pycom10g25550

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011534
Physical Location & Seq
Forward (+)
27143027 .. 27146430
3404 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1920 bp
ATGCAGACGAGGTACATGGAGAGATCCAATAGTATGGCGAGGGAGAAGCGAAGTTTGGATTCTTCTTCAGCTGAAGAAGGCCAGCCGGATAGGAAACGTCCTGCTCTCGCTAGTGTAATTGTTGAAGCTCTCAAGGTGGATAGTCTGCAAAAGCTTTGCTCGTCTTTGGAGCCGATTCTACGTAGAGTTGTTAGCGAAGAGGTGGAGCGTGCTTTAGCAAAATTAGGCCCTGCCAAACTTACTGGAAGGTCTTCTCCTAAGCAAATTGAAGGGCCCGATGGAAGAAACTTGCAGCTGCATTTTAGGTCCAGGTTGTCCCTTCCTCTTTTTACTGGTGGAAAAGTAGAAGGGGAGTGGGGTTCTGCAATCCCTATTGTCTTGATTGATGCAAATACAAAACACATCGTGACATCAGGTCCGGAGTCTGCGGTGAAATTAGACATTGTTGTGCTTGAAGGTGATTTTAACAACGAGGATGATGAGAACTGGACGCAAGAAGAATTTGAGAGCCATGTGGTGAAAGAGCGTGAAGGAAAGAGGCCACTTCTTACTGGGGATCTGCAAATTACACTGAACGAAGGTGTAGGAACACTAGGGGAATTGACATTTACCGATAACTCTAGCTGGATTAGGAGCAGGAAGTTTAGGCTAGGACTGAAAGTTGCTTCAGGCAATTGCGACAACATTCGTATACGGGAAGCAAAAACAGAAGCCATCACTGTTAAGGATCACCGTGGGGAATTGTACAAGAAACACTACCCGCCTGCGTTACATGATGAGGTCTGGAGATTAGAGAAAATTGGAAAAGATGGATCATTCCACAAGAGGCTGAATAAAGCTGGAATTGTTACAGTTGAAGACTTCCTACGACTTGTGGTTAGAGACTCGCAGAGACTGCGGAATATTCTTGGAAGTGGCATGTCAAATAAGATGTGGGATGGACTCATACAGCATGCAAAAACTTGTCTTCTTGGTGGGAAACTCTATGTCTATTATCCTGAGGATGCAAGGAATGTTGGTGTTGTTTTTAACAATATCTACGAGTTGAGTGGCCTAATTACCGATGAACAATTTTACTCTGCCGATTCTCTCTCGGAAACTCAGAAGGTCTATGTGGACGGTTTGGTAAAAAAGGCATACGACAACTGGATGCATGTTATGGAGTATGATGGAAAATCTCTTCTAAGCGTTAAGCAGCAGAAAAGTCCAGATGTTTCTCTACCTGAGGTCCCAATGGCCTCGCAAGATTATCCAAACTCATGTGATCAGCAGTTCACCCTACCTAGCCTGCTAGCTTCAGTTTCTTCACAACCGCCTCCTATGGATTCCGGCCTAAACGTGGGAGGTTATGATGCTGGCATGGCTGCCAGATTCCCAATACAGTCACAGAATGGAAATCTGAATGCTCCAGTTCAGCTCGATGACTTGTCATTTCCTCTACAGAATCAGCTGCCTAGCATTTCACACCATACGCATTTTCAAAGAAATGAAAACATGCTTTCCCTTGGTCCACTACAGTCGTCCACATCAGGGTTCCAGAATATCGGCACATCCAATCTATCTTCTTATAGGGGAGTCGAGGACCTCTTCCCAGAGGAAGAAATTCGTATGAGGAGCCATGAGATTCTCGAAAATGAAGATATGCAGCATCTTCTTCGTGTCTTTAACATGGACGGTCAGGGTCAAGGCCACAGTTATGGTCATTCCTCCATGAATGTCACTGAAGACAATTATCCTTATTCGTCACCATACATCCCCACTCCACAAGCGGACTACAGTATCGATGATGATCGGAGCCGTTCATCTGGGAAAGCTGTTGTTGGCTGGCTCAAGCTCAAGGCAGCCCTTAGATGGGGCATATTCGTCAGAAAGAAGGCTGCTGAGAGACGGGCTCAGCTTGTTGAGTTGGATGATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

640

Amino Acids

71.59

Weight (kDa)

5.89

Isoelectric Point (pI)

56.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 97 - 244 4.2e-62 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 256 - 322 1.2e-32 Calmodulin binding protein central domain
Calmod_bind_C PF20452 327 - 389 2.6e-23 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 691
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 5 cut(s) 428, 761, 898, 1313, 1769
AclWI GGATC 4 cut(s) 18, 564, 735, 820
AcsI RAATTY 2 cut(s) 500, 1602
AcuI CTGAAG 5 cut(s) 51, 93, 651, 1281, 1743
AdeI CACNNNGTG 1 cut(s) 406
AfaI GTAC 2 cut(s) 14, 746
AfiI CCNNNNNNNGG 4 cut(s) 1339, 1530, 1851, 1852
AgsI TTSAA 5 cut(s) 125, 269, 455, 857, 1481
AhdI GACNNNNNGTC 1 cut(s) 414
AjnI CCWGG 1 cut(s) 308
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
Alw26I GTCTC 3 cut(s) 876, 886, 1879
AlwI GGATC 4 cut(s) 18, 564, 735, 820
AlwNI CAGNNNCTG 1 cut(s) 895
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 8 cut(s) 79, 226, 272, 539, 1051, 1236, 1330, 1687
ApaI GGGCCC 1 cut(s) 276
ApeKI GCWGC 8 cut(s) 292, 295, 1195, 1364, 1450, 1645, 1841, 1877
ApoI RAATTY 2 cut(s) 500, 1602
Asp700I GAANNNNTTC 2 cut(s) 250, 1602
AspS9I GGNCC 8 cut(s) 227, 272, 273, 306, 416, 1228, 1508, 1582
AsuHPI GGTGA 6 cut(s) 442, 470, 529, 722, 1267, 1737
AsuNHI GCTAGC 1 cut(s) 1291
AvaII GGWCC 5 cut(s) 306, 416, 1228, 1508, 1582
AxyI CCTNAGG 2 cut(s) 999, 1224
BaeGI GKGCMC 1 cut(s) 276
BaeI ACNNNNGTAYC 2 cut(s) 1762, 1795
BanII GRGCYC 2 cut(s) 276, 1894
BarI GAAGNNNNNNTAC 2 cut(s) 849, 881
BbsI GAAGAC 4 cut(s) 243, 864, 959, 1731
BbvI GCAGC 8 cut(s) 282, 304, 1207, 1351, 1437, 1657, 1853, 1864
BccI CCATC 6 cut(s) 272, 722, 803, 932, 1163, 1845
BceAI ACGGC 1 cut(s) 1782
BcgI CGANNNNNNTGC 2 cut(s) 1637, 1671
BciT130I CCWGG 1 cut(s) 310
BclI TGATCA 1 cut(s) 1264
BcoDI GTCTC 3 cut(s) 876, 886, 1879
BfaI CTAG 7 cut(s) 111, 593, 621, 650, 1284, 1292, 1455
BfmI CTRYAG 3 cut(s) 1439, 1514, 1774
BisI GCNGC 8 cut(s) 293, 296, 1196, 1365, 1451, 1646, 1842, 1878
BlpI GCTNAGC 1 cut(s) 1893
BlsI GCNGC 8 cut(s) 294, 297, 1197, 1366, 1452, 1647, 1843, 1879
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 5 cut(s) 306, 416, 1228, 1508, 1582
BmeRI GACNNNNNGTC 1 cut(s) 414
BmgT120I GGNCC 8 cut(s) 227, 272, 273, 306, 416, 1228, 1508, 1582
BmiI GGNNCC 6 cut(s) 171, 274, 1230, 1535, 1616, 1796
BmrFI CCNGG 1 cut(s) 310
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 5 cut(s) 376, 994, 1140, 1342, 1657
BmtI GCTAGC 1 cut(s) 1295
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 4 cut(s) 243, 864, 959, 1731
BplI GAGNNNNNCTC 2 cut(s) 1876, 1908
BpmI CTGGAG 2 cut(s) 805, 1392
Bpu10I CCTNAGC 1 cut(s) 258
Bpu1102I GCTNAGC 1 cut(s) 1893
BpuEI CTTGAG 3 cut(s) 116, 1814, 1820
Bsa29I ATCGAT 1 cut(s) 1782
BsaAI YACGTR 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 1788
BsaJI CCNNGG 2 cut(s) 733, 1504
BsaWI WCCGGW 1 cut(s) 418
Bsc4I CCNNNNNNNGG 4 cut(s) 1339, 1530, 1851, 1852
Bse1I ACTGG 6 cut(s) 247, 337, 491, 556, 1151, 1409
Bse21I CCTNAGG 2 cut(s) 999, 1224
Bse8I GATNNNNATC 1 cut(s) 1788
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 1 cut(s) 310
BseCI ATCGAT 1 cut(s) 1782
BseDI CCNNGG 2 cut(s) 733, 1504
BseGI GGATG 7 cut(s) 481, 943, 1009, 1155, 1550, 1752, 1915
BseJI GATNNNNATC 1 cut(s) 1788
BseLI CCNNNNNNNGG 4 cut(s) 1339, 1530, 1851, 1852
BseMII CTCAG 5 cut(s) 990, 1115, 1215, 1872, 1907
BseNI ACTGG 6 cut(s) 247, 337, 491, 556, 1151, 1409
BseRI GAGGAG 1 cut(s) 1627
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 8 cut(s) 282, 304, 1207, 1351, 1437, 1657, 1853, 1864
BshFI GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1238, 1332, 1689
BshVI ATCGAT 1 cut(s) 1782
BsiSI CCGG 3 cut(s) 86, 419, 1329
BslFI GGGAC 2 cut(s) 301, 1214
BslI CCNNNNNNNGG 4 cut(s) 1339, 1530, 1851, 1852
BsmAI GTCTC 3 cut(s) 876, 886, 1879
BsmBI CGTCTC 1 cut(s) 1879
BsmFI GGGAC 2 cut(s) 301, 1214
BsmI GAATGC 1 cut(s) 1408
BsnI GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1238, 1332, 1689
Bsp120I GGGCCC 1 cut(s) 272
Bsp1286I GDGCHC 2 cut(s) 276, 1894
Bsp13I TCCGGA 1 cut(s) 418
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 6 cut(s) 23, 556, 727, 812, 1264, 1789
Bsp1720I GCTNAGC 1 cut(s) 1893
BspACI CCGC 5 cut(s) 428, 761, 898, 1313, 1769
BspANI GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1238, 1332, 1689
BspCNI CTCAG 5 cut(s) 991, 1114, 1216, 1873, 1906
BspDI ATCGAT 1 cut(s) 1782
BspEI TCCGGA 1 cut(s) 418
BspLI GGNNCC 6 cut(s) 171, 274, 1230, 1535, 1616, 1796
BspOI GCTAGC 1 cut(s) 1295
BspPI GGATC 4 cut(s) 18, 564, 735, 820
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 6 cut(s) 247, 337, 491, 556, 1151, 1409
BssECI CCNNGG 2 cut(s) 733, 1504
BssMI GATC 6 cut(s) 23, 556, 727, 812, 1264, 1789
BssNAI GTATAC 1 cut(s) 692
BssT1I CCWWGG 1 cut(s) 1504
Bst1107I GTATAC 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 9 cut(s) 721, 734, 853, 1121, 1383, 1518, 1676, 1694, 1778
Bst6I CTCTTC 3 cut(s) 192, 1185, 1592
BstAPI GCANNNNNTGC 1 cut(s) 895
BstAUI TGTACA 1 cut(s) 744
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 8 cut(s) 83, 210, 765, 954, 1289, 1293, 1357, 1826
BstDEI CTNAG 8 cut(s) 258, 999, 1101, 1184, 1224, 1847, 1881, 1893
BstDSI CCRYGG 1 cut(s) 733
BstF5I GGATG 7 cut(s) 481, 943, 1009, 1155, 1550, 1752, 1915
BstKTI GATC 6 cut(s) 26, 559, 730, 815, 1267, 1792
BstMAI GTCTC 3 cut(s) 876, 886, 1879
BstMBI GATC 6 cut(s) 23, 556, 727, 812, 1264, 1789
BstMWI GCNNNNNNNGC 2 cut(s) 895, 1361
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 4 cut(s) 922, 956, 1157, 1498
BstSCI CCNGG 1 cut(s) 308
BstSFI CTRYAG 3 cut(s) 1439, 1514, 1774
BstSLI GKGCMC 1 cut(s) 276
BstSNI TACGTA 1 cut(s) 182
BstV1I GCAGC 8 cut(s) 282, 304, 1207, 1351, 1437, 1657, 1853, 1864
BstV2I GAAGAC 4 cut(s) 243, 864, 959, 1731
BstX2I RGATCY 2 cut(s) 23, 556
BstXI CCANNNNNNTGG 2 cut(s) 34, 1697
BstYI RGATCY 2 cut(s) 23, 556
BstZ17I GTATAC 1 cut(s) 692
Bsu15I ATCGAT 1 cut(s) 1782
Bsu36I CCTNAGG 2 cut(s) 999, 1224
BsuRI GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1238, 1332, 1689
BsuTUI ATCGAT 1 cut(s) 1782
BtgI CCRYGG 1 cut(s) 733
BtsCI GGATG 7 cut(s) 481, 943, 1009, 1155, 1550, 1752, 1915
BtsIMutI CAGTG 3 cut(s) 569, 717, 1719
Cac8I GCNNGC 8 cut(s) 83, 210, 765, 954, 1289, 1293, 1357, 1826
CaiI CAGNNNCTG 1 cut(s) 895
Cfr13I GGNCC 8 cut(s) 227, 272, 273, 306, 416, 1228, 1508, 1582
ClaI ATCGAT 1 cut(s) 1782
CseI GACGC 1 cut(s) 499
Csp6I GTAC 2 cut(s) 13, 745
CviQI GTAC 2 cut(s) 13, 745
DdeI CTNAG 8 cut(s) 258, 999, 1101, 1184, 1224, 1847, 1881, 1893
DpnI GATC 6 cut(s) 25, 558, 729, 814, 1266, 1791
DpnII GATC 6 cut(s) 23, 556, 727, 812, 1264, 1789
DraIII CACNNNGTG 1 cut(s) 406
DriI GACNNNNNGTC 1 cut(s) 414
Eam1104I CTCTTC 3 cut(s) 192, 1185, 1592
Eam1105I GACNNNNNGTC 1 cut(s) 414
EarI CTCTTC 3 cut(s) 192, 1185, 1592
Eco105I TACGTA 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 1504
Eco24I GRGCYC 2 cut(s) 276, 1894
Eco47I GGWCC 5 cut(s) 306, 416, 1228, 1508, 1582
Eco57I CTGAAG 5 cut(s) 51, 93, 651, 1281, 1743
Eco81I CCTNAGG 2 cut(s) 999, 1224
EcoO109I RGGNCCY 4 cut(s) 227, 272, 1228, 1582
EcoRII CCWGG 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 1504
EcoT22I ATGCAT 1 cut(s) 1155
EcoT38I GRGCYC 2 cut(s) 276, 1894
ErhI CCWWGG 1 cut(s) 1504
Esp3I CGTCTC 1 cut(s) 1879
FaqI GGGAC 2 cut(s) 301, 1214
FauI CCCGC 1 cut(s) 768
FbaI TGATCA 1 cut(s) 1264
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 8 cut(s) 293, 296, 1196, 1365, 1451, 1646, 1842, 1878
FokI GGATG 6 cut(s) 488, 950, 1016, 1162, 1537, 1739
FriOI GRGCYC 2 cut(s) 276, 1894
Fsp4HI GCNGC 8 cut(s) 293, 296, 1196, 1365, 1451, 1646, 1842, 1878
FspBI CTAG 7 cut(s) 111, 593, 621, 650, 1284, 1292, 1455
GluI GCNGC 8 cut(s) 293, 296, 1196, 1365, 1451, 1646, 1842, 1878
GsuI CTGGAG 2 cut(s) 805, 1392
HaeIII GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1238, 1332, 1689
HapII CCGG 3 cut(s) 86, 419, 1329
HgaI GACGC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 152
HpaII CCGG 3 cut(s) 86, 419, 1329
HphI GGTGA 6 cut(s) 442, 470, 529, 722, 1267, 1737
Hpy166II GTNNAC 5 cut(s) 692, 1117, 1275, 1511, 1524
Hpy188I TCNGA 5 cut(s) 1096, 1104, 1401, 1794, 1868
Hpy188III TCNNGA 8 cut(s) 379, 406, 419, 784, 998, 1208, 1537, 1628
Hpy8I GTNNAC 5 cut(s) 692, 1117, 1275, 1511, 1524
HpyCH4III ACNGT 9 cut(s) 721, 734, 853, 1121, 1383, 1518, 1676, 1694, 1778
HpyCH4IV ACGT 3 cut(s) 97, 181, 1338
HpyF10VI GCNNNNNNNGC 2 cut(s) 895, 1361
HpyF3I CTNAG 8 cut(s) 258, 999, 1101, 1184, 1224, 1847, 1881, 1893
HpySE526I ACGT 3 cut(s) 97, 181, 1338
Kpn2I TCCGGA 1 cut(s) 418
Ksp22I TGATCA 1 cut(s) 1264
Kzo9I GATC 6 cut(s) 23, 556, 727, 812, 1264, 1789
LmnI GCTCC 6 cut(s) 169, 205, 633, 1411, 1614, 1794
Lsp1109I GCAGC 8 cut(s) 282, 304, 1207, 1351, 1437, 1657, 1853, 1864
LweI GCATC 5 cut(s) 376, 994, 1140, 1342, 1657
MaeI CTAG 7 cut(s) 111, 593, 621, 650, 1284, 1292, 1455
MaeII ACGT 3 cut(s) 97, 181, 1338
MaeIII GTNAC 6 cut(s) 406, 768, 847, 1383, 1717, 1743
MalI GATC 6 cut(s) 25, 558, 729, 814, 1266, 1791
MboI GATC 6 cut(s) 23, 556, 727, 812, 1264, 1789
MfeI CAATTG 1 cut(s) 673
MflI RGATCY 2 cut(s) 23, 556
MhlI GDGCHC 2 cut(s) 276, 1894
MlyI GAGTC 4 cut(s) 431, 878, 936, 1584
MmeI TCCRAC 1 cut(s) 1887
Mph1103I ATGCAT 1 cut(s) 1155
MroI TCCGGA 1 cut(s) 418
MroXI GAANNNNTTC 2 cut(s) 250, 1602
MseI TTAA 5 cut(s) 465, 723, 1029, 1191, 1665
MslI CAYNNNNRTG 2 cut(s) 446, 1695
MspA1I CMGCKG 3 cut(s) 71, 295, 1450
MspI CCGG 3 cut(s) 86, 419, 1329
MspR9I CCNGG 1 cut(s) 310
MunI CAATTG 1 cut(s) 673
Mva1269I GAATGC 1 cut(s) 1408
MvaI CCWGG 1 cut(s) 310
MwoI GCNNNNNNNGC 2 cut(s) 895, 1361
NdeII GATC 6 cut(s) 23, 556, 727, 812, 1264, 1789
NheI GCTAGC 1 cut(s) 1291
NlaIV GGNNCC 6 cut(s) 171, 274, 1230, 1535, 1616, 1796
NmuCI GTSAC 4 cut(s) 406, 1383, 1717, 1743
NsiI ATGCAT 1 cut(s) 1155
NspI RCATGY 4 cut(s) 922, 956, 1157, 1498
PaeI GCATGC 1 cut(s) 956
PctI GAATGC 1 cut(s) 1408
PdmI GAANNNNTTC 2 cut(s) 250, 1602
PfeI GAWTC 7 cut(s) 59, 175, 1085, 1325, 1371, 1444, 1624
PkrI GCNGC 8 cut(s) 294, 297, 1197, 1366, 1452, 1647, 1843, 1879
PleI GAGTC 4 cut(s) 430, 878, 936, 1583
PpsI GAGTC 4 cut(s) 430, 878, 936, 1583
Ppu21I YACGTR 1 cut(s) 182
PpuMI RGGWCCY 2 cut(s) 1228, 1582
Psp5II RGGWCCY 2 cut(s) 1228, 1582
Psp6I CCWGG 1 cut(s) 308
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 6 cut(s) 171, 274, 1230, 1535, 1616, 1796
PspOMI GGGCCC 1 cut(s) 272
PspPI GGNCC 8 cut(s) 227, 272, 273, 306, 416, 1228, 1508, 1582
PspPPI RGGWCCY 2 cut(s) 1228, 1582
PstNI CAGNNNCTG 1 cut(s) 895
PsuI RGATCY 2 cut(s) 23, 556
PvuII CAGCTG 3 cut(s) 71, 295, 1450
RsaI GTAC 2 cut(s) 14, 746
RsaNI GTAC 2 cut(s) 13, 745
RseI CAYNNNNRTG 2 cut(s) 446, 1695
SaqAI TTAA 5 cut(s) 465, 723, 1029, 1191, 1665
SatI GCNGC 8 cut(s) 293, 296, 1196, 1365, 1451, 1646, 1842, 1878
Sau3AI GATC 6 cut(s) 23, 556, 727, 812, 1264, 1789
Sau96I GGNCC 8 cut(s) 227, 272, 273, 306, 416, 1228, 1508, 1582
SchI GAGTC 4 cut(s) 431, 878, 936, 1584
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 2 cut(s) 276, 1894
SfaNI GCATC 5 cut(s) 376, 994, 1140, 1342, 1657
SfcI CTRYAG 3 cut(s) 1439, 1514, 1774
SinI GGWCC 5 cut(s) 306, 416, 1228, 1508, 1582
SmiMI CAYNNNNRTG 2 cut(s) 446, 1695
SmlI CTYRAG 3 cut(s) 131, 1829, 1835
SmoI CTYRAG 3 cut(s) 131, 1829, 1835
SnaBI TACGTA 1 cut(s) 182
SphI GCATGC 1 cut(s) 956
SsiI CCGC 5 cut(s) 428, 761, 898, 1313, 1769
SspI AATATT 1 cut(s) 904
SspMI CTAG 7 cut(s) 111, 593, 621, 650, 1284, 1292, 1455
StyD4I CCNGG 1 cut(s) 308
StyI CCWWGG 1 cut(s) 1504
TaaI ACNGT 9 cut(s) 721, 734, 853, 1121, 1383, 1518, 1676, 1694, 1778
TaiI ACGT 3 cut(s) 100, 184, 1341
TaqI TCGA 4 cut(s) 1419, 1578, 1629, 1782
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 7 cut(s) 59, 175, 1085, 1325, 1371, 1444, 1624
Tru1I TTAA 5 cut(s) 465, 723, 1029, 1191, 1665
Tru9I TTAA 5 cut(s) 465, 723, 1029, 1191, 1665
TscAI CASTG 3 cut(s) 576, 724, 1726
TseFI GTSAC 4 cut(s) 406, 1383, 1717, 1743
TseI GCWGC 8 cut(s) 292, 295, 1195, 1364, 1450, 1645, 1841, 1877
Tsp45I GTSAC 4 cut(s) 406, 1383, 1717, 1743
TspDTI ATGAA 5 cut(s) 1080, 1503, 1650, 1727, 1791
TspRI CASTG 3 cut(s) 576, 724, 1726
VpaK11BI GGWCC 5 cut(s) 306, 416, 1228, 1508, 1582
XapI RAATTY 2 cut(s) 500, 1602
XceI RCATGY 4 cut(s) 922, 956, 1157, 1498
XmiI GTMKAC 1 cut(s) 691
XmnI GAANNNNTTC 2 cut(s) 250, 1602
XspI CTAG 7 cut(s) 111, 593, 621, 650, 1284, 1292, 1455
Zsp2I ATGCAT 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.