pycom111g00250

Ring finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0006714
Physical Location & Seq
Forward (+)
194908 .. 198765
3858 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2334 bp
ATGTCCATCTTGCCCAACCAAGACCAAGGATCTATATCGTCATCTGCCTCTTCAGCTCCCTCTGTAAACCCTAACTCCCAACATGGACACCGCCACCGCCACCGTCACGTTTCCCTTTCTCAATCTCAGCCCCAGTCGCTGTCTCCGCCGGATCCCTCCCGCCAAATTCTCACATTTGGTTCGCTTCGGATCTCCGACTCTCAATCTAGCTCCTCCGCTACTGGAGGCCTATCACCACCGGCACCAGCTGCAGAAGATTCTGGTGGATCTTCCCAGCAGGTGAATGAGTTAGGGATGCCCGGGGGTAACAAGACTTTACCTAATAACCACCACAATACACATTCACAGTGTCACTCTGGTGGAGGTGGACGAGGGGTTGGATCGCCCTGGTCTAGAGGGAAAAGATCAGGAACCTTTGGTTCTGGTTCAACTGAACAAAGTATCGGATCTGCTGCTTCTCATGGAAACTCCACTCATCAAACAGGAAGGAAAGCCCAGCTAATGAATGGCAACCACTTGTTGAATTTTTATTATGACCCCATAGCTCGTCCTCAGCGTAGAGCTCCTCCTCCTCCTCCAAGAAGGCAGCACAAGAGGAAGCCTTACAATAAAGATTTATTTCTTCAGGCTAACTACAAATTTGTGGTGTTAGATTCAGGAAACTACTCAGCTGAATCGATGGATCCAGATAAGATGTTACAATGGGAGGACATCATTTGCGTGAGATATTCCACCCCAACTGTGGTTCAATGTCCCATTTGTCTGGAATATCCTCTTTGTCCGCAGATAACCTCATGTGGACATATTTTCTGTTTCCCATGTATTCTGCAATACTTGCTGATGGGGAAGGAGGACCATAAAGGAGACTGCTGGAAAAGATGCCCGTTGTGTTTTGTTATGATATCACCTAAGGATTTGTACACTCTATTCGTGGAGAATGTCAAGCAGTATTGTGTTGGTGATACAATAGAGTTTATGCTTCTTAGTCGACAAAAGGACTCATTTACTTTATCCCATAAAAGTAAACAGGAGAAAGATGCTGTTGGAGGTTGTGATGATGAAAGCTATGATCCCTTTTCGAAGTTTACATTTACATCAGATGTAGATCTTGCAGTCAGAAAAGCGATATCAGAGTTAGATGGTTGGTTGGTCAGGGCAGAGTCGGGGCTTGTTGATGACATAGAGAAGCTTCCATATGTATGTGCTGCAATGGAACAATTAGAACAGAGGAAGAAATATTGGAACGAGCACTGGGTTAGCGACAGTAATAAATCTTGTATATATAATGATCATAATAGTGGTTCTATCTTGTCAACTGAAAATTCTGCGAATGGTAACACTGAAGCATCTACATTTGGACATGCAAATCCATCCAATGAAGTCTATGACCATAATAAGCGTTCAGACAATTTATCTCTGGAGAAGTCGGATGATGGGACTTGTTCTGACAACTCTGTGGATGCAGCTGAATCATTGGAAGGCCATGAAAATGTTTTGTCTTCTTCATATGATGAAAGCAAGAGTGCTCGAGGGGACTTACATGACTCTGGAATTCTAAAGGAGAAGGAATCATACAATTTCTACCAGGGAGCTGATGGTCAGCACATCATTCTTCATCCATTGAACATGAAATGCCTGCTACACCATTATGGGAGCCATGATATGCTACCACACAGGATAAGTGGAAAAATTTTGCAATTGGAGACAGTGACTCAGACAGAAGCTATGAGGAGGCGCTACCGCTACTTGAGTCATTTTTCTTTAACAACAATACTCCAGTTTTGTGAGATTGATCTGAGTGAGGTATTGTCTCCTGATGCCTTGTCTCCCTTTATGGAAGACATAAAGAAGCGTGAAAAGCAGAGGAAACAACTTGCTAGGAAGGAGCAAAGGGAGAAAATGAAGGCTGAATCTACTGTGGCATATCCCATCCCCATAGTAGCAGGTTATGGACAGTCCTTTCATGATGGATCCCCTACATTCTCCATGGATGACTTCGAGGCTTTGGGAAGTTCTACAGCGACATCTTCAAGCTCTCCTGTTGTTGGGGAAAGGAAGCTCTTCTCAAGTGTTACAAGGCTTGGTTTTGCTGCTGCGCACGATTCTCCTGCCCTGAAACTAGAAGGAACCAATTCTCTGAATGGCGATGATGCAGGGAGAGTCTCTCCCAAGACAAATGGTACCCAGAATGCGGGTGTTACGTCATTTGCTAATGTAATCTCCAGAGCTAAGCCTGGTGAAAATATGGACCCGCCCAAGATAAATGATTCGGGAAAGAAGGGAAGGAAACAGAGTCGAGTCCTTTTGTCAACAGCTGGTGGTCGACGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4

Protein Analysis

778

Amino Acids

85.57

Weight (kDa)

6.89

Isoelectric Point (pI)

59.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4 PF00097 251 - 297 6.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 268
Acc16I TGCGCA 1 cut(s) 2097
Acc36I ACCTGC 2 cut(s) 268, 1934
Acc65I GGTACC 1 cut(s) 2180
AccB1I GGYRCC 2 cut(s) 241, 2180
AccI GTMKAC 2 cut(s) 988, 2323
AciI CCGC 9 cut(s) 91, 97, 146, 160, 216, 782, 1741, 2192, 2252
AcsI RAATTY 6 cut(s) 165, 523, 638, 1321, 1551, 1689
AcuI CTGAAG 3 cut(s) 36, 608, 1362
AfaI GTAC 2 cut(s) 920, 2182
AfiI CCNNNNNNNGG 3 cut(s) 742, 2045, 2191
AgsI TTSAA 5 cut(s) 429, 523, 749, 1624, 2031
AjnI CCWGG 3 cut(s) 386, 1584, 2233
AleI CACNNNNGTG 1 cut(s) 357
Alw21I GWGCWC 3 cut(s) 565, 1251, 1528
Alw26I GTCTC 6 cut(s) 147, 858, 1697, 1815, 1830, 2167
AlwNI CAGNNNCTG 1 cut(s) 139
Ama87I CYCGRG 2 cut(s) 299, 1527
AoxI GGCC 2 cut(s) 226, 1480
ApeKI GCWGC 7 cut(s) 248, 452, 586, 1205, 1463, 2090, 2093
ApoI RAATTY 6 cut(s) 165, 523, 638, 1321, 1551, 1689
Asp700I GAANNNNTTC 2 cut(s) 2060, 2131
Asp718I GGTACC 1 cut(s) 2180
AspLEI GCGC 2 cut(s) 1737, 2098
AspS9I GGNCC 2 cut(s) 853, 2248
AsuC2I CCSGG 2 cut(s) 300, 301
AsuHPI GGTGA 5 cut(s) 225, 292, 897, 971, 2249
AsuII TTCGAA 1 cut(s) 1079
AvaI CYCGRG 2 cut(s) 299, 1527
AvaII GGWCC 2 cut(s) 853, 2248
AxyI CCTNAGG 1 cut(s) 909
BamHI GGATCC 3 cut(s) 151, 682, 1970
BanI GGYRCC 2 cut(s) 241, 2180
BanII GRGCYC 1 cut(s) 565
BarI GAAGNNNNNNTAC 2 cut(s) 1556, 1588
BbsI GAAGAC 2 cut(s) 1491, 1845
Bbv12I GWGCWC 3 cut(s) 565, 1251, 1528
BbvCI CCTCAGC 1 cut(s) 552
BbvI GCAGC 7 cut(s) 235, 439, 598, 1192, 1475, 2077, 2080
BccI CCATC 9 cut(s) 14, 673, 835, 1133, 1378, 1427, 1589, 1937, 1961
BcgI CGANNNNNNTGC 2 cut(s) 2090, 2124
BciT130I CCWGG 3 cut(s) 388, 1586, 2235
BclI TGATCA 1 cut(s) 1288
BcnI CCSGG 2 cut(s) 300, 301
BcoDI GTCTC 6 cut(s) 147, 858, 1697, 1815, 1830, 2167
BfaI CTAG 4 cut(s) 207, 393, 1878, 2120
BfmI CTRYAG 2 cut(s) 249, 2016
BfoI RGCGCY 1 cut(s) 1738
BfuAI ACCTGC 2 cut(s) 268, 1934
BglII AGATCT 1 cut(s) 1105
BisI GCNGC 7 cut(s) 249, 453, 587, 1206, 1464, 2091, 2094
BlpI GCTNAGC 1 cut(s) 2229
BlsI GCNGC 7 cut(s) 250, 454, 588, 1207, 1465, 2092, 2095
Bme1390I CCNGG 5 cut(s) 300, 301, 388, 1586, 2235
Bme18I GGWCC 2 cut(s) 853, 2248
BmeT110I CYCGRG 2 cut(s) 299, 1527
BmgT120I GGNCC 2 cut(s) 853, 2248
BmiI GGNNCC 9 cut(s) 153, 243, 412, 684, 1655, 1972, 2128, 2182, 2250
BmrFI CCNGG 5 cut(s) 300, 301, 388, 1586, 2235
BmrI ACTGGG 2 cut(s) 127, 1261
BmsI GCATC 7 cut(s) 285, 869, 1027, 1355, 1450, 1807, 2140
BmuI ACTGGG 2 cut(s) 127, 1261
BpiI GAAGAC 2 cut(s) 1491, 1845
BplI GAGNNNNNCTC 2 cut(s) 2149, 2181
BpmI CTGGAG 4 cut(s) 243, 1439, 1760, 2206
Bpu10I CCTNAGC 1 cut(s) 552
Bpu1102I GCTNAGC 1 cut(s) 2229
Bpu14I TTCGAA 1 cut(s) 1079
BpuEI CTTGAG 2 cut(s) 1768, 2050
BpuMI CCSGG 2 cut(s) 300, 301
Bsa29I ATCGAT 1 cut(s) 677
BsaBI GATNNNNATC 2 cut(s) 34, 1104
BsaJI CCNNGG 6 cut(s) 25, 299, 300, 386, 1585, 1986
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 3 cut(s) 742, 2045, 2191
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 4 cut(s) 133, 226, 1256, 1777
Bse21I CCTNAGG 1 cut(s) 909
Bse3DI GCAATG 1 cut(s) 1215
Bse8I GATNNNNATC 2 cut(s) 34, 1104
BseBI CCWGG 3 cut(s) 388, 1586, 2235
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 6 cut(s) 25, 299, 300, 386, 1585, 1986
BseGI GGATG 7 cut(s) 300, 1370, 1435, 1465, 1615, 1929, 1996
BseJI GATNNNNATC 2 cut(s) 34, 1104
BseLI CCNNNNNNNGG 3 cut(s) 742, 2045, 2191
BseMI GCAATG 1 cut(s) 1215
BseMII CTCAG 5 cut(s) 140, 566, 681, 1727, 1787
BseNI ACTGG 4 cut(s) 133, 226, 1256, 1777
BseRI GAGGAG 6 cut(s) 202, 555, 558, 561, 564, 1744
BseXI GCAGC 7 cut(s) 235, 439, 598, 1192, 1475, 2077, 2080
BseYI CCCAGC 2 cut(s) 273, 495
BshFI GGCC 2 cut(s) 228, 1482
BshNI GGYRCC 2 cut(s) 241, 2180
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 3 cut(s) 565, 1251, 1528
BsiHKCI CYCGRG 2 cut(s) 299, 1527
BsiSI CCGG 3 cut(s) 149, 239, 300
BslFI GGGAC 3 cut(s) 738, 1450, 1547
BslI CCNNNNNNNGG 3 cut(s) 742, 2045, 2191
BsmAI GTCTC 6 cut(s) 147, 858, 1697, 1815, 1830, 2167
BsmFI GGGAC 3 cut(s) 738, 1450, 1547
BsmI GAATGC 1 cut(s) 2194
BsnI GGCC 2 cut(s) 228, 1482
BsoBI CYCGRG 2 cut(s) 299, 1527
Bsp119I TTCGAA 1 cut(s) 1079
Bsp1286I GDGCHC 3 cut(s) 565, 1251, 1528
Bsp1407I TGTACA 1 cut(s) 918
Bsp1720I GCTNAGC 1 cut(s) 2229
Bsp19I CCATGG 1 cut(s) 1986
BspACI CCGC 9 cut(s) 91, 97, 146, 160, 216, 782, 1741, 2192, 2252
BspANI GGCC 2 cut(s) 228, 1482
BspCNI CTCAG 5 cut(s) 139, 565, 680, 1726, 1788
BspDI ATCGAT 1 cut(s) 677
BspHI TCATGA 1 cut(s) 1963
BspLI GGNNCC 9 cut(s) 153, 243, 412, 684, 1655, 1972, 2128, 2182, 2250
BspMAI CTGCAG 1 cut(s) 253
BspMI ACCTGC 2 cut(s) 268, 1934
BspQI GCTCTTC 1 cut(s) 2066
BspT104I TTCGAA 1 cut(s) 1079
BspT107I GGYRCC 2 cut(s) 241, 2180
BsrDI GCAATG 1 cut(s) 1215
BsrFI RCCGGY 1 cut(s) 238
BsrGI TGTACA 1 cut(s) 918
BsrI ACTGG 4 cut(s) 133, 226, 1256, 1777
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 6 cut(s) 25, 299, 300, 386, 1585, 1986
BssT1I CCWWGG 2 cut(s) 25, 1986
Bst2UI CCWGG 3 cut(s) 388, 1586, 2235
Bst4CI ACNGT 7 cut(s) 104, 348, 742, 1265, 1708, 1918, 1956
Bst6I CTCTTC 2 cut(s) 55, 2066
BstAPI GCANNNNNTGC 2 cut(s) 248, 835
BstAUI TGTACA 1 cut(s) 918
BstBI TTCGAA 1 cut(s) 1079
BstC8I GCNNGC 1 cut(s) 1637
BstDEI CTNAG 8 cut(s) 126, 552, 667, 909, 983, 1713, 1796, 2229
BstDSI CCRYGG 1 cut(s) 1986
BstF5I GGATG 7 cut(s) 300, 1370, 1435, 1465, 1615, 1929, 1996
BstH2I RGCGCY 1 cut(s) 1738
BstHHI GCGC 2 cut(s) 1737, 2098
BstMAI GTCTC 6 cut(s) 147, 858, 1697, 1815, 1830, 2167
BstMWI GCNNNNNNNGC 6 cut(s) 53, 136, 145, 248, 835, 1858
BstNI CCWGG 3 cut(s) 388, 1586, 2235
BstNSI RCATGY 1 cut(s) 1364
BstSCI CCNGG 5 cut(s) 298, 299, 386, 1584, 2233
BstSFI CTRYAG 2 cut(s) 249, 2016
BstV1I GCAGC 7 cut(s) 235, 439, 598, 1192, 1475, 2077, 2080
BstV2I GAAGAC 2 cut(s) 1491, 1845
BstX2I RGATCY 8 cut(s) 29, 151, 189, 266, 446, 682, 1105, 1970
BstXI CCANNNNNNTGG 1 cut(s) 763
BstYI RGATCY 8 cut(s) 29, 151, 189, 266, 446, 682, 1105, 1970
Bsu15I ATCGAT 1 cut(s) 677
Bsu36I CCTNAGG 1 cut(s) 909
BsuRI GGCC 2 cut(s) 228, 1482
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 1 cut(s) 1986
BtgZI GCGATG 1 cut(s) 2160
BtsCI GGATG 7 cut(s) 300, 1370, 1435, 1465, 1615, 1929, 1996
BtsIMutI CAGTG 4 cut(s) 353, 1249, 1338, 1713
BveI ACCTGC 2 cut(s) 268, 1934
Cac8I GCNNGC 1 cut(s) 1637
CaiI CAGNNNCTG 1 cut(s) 139
CciI TCATGA 1 cut(s) 1963
CfoI GCGC 2 cut(s) 1737, 2098
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 2 cut(s) 853, 2248
Cfr9I CCCGGG 1 cut(s) 299
ClaI ATCGAT 1 cut(s) 677
Csp6I GTAC 2 cut(s) 919, 2181
CviQI GTAC 2 cut(s) 919, 2181
DdeI CTNAG 8 cut(s) 126, 552, 667, 909, 983, 1713, 1796, 2229
Eam1104I CTCTTC 2 cut(s) 55, 2066
EarI CTCTTC 2 cut(s) 55, 2066
EciI GGCGGA 1 cut(s) 135
Ecl136II GAGCTC 1 cut(s) 563
Eco130I CCWWGG 2 cut(s) 25, 1986
Eco147I AGGCCT 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 565
Eco32I GATATC 2 cut(s) 903, 1128
Eco47I GGWCC 2 cut(s) 853, 2248
Eco53kI GAGCTC 1 cut(s) 563
Eco57I CTGAAG 3 cut(s) 36, 608, 1362
Eco81I CCTNAGG 1 cut(s) 909
Eco88I CYCGRG 2 cut(s) 299, 1527
EcoICRI GAGCTC 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 1551
EcoRII CCWGG 3 cut(s) 386, 1584, 2233
EcoRV GATATC 2 cut(s) 903, 1128
EcoT14I CCWWGG 2 cut(s) 25, 1986
EcoT38I GRGCYC 1 cut(s) 565
ErhI CCWWGG 2 cut(s) 25, 1986
FaqI GGGAC 3 cut(s) 738, 1450, 1547
FauI CCCGC 3 cut(s) 167, 2185, 2259
FauNDI CATATG 2 cut(s) 1195, 1507
FbaI TGATCA 1 cut(s) 1288
FblI GTMKAC 2 cut(s) 988, 2323
Fnu4HI GCNGC 7 cut(s) 249, 453, 587, 1206, 1464, 2091, 2094
FokI GGATG 7 cut(s) 307, 1357, 1442, 1472, 1602, 1916, 2003
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 7 cut(s) 249, 453, 587, 1206, 1464, 2091, 2094
FspBI CTAG 4 cut(s) 207, 393, 1878, 2120
FspI TGCGCA 1 cut(s) 2097
GlaI GCGC 2 cut(s) 1736, 2097
GluI GCNGC 7 cut(s) 249, 453, 587, 1206, 1464, 2091, 2094
GsaI CCCAGC 2 cut(s) 277, 499
GsuI CTGGAG 4 cut(s) 243, 1439, 1760, 2206
HaeII RGCGCY 1 cut(s) 1738
HaeIII GGCC 2 cut(s) 228, 1482
HapII CCGG 3 cut(s) 149, 239, 300
HhaI GCGC 2 cut(s) 1737, 2098
Hin6I GCGC 2 cut(s) 1735, 2096
HinP1I GCGC 2 cut(s) 1735, 2096
HincII GTYRAC 4 cut(s) 989, 1314, 2310, 2324
HindII GTYRAC 4 cut(s) 989, 1314, 2310, 2324
HindIII AAGCTT 1 cut(s) 1187
HpaII CCGG 3 cut(s) 149, 239, 300
HphI GGTGA 5 cut(s) 225, 292, 897, 971, 2249
Hpy99I CGWCG 1 cut(s) 2328
HpyCH4III ACNGT 7 cut(s) 104, 348, 742, 1265, 1708, 1918, 1956
HpyCH4IV ACGT 2 cut(s) 108, 2201
HpyCH4V TGCA 8 cut(s) 251, 829, 1112, 1208, 1364, 1463, 1696, 2153
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 136, 145, 248, 835, 1858
HpyF3I CTNAG 8 cut(s) 126, 552, 667, 909, 983, 1713, 1796, 2229
HpySE526I ACGT 2 cut(s) 108, 2201
HspAI GCGC 2 cut(s) 1735, 2096
KpnI GGTACC 1 cut(s) 2184
Ksp22I TGATCA 1 cut(s) 1288
LguI GCTCTTC 1 cut(s) 2066
LmnI GCTCC 6 cut(s) 61, 215, 568, 1589, 1653, 1885
Lsp1109I GCAGC 7 cut(s) 235, 439, 598, 1192, 1475, 2077, 2080
LweI GCATC 7 cut(s) 285, 869, 1027, 1355, 1450, 1807, 2140
MaeI CTAG 4 cut(s) 207, 393, 1878, 2120
MaeII ACGT 2 cut(s) 108, 2201
MaeIII GTNAC 8 cut(s) 104, 305, 350, 696, 1334, 1708, 2071, 2197
MfeI CAATTG 1 cut(s) 1697
MflI RGATCY 8 cut(s) 29, 151, 189, 266, 446, 682, 1105, 1970
MhlI GDGCHC 3 cut(s) 565, 1251, 1528
MlyI GAGTC 9 cut(s) 191, 992, 1169, 1538, 1705, 1759, 2169, 2302, 2307
MmeI TCCRAC 4 cut(s) 219, 358, 1024, 1407
MroXI GAANNNNTTC 2 cut(s) 2060, 2131
MseI TTAA 1 cut(s) 1763
MslI CAYNNNNRTG 6 cut(s) 357, 719, 1198, 1296, 1488, 1647
MspA1I CMGCKG 4 cut(s) 248, 671, 1466, 2315
MspI CCGG 3 cut(s) 149, 239, 300
MspR9I CCNGG 5 cut(s) 300, 301, 388, 1586, 2235
MunI CAATTG 1 cut(s) 1697
Mva1269I GAATGC 1 cut(s) 2194
MvaI CCWGG 3 cut(s) 388, 1586, 2235
MwoI GCNNNNNNNGC 6 cut(s) 53, 136, 145, 248, 835, 1858
NciI CCSGG 2 cut(s) 300, 301
NcoI CCATGG 1 cut(s) 1986
NdeI CATATG 2 cut(s) 1195, 1507
NlaIV GGNNCC 9 cut(s) 153, 243, 412, 684, 1655, 1972, 2128, 2182, 2250
NmuCI GTSAC 3 cut(s) 104, 350, 1708
NsbI TGCGCA 1 cut(s) 2097
NspI RCATGY 1 cut(s) 1364
NspV TTCGAA 1 cut(s) 1079
OliI CACNNNNGTG 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 1527
PagI TCATGA 1 cut(s) 1963
PaqCI CACCTGC 1 cut(s) 268
PceI AGGCCT 1 cut(s) 228
PciSI GCTCTTC 1 cut(s) 2066
PcsI WCGNNNNNNNCGW 1 cut(s) 553
PctI GAATGC 1 cut(s) 2194
PdmI GAANNNNTTC 2 cut(s) 2060, 2131
PfeI GAWTC 8 cut(s) 257, 653, 674, 1469, 1568, 1910, 2102, 2267
PkrI GCNGC 7 cut(s) 250, 454, 588, 1207, 1465, 2092, 2095
PleI GAGTC 9 cut(s) 191, 992, 1168, 1538, 1705, 1758, 2168, 2301, 2306
PpsI GAGTC 9 cut(s) 191, 992, 1168, 1538, 1705, 1758, 2168, 2301, 2306
Psp124BI GAGCTC 1 cut(s) 565
Psp6I CCWGG 3 cut(s) 386, 1584, 2233
PspFI CCCAGC 2 cut(s) 273, 495
PspGI CCWGG 3 cut(s) 386, 1584, 2233
PspN4I GGNNCC 9 cut(s) 153, 243, 412, 684, 1655, 1972, 2128, 2182, 2250
PspPI GGNCC 2 cut(s) 853, 2248
PspXI VCTCGAGB 1 cut(s) 1527
PstI CTGCAG 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 139
PsuI RGATCY 8 cut(s) 29, 151, 189, 266, 446, 682, 1105, 1970
PvuII CAGCTG 4 cut(s) 248, 671, 1466, 2315
RsaI GTAC 2 cut(s) 920, 2182
RsaNI GTAC 2 cut(s) 919, 2181
RseI CAYNNNNRTG 6 cut(s) 357, 719, 1198, 1296, 1488, 1647
SacI GAGCTC 1 cut(s) 565
SalI GTCGAC 2 cut(s) 987, 2322
SapI GCTCTTC 1 cut(s) 2066
SaqAI TTAA 1 cut(s) 1763
SatI GCNGC 7 cut(s) 249, 453, 587, 1206, 1464, 2091, 2094
Sau96I GGNCC 2 cut(s) 853, 2248
SchI GAGTC 9 cut(s) 191, 992, 1169, 1538, 1705, 1759, 2169, 2302, 2307
ScrFI CCNGG 5 cut(s) 300, 301, 388, 1586, 2235
SduI GDGCHC 3 cut(s) 565, 1251, 1528
SfaNI GCATC 7 cut(s) 285, 869, 1027, 1355, 1450, 1807, 2140
SfcI CTRYAG 2 cut(s) 249, 2016
Sfr274I CTCGAG 1 cut(s) 1527
SfuI TTCGAA 1 cut(s) 1079
SinI GGWCC 2 cut(s) 853, 2248
SlaI CTCGAG 1 cut(s) 1527
SmaI CCCGGG 1 cut(s) 301
SmiMI CAYNNNNRTG 6 cut(s) 357, 719, 1198, 1296, 1488, 1647
SmlI CTYRAG 3 cut(s) 1527, 1747, 2065
SmoI CTYRAG 3 cut(s) 1527, 1747, 2065
SseBI AGGCCT 1 cut(s) 228
SsiI CCGC 9 cut(s) 91, 97, 146, 160, 216, 782, 1741, 2192, 2252
SspI AATATT 1 cut(s) 1238
SspMI CTAG 4 cut(s) 207, 393, 1878, 2120
SstI GAGCTC 1 cut(s) 565
StuI AGGCCT 1 cut(s) 228
StyD4I CCNGG 5 cut(s) 298, 299, 386, 1584, 2233
StyI CCWWGG 2 cut(s) 25, 1986
TaaI ACNGT 7 cut(s) 104, 348, 742, 1265, 1708, 1918, 1956
TaiI ACGT 2 cut(s) 111, 2204
TaqI TCGA 7 cut(s) 677, 988, 1079, 1528, 1998, 2296, 2323
TatI WGTACW 1 cut(s) 918
TfiI GAWTC 8 cut(s) 257, 653, 674, 1469, 1568, 1910, 2102, 2267
Tru1I TTAA 1 cut(s) 1763
Tru9I TTAA 1 cut(s) 1763
TscAI CASTG 4 cut(s) 353, 1256, 1345, 1713
TseFI GTSAC 3 cut(s) 104, 350, 1708
TseI GCWGC 7 cut(s) 248, 452, 586, 1205, 1463, 2090, 2093
Tsp45I GTSAC 3 cut(s) 104, 350, 1708
TspMI CCCGGG 1 cut(s) 299
TspRI CASTG 4 cut(s) 353, 1256, 1345, 1713
VpaK11BI GGWCC 2 cut(s) 853, 2248
XapI RAATTY 6 cut(s) 165, 523, 638, 1321, 1551, 1689
XbaI TCTAGA 1 cut(s) 392
XceI RCATGY 1 cut(s) 1364
XcmI CCANNNNNNNNNTGG 3 cut(s) 503, 739, 1592
XhoI CTCGAG 1 cut(s) 1527
XmaI CCCGGG 1 cut(s) 299
XmiI GTMKAC 2 cut(s) 988, 2323
XmnI GAANNNNTTC 2 cut(s) 2060, 2131
XspI CTAG 4 cut(s) 207, 393, 1878, 2120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.