pycom11g04510

transcription repressor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0015454
Physical Location & Seq
Forward (+)
3495491 .. 3496048
558 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g04510.1

Sequence Viewer

Length: 558 bp
ATGAAGTTGAAAGCATCAGTTGCCTTGAGATCCAAGCTACTCAAACCATGCAGAAATTTCCTACAAATCTTCAGATTCAAGCTCAAAAAACCATTCTTTTTAAGAGCTCTTCGTCTCCGTCGATCTCCTTACACCCAAGTTAAAGAGGATAAAAAGTCTCTGAAGAAGAAGAGTCGGATAACTTTGTTCCTATCATCTTTCTGTTCAACCAGGAAACCGAAAGGAATGGACAGACTAGCAGCGCTCAAGAGCTTCTCTGAAAGAGGGGGTGACAGAAATGGAATACTGCACTATCCGTCACCTGTCACACCGGCGGCTATGACCGGAGGAAGGGCGGGGGCTTCTGGTAATGACGAGGTGCAAGATGCGTGCAGGAGTTTTGAGAACTATTTGGTGGAGATGATCATTGAGGAAGGGAAAGTGGGGGACTTGATGGATGTGGAAGAGCTTCTGTATTGCTGGAAGAACCTCAAGTGCCCTGTTTTCATTGATTTGGTTGGGAGATTCTATGGGGAGCTTTGCAAGGACTTGTTTTCTCCTACCAGTGATGAAAGTTAG

Protein Analysis

186

Amino Acids

21.07

Weight (kDa)

9.67

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30395
fragaria_vesca FvH4_3g40300
malus_domestica MD03G1053700.v1.1 MD11G1055300.v1.1
prunus_persica Prupe.6G042800_v2.0.a1
pyrus_communis pycom11g04510
rosa_chinensis RchiOBHm_Chr5g0072641
rosa_laevigata RLG00000036319
rosa_multiflora Rmu_sc0002088.1_g000003
rosa_roxburghii Rroxscaffold_1G00008510
rosa_rugosa Rorug05G0419000
rosa_samantha Rh5AG475900 Rh5BG496000 Rh5CG519800 Rh5DG508500
rosa_wichuraiana Rw5G044190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 314, 335
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 1 cut(s) 55
AcuI CTGAAG 2 cut(s) 55, 182
AfeI AGCGCT 1 cut(s) 243
AfiI CCNNNNNNNGG 1 cut(s) 330
AgsI TTSAA 3 cut(s) 10, 79, 207
AjnI CCWGG 1 cut(s) 209
AluBI AGCT 6 cut(s) 37, 82, 107, 252, 448, 517
AluI AGCT 6 cut(s) 37, 82, 107, 252, 448, 517
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 2 cut(s) 119, 162
AlwI GGATC 1 cut(s) 24
Aor51HI AGCGCT 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 239
ApoI RAATTY 1 cut(s) 55
Asp700I GAANNNNTTC 1 cut(s) 447
AspLEI GCGC 1 cut(s) 244
AsuHPI GGTGA 2 cut(s) 281, 291
BaeGI GKGCMC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 1 cut(s) 251
BccI CCATC 1 cut(s) 427
BciT130I CCWGG 1 cut(s) 211
BclI TGATCA 1 cut(s) 402
BcoDI GTCTC 2 cut(s) 119, 162
BfaI CTAG 1 cut(s) 236
BfoI RGCGCY 1 cut(s) 245
BisI GCNGC 2 cut(s) 240, 315
BlsI GCNGC 2 cut(s) 241, 316
Bme1390I CCNGG 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 211
BmsI GCATC 2 cut(s) 23, 355
BpuEI CTTGAG 3 cut(s) 46, 230, 455
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 1 cut(s) 330
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 1 cut(s) 543
BseBI CCWGG 1 cut(s) 211
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 1 cut(s) 330
BseNI ACTGG 1 cut(s) 543
BseSI GKGCMC 1 cut(s) 479
BseXI GCAGC 1 cut(s) 251
BsgI GTGCAG 2 cut(s) 272, 391
BsiHKAI GWGCWC 1 cut(s) 109
BsiSI CCGG 2 cut(s) 311, 324
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 1 cut(s) 330
BsmAI GTCTC 2 cut(s) 119, 162
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 109, 479
Bsp143I GATC 3 cut(s) 29, 122, 402
BspACI CCGC 2 cut(s) 314, 335
BspPI GGATC 1 cut(s) 24
BspQI GCTCTTC 2 cut(s) 114, 438
BsrFI RCCGGY 1 cut(s) 310
BsrI ACTGG 1 cut(s) 543
BssAI RCCGGY 1 cut(s) 310
BssMI GATC 3 cut(s) 29, 122, 402
Bst2UI CCWGG 1 cut(s) 211
Bst6I CTCTTC 3 cut(s) 114, 164, 438
BstAPI GCANNNNNTGC 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 370
BstF5I GGATG 1 cut(s) 442
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 3 cut(s) 32, 125, 405
BstMAI GTCTC 2 cut(s) 119, 162
BstMBI GATC 3 cut(s) 29, 122, 402
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 209
BstSLI GKGCMC 1 cut(s) 479
BstV1I GCAGC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BtsCI GGATG 1 cut(s) 442
BtsIMutI CAGTG 1 cut(s) 550
Cac8I GCNNGC 1 cut(s) 370
CfoI GCGC 1 cut(s) 244
Cfr10I RCCGGY 1 cut(s) 310
CviAII CATG 1 cut(s) 48
CviJI RGCY 8 cut(s) 37, 82, 107, 252, 317, 341, 448, 517
CviKI_1 RGCY 8 cut(s) 37, 82, 107, 252, 317, 341, 448, 517
DpnI GATC 3 cut(s) 31, 124, 404
DpnII GATC 3 cut(s) 29, 122, 402
Eam1104I CTCTTC 3 cut(s) 114, 164, 438
EarI CTCTTC 3 cut(s) 114, 164, 438
Ecl136II GAGCTC 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 109
Eco47III AGCGCT 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 107
Eco57I CTGAAG 2 cut(s) 55, 182
EcoICRI GAGCTC 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 209
EcoT38I GRGCYC 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 1 cut(s) 51
FaiI YATR 3 cut(s) 49, 320, 510
FaqI GGGAC 1 cut(s) 440
FatI CATG 1 cut(s) 47
FauI CCCGC 1 cut(s) 328
FbaI TGATCA 1 cut(s) 402
Fnu4HI GCNGC 2 cut(s) 240, 315
FokI GGATG 1 cut(s) 449
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 2 cut(s) 240, 315
FspBI CTAG 1 cut(s) 236
GlaI GCGC 1 cut(s) 243
GluI GCNGC 2 cut(s) 240, 315
HaeII RGCGCY 1 cut(s) 245
HapII CCGG 2 cut(s) 311, 324
HhaI GCGC 1 cut(s) 244
Hin1II CATG 1 cut(s) 51
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HinfI GANTC 3 cut(s) 75, 172, 504
HpaII CCGG 2 cut(s) 311, 324
HphI GGTGA 2 cut(s) 281, 291
Hpy188I TCNGA 4 cut(s) 74, 162, 177, 259
Hpy188III TCNNGA 1 cut(s) 247
Hpy99I CGWCG 1 cut(s) 123
HpyAV CCTTC 2 cut(s) 324, 407
HpyCH4V TGCA 5 cut(s) 51, 289, 361, 372, 522
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
Hsp92II CATG 1 cut(s) 51
HspAI GCGC 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 402
Kzo9I GATC 3 cut(s) 29, 122, 402
LguI GCTCTTC 2 cut(s) 114, 438
LmnI GCTCC 1 cut(s) 514
LpnPI CCDG 9 cut(s) 196, 223, 315, 324, 330, 337, 358, 445, 492
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 2 cut(s) 23, 355
MaeI CTAG 1 cut(s) 236
MaeIII GTNAC 3 cut(s) 269, 297, 304
MalI GATC 3 cut(s) 31, 124, 404
MboI GATC 3 cut(s) 29, 122, 402
MboII GAAGA 7 cut(s) 61, 101, 175, 178, 181, 455, 475
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 2 cut(s) 109, 479
MluCI AATT 1 cut(s) 55
MlyI GAGTC 1 cut(s) 181
MmeI TCCRAC 1 cut(s) 155
MnlI CCTC 6 cut(s) 139, 257, 320, 349, 403, 479
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 2 cut(s) 101, 141
MspI CCGG 2 cut(s) 311, 324
MspR9I CCNGG 1 cut(s) 211
MvaI CCWGG 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 20
NdeII GATC 3 cut(s) 29, 122, 402
NlaIII CATG 1 cut(s) 51
NmuCI GTSAC 3 cut(s) 269, 297, 304
PciSI GCTCTTC 2 cut(s) 114, 438
PcsI WCGNNNNNNNCGW 1 cut(s) 118
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 2 cut(s) 75, 504
PkrI GCNGC 2 cut(s) 241, 316
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 209
PspGI CCWGG 1 cut(s) 209
PsuI RGATCY 1 cut(s) 29
SacI GAGCTC 1 cut(s) 109
SapI GCTCTTC 2 cut(s) 114, 438
SaqAI TTAA 2 cut(s) 101, 141
SatI GCNGC 2 cut(s) 240, 315
Sau3AI GATC 3 cut(s) 29, 122, 402
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 211
SduI GDGCHC 2 cut(s) 109, 479
SetI ASST 9 cut(s) 39, 84, 109, 254, 304, 360, 450, 471, 519
SfaNI GCATC 2 cut(s) 23, 355
SgrAI CRCCGGYG 1 cut(s) 310
SmlI CTYRAG 3 cut(s) 25, 245, 470
SmoI CTYRAG 3 cut(s) 25, 245, 470
Sse9I AATT 1 cut(s) 55
SsiI CCGC 2 cut(s) 314, 335
SspMI CTAG 1 cut(s) 236
SstI GAGCTC 1 cut(s) 109
StyD4I CCNGG 1 cut(s) 209
TaqI TCGA 1 cut(s) 121
TasI AATT 1 cut(s) 55
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 2 cut(s) 75, 504
Tru1I TTAA 2 cut(s) 101, 141
Tru9I TTAA 2 cut(s) 101, 141
TscAI CASTG 1 cut(s) 550
TseFI GTSAC 3 cut(s) 269, 297, 304
TseI GCWGC 1 cut(s) 239
Tsp45I GTSAC 3 cut(s) 269, 297, 304
TspDTI ATGAA 2 cut(s) 17, 475
TspGWI ACGGA 2 cut(s) 107, 285
TspRI CASTG 1 cut(s) 550
XapI RAATTY 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.