pycom10g26250

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014095
Physical Location & Seq
Forward (+)
27635120 .. 27636123
1004 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
ATGGTTAAAAACGCGACTCCTCTCGTCTGCCTCTCGGTCTTGATTATGGACGTCGTGGCGGGAATCCTTGGGATTCAAGCTGAGATTGCTCAAAACAAGGTGCACCATTTGAAGATGTGGATCTTTGAGTGCAGGGACCCAAGTTACCAGGCCTTCAAATTAGGGTTGGCAGCTGCTGTGATACTAGCAGTTGCACATGTAATAGGCAACTTGCTTGGTGGCTGCATTTGCATCTCGTCCAAGGAAGATTACCAAAAAGCCACAGCAAATAAGCAATTAGCAGTTGCTTCCCTCATCTTATCATGGATTACATTGGCGGTGGCATTTTCACTGCTGATTGCGGGGACAATGTCAAACAGAAAGTCGAGAAAATCGTGCGGGTTGTCACACCGTCGGGTTCTGTCCATCGGAGGGATCTTATGCTTCTTCCATGGATTGTTCATCATTGCATATTACGTCTCTGCCAAAGCCACGTTCAAAGAAGGGAAACTCAAACCCACCACACCCGGCAACCCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

174

Amino Acids

18.57

Weight (kDa)

9.63

Isoelectric Point (pI)

31.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 145 1.2e-23 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014095)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21310 AT4G21310
fragaria_vesca FvH4_3g02950
malus_domestica MD10G1311300.v1.1
prunus_persica Prupe.4G029600_v2.0.a1
pyrus_communis pycom10g26250
rosa_chinensis RchiOBHm_Chr5g0004561
rosa_laevigata RLG00000031240
rosa_multiflora Rmu_sc0002354.1_g000018 Rmu_sc0003758.1_g000012
rosa_roxburghii Rroxscaffold_1G00070970
rosa_rugosa Rorug04G0412200
rosa_samantha Rh5AG040200 Rh5BG039600 Rh5CG043500 Rh5DG039300
rosa_wichuraiana Rw5G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 54
AccII CGCG 1 cut(s) 14
AciI CCGC 4 cut(s) 59, 317, 341, 378
AclWI GGATC 2 cut(s) 128, 422
AcyI GRCGYC 1 cut(s) 51
AfiI CCNNNNNNNGG 1 cut(s) 411
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 4 cut(s) 77, 112, 157, 478
AjnI CCWGG 1 cut(s) 147
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AluBI AGCT 3 cut(s) 80, 173, 518
AluI AGCT 3 cut(s) 80, 173, 518
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 463
Alw44I GTGCAC 1 cut(s) 101
AlwI GGATC 2 cut(s) 128, 422
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 1 cut(s) 150
ApaLI GTGCAC 1 cut(s) 101
ApeKI GCWGC 3 cut(s) 170, 173, 222
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspS9I GGNCC 1 cut(s) 136
AsuC2I CCSGG 1 cut(s) 507
AvaII GGWCC 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 3 cut(s) 160, 182, 209
BccI CCATC 1 cut(s) 413
BciT130I CCWGG 1 cut(s) 149
BcnI CCSGG 1 cut(s) 507
BcoDI GTCTC 1 cut(s) 463
BfaI CTAG 1 cut(s) 185
BisI GCNGC 3 cut(s) 171, 174, 223
BlsI GCNGC 3 cut(s) 172, 175, 224
Bme1390I CCNGG 2 cut(s) 149, 507
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 2 cut(s) 137, 138
BmrFI CCNGG 2 cut(s) 149, 507
BmsI GCATC 1 cut(s) 240
BpuMI CCSGG 1 cut(s) 507
BsaBI GATNNNNATC 1 cut(s) 119
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 3 cut(s) 67, 240, 430
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse3DI GCAATG 1 cut(s) 444
Bse8I GATNNNNATC 1 cut(s) 119
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 3 cut(s) 67, 240, 430
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 411
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 9
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 3 cut(s) 160, 182, 209
BseYI CCCAGC 1 cut(s) 514
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 1 cut(s) 507
BslFI GGGAC 2 cut(s) 149, 358
BslI CCNNNNNNNGG 1 cut(s) 411
BsmAI GTCTC 1 cut(s) 463
BsmBI CGTCTC 1 cut(s) 463
BsmFI GGGAC 2 cut(s) 149, 358
BsnI GGCC 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 2 cut(s) 120, 414
Bsp19I CCATGG 1 cut(s) 430
BspACI CCGC 4 cut(s) 59, 317, 341, 378
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 2 cut(s) 137, 138
BspPI GGATC 2 cut(s) 128, 422
BsrDI GCAATG 1 cut(s) 444
BssECI CCNNGG 3 cut(s) 67, 240, 430
BssMI GATC 2 cut(s) 120, 414
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 3 cut(s) 67, 240, 430
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 392
BstACI GRCGYC 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 81
BstDSI CCRYGG 1 cut(s) 430
BstFNI CGCG 1 cut(s) 14
BstKTI GATC 2 cut(s) 123, 417
BstMAI GTCTC 1 cut(s) 463
BstMBI GATC 2 cut(s) 120, 414
BstMWI GCNNNNNNNGC 2 cut(s) 86, 228
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 2 cut(s) 147, 505
BstSLI GKGCMC 1 cut(s) 105
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 3 cut(s) 160, 182, 209
BstX2I RGATCY 2 cut(s) 120, 414
BstYI RGATCY 2 cut(s) 120, 414
BsuRI GGCC 1 cut(s) 152
BtgI CCRYGG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 329
BtsIMutI CAGTG 1 cut(s) 329
CaiI CAGNNNCTG 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 136
CviAII CATG 3 cut(s) 197, 303, 431
CviJI RGCY 7 cut(s) 80, 152, 173, 222, 260, 470, 518
CviKI_1 RGCY 7 cut(s) 80, 152, 173, 222, 260, 470, 518
DdeI CTNAG 1 cut(s) 81
DpnI GATC 2 cut(s) 122, 416
DpnII GATC 2 cut(s) 120, 414
Eco130I CCWWGG 3 cut(s) 67, 240, 430
Eco147I AGGCCT 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 147
EcoT14I CCWWGG 3 cut(s) 67, 240, 430
ErhI CCWWGG 3 cut(s) 67, 240, 430
Esp3I CGTCTC 1 cut(s) 463
FaeI CATG 3 cut(s) 200, 306, 434
FaiI YATR 6 cut(s) 47, 198, 304, 421, 432, 451
FaqI GGGAC 2 cut(s) 149, 358
FatI CATG 3 cut(s) 196, 302, 430
FauI CCCGC 3 cut(s) 52, 334, 371
Fnu4HI GCNGC 3 cut(s) 171, 174, 223
Fsp4HI GCNGC 3 cut(s) 171, 174, 223
FspBI CTAG 1 cut(s) 185
GluI GCNGC 3 cut(s) 171, 174, 223
GsaI CCCAGC 1 cut(s) 518
HaeIII GGCC 1 cut(s) 152
HapII CCGG 1 cut(s) 507
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 3 cut(s) 200, 306, 434
HinfI GANTC 3 cut(s) 16, 63, 73
HpaII CCGG 1 cut(s) 507
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 1 cut(s) 410
Hpy188III TCNNGA 2 cut(s) 40, 366
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 2 cut(s) 56, 396
HpyAV CCTTC 2 cut(s) 163, 476
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 3 cut(s) 51, 456, 473
HpyCH4V TGCA 6 cut(s) 103, 132, 194, 225, 231, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 228
HpyF3I CTNAG 1 cut(s) 81
HpySE526I ACGT 3 cut(s) 51, 456, 473
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 3 cut(s) 200, 306, 434
KflI GGGWCCC 1 cut(s) 136
Kzo9I GATC 2 cut(s) 120, 414
LpnPI CCDG 3 cut(s) 118, 134, 161
Lsp1109I GCAGC 3 cut(s) 160, 182, 209
LweI GCATC 1 cut(s) 240
MaeI CTAG 1 cut(s) 185
MaeII ACGT 3 cut(s) 51, 456, 473
MaeIII GTNAC 2 cut(s) 143, 384
MalI GATC 2 cut(s) 122, 416
MboI GATC 2 cut(s) 120, 414
MboII GAAGA 3 cut(s) 124, 257, 418
MflI RGATCY 2 cut(s) 120, 414
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 2 cut(s) 158, 275
MlyI GAGTC 1 cut(s) 10
MnlI CCTC 4 cut(s) 30, 41, 302, 404
MseI TTAA 1 cut(s) 6
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 2 cut(s) 149, 507
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 2 cut(s) 86, 228
NciI CCSGG 1 cut(s) 507
NcoI CCATGG 1 cut(s) 430
NdeII GATC 2 cut(s) 120, 414
NlaIII CATG 3 cut(s) 200, 306, 434
NlaIV GGNNCC 2 cut(s) 137, 138
NmuCI GTSAC 1 cut(s) 384
NspI RCATGY 1 cut(s) 200
PceI AGGCCT 1 cut(s) 152
PciI ACATGT 1 cut(s) 196
PcsI WCGNNNNNNNCGW 1 cut(s) 371
PfeI GAWTC 2 cut(s) 63, 73
PflFI GACNNNGTC 1 cut(s) 349
PkrI GCNGC 3 cut(s) 172, 175, 224
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
PpuMI RGGWCCY 1 cut(s) 136
PscI ACATGT 1 cut(s) 196
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 514
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 137, 138
PspPI GGNCC 1 cut(s) 136
PspPPI RGGWCCY 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 2 cut(s) 120, 414
PsyI GACNNNGTC 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 173
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 3 cut(s) 171, 174, 223
Sau3AI GATC 2 cut(s) 120, 414
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 10
ScrFI CCNGG 2 cut(s) 149, 507
SduI GDGCHC 1 cut(s) 105
SetI ASST 7 cut(s) 54, 82, 102, 175, 459, 476, 520
SfaNI GCATC 1 cut(s) 240
SinI GGWCC 1 cut(s) 136
Sse9I AATT 2 cut(s) 158, 275
SseBI AGGCCT 1 cut(s) 152
SsiI CCGC 4 cut(s) 59, 317, 341, 378
SspMI CTAG 1 cut(s) 185
StuI AGGCCT 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 147, 505
StyI CCWWGG 3 cut(s) 67, 240, 430
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 3 cut(s) 54, 459, 476
TaqI TCGA 1 cut(s) 365
TaqII GACCGA 1 cut(s) 25
TasI AATT 2 cut(s) 158, 275
TfiI GAWTC 2 cut(s) 63, 73
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 1 cut(s) 384
TseI GCWGC 3 cut(s) 170, 173, 222
Tsp45I GTSAC 1 cut(s) 384
TspDTI ATGAA 1 cut(s) 430
TspRI CASTG 1 cut(s) 336
Tth111I GACNNNGTC 1 cut(s) 349
VneI GTGCAC 1 cut(s) 101
VpaK11BI GGWCC 1 cut(s) 136
XceI RCATGY 1 cut(s) 200
XspI CTAG 1 cut(s) 185
ZraI GACGTC 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.