pycom11g08860

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0014119
Physical Location & Seq
Forward (+)
6993801 .. 6996596
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g08860.2

Sequence Viewer

Length: 1335 bp
ATGAAGTGCTTCCATTTCACTAATGGGGAGGGCCGGGACAACGAGGAACCCGGCGTCGTTCCCCGGGCGACGTCGAAGGTGTCGTGGGTGCGCTCCCTCAGTGTGGCCTCCAGCAGCTACGGCACCACTCGGCGGTCGGAGTTCGACTCGGACTCGAGGGACCTGTCCGACTCTGTGGCGTTCCAGGAGCTGCTGAGTCTGCGGCGGGCCAATGCTCTGAGGGTGTTCACGTTCTCGGAGCTGAAGGCGGCGAGCAGGGGGTTCAGCAGGGCGTTACTGATTGGAGAAGGAGGTTTTGGGTGCGTTTACAGAGGAATCGTCAGAGGCTGTAATGTCGGCGACGGGAAAATGGACGTTGCTATCAAACAGTTGAATCGGAATGGCTTCCAGGGGCATAAGGAATGGATTAATGAAGTAAACTTGTTGGGCGTTGTCAAACACCCAAATCTTGTTAAGTTAGTAGGATACTGTGCAGAAGATGATGAAAGGGGGATTCAACGGCTATTGGTTTATGAACTTATGCGTAATAAAAGCTTGGAGGACCATCTATGGGATCGATCTTCATCATCTCTCCCATGGATGACAAGACTAAGAATTGCTCAAGATGCAGCCCGCGGATTGGCATATCTACATGAAGAGATGGATTTTCAGCTAATATTCCGCGACTTTAAACCATCAAATATTCTGCTAGATGAGGACTTCATTGCAAAGCTTTCAGACTTTGGACTAGCGAGGCAGGGACCTCCAGAGGGGACAAGTCACGTTTCAACAACAGTTGTGGGAACAGTAGGTTATGCGGCTCCTGAATATGTACATACGGGGAAGCTAACAGCTAAAAGTGATGTGTGGAGCTTCGGAGTGGTTCTGTATGAGCTCATCACAGGAAGGAGGGCGGTGGAGAGAAATTTGCCCCGCAGTGAGCAAAAGCTTTTGGAATGGGTCAAGCCCTATGTTCCAGACTCAAAGAAATTCCACCTTATTTTGGACCCTCGGCTTGAAGGACAGTATTGTATCAAATCAGCTCAAAACCTTGCATCATTAGCTAACCGGTGTCTATCAAAACAACCAAAGTCTCGCCCAAAAATGAGTGAGGTGGTTGGGGTTTTGGGATGCATCATAGATGAGCTGTCACCTCAAGACGAAGTAGTCCCTGAACCTGTGATTGAAACAGAAGAATCAAATGAAGAAACCGTGGAGGAGACTGAGATCGAGTCTACCAAACAAGGAAACAAAAAAAGGGTTTTCGACATAAAAGACATGGTAAACTTTAGAAACAAATCTATTGGGAAGTTGGATTGGAGAAACTGGACCCCGGGGTTGATAAGAACTTGGTGA

Protein Analysis

445

Amino Acids

50.13

Weight (kDa)

7.09

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014119)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28940 AT2G28940
fragaria_vesca FvH4_3g36210 FvH4_3g36210
malus_domestica MD00G1012400.v1.1 MD03G1092100.v1.1
prunus_persica Prupe.6G076100_v2.0.a1
pyrus_communis pycom03g07380 pycom11g08860
rosa_chinensis RchiOBHm_Chr5g0064981
rosa_laevigata RLG00000035746
rosa_multiflora Rmu_sc0003352.1_g000019
rosa_roxburghii Rroxscaffold_1G00016330
rosa_rugosa Rorug05G0367100
rosa_samantha Rh5BG440400 Rh5CG463000
rosa_wichuraiana Rw5G039980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1145
AatII GACGTC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 1214
AccII CGCG 2 cut(s) 615, 663
AclWI GGATC 1 cut(s) 561
AcsI RAATTY 2 cut(s) 904, 968
AcuI CTGAAG 1 cut(s) 263
AcyI GRCGYC 2 cut(s) 54, 71
AfaI GTAC 1 cut(s) 813
AfiI CCNNNNNNNGG 6 cut(s) 103, 132, 550, 619, 749, 982
AgeI ACCGGT 1 cut(s) 1047
AgsI TTSAA 5 cut(s) 373, 497, 768, 998, 1166
AjnI CCWGG 2 cut(s) 183, 387
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
Alw21I GWGCWC 1 cut(s) 876
Alw26I GTCTC 2 cut(s) 1077, 1193
AlwI GGATC 1 cut(s) 561
AlwNI CAGNNNCTG 2 cut(s) 190, 327
Ama87I CYCGRG 3 cut(s) 63, 154, 1312
AoxI GGCC 3 cut(s) 31, 105, 207
ApeKI GCWGC 3 cut(s) 114, 190, 608
ApoI RAATTY 2 cut(s) 904, 968
AseI ATTAAT 1 cut(s) 408
AsiGI ACCGGT 1 cut(s) 1047
Asp700I GAANNNNTTC 2 cut(s) 8, 383
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 7 cut(s) 31, 160, 207, 541, 740, 985, 1308
AsuC2I CCSGG 6 cut(s) 35, 51, 64, 65, 1313, 1314
AsuHPI GGTGA 1 cut(s) 1122
AvaI CYCGRG 3 cut(s) 63, 154, 1312
AvaII GGWCC 5 cut(s) 160, 541, 740, 985, 1308
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 876
Bbv12I GWGCWC 1 cut(s) 876
BbvI GCAGC 3 cut(s) 126, 177, 620
BccI CCATC 3 cut(s) 552, 634, 682
BceAI ACGGC 2 cut(s) 136, 515
BciT130I CCWGG 2 cut(s) 185, 389
BciVI GTATCC 1 cut(s) 458
BcnI CCSGG 6 cut(s) 35, 51, 64, 65, 1313, 1314
BcoDI GTCTC 2 cut(s) 1077, 1193
BfaI CTAG 2 cut(s) 689, 728
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 6 cut(s) 115, 191, 203, 249, 609, 798
BlsI GCNGC 6 cut(s) 116, 192, 204, 250, 610, 799
Bme1390I CCNGG 8 cut(s) 35, 51, 64, 65, 185, 389, 1313, 1314
Bme18I GGWCC 5 cut(s) 160, 541, 740, 985, 1308
BmeT110I CYCGRG 3 cut(s) 63, 154, 1312
BmgT120I GGNCC 7 cut(s) 31, 160, 207, 541, 740, 985, 1308
BmiI GGNNCC 7 cut(s) 48, 124, 161, 741, 801, 987, 1310
BmrFI CCNGG 8 cut(s) 35, 51, 64, 65, 185, 389, 1313, 1314
BmsI GCATC 4 cut(s) 595, 1043, 1100, 1122
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 2 cut(s) 94, 729
BpuEI CTTGAG 2 cut(s) 585, 1119
BpuMI CCSGG 6 cut(s) 35, 51, 64, 65, 1313, 1314
Bsa29I ATCGAT 1 cut(s) 556
BsaBI GATNNNNATC 1 cut(s) 562
BsaHI GRCGYC 2 cut(s) 54, 71
BsaWI WCCGGW 1 cut(s) 1047
Bsc4I CCNNNNNNNGG 6 cut(s) 103, 132, 550, 619, 749, 982
Bse118I RCCGGY 1 cut(s) 1047
Bse1I ACTGG 1 cut(s) 1310
Bse3DI GCAATG 1 cut(s) 702
Bse8I GATNNNNATC 1 cut(s) 562
BseBI CCWGG 2 cut(s) 185, 389
BseCI ATCGAT 1 cut(s) 556
BseGI GGATG 2 cut(s) 585, 1115
BseJI GATNNNNATC 1 cut(s) 562
BseLI CCNNNNNNNGG 6 cut(s) 103, 132, 550, 619, 749, 982
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 4 cut(s) 112, 185, 209, 1194
BseNI ACTGG 1 cut(s) 1310
BseRI GAGGAG 1 cut(s) 1211
BseXI GCAGC 3 cut(s) 126, 177, 620
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 2 cut(s) 615, 663
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 3 cut(s) 33, 107, 209
BshNI GGYRCC 1 cut(s) 122
BshTI ACCGGT 1 cut(s) 1047
BshVI ATCGAT 1 cut(s) 556
BsiEI CGRYCG 1 cut(s) 137
BsiHKAI GWGCWC 1 cut(s) 876
BsiHKCI CYCGRG 3 cut(s) 63, 154, 1312
BsiSI CCGG 5 cut(s) 34, 51, 64, 1048, 1313
BslFI GGGAC 5 cut(s) 50, 173, 753, 766, 1133
BslI CCNNNNNNNGG 6 cut(s) 103, 132, 550, 619, 749, 982
BsmAI GTCTC 2 cut(s) 1077, 1193
BsmFI GGGAC 5 cut(s) 50, 173, 753, 766, 1133
BsnI GGCC 3 cut(s) 33, 107, 209
BsoBI CYCGRG 3 cut(s) 63, 154, 1312
Bsp1286I GDGCHC 1 cut(s) 876
Bsp1407I TGTACA 1 cut(s) 811
Bsp143I GATC 3 cut(s) 553, 557, 1206
Bsp19I CCATGG 1 cut(s) 575
BspANI GGCC 3 cut(s) 33, 107, 209
BspCNI CTCAG 4 cut(s) 111, 186, 210, 1195
BspDI ATCGAT 1 cut(s) 556
BspFNI CGCG 2 cut(s) 615, 663
BspLI GGNNCC 7 cut(s) 48, 124, 161, 741, 801, 987, 1310
BspPI GGATC 1 cut(s) 561
BspT107I GGYRCC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 702
BsrFI RCCGGY 1 cut(s) 1047
BsrGI TGTACA 1 cut(s) 811
BsrI ACTGG 1 cut(s) 1310
BssAI RCCGGY 1 cut(s) 1047
BssMI GATC 3 cut(s) 553, 557, 1206
BssNI GRCGYC 2 cut(s) 54, 71
BssT1I CCWWGG 1 cut(s) 575
Bst2UI CCWGG 2 cut(s) 185, 389
Bst4CI ACNGT 6 cut(s) 369, 470, 775, 787, 1005, 1192
Bst6I CTCTTC 1 cut(s) 630
BstACI GRCGYC 2 cut(s) 54, 71
BstAUI TGTACA 1 cut(s) 811
BstC8I GCNNGC 3 cut(s) 207, 253, 613
BstDEI CTNAG 5 cut(s) 98, 194, 218, 590, 1203
BstDSI CCRYGG 3 cut(s) 575, 613, 1191
BstF5I GGATG 2 cut(s) 585, 1115
BstFNI CGCG 2 cut(s) 615, 663
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 3 cut(s) 556, 560, 1209
BstMAI GTCTC 2 cut(s) 1077, 1193
BstMBI GATC 3 cut(s) 553, 557, 1206
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 4 cut(s) 120, 199, 605, 1040
BstNI CCWGG 2 cut(s) 185, 389
BstSCI CCNGG 8 cut(s) 33, 49, 62, 63, 183, 387, 1311, 1312
BstUI CGCG 2 cut(s) 615, 663
BstV1I GCAGC 3 cut(s) 126, 177, 620
Bsu15I ATCGAT 1 cut(s) 556
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 3 cut(s) 33, 107, 209
BsuTUI ATCGAT 1 cut(s) 556
BtgI CCRYGG 3 cut(s) 575, 613, 1191
BtsCI GGATG 2 cut(s) 585, 1115
BtsI GCAGTG 1 cut(s) 922
BtsIMutI CAGTG 2 cut(s) 106, 922
Cac8I GCNNGC 3 cut(s) 207, 253, 613
CaiI CAGNNNCTG 2 cut(s) 190, 327
CfoI GCGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 1047
Cfr13I GGNCC 7 cut(s) 31, 160, 207, 541, 740, 985, 1308
Cfr42I CCGCGG 1 cut(s) 616
Cfr9I CCCGGG 2 cut(s) 63, 1312
ClaI ATCGAT 1 cut(s) 556
CseI GACGC 1 cut(s) 43
Csp6I GTAC 1 cut(s) 812
CspAI ACCGGT 1 cut(s) 1047
CspCI CAANNNNNGTGG 2 cut(s) 759, 794
CviAII CATG 3 cut(s) 576, 632, 1258
CviQI GTAC 1 cut(s) 812
DdeI CTNAG 5 cut(s) 98, 194, 218, 590, 1203
DpnI GATC 3 cut(s) 555, 559, 1208
DpnII GATC 3 cut(s) 553, 557, 1206
DraI TTTAAA 1 cut(s) 670
DrdI GACNNNNNNGTC 1 cut(s) 1145
DseDI GACNNNNNNGTC 1 cut(s) 1145
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
Ecl136II GAGCTC 1 cut(s) 874
Eco130I CCWWGG 1 cut(s) 575
Eco24I GRGCYC 1 cut(s) 876
Eco47I GGWCC 5 cut(s) 160, 541, 740, 985, 1308
Eco53kI GAGCTC 1 cut(s) 874
Eco57I CTGAAG 1 cut(s) 263
Eco88I CYCGRG 3 cut(s) 63, 154, 1312
EcoICRI GAGCTC 1 cut(s) 874
EcoO109I RGGNCCY 2 cut(s) 160, 740
EcoRII CCWGG 2 cut(s) 183, 387
EcoT14I CCWWGG 1 cut(s) 575
EcoT22I ATGCAT 1 cut(s) 1115
EcoT38I GRGCYC 1 cut(s) 876
ErhI CCWWGG 1 cut(s) 575
FaeI CATG 3 cut(s) 579, 635, 1261
FaqI GGGAC 5 cut(s) 50, 173, 753, 766, 1133
FatI CATG 3 cut(s) 575, 631, 1257
FauI CCCGC 3 cut(s) 198, 620, 920
FblI GTMKAC 1 cut(s) 1214
Fnu4HI GCNGC 6 cut(s) 115, 191, 203, 249, 609, 798
FokI GGATG 2 cut(s) 592, 1122
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 6 cut(s) 115, 191, 203, 249, 609, 798
FspBI CTAG 2 cut(s) 689, 728
GlaI GCGC 1 cut(s) 92
GluI GCNGC 6 cut(s) 115, 191, 203, 249, 609, 798
GsuI CTGGAG 2 cut(s) 94, 729
HaeIII GGCC 3 cut(s) 33, 107, 209
HapII CCGG 5 cut(s) 34, 51, 64, 1048, 1313
HgaI GACGC 1 cut(s) 43
HhaI GCGC 1 cut(s) 93
Hin1I GRCGYC 2 cut(s) 54, 71
Hin1II CATG 3 cut(s) 579, 635, 1261
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HindIII AAGCTT 3 cut(s) 532, 710, 926
HpaII CCGG 5 cut(s) 34, 51, 64, 1048, 1313
HphI GGTGA 1 cut(s) 1122
Hpy166II GTNNAC 5 cut(s) 228, 307, 418, 1215, 1264
Hpy188I TCNGA 9 cut(s) 139, 151, 169, 219, 238, 323, 378, 718, 857
Hpy188III TCNNGA 5 cut(s) 602, 746, 803, 956, 1136
Hpy8I GTNNAC 5 cut(s) 228, 307, 418, 1215, 1264
Hpy99I CGWCG 4 cut(s) 59, 73, 76, 344
HpyAV CCTTC 5 cut(s) 70, 238, 281, 879, 992
HpyCH4III ACNGT 6 cut(s) 369, 470, 775, 787, 1005, 1192
HpyCH4IV ACGT 4 cut(s) 71, 230, 354, 762
HpyCH4V TGCA 5 cut(s) 473, 608, 707, 1034, 1113
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 199, 605, 1040
HpyF3I CTNAG 5 cut(s) 98, 194, 218, 590, 1203
HpySE526I ACGT 4 cut(s) 71, 230, 354, 762
Hsp92I GRCGYC 2 cut(s) 54, 71
Hsp92II CATG 3 cut(s) 579, 635, 1261
HspAI GCGC 1 cut(s) 91
KspI CCGCGG 1 cut(s) 616
Kzo9I GATC 3 cut(s) 553, 557, 1206
LmnI GCTCC 5 cut(s) 98, 187, 238, 805, 849
Lsp1109I GCAGC 3 cut(s) 126, 177, 620
LweI GCATC 4 cut(s) 595, 1043, 1100, 1122
MaeI CTAG 2 cut(s) 689, 728
MaeII ACGT 4 cut(s) 71, 230, 354, 762
MaeIII GTNAC 3 cut(s) 273, 758, 1128
MalI GATC 3 cut(s) 555, 559, 1208
MboI GATC 3 cut(s) 553, 557, 1206
MboII GAAGA 5 cut(s) 488, 552, 647, 1184, 1196
MhlI GDGCHC 1 cut(s) 876
MluCI AATT 3 cut(s) 594, 904, 968
MlyI GAGTC 6 cut(s) 140, 146, 164, 205, 953, 1220
MmeI TCCRAC 3 cut(s) 117, 192, 1272
Mph1103I ATGCAT 1 cut(s) 1115
MroXI GAANNNNTTC 2 cut(s) 8, 383
MseI TTAA 3 cut(s) 408, 453, 669
MspA1I CMGCKG 1 cut(s) 615
MspI CCGG 5 cut(s) 34, 51, 64, 1048, 1313
MspR9I CCNGG 8 cut(s) 35, 51, 64, 65, 185, 389, 1313, 1314
MvaI CCWGG 2 cut(s) 185, 389
MvnI CGCG 2 cut(s) 615, 663
MwoI GCNNNNNNNGC 4 cut(s) 120, 199, 605, 1040
NciI CCSGG 6 cut(s) 35, 51, 64, 65, 1313, 1314
NcoI CCATGG 1 cut(s) 575
NdeII GATC 3 cut(s) 553, 557, 1206
NlaIII CATG 3 cut(s) 579, 635, 1261
NlaIV GGNNCC 7 cut(s) 48, 124, 161, 741, 801, 987, 1310
NmeAIII GCCGAG 2 cut(s) 109, 970
NmuCI GTSAC 2 cut(s) 758, 1128
NsiI ATGCAT 1 cut(s) 1115
PaeR7I CTCGAG 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PdmI GAANNNNTTC 2 cut(s) 8, 383
PfeI GAWTC 4 cut(s) 315, 373, 493, 1175
PfoI TCCNGGA 1 cut(s) 183
PinAI ACCGGT 1 cut(s) 1047
PkrI GCNGC 6 cut(s) 116, 192, 204, 250, 610, 799
PleI GAGTC 6 cut(s) 140, 146, 164, 204, 953, 1219
PpsI GAGTC 6 cut(s) 140, 146, 164, 204, 953, 1219
PpuMI RGGWCCY 2 cut(s) 160, 740
PshBI ATTAAT 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 876
Psp5II RGGWCCY 2 cut(s) 160, 740
Psp6I CCWGG 2 cut(s) 183, 387
PspGI CCWGG 2 cut(s) 183, 387
PspN4I GGNNCC 7 cut(s) 48, 124, 161, 741, 801, 987, 1310
PspPI GGNCC 7 cut(s) 31, 160, 207, 541, 740, 985, 1308
PspPPI RGGWCCY 2 cut(s) 160, 740
PspXI VCTCGAGB 1 cut(s) 154
PstNI CAGNNNCTG 2 cut(s) 190, 327
RsaI GTAC 1 cut(s) 813
RsaNI GTAC 1 cut(s) 812
SacI GAGCTC 1 cut(s) 876
SacII CCGCGG 1 cut(s) 616
SaqAI TTAA 3 cut(s) 408, 453, 669
SatI GCNGC 6 cut(s) 115, 191, 203, 249, 609, 798
Sau3AI GATC 3 cut(s) 553, 557, 1206
Sau96I GGNCC 7 cut(s) 31, 160, 207, 541, 740, 985, 1308
SchI GAGTC 6 cut(s) 140, 146, 164, 205, 953, 1220
ScrFI CCNGG 8 cut(s) 35, 51, 64, 65, 185, 389, 1313, 1314
SduI GDGCHC 1 cut(s) 876
SfaNI GCATC 4 cut(s) 595, 1043, 1100, 1122
Sfr274I CTCGAG 1 cut(s) 154
Sfr303I CCGCGG 1 cut(s) 616
SgrBI CCGCGG 1 cut(s) 616
SinI GGWCC 5 cut(s) 160, 541, 740, 985, 1308
SlaI CTCGAG 1 cut(s) 154
SmaI CCCGGG 2 cut(s) 65, 1314
SmlI CTYRAG 3 cut(s) 154, 600, 1134
SmoI CTYRAG 3 cut(s) 154, 600, 1134
Sse9I AATT 3 cut(s) 594, 904, 968
SspI AATATT 2 cut(s) 657, 682
SspMI CTAG 2 cut(s) 689, 728
SstI GAGCTC 1 cut(s) 876
StyD4I CCNGG 8 cut(s) 33, 49, 62, 63, 183, 387, 1311, 1312
StyI CCWWGG 1 cut(s) 575
TaaI ACNGT 6 cut(s) 369, 470, 775, 787, 1005, 1192
TaiI ACGT 4 cut(s) 74, 233, 357, 765
TaqI TCGA 6 cut(s) 74, 144, 155, 556, 1209, 1245
TasI AATT 3 cut(s) 594, 904, 968
TatI WGTACW 1 cut(s) 811
TauI GCSGC 3 cut(s) 205, 251, 800
TfiI GAWTC 4 cut(s) 315, 373, 493, 1175
Tru1I TTAA 3 cut(s) 408, 453, 669
Tru9I TTAA 3 cut(s) 408, 453, 669
TscAI CASTG 2 cut(s) 106, 922
TseFI GTSAC 2 cut(s) 758, 1128
TseI GCWGC 3 cut(s) 114, 190, 608
Tsp45I GTSAC 2 cut(s) 758, 1128
TspDTI ATGAA 8 cut(s) 17, 426, 498, 528, 552, 648, 691, 1197
TspMI CCCGGG 2 cut(s) 63, 1312
TspRI CASTG 2 cut(s) 106, 922
VpaK11BI GGWCC 5 cut(s) 160, 541, 740, 985, 1308
VspI ATTAAT 1 cut(s) 408
XapI RAATTY 2 cut(s) 904, 968
XcmI CCANNNNNNNNNTGG 1 cut(s) 20
XhoI CTCGAG 1 cut(s) 154
XmaI CCCGGG 2 cut(s) 63, 1312
XmiI GTMKAC 1 cut(s) 1214
XmnI GAANNNNTTC 2 cut(s) 8, 383
XspI CTAG 2 cut(s) 689, 728
ZraI GACGTC 1 cut(s) 72
Zsp2I ATGCAT 1 cut(s) 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.