pycom10g29060

Domain of unknown function (DUF2431)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0016278
Physical Location & Seq
Forward (+)
29477662 .. 29478618
957 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGGGAAAAAAGGAAGAAGATTACAAGGAACAGAGGGAAGCAGACGTAGAAACAGAAGAAGAAGAAGAAGACGAAGAAGAATCGGATTCAGAAGAGAAATGGAGAAAGCACTATTCGTCTGGGCAGAGGATATTGCTGGTGGGCGAAGGAGACTTCTCCTTCTCTCTTTCTTTGGCCACTCGCTTTGGATCTGCCCGCAACATGGTTGCCACTTCTCTCGATTCCCAGGAGAAGATCGCTGGCAAGTACAGTAACGCCATAGAGAATGTAAGGAAGCTTGAAGAAAGGGGATGCATCGTGATTTACGGAGTGGACGCCCAGCTTATGAGCCAACACTTCTTCCTCGCCACTCAGAGGTTTGACCGCATCGTCTACAACTTCCCTCATGTTGGCTTTCTCTTCCGCGAGGATAGCTTCTGTCAGATCCAGTTGAACAAGAGATTGGTGAAGGGTTTCTTCAAAAATGCCAAATTACTCCTGAGAAAAGAGGGTGGAGGAGAGATTCACATAACCCACAAAGAGGGTCATCCTTACAACGAATGGGATTTGGTCAGGAAGGCACACAAGATTGGCCTGCAAATCGATCGATCCGTGCCCTTCTGCATGGACGACTATCCCGGCTACCACAACAAGAGAGGCCACGGCATCCATTCCGACGCCCCCTTCCACCTTGGCGATTGCACTACTTACATGTTCAAAATCAGGCTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

238

Amino Acids

27.53

Weight (kDa)

6.02

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 45 - 212 1.2e-55 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016278)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00200
malus_domestica MD10G1341000.v1.1
prunus_persica Prupe.4G002200_v2.0.a1
pyrus_communis pycom10g29060
rosa_chinensis RchiOBHm_Chr5g0000261
rosa_laevigata RLG00000030854
rosa_multiflora Rmu_sc0043092.1_g000001 Rmu_ssc0000385.1_g000003
rosa_roxburghii Rroxscaffold_1G00075840
rosa_rugosa Rorug04G0382900
rosa_samantha Rh5AG001900 Rh5BG003300 Rh5CG002200 Rh5DG002200
rosa_wichuraiana Rw5G000240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AccI GTMKAC 1 cut(s) 372
AccII CGCG 1 cut(s) 405
AciI CCGC 3 cut(s) 196, 364, 403
AclWI GGATC 3 cut(s) 196, 418, 582
AcoI YGGCCR 1 cut(s) 174
AcyI GRCGYC 2 cut(s) 315, 657
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 4 cut(s) 202, 354, 389, 520
AflIII ACRYGT 1 cut(s) 690
AgsI TTSAA 4 cut(s) 281, 433, 460, 697
AjnI CCWGG 1 cut(s) 225
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AluBI AGCT 3 cut(s) 277, 322, 414
AluI AGCT 3 cut(s) 277, 322, 414
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 3 cut(s) 196, 418, 582
AoxI GGCC 3 cut(s) 174, 571, 637
ApeKI GCWGC 1 cut(s) 706
Asp700I GAANNNNTTC 1 cut(s) 452
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 1 cut(s) 457
BaeGI GKGCMC 1 cut(s) 597
BalI TGGCCA 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 693
BceAI ACGGC 1 cut(s) 658
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BciT130I CCWGG 1 cut(s) 227
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 1 cut(s) 144
BisI GCNGC 1 cut(s) 707
BlsI GCNGC 1 cut(s) 708
Bme1390I CCNGG 2 cut(s) 227, 618
BmrFI CCNGG 2 cut(s) 227, 618
BmsI GCATC 4 cut(s) 281, 303, 375, 654
BpiI GAAGAC 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 618
Bsa29I ATCGAT 2 cut(s) 582, 586
BsaHI GRCGYC 2 cut(s) 315, 657
BsaJI CCNNGG 3 cut(s) 225, 640, 670
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 354, 389, 520
Bse1I ACTGG 1 cut(s) 427
BseBI CCWGG 1 cut(s) 227
BseCI ATCGAT 2 cut(s) 582, 586
BseDI CCNNGG 3 cut(s) 225, 640, 670
BseGI GGATG 3 cut(s) 296, 526, 645
BseLI CCNNNNNNNGG 4 cut(s) 202, 354, 389, 520
BseMII CTCAG 2 cut(s) 365, 470
BseNI ACTGG 1 cut(s) 427
BseRI GAGGAG 1 cut(s) 510
BseSI GKGCMC 1 cut(s) 597
BseXI GCAGC 1 cut(s) 693
BseYI CCCAGC 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 586
BshFI GGCC 3 cut(s) 176, 573, 639
BshVI ATCGAT 2 cut(s) 582, 586
BsiEI CGRYCG 1 cut(s) 586
BsiSI CCGG 1 cut(s) 618
BslI CCNNNNNNNGG 4 cut(s) 202, 354, 389, 520
BsmAI GTCTC 1 cut(s) 144
BsnI GGCC 3 cut(s) 176, 573, 639
Bsp1286I GDGCHC 1 cut(s) 597
Bsp143I GATC 5 cut(s) 188, 234, 423, 583, 587
BspACI CCGC 3 cut(s) 196, 364, 403
BspANI GGCC 3 cut(s) 176, 573, 639
BspCNI CTCAG 2 cut(s) 364, 471
BspDI ATCGAT 2 cut(s) 582, 586
BspFNI CGCG 1 cut(s) 405
BspPI GGATC 3 cut(s) 196, 418, 582
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 3 cut(s) 225, 640, 670
BssMI GATC 5 cut(s) 188, 234, 423, 583, 587
BssNI GRCGYC 2 cut(s) 315, 657
BssT1I CCWWGG 1 cut(s) 670
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 2 cut(s) 87, 404
BstACI GRCGYC 2 cut(s) 315, 657
BstC8I GCNNGC 3 cut(s) 196, 241, 575
BstDEI CTNAG 2 cut(s) 351, 479
BstDSI CCRYGG 1 cut(s) 640
BstF5I GGATG 3 cut(s) 296, 526, 645
BstFNI CGCG 1 cut(s) 405
BstKTI GATC 5 cut(s) 191, 237, 426, 586, 590
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 5 cut(s) 188, 234, 423, 583, 587
BstMCI CGRYCG 1 cut(s) 586
BstMWI GCNNNNNNNGC 1 cut(s) 411
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 694
BstSCI CCNGG 2 cut(s) 225, 616
BstSLI GKGCMC 1 cut(s) 597
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 693
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 2 cut(s) 188, 423
BstYI RGATCY 2 cut(s) 188, 423
Bsu15I ATCGAT 2 cut(s) 582, 586
BsuRI GGCC 3 cut(s) 176, 573, 639
BsuTUI ATCGAT 2 cut(s) 582, 586
BtgI CCRYGG 1 cut(s) 640
BtsCI GGATG 3 cut(s) 296, 526, 645
Cac8I GCNNGC 3 cut(s) 196, 241, 575
ClaI ATCGAT 2 cut(s) 582, 586
CseI GACGC 2 cut(s) 323, 665
Csp6I GTAC 1 cut(s) 247
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviAII CATG 4 cut(s) 202, 386, 604, 691
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 2 cut(s) 351, 479
DpnI GATC 5 cut(s) 190, 236, 425, 585, 589
DpnII GATC 5 cut(s) 188, 234, 423, 583, 587
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 2 cut(s) 87, 404
EarI CTCTTC 2 cut(s) 87, 404
Eco130I CCWWGG 1 cut(s) 670
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 670
EcoT22I ATGCAT 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 670
FaeI CATG 4 cut(s) 205, 389, 607, 694
FaiI YATR 7 cut(s) 203, 260, 326, 387, 509, 605, 692
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FatI CATG 4 cut(s) 201, 385, 603, 690
FauI CCCGC 1 cut(s) 203
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 707
FokI GGATG 3 cut(s) 303, 513, 632
Fsp4HI GCNGC 1 cut(s) 707
GluI GCNGC 1 cut(s) 707
GsaI CCCAGC 1 cut(s) 322
HaeIII GGCC 3 cut(s) 176, 573, 639
HapII CCGG 1 cut(s) 618
HgaI GACGC 2 cut(s) 323, 665
Hin1I GRCGYC 2 cut(s) 315, 657
Hin1II CATG 4 cut(s) 205, 389, 607, 694
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 4 cut(s) 80, 86, 221, 502
HpaII CCGG 1 cut(s) 618
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 2 cut(s) 313, 373
Hpy188I TCNGA 5 cut(s) 85, 91, 354, 423, 655
Hpy188III TCNNGA 4 cut(s) 218, 298, 478, 553
Hpy8I GTNNAC 2 cut(s) 313, 373
Hpy99I CGWCG 1 cut(s) 659
HpyAV CCTTC 6 cut(s) 140, 169, 442, 550, 607, 673
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 4 cut(s) 294, 577, 603, 681
HpyF10VI GCNNNNNNNGC 1 cut(s) 411
HpyF3I CTNAG 2 cut(s) 351, 479
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 2 cut(s) 315, 657
Hsp92II CATG 4 cut(s) 205, 389, 607, 694
Kzo9I GATC 5 cut(s) 188, 234, 423, 583, 587
Lsp1109I GCAGC 1 cut(s) 693
LweI GCATC 4 cut(s) 281, 303, 375, 654
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 251
MalI GATC 5 cut(s) 190, 236, 425, 585, 589
MboI GATC 5 cut(s) 188, 234, 423, 583, 587
MflI RGATCY 2 cut(s) 188, 423
MhlI GDGCHC 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 1 cut(s) 470
MluNI TGGCCA 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 678
Mox20I TGGCCA 1 cut(s) 176
Mph1103I ATGCAT 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 452
MscI TGGCCA 1 cut(s) 176
Msp20I TGGCCA 1 cut(s) 176
MspI CCGG 1 cut(s) 618
MspR9I CCNGG 2 cut(s) 227, 618
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 1 cut(s) 411
NciI CCSGG 1 cut(s) 618
NdeII GATC 5 cut(s) 188, 234, 423, 583, 587
NlaIII CATG 4 cut(s) 205, 389, 607, 694
NsiI ATGCAT 1 cut(s) 296
NspI RCATGY 1 cut(s) 694
PciI ACATGT 1 cut(s) 690
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 4 cut(s) 80, 86, 221, 502
PkrI GCNGC 1 cut(s) 708
Ple19I CGATCG 1 cut(s) 586
PscI ACATGT 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 318
PspGI CCWGG 1 cut(s) 225
PsuI RGATCY 2 cut(s) 188, 423
PvuI CGATCG 1 cut(s) 586
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SatI GCNGC 1 cut(s) 707
Sau3AI GATC 5 cut(s) 188, 234, 423, 583, 587
ScrFI CCNGG 2 cut(s) 227, 618
SduI GDGCHC 1 cut(s) 597
SetI ASST 6 cut(s) 48, 279, 324, 359, 416, 672
SfaNI GCATC 4 cut(s) 281, 303, 375, 654
Sse9I AATT 1 cut(s) 470
SsiI CCGC 3 cut(s) 196, 364, 403
StyD4I CCNGG 2 cut(s) 225, 616
StyI CCWWGG 1 cut(s) 670
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 1 cut(s) 48
TaqI TCGA 3 cut(s) 219, 582, 586
TasI AATT 1 cut(s) 470
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 4 cut(s) 80, 86, 221, 502
TseI GCWGC 1 cut(s) 706
TspGWI ACGGA 2 cut(s) 321, 580
XceI RCATGY 1 cut(s) 694
XmiI GTMKAC 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 452
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.