMI CCSGG 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 556
BsaI GGTCTC 2 cut(s) 584, 649
BsaJI CCNNGG 3 cut(s) 221, 346, 355
BsaWI WCCGGW 1 cut(s) 616
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 451, 623, 921
Bse1I ACTGG 1 cut(s) 142
Bse3DI GCAATG 2 cut(s) 131, 611
Bse8I GATNNNNATC 1 cut(s) 556
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 3 cut(s) 221, 346, 355
BseGI GGATG 3 cut(s) 280, 456, 587
BseJI GATNNNNATC 1 cut(s) 556
BseLI CCNNNNNNNGG 4 cut(s) 223, 451, 623, 921
BseMI GCAATG 2 cut(s) 131, 611
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 142
BseXI GCAGC 3 cut(s) 79, 622, 640
BseYI CCCAGC 2 cut(s) 322, 1007
BsiHKCI CYCGRG 1 cut(s) 450
BsiSI CCGG 3 cut(s) 313, 617, 773
BslI CCNNNNNNNGG 4 cut(s) 223, 451, 623, 921
BsmAI GTCTC 5 cut(s) 584, 649, 774, 868, 1010
BsmBI CGTCTC 2 cut(s) 774, 1010
Bso31I GGTCTC 2 cut(s) 584, 649
BsoBI CYCGRG 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 1043
Bsp143I GATC 3 cut(s) 287, 805, 883
Bsp19I CCATGG 1 cut(s) 355
BspACI CCGC 2 cut(s) 332, 422
BspCNI CTCAG 1 cut(s) 376
BspHI TCATGA 1 cut(s) 586
BspLI GGNNCC 1 cut(s) 353
BspPI GGATC 1 cut(s) 813
BspTI CTTAAG 2 cut(s) 872, 1055
BspTNI GGTCTC 2 cut(s) 584, 649
BsrDI GCAATG 2 cut(s) 131, 611
BsrI ACTGG 1 cut(s) 142
BssECI CCNNGG 3 cut(s) 221, 346, 355
BssMI GATC 3 cut(s) 287, 805, 883
BssNAI GTATAC 1 cut(s) 369
BssT1I CCWWGG 1 cut(s) 355
Bst1107I GTATAC 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 3 cut(s) 79, 222, 595
Bst6I CTCTTC 1 cut(s) 825
BstAFI CTTAAG 2 cut(s) 872, 1055
BstAPI GCANNNNNTGC 1 cut(s) 680
BstDEI CTNAG 3 cut(s) 191, 384, 755
BstDSI CCRYGG 2 cut(s) 221, 355
BstENI CCTNNNNNAGG 1 cut(s) 919
BstF5I GGATG 3 cut(s) 280, 456, 587
BstH2I RGCGCY 2 cut(s) 133, 323
BstHHI GCGC 2 cut(s) 132, 322
BstKTI GATC 3 cut(s) 290, 808, 886
BstMAI GTCTC 5 cut(s) 584, 649, 774, 868, 1010
BstMBI GATC 3 cut(s) 287, 805, 883
BstMWI GCNNNNNNNGC 4 cut(s) 422, 610, 680, 748
BstNI CCWGG 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 312, 345
BstV1I GCAGC 3 cut(s) 79, 622, 640
BstX2I RGATCY 1 cut(s) 805
BstXI CCANNNNNNTGG 1 cut(s) 305
BstYI RGATCY 1 cut(s) 805
BstZ17I GTATAC 1 cut(s) 369
BtgI CCRYGG 2 cut(s) 221, 355
BtsCI GGATG 3 cut(s) 280, 456, 587
BtsIMutI CAGTG 1 cut(s) 135
CciI TCATGA 1 cut(s) 586
CfoI GCGC 2 cut(s) 132, 322
Cfr13I GGNCC 3 cut(s) 55, 343, 481
CseI GACGC 1 cut(s) 602
CviAII CATG 5 cut(s) 356, 372, 426, 587, 867
DdeI CTNAG 3 cut(s) 191, 384, 755
DpnI GATC 3 cut(s) 289, 807, 885
DpnII GATC 3 cut(s) 287, 805, 883
DrdI GACNNNNNNGTC 1 cut(s) 440
DseDI GACNNNNNNGTC 1 cut(s) 440
Eam1104I CTCTTC 1 cut(s) 825
EarI CTCTTC 1 cut(s) 825
Eco130I CCWWGG 1 cut(s) 355
Eco24I GRGCYC 1 cut(s) 1043
Eco31I GGTCTC 2 cut(s) 584, 649
Eco32I GATATC 1 cut(s) 1048
Eco47I GGWCC 3 cut(s) 55, 343, 481
Eco47III AGCGCT 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 450
EcoNI CCTNNNNNAGG 1 cut(s) 919
EcoRII CCWGG 1 cut(s) 345
EcoRV GATATC 1 cut(s) 1048
EcoT14I CCWWGG 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 1043
ErhI CCWWGG 1 cut(s) 355
Esp3I CGTCTC 2 cut(s) 774, 1010
FaeI CATG 5 cut(s) 359, 375, 429, 590, 870
FatI CATG 5 cut(s) 355, 371, 425, 586, 866
FblI GTMKAC 2 cut(s) 280, 368
Fnu4HI GCNGC 4 cut(s) 68, 423, 611, 629
FokI GGATG 3 cut(s) 287, 443, 594
FriOI GRGCYC 1 cut(s) 1043
Fsp4HI GCNGC 4 cut(s) 68, 423, 611, 629
FspBI CTAG 2 cut(s) 471, 677
GlaI GCGC 2 cut(s) 131, 321
GluI GCNGC 4 cut(s) 68, 423, 611, 629
GsaI CCCAGC 2 cut(s) 326, 1011
GsuI CTGGAG 2 cut(s) 39, 764
HaeII RGCGCY 2 cut(s) 133, 323
HapII CCGG 3 cut(s) 313, 617, 773
HgaI GACGC 1 cut(s) 602
HhaI GCGC 2 cut(s) 132, 322
Hin1II CATG 5 cut(s) 359, 375, 429, 590, 870
Hin6I GCGC 2 cut(s) 130, 320
HinP1I GCGC 2 cut(s) 130, 320
HincII GTYRAC 2 cut(s) 689, 851
HindII GTYRAC 2 cut(s) 689, 851
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 6 cut(s) 176, 380, 557, 730, 847, 1031
HpaII CCGG 3 cut(s) 313, 617, 773
HphI GGTGA 3 cut(s) 122, 215, 716
Hpy166II GTNNAC 6 cut(s) 281, 343, 369, 445, 689, 851
Hpy188I TCNGA 7 cut(s) 265, 292, 385, 433, 481, 829, 909
Hpy188III TCNNGA 3 cut(s) 526, 587, 646
Hpy8I GTNNAC 6 cut(s) 281, 343, 369, 445, 689, 851
HpyAV CCTTC 4 cut(s) 46, 81, 101, 385
HpyCH4III ACNGT 3 cut(s) 79, 222, 595
HpyCH4IV ACGT 1 cut(s) 1019
HpyCH4V TGCA 3 cut(s) 95, 124, 674
HpyF10VI GCNNNNNNNGC 4 cut(s) 422, 610, 680, 748
HpyF3I CTNAG 3 cut(s) 191, 384, 755
HpySE526I ACGT 1 cut(s) 1019
Hsp92II CATG 5 cut(s) 359, 375, 429, 590, 870
HspAI GCGC 2 cut(s) 130, 320
Kzo9I GATC 3 cut(s) 287, 805, 883
Lsp1109I GCAGC 3 cut(s) 79, 622, 640
LweI GCATC 3 cut(s) 82, 269, 670
MaeI CTAG 2 cut(s) 471, 677
MaeII ACGT 1 cut(s) 1019
MaeIII GTNAC 2 cut(s) 377, 887
MalI GATC 3 cut(s) 289, 807, 885
MboI GATC 3 cut(s) 287, 805, 883
MboII GAAGA 2 cut(s) 842, 989
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 1 cut(s) 1043
MluCI AATT 6 cut(s) 249, 269, 335, 836, 934, 996
MlyI GAGTC 2 cut(s) 374, 856
MmeI TCCRAC 3 cut(s) 381, 459, 622
MnlI CCTC 9 cut(s) 14, 97, 477, 571, 739, 826, 871, 925, 952
MroXI GAANNNNTTC 1 cut(s) 935
MseI TTAA 3 cut(s) 873, 1022, 1056
MspCI CTTAAG 2 cut(s) 872, 1055
MspI CCGG 3 cut(s) 313, 617, 773
MspR9I CCNGG 2 cut(s) 314, 347
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 4 cut(s) 422, 610, 680, 748
NciI CCSGG 1 cut(s) 314
NcoI CCATGG 1 cut(s) 355
NdeII GATC 3 cut(s) 287, 805, 883
NlaIII CATG 5 cut(s) 359, 375, 429, 590, 870
NlaIV GGNNCC 1 cut(s) 353
NmuCI GTSAC 1 cut(s) 377
PagI TCATGA 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 935
PfeI GAWTC 4 cut(s) 176, 557, 730, 1031
PfoI TCCNGGA 1 cut(s) 312
PkrI GCNGC 4 cut(s) 69, 424, 612, 630
PleI GAGTC 2 cut(s) 374, 855
PpsI GAGTC 2 cut(s) 374, 855
Psp6I CCWGG 1 cut(s) 345
PspFI CCCAGC 2 cut(s) 322, 1007
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 1 cut(s) 353
PspPI GGNCC 3 cut(s) 55, 343, 481
PsuI RGATCY 1 cut(s) 805
SaqAI TTAA 3 cut(s) 873, 1022, 1056
SatI GCNGC 4 cut(s) 68, 423, 611, 629
Sau3AI GATC 3 cut(s) 287, 805, 883
Sau96I GGNCC 3 cut(s) 55, 343, 481
SchI GAGTC 2 cut(s) 374, 856
ScrFI CCNGG 2 cut(s) 314, 347
SduI GDGCHC 1 cut(s) 1043
SfaNI GCATC 3 cut(s) 82, 269, 670
SinI GGWCC 3 cut(s) 55, 343, 481
SmlI CTYRAG 2 cut(s) 872, 1055
SmoI CTYRAG 2 cut(s) 872, 1055
Sse9I AATT 6 cut(s) 249, 269, 335, 836, 934, 996
SsiI CCGC 2 cut(s) 332, 422
SspMI CTAG 2 cut(s) 471, 677
StyD4I CCNGG 2 cut(s) 312, 345
StyI CCWWGG 1 cut(s) 355
TaaI ACNGT 3 cut(s) 79, 222, 595
TaiI ACGT 1 cut(s) 1022
TaqI TCGA 3 cut(s) 550, 647, 1050
TaqII GACCGA 1 cut(s) 298
TasI AATT 6 cut(s) 249, 269, 335, 836, 934, 996
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 4 cut(s) 176, 557, 730, 1031
Tru1I TTAA 3 cut(s) 873, 1022, 1056
Tru9I TTAA 3 cut(s) 873, 1022, 1056
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 1 cut(s) 377
TseI GCWGC 3 cut(s) 67, 610, 628
Tsp45I GTSAC 1 cut(s) 377
TspDTI ATGAA 5 cut(s) 65, 71, 404, 893, 928
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 1 cut(s) 142
Vha464I CTTAAG 2 cut(s) 872, 1055
VpaK11BI GGWCC 3 cut(s) 55, 343, 481
XagI CCTNNNNNAGG 1 cut(s) 919
XapI RAATTY 2 cut(s) 249, 934
XmiI GTMKAC 2 cut(s) 280, 368
XmnI GAANNNNTTC 1 cut(s) 935
XspI CTAG 2 cut(s) 471, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.