pycom10g29040

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008724
Physical Location & Seq
Forward (+)
29470391 .. 29472255
1865 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1623 bp
ATGTGTGCTAAGTTCTGTTACGGAATTACGATTAATCTCAGCGCCTATAATTTGGTACCTGCATTTTGTGCTGCTAAGTTCCTCCGAATGACCGAGTCAGTAGAGAAGGGAAATTTTGTTCCAAAACTCGAGGCTTTCTTCAATTCATGCGTTCTTGAAGGTTGGAAAGACTCCATTACTGCATTACAAACTACAGTGAAGTTGCCTGAGTGGTCTGAGAATCTTGGGATCATCAGAAAGTGCATTGATTCAATTGTAGAGAAAATCCTTACTCCTCCAGCAAAGGTTGCGTGGTCCTACACTTATACCAGACCTGGCTACACCAAAAAGCAACATCATTCTGTCCCAAAGGATTGGTGGACTGAGGATATATCAGATCTTGATGTAGACCTGTTTCGATGTATAATTACTGCTGTTAGAGCCACGCATGTGCTGCCACCACAGCTCATTGGGGAAGCTTTGCACGTCTATGCTTGTCGTTGGCTGCCAGATACCACAAGAACACCTCCACAAATAGATGAGGAACTCATGGAAAACAATCGAAGAATTGTTGATAGCATTGTGAGTATGATTCCCGGAGATAAGGGAGCAGTTTCTGTTGGTTTCTTGCTAAGGCTTACTGTAATTGCCAATTACTTGAGAGTGTCCCCAGTGACAAAGACTGACCTGTTAAGGAGGTCTGGTCTACAACTCGAAGAGGCAACGGTGGATGACCTGATTTTTCCTTCAAACTCGCCTGCTGACCCGGAATTTTACGATATTGACTTGGTTTTGGAAGTGTTAGAAAGTTTCTTGGTGCTATGGAGAAGACAATCCCCTGCAGCTGCTTCTGAATTGGACAGAAGTGCTCAGGTTTTAGGAACAATGAGAAAGGTTGGGAAACTCATTGATTCGTACCTTCAAGTTGTTGCCAGGGATGTCAACATGCCAGTTTCAAAAGTGGTATCTCTGGCTGAAGCTTTGCCAGATATTGCAAGGGAAGACCATGACGGCATCTACAAGGCTATTAACATTTATCTAAAGGAGCATGGTGATCTAAGCAAGGCAGACAAGAAACGCTTGTGCCGCATTTTAGACTGCCAGAAGTTGTCGCCTGAGGTACGCACTCATGCTGTGAAAAATGAGCGGCTACCACTGAGGACTGTTGTGCAAGTCCTCTTCTTTGAACAAGAGAGAGACAGAGGTTCCAACTCCAAGTCAGCAGCAGCAACTCATGATCGTCATAAACATAAGCCACTGCCAATAACGCCCTCCCAGTCCCATGAGCCAGGAAAACAAACAACGCCAAGTAGCAGAGAATCGGATAGTCATAGTCATAAGCTAAAATTGGGAGCAGCAAATGTTCATGATAAATTCAGTAGTAGTACCACTGGAAAGAAAGATAATCCACTGCTGCAAATGCAAAACAAAAGATCAGGCGGAAAGTTGGCATTGAAAAACGAAAGAAAGGGTGTCAGATTAGAAACGCAACAAGTAGATCATCAAGGACATAATATGGGAAGGGAAATCGTAAGAGGAGGAGCATCTGGATCTACAGGTGCAGGGAGCAAGTTGGACGCTAAGAAGATGATCCAAAGGGGAAGTAGGCCGGACCATGGCCACGACAAAGGCAAAGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

541

Amino Acids

60.39

Weight (kDa)

9.1

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 118 - 366 1.2e-79 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 67
Acc65I GGTACC 1 cut(s) 55
AccB1I GGYRCC 1 cut(s) 55
AccBSI CCGCTC 1 cut(s) 1126
AccI GTMKAC 2 cut(s) 387, 685
AciI CCGC 3 cut(s) 1066, 1126, 1419
AclWI GGATC 3 cut(s) 236, 1537, 1564
AcoI YGGCCR 1 cut(s) 1597
AcsI RAATTY 3 cut(s) 112, 749, 1352
AcuI CTGAAG 1 cut(s) 975
AfaI GTAC 4 cut(s) 57, 896, 1101, 1366
AfiI CCNNNNNNNGG 1 cut(s) 1595
AgsI TTSAA 8 cut(s) 142, 158, 252, 729, 902, 936, 1166, 1435
AjiI CACGTC 1 cut(s) 466
AjnI CCWGG 3 cut(s) 313, 911, 1267
AleI CACNNNNGTG 1 cut(s) 428
AloI GAACNNNNNNTCC 2 cut(s) 102, 134
AluBI AGCT 5 cut(s) 445, 458, 824, 959, 1321
AluI AGCT 5 cut(s) 445, 458, 824, 959, 1321
Alw21I GWGCWC 1 cut(s) 850
Alw26I GTCTC 1 cut(s) 1170
AlwI GGATC 3 cut(s) 236, 1537, 1564
AlwNI CAGNNNCTG 1 cut(s) 596
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 2 cut(s) 1586, 1597
ApeKI GCWGC 9 cut(s) 71, 433, 484, 821, 824, 1202, 1205, 1334, 1393
ApoI RAATTY 3 cut(s) 112, 749, 1352
AseI ATTAAT 1 cut(s) 33
Asp718I GGTACC 1 cut(s) 55
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 2 cut(s) 294, 1591
AsuC2I CCSGG 2 cut(s) 576, 746
AsuHPI GGTGA 1 cut(s) 1043
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 2 cut(s) 294, 1591
AxyI CCTNAGG 1 cut(s) 1095
BalI TGGCCA 1 cut(s) 1599
BanI GGYRCC 1 cut(s) 55
BbsI GAAGAC 2 cut(s) 814, 987
Bbv12I GWGCWC 1 cut(s) 850
BbvI GCAGC 9 cut(s) 58, 420, 471, 811, 833, 1214, 1217, 1346, 1380
BceAI ACGGC 1 cut(s) 1006
BciT130I CCWGG 3 cut(s) 315, 913, 1269
BcnI CCSGG 2 cut(s) 576, 746
BcoDI GTCTC 1 cut(s) 1170
BfmI CTRYAG 3 cut(s) 192, 819, 1533
BfoI RGCGCY 1 cut(s) 45
BfuAI ACCTGC 1 cut(s) 67
BglII AGATCT 1 cut(s) 376
Bme1390I CCNGG 5 cut(s) 315, 576, 746, 913, 1269
Bme18I GGWCC 2 cut(s) 294, 1591
BmeT110I CYCGRG 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 466
BmgT120I GGNCC 2 cut(s) 294, 1591
BmiI GGNNCC 2 cut(s) 57, 1186
BmrFI CCNGG 5 cut(s) 315, 576, 746, 913, 1269
BmrI ACTGGG 2 cut(s) 644, 1249
BmsI GCATC 2 cut(s) 1002, 1532
BmuI ACTGGG 2 cut(s) 644, 1249
BpiI GAAGAC 2 cut(s) 814, 987
BpmI CTGGAG 1 cut(s) 261
Bpu10I CCTNAGC 2 cut(s) 611, 849
BpuEI CTTGAG 1 cut(s) 658
BpuMI CCSGG 2 cut(s) 576, 746
BsaJI CCNNGG 2 cut(s) 912, 1594
BsaXI ACNNNNNCTCC 2 cut(s) 667, 697
Bsc4I CCNNNNNNNGG 1 cut(s) 1595
Bse1I ACTGG 4 cut(s) 650, 929, 1255, 1375
Bse21I CCTNAGG 1 cut(s) 1095
BseBI CCWGG 3 cut(s) 315, 913, 1269
BseDI CCNNGG 2 cut(s) 912, 1594
BseGI GGATG 2 cut(s) 715, 922
BseLI CCNNNNNNNGG 1 cut(s) 1595
BseMII CTCAG 7 cut(s) 52, 198, 207, 354, 863, 1086, 1127
BseNI ACTGG 4 cut(s) 650, 929, 1255, 1375
BseRI GAGGAG 3 cut(s) 264, 1530, 1533
BseXI GCAGC 9 cut(s) 58, 420, 471, 811, 833, 1214, 1217, 1346, 1380
BsgI GTGCAG 1 cut(s) 1560
BshFI GGCC 2 cut(s) 1588, 1599
BshNI GGYRCC 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 850
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 3 cut(s) 576, 746, 1589
BslFI GGGAC 3 cut(s) 329, 631, 1243
BslI CCNNNNNNNGG 1 cut(s) 1595
BsmAI GTCTC 1 cut(s) 1170
BsmFI GGGAC 3 cut(s) 329, 631, 1243
BsnI GGCC 2 cut(s) 1588, 1599
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 850
Bsp143I GATC 8 cut(s) 228, 376, 1033, 1216, 1412, 1477, 1529, 1569
Bsp19I CCATGG 1 cut(s) 1594
BspACI CCGC 3 cut(s) 1066, 1126, 1419
BspANI GGCC 2 cut(s) 1588, 1599
BspCNI CTCAG 7 cut(s) 51, 199, 208, 355, 862, 1087, 1128
BspHI TCATGA 2 cut(s) 1213, 1345
BspLI GGNNCC 2 cut(s) 57, 1186
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 67
BspPI GGATC 3 cut(s) 236, 1537, 1564
BspT107I GGYRCC 1 cut(s) 55
BsrBI CCGCTC 1 cut(s) 1126
BsrI ACTGG 4 cut(s) 650, 929, 1255, 1375
BssECI CCNNGG 2 cut(s) 912, 1594
BssMI GATC 8 cut(s) 228, 376, 1033, 1216, 1412, 1477, 1529, 1569
BssT1I CCWWGG 1 cut(s) 1594
Bst2UI CCWGG 3 cut(s) 315, 913, 1269
Bst4CI ACNGT 4 cut(s) 196, 622, 706, 1144
Bst6I CTCTTC 2 cut(s) 690, 1163
BstAPI GCANNNNNTGC 3 cut(s) 68, 287, 433
BstC8I GCNNGC 1 cut(s) 738
BstDSI CCRYGG 1 cut(s) 1594
BstF5I GGATG 2 cut(s) 715, 922
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 8 cut(s) 231, 379, 1036, 1219, 1415, 1480, 1532, 1572
BstMAI GTCTC 1 cut(s) 1170
BstMBI GATC 8 cut(s) 228, 376, 1033, 1216, 1412, 1477, 1529, 1569
BstMWI GCNNNNNNNGC 8 cut(s) 68, 287, 419, 433, 442, 1065, 1246, 1399
BstNI CCWGG 3 cut(s) 315, 913, 1269
BstNSI RCATGY 2 cut(s) 431, 928
BstSCI CCNGG 5 cut(s) 313, 574, 744, 911, 1267
BstSFI CTRYAG 3 cut(s) 192, 819, 1533
BstV1I GCAGC 9 cut(s) 58, 420, 471, 811, 833, 1214, 1217, 1346, 1380
BstV2I GAAGAC 2 cut(s) 814, 987
BstX2I RGATCY 2 cut(s) 376, 1529
BstXI CCANNNNNNTGG 1 cut(s) 354
BstYI RGATCY 2 cut(s) 376, 1529
Bsu36I CCTNAGG 1 cut(s) 1095
BsuRI GGCC 2 cut(s) 1588, 1599
BtgI CCRYGG 1 cut(s) 1594
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 2 cut(s) 715, 922
BtsI GCAGTG 2 cut(s) 1235, 1388
BtsIMutI CAGTG 6 cut(s) 201, 657, 1133, 1235, 1368, 1388
BveI ACCTGC 1 cut(s) 67
Cac8I GCNNGC 1 cut(s) 738
CaiI CAGNNNCTG 1 cut(s) 596
CciI TCATGA 2 cut(s) 1213, 1345
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 2 cut(s) 294, 1591
CseI GACGC 1 cut(s) 1565
Csp6I GTAC 4 cut(s) 56, 895, 1100, 1365
CviQI GTAC 4 cut(s) 56, 895, 1100, 1365
DpnI GATC 8 cut(s) 230, 378, 1035, 1218, 1414, 1479, 1531, 1571
DpnII GATC 8 cut(s) 228, 376, 1033, 1216, 1412, 1477, 1529, 1569
EaeI YGGCCR 1 cut(s) 1597
Eam1104I CTCTTC 2 cut(s) 690, 1163
EarI CTCTTC 2 cut(s) 690, 1163
EciI GGCGGA 1 cut(s) 1434
Eco130I CCWWGG 1 cut(s) 1594
Eco47I GGWCC 2 cut(s) 294, 1591
Eco57I CTGAAG 1 cut(s) 975
Eco81I CCTNAGG 1 cut(s) 1095
Eco88I CYCGRG 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 313, 911, 1267
EcoT14I CCWWGG 1 cut(s) 1594
ErhI CCWWGG 1 cut(s) 1594
FalI AAGNNNNNCTT 2 cut(s) 1043, 1075
FaqI GGGAC 3 cut(s) 329, 631, 1243
FblI GTMKAC 2 cut(s) 387, 685
FokI GGATG 2 cut(s) 722, 929
GlaI GCGC 1 cut(s) 43
GsuI CTGGAG 1 cut(s) 261
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 2 cut(s) 1588, 1599
HapII CCGG 3 cut(s) 576, 746, 1589
HgaI GACGC 1 cut(s) 1565
HhaI GCGC 1 cut(s) 44
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HindIII AAGCTT 2 cut(s) 456, 957
HinfI GANTC 7 cut(s) 95, 170, 220, 248, 571, 890, 1298
HpaII CCGG 3 cut(s) 576, 746, 1589
HphI GGTGA 1 cut(s) 1043
Hpy166II GTNNAC 4 cut(s) 360, 388, 686, 922
Hpy188I TCNGA 7 cut(s) 86, 217, 236, 376, 832, 1303, 1457
Hpy188III TCNNGA 5 cut(s) 155, 380, 1214, 1346, 1527
Hpy8I GTNNAC 4 cut(s) 360, 388, 686, 922
HpyAV CCTTC 5 cut(s) 100, 152, 735, 908, 1494
HpyCH4III ACNGT 4 cut(s) 196, 622, 706, 1144
HpyCH4IV ACGT 1 cut(s) 465
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 287, 419, 433, 442, 1065, 1246, 1399
HpySE526I ACGT 1 cut(s) 465
HspAI GCGC 1 cut(s) 42
KpnI GGTACC 1 cut(s) 59
Kzo9I GATC 8 cut(s) 228, 376, 1033, 1216, 1412, 1477, 1529, 1569
LmnI GCTCC 5 cut(s) 587, 1024, 1331, 1520, 1545
Lsp1109I GCAGC 9 cut(s) 58, 420, 471, 811, 833, 1214, 1217, 1346, 1380
LweI GCATC 2 cut(s) 1002, 1532
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 2 cut(s) 17, 652
MalI GATC 8 cut(s) 230, 378, 1035, 1218, 1414, 1479, 1531, 1571
MbiI CCGCTC 1 cut(s) 1126
MboI GATC 8 cut(s) 228, 376, 1033, 1216, 1412, 1477, 1529, 1569
MboII GAAGA 7 cut(s) 130, 555, 707, 819, 992, 1150, 1576
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 2 cut(s) 376, 1529
MhlI GDGCHC 1 cut(s) 850
MlsI TGGCCA 1 cut(s) 1599
MluNI TGGCCA 1 cut(s) 1599
MlyI GAGTC 2 cut(s) 104, 164
MmeI TCCRAC 3 cut(s) 143, 1212, 1533
Mox20I TGGCCA 1 cut(s) 1599
MscI TGGCCA 1 cut(s) 1599
MseI TTAA 3 cut(s) 33, 671, 1008
MslI CAYNNNNRTG 2 cut(s) 428, 468
Msp20I TGGCCA 1 cut(s) 1599
MspA1I CMGCKG 1 cut(s) 824
MspI CCGG 3 cut(s) 576, 746, 1589
MspR9I CCNGG 5 cut(s) 315, 576, 746, 913, 1269
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 3 cut(s) 315, 913, 1269
MwoI GCNNNNNNNGC 8 cut(s) 68, 287, 419, 433, 442, 1065, 1246, 1399
NciI CCSGG 2 cut(s) 576, 746
NcoI CCATGG 1 cut(s) 1594
NdeII GATC 8 cut(s) 228, 376, 1033, 1216, 1412, 1477, 1529, 1569
NlaIV GGNNCC 2 cut(s) 57, 1186
NmuCI GTSAC 1 cut(s) 652
NspI RCATGY 2 cut(s) 431, 928
OliI CACNNNNGTG 1 cut(s) 428
PaeR7I CTCGAG 1 cut(s) 128
PagI TCATGA 2 cut(s) 1213, 1345
PfeI GAWTC 5 cut(s) 220, 248, 571, 890, 1298
PflFI GACNNNGTC 1 cut(s) 94
PfoI TCCNGGA 1 cut(s) 574
PleI GAGTC 2 cut(s) 103, 164
PpsI GAGTC 2 cut(s) 103, 164
PshBI ATTAAT 1 cut(s) 33
Psp6I CCWGG 3 cut(s) 313, 911, 1267
PspGI CCWGG 3 cut(s) 313, 911, 1267
PspN4I GGNNCC 2 cut(s) 57, 1186
PspPI GGNCC 2 cut(s) 294, 1591
PspXI VCTCGAGB 1 cut(s) 128
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 596
PsuI RGATCY 2 cut(s) 376, 1529
PsyI GACNNNGTC 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 824
RsaI GTAC 4 cut(s) 57, 896, 1101, 1366
RsaNI GTAC 4 cut(s) 56, 895, 1100, 1365
RseI CAYNNNNRTG 2 cut(s) 428, 468
SaqAI TTAA 3 cut(s) 33, 671, 1008
Sau3AI GATC 8 cut(s) 228, 376, 1033, 1216, 1412, 1477, 1529, 1569
Sau96I GGNCC 2 cut(s) 294, 1591
SchI GAGTC 2 cut(s) 104, 164
ScrFI CCNGG 5 cut(s) 315, 576, 746, 913, 1269
SduI GDGCHC 1 cut(s) 850
SfaNI GCATC 2 cut(s) 1002, 1532
SfcI CTRYAG 3 cut(s) 192, 819, 1533
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 2 cut(s) 294, 1591
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 2 cut(s) 428, 468
SmlI CTYRAG 2 cut(s) 128, 637
SmoI CTYRAG 2 cut(s) 128, 637
SsiI CCGC 3 cut(s) 1066, 1126, 1419
StyD4I CCNGG 5 cut(s) 313, 574, 744, 911, 1267
StyI CCWWGG 1 cut(s) 1594
TaaI ACNGT 4 cut(s) 196, 622, 706, 1144
TaiI ACGT 1 cut(s) 468
TaqI TCGA 4 cut(s) 129, 397, 541, 693
TaqII GACCGA 1 cut(s) 107
TauI GCSGC 2 cut(s) 1068, 1129
TfiI GAWTC 5 cut(s) 220, 248, 571, 890, 1298
Tru1I TTAA 3 cut(s) 33, 671, 1008
Tru9I TTAA 3 cut(s) 33, 671, 1008
TscAI CASTG 6 cut(s) 201, 657, 1140, 1242, 1375, 1395
TseFI GTSAC 1 cut(s) 652
TseI GCWGC 9 cut(s) 71, 433, 484, 821, 824, 1202, 1205, 1334, 1393
Tsp45I GTSAC 1 cut(s) 652
TspDTI ATGAA 2 cut(s) 135, 1334
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 6 cut(s) 201, 657, 1140, 1242, 1375, 1395
Tth111I GACNNNGTC 1 cut(s) 94
VpaK11BI GGWCC 2 cut(s) 294, 1591
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 3 cut(s) 112, 749, 1352
XceI RCATGY 2 cut(s) 431, 928
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XhoI CTCGAG 1 cut(s) 128
XmiI GTMKAC 2 cut(s) 387, 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.