Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0013863

Physical Location & Seq

Genomic Coordinates Reverse (-)

16141405 .. 16145896

Gene Structure 4492 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2427 bp

ATGAACCAAGTGTCGTCAATTAGTTTTGGGCCTTATGGCTCTCTCTATTTCTCAGTTAAGCGTGCCGCTTTTCTTCGCTGTACCCACCACCTCCATTTCCACACTACCTCTACCAGAGCAGTGGCCTCCCACATTGTTGGTCTTCCCCGCATAGGACCGAAAAGAGAGCTCAAGTTTGCCTTGGAATCTTTCTGGGATGGGAAGAGCAGTGCCGAAGAATTGCAAAAGGCGGCTGCAGGTCTTAGGTCCAGCATTTGGAAGCAGCTGGCTGATGCTGGTATTCAGTATATTCCAAGCAACACCTTCTCGTACTATGATCAGATGTTAGACACCACAGCCATGCTTGGGGCTGTACCCCCTAGGTATGGTTGGAAACACAGCGAGATTGGCTTCGAGACCTACTTTTCCATGGCTAGGGGAAATGTAAATGTTCCTGCTATGGAAATGACCAAGTGGTTTGACACCAACTACCATTACATTGTCCCTGAATTGGGCCCACATATTAGGTTCTCTTATGCATCGCACAAGGCAGTGGATGAATTCAAGGAGGCCAAGGCTCTTGGAGTGGATACAATTCCCGTCCTTATAGGACCTGTATCCTACTTGTTGCTATCAAAACCAGCAAGGGGTATTGAACAATCTTTTCCCCCCCTCTCCCTACTTCGCTATATTCTTCCAGTTTACAAGGAGGTTATGGCTGAACTAAAGGCAGCTGGTGCTACCTGGGTCCAGTTTGATGAGCCCGCCCTTGTATTGGATCTCGATGCTAATCAATTGGAAGCATTTACTCATACGTACTCGGAACTAGAGTCATCTTTCTCTGGTTTAAATGTTCTGATTGAGACATACTTTTCTGATGTTACTGCTGAAGCGTACAAAACAATTACCTCTTTGAAGGGTGTTACTGGTTATGGGTTTGACCTGGTTCGTGGAGCAAAGACCCTTGATCTGATTAAGGGTGGATTTCCTATTGGAAAACACCTTTTTGCTGGAGTGGTTGATGGAAGGAATATATGGGCCAATGATCTTGCATCCTCCGTCAATATCCTGCAGGCTCTTGAAAGCATTGTTGGAAAAGACAAGATTGTGGTTTCAACATCCTGCTCCCTTCTCCACACTGCAGTTGATTTAGAGAACGAGACTAACTTGGATAAAGATATTAAACCATGGCTTGCATTTGCAACCCAAAAAGTTATTGAAGTAAATGCCTTGGCTAAAGCATTGTCCGGACAGAAGGATGAGGCATTTTTCACTGCCAATGCTGCAGCTCAAGCTTCAAGAAAATCCTCCCCCATAGTGACGAATGAGGCTGTGCAAAAGGCTGTTGCTGCTCTGAAGGGCTCTGACCACGGCCGACCCACAAATGTGAGTACAAGGCTGGATGCTCAGCAAAAGAAGCTGAACCTTCCAATTCTTCCAACAACAACCATTGGATCTTTCCCTCAAACAATGGATCTTAGAAGAGTGCGCCGGGAATATAAGGATAAAAAAATCTCTGAGGAAGATTATTTCAATGCAATGAAGGAAGAAATTAACAAAGTTGTCAAGCTTCAGGAAGAGCTGGACATTGATGTATTGGTGCACGGAGAGCCTGAGAGGAATGACATGGTTGAGTACTTTGGAGAGCAATTATCTGGTTTTGCGTTTACTTCCAATGGGTGGGTCCAATCTTACGGTTCTCGCTGTGTAAAGCCACCTATTATCTATGGTGATGTGAGTCGCCCAGATCCCATGACGGTTTTCTGGTCATCGCTAGCCCAGGGAATGACAAAGAGACCTATGAAGGGAATGCTCACTGGACCTGTCACCCTTATGAACTGGTCTTTTGTGAGAAATGACCAGCCCAGATTCGAGACCTGCTATCAGATTGCTTTGGCCATGAAGGATGAGGTTGAGGATCTTGAGAAAGCGGGAATTACTGTGATCCAGATTGATGAGCCTGCTCTAAGAGAAGGTTTGCCTTTGAGGAAGGCCGAGCAGTCTCTCTATCTTGATTGGGCTGTTCACTCCTTCAGGATAACTAACTGTAGTGTTCAGGATACTACTCAGATCCACACCCACATGTGCTACTCAAATTTCAATGACATAATTCACTCAATCATTGACATGGATGCGGATGTGATCACAATCGAGAACTCCAAATCGGATGAGAGACTGCTCTCGGTCCTCCCTGAGGGACTGAAGTTTGGTTCTGGCATTGGTCCTGGTGTCTATGATGTGCACTCACCAAGGATCCCGTCAGACACGGAACTCAGTGTCAAAATTGAGAATATTGTTAAGGTCCTTGAGAAGAACATCATCTGGGTTAACCCCGACTGTGGTCTCAAGACACGCAAATACTCTGAAGTCCAGCCTGCCCTCAACAACATGGTTACTGCTGCCAAGTACAATCGGAAGATGTTGGGCATTCAGGCAAGTAACGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0003871 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008652 GO:0008705 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0032259 GO:0042084 GO:0042085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050667 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00450 ko01100 ko01110 ko01230 map00270 map00450 map01100 map01110 map01230

Pfam Domains

Protein Families

Meth_synt_1 Meth_synt_2

Protein Analysis

809

Amino Acids

89.93

Weight (kDa)

6.14

Isoelectric Point (pI)

34.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Meth_synt_1	PF08267	43 - 356	4.2e-116	Cobalamin-independent synthase, N-terminal domain
Meth_synt_2	PF01717	472 - 795	5.2e-154	Cobalamin-independent synthase, Catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20980 AT5G20980
fragaria_vesca	FvH4_3g27060 FvH4_3g27060 FvH4_3g27060 FvH4_3g27060 FvH4_3g27060
malus_domestica	MD11G1179000.v1.1
prunus_persica	Prupe.6G151800_v2.0.a1
pyrus_communis	pycom11g15450
rosa_chinensis	RchiOBHm_Chr5g0049641
rosa_laevigata	RLG00000034629
rosa_multiflora	Rmu_sc0013777.1_g000007
rosa_roxburghii	Rroxscaffold_1G00032020
rosa_rugosa	Rorug05G0249400
rosa_samantha	Rh5AG325900
rosa_wichuraiana	Rw5G030800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	227, 1865
AccB7I	CCANNNNNTGG	2 cut(s)	255, 1659
AccIII	TCCGGA	1 cut(s)	1226
AciI	CCGC	6 cut(s)	66, 148, 230, 744, 1910, 2114
AclWI	GGATC	9 cut(s)	765, 1441, 1461, 1721, 1905, 1918, 2044, 2227, 2240
AcoI	YGGCCR	2 cut(s)	1351, 1875
AcsI	RAATTY	2 cut(s)	539, 2074
AcuI	CTGAAG	6 cut(s)	888, 1355, 1535, 1996, 2201, 2364
AfaI	GTAC	8 cut(s)	82, 311, 354, 797, 875, 1372, 1616, 2387
AflIII	ACRYGT	1 cut(s)	2061
AgsI	TTSAA	9 cut(s)	544, 635, 895, 1061, 1095, 1199, 1278, 1513, 2080
AjnI	CCWGG	4 cut(s)	722, 921, 1758, 2203
AjuI	GAANNNNNNNTTGG	2 cut(s)	1053, 1085
AleI	CACNNNNGTG	1 cut(s)	1364
AluBI	AGCT	8 cut(s)	169, 265, 713, 1268, 1274, 1399, 1549, 1561
AluI	AGCT	8 cut(s)	169, 265, 713, 1268, 1274, 1399, 1549, 1561
Alw21I	GWGCWC	3 cut(s)	171, 1584, 2223
Alw26I	GTCTC	8 cut(s)	389, 836, 1133, 1768, 1847, 1986, 2146, 2327
Alw44I	GTGCAC	2 cut(s)	1580, 2219
AlwI	GGATC	9 cut(s)	765, 1441, 1461, 1721, 1905, 1918, 2044, 2227, 2240
Aor13HI	TCCGGA	1 cut(s)	1226
AoxI	GGCC	8 cut(s)	29, 123, 493, 549, 1017, 1351, 1875, 1971
ApaI	GGGCCC	1 cut(s)	497
ApaLI	GTGCAC	2 cut(s)	1580, 2219
ApeKI	GCWGC	7 cut(s)	233, 262, 710, 1262, 1265, 1328, 2378
ApoI	RAATTY	2 cut(s)	539, 2074
AspA2I	CCTAGG	1 cut(s)	359
AspLEI	GCGC	1 cut(s)	1470
AsuC2I	CCSGG	1 cut(s)	1472
AsuHPI	GGTGA	3 cut(s)	1721, 1798, 2217
AsuNHI	GCTAGC	1 cut(s)	1753
AvaII	GGWCC	9 cut(s)	155, 246, 590, 727, 1663, 1799, 2164, 2201, 2281
AvrII	CCTAGG	1 cut(s)	359
AxyI	CCTNAGG	1 cut(s)	2172
BaeGI	GKGCMC	3 cut(s)	497, 1584, 2223
BalI	TGGCCA	1 cut(s)	1877
BamHI	GGATCC	1 cut(s)	2232
BanII	GRGCYC	4 cut(s)	171, 497, 744, 1343
BbsI	GAAGAC	1 cut(s)	134
Bbv12I	GWGCWC	3 cut(s)	171, 1584, 2223
BbvI	GCAGC	7 cut(s)	220, 274, 722, 1249, 1277, 1315, 2365
BccI	CCATC	2 cut(s)	191, 995
BceAI	ACGGC	1 cut(s)	1366
BciT130I	CCWGG	4 cut(s)	724, 923, 1760, 2205
BciVI	GTATCC	3 cut(s)	562, 607, 2032
BclI	TGATCA	2 cut(s)	316, 2121
BcnI	CCSGG	1 cut(s)	1472
BcoDI	GTCTC	8 cut(s)	389, 836, 1133, 1768, 1847, 1986, 2146, 2327
BfaI	CTAG	4 cut(s)	360, 414, 806, 1754
BfmI	CTRYAG	5 cut(s)	234, 1049, 1119, 1263, 2026
BfuAI	ACCTGC	2 cut(s)	227, 1865
BfuI	GTATCC	3 cut(s)	562, 607, 2032
BisI	GCNGC	9 cut(s)	66, 231, 234, 263, 711, 1263, 1266, 1329, 2379
BlnI	CCTAGG	1 cut(s)	359
BlpI	GCTNAGC	1 cut(s)	1386
BlsI	GCNGC	9 cut(s)	67, 232, 235, 264, 712, 1264, 1267, 1330, 2380
BmcAI	AGTACT	1 cut(s)	1616
Bme1390I	CCNGG	5 cut(s)	724, 923, 1472, 1760, 2205
Bme18I	GGWCC	9 cut(s)	155, 246, 590, 727, 1663, 1799, 2164, 2201, 2281
BmiI	GGNNCC	4 cut(s)	495, 728, 1664, 2234
BmrFI	CCNGG	5 cut(s)	724, 923, 1472, 1760, 2205
BmsI	GCATC	6 cut(s)	262, 527, 754, 1040, 1372, 2101
BmtI	GCTAGC	1 cut(s)	1757
BoxI	GACNNNNGTC	1 cut(s)	2319
BpiI	GAAGAC	1 cut(s)	134
BpmI	CTGGAG	1 cut(s)	1011
Bpu1102I	GCTNAGC	1 cut(s)	1386
BpuEI	CTTGAG	5 cut(s)	155, 1254, 1922, 2306, 2309
BpuMI	CCSGG	1 cut(s)	1472
BsaAI	YACGTR	1 cut(s)	795
BsaBI	GATNNNNATC	2 cut(s)	768, 2126
BsaI	GGTCTC	4 cut(s)	389, 1768, 1847, 2327
BsaWI	WCCGGW	1 cut(s)	1226
Bse1I	ACTGG	5 cut(s)	677, 730, 910, 1801, 1823
Bse21I	CCTNAGG	1 cut(s)	2172
Bse3DI	GCAATG	1 cut(s)	1524
Bse8I	GATNNNNATC	2 cut(s)	768, 2126
BseAI	TCCGGA	1 cut(s)	1226
BseBI	CCWGG	4 cut(s)	724, 923, 1760, 2205
BseJI	GATNNNNATC	2 cut(s)	768, 2126
BseMI	GCAATG	1 cut(s)	1524
BseMII	CTCAG	7 cut(s)	66, 1400, 1488, 1584, 2060, 2163, 2266
BseNI	ACTGG	5 cut(s)	677, 730, 910, 1801, 1823
BseSI	GKGCMC	3 cut(s)	497, 1584, 2223
BseX3I	CGGCCG	1 cut(s)	1351
BseXI	GCAGC	7 cut(s)	220, 274, 722, 1249, 1277, 1315, 2365
Bsh1285I	CGRYCG	1 cut(s)	1354
BshFI	GGCC	8 cut(s)	31, 125, 495, 551, 1019, 1353, 1877, 1973
BsiEI	CGRYCG	1 cut(s)	1354
BsiHKAI	GWGCWC	3 cut(s)	171, 1584, 2223
BsiSI	CCGG	2 cut(s)	1227, 1471
BslFI	GGGAC	2 cut(s)	467, 2190
BsmAI	GTCTC	8 cut(s)	389, 836, 1133, 1768, 1847, 1986, 2146, 2327
BsmFI	GGGAC	2 cut(s)	467, 2190
BsmI	GAATGC	2 cut(s)	1794, 2406
BsnI	GGCC	8 cut(s)	31, 125, 495, 551, 1019, 1353, 1877, 1973
Bso31I	GGTCTC	4 cut(s)	389, 1768, 1847, 2327
Bsp120I	GGGCCC	1 cut(s)	493
Bsp1286I	GDGCHC	6 cut(s)	171, 497, 744, 1343, 1584, 2223
Bsp13I	TCCGGA	1 cut(s)	1226
Bsp1720I	GCTNAGC	1 cut(s)	1386
Bsp19I	CCATGG	2 cut(s)	408, 1166
BspACI	CCGC	6 cut(s)	66, 148, 230, 744, 1910, 2114
BspANI	GGCC	8 cut(s)	31, 125, 495, 551, 1019, 1353, 1877, 1973
BspCNI	CTCAG	7 cut(s)	65, 1399, 1489, 1585, 2059, 2164, 2265
BspEI	TCCGGA	1 cut(s)	1226
BspLI	GGNNCC	4 cut(s)	495, 728, 1664, 2234
BspMAI	CTGCAG	4 cut(s)	238, 1053, 1123, 1267
BspMI	ACCTGC	2 cut(s)	227, 1865
BspOI	GCTAGC	1 cut(s)	1757
BspPI	GGATC	9 cut(s)	765, 1441, 1461, 1721, 1905, 1918, 2044, 2227, 2240
BspQI	GCTCTTC	2 cut(s)	197, 1551
BspTNI	GGTCTC	4 cut(s)	389, 1768, 1847, 2327
BsrDI	GCAATG	1 cut(s)	1524
BsrI	ACTGG	5 cut(s)	677, 730, 910, 1801, 1823
BssT1I	CCWWGG	7 cut(s)	180, 359, 408, 552, 1166, 1209, 2228
Bst2UI	CCWGG	4 cut(s)	724, 923, 1760, 2205
Bst4CI	ACNGT	5 cut(s)	1676, 1738, 1921, 2027, 2318
Bst6I	CTCTTC	3 cut(s)	197, 1456, 1551
BstAPI	GCANNNNNTGC	1 cut(s)	716
BstBAI	YACGTR	1 cut(s)	795
BstC8I	GCNNGC	8 cut(s)	63, 267, 744, 1053, 1173, 1755, 1941, 2355
BstDSI	CCRYGG	3 cut(s)	408, 1166, 1348
BstENI	CCTNNNNNAGG	1 cut(s)	2171
BstHHI	GCGC	1 cut(s)	1470
BstMAI	GTCTC	8 cut(s)	389, 836, 1133, 1768, 1847, 1986, 2146, 2327
BstMCI	CGRYCG	1 cut(s)	1354
BstMWI	GCNNNNNNNGC	7 cut(s)	387, 716, 1262, 1271, 1328, 1396, 1588
BstNI	CCWGG	4 cut(s)	724, 923, 1760, 2205
BstNSI	RCATGY	1 cut(s)	2065
BstPAI	GACNNNNGTC	1 cut(s)	2319
BstSCI	CCNGG	5 cut(s)	722, 921, 1470, 1758, 2203
BstSFI	CTRYAG	5 cut(s)	234, 1049, 1119, 1263, 2026
BstSLI	GKGCMC	3 cut(s)	497, 1584, 2223
BstSNI	TACGTA	1 cut(s)	795
BstV1I	GCAGC	7 cut(s)	220, 274, 722, 1249, 1277, 1315, 2365
BstV2I	GAAGAC	1 cut(s)	134
BstX2I	RGATCY	7 cut(s)	757, 1433, 1453, 1726, 1897, 2049, 2232
BstXI	CCANNNNNNTGG	2 cut(s)	121, 137
BstYI	RGATCY	7 cut(s)	757, 1433, 1453, 1726, 1897, 2049, 2232
BstZI	CGGCCG	1 cut(s)	1351
Bsu36I	CCTNAGG	1 cut(s)	2172
BsuI	GTATCC	3 cut(s)	562, 607, 2032
BsuRI	GGCC	8 cut(s)	31, 125, 495, 551, 1019, 1353, 1877, 1973
BtgI	CCRYGG	3 cut(s)	408, 1166, 1348
BtgZI	GCGATG	2 cut(s)	504, 1734
BtsI	GCAGTG	5 cut(s)	126, 214, 537, 1116, 1251
BtsIMutI	CAGTG	7 cut(s)	126, 214, 537, 1116, 1251, 1794, 2260
BveI	ACCTGC	2 cut(s)	227, 1865
Cac8I	GCNNGC	8 cut(s)	63, 267, 744, 1053, 1173, 1755, 1941, 2355
CfoI	GCGC	1 cut(s)	1470
CsiI	ACCWGGT	1 cut(s)	921
Csp6I	GTAC	8 cut(s)	81, 310, 353, 796, 874, 1371, 1615, 2386
CviAII	CATG	9 cut(s)	340, 409, 1167, 1606, 1732, 1879, 2062, 2107, 2368
CviQI	GTAC	8 cut(s)	81, 310, 353, 796, 874, 1371, 1615, 2386
DraI	TTTAAA	1 cut(s)	828
EaeI	YGGCCR	2 cut(s)	1351, 1875
EagI	CGGCCG	1 cut(s)	1351
Eam1104I	CTCTTC	3 cut(s)	197, 1456, 1551
EarI	CTCTTC	3 cut(s)	197, 1456, 1551
Ecl136II	GAGCTC	1 cut(s)	169
EclXI	CGGCCG	1 cut(s)	1351
Eco105I	TACGTA	1 cut(s)	795
Eco130I	CCWWGG	7 cut(s)	180, 359, 408, 552, 1166, 1209, 2228
Eco24I	GRGCYC	4 cut(s)	171, 497, 744, 1343
Eco31I	GGTCTC	4 cut(s)	389, 1768, 1847, 2327
Eco47I	GGWCC	9 cut(s)	155, 246, 590, 727, 1663, 1799, 2164, 2201, 2281
Eco52I	CGGCCG	1 cut(s)	1351
Eco53kI	GAGCTC	1 cut(s)	169
Eco57I	CTGAAG	6 cut(s)	888, 1355, 1535, 1996, 2201, 2364
Eco81I	CCTNAGG	1 cut(s)	2172
EcoICRI	GAGCTC	1 cut(s)	169
EcoNI	CCTNNNNNAGG	1 cut(s)	2171
EcoO109I	RGGNCCY	2 cut(s)	590, 2281
EcoRI	GAATTC	1 cut(s)	539
EcoRII	CCWGG	4 cut(s)	722, 921, 1758, 2203
EcoT14I	CCWWGG	7 cut(s)	180, 359, 408, 552, 1166, 1209, 2228
EcoT22I	ATGCAT	1 cut(s)	520
EcoT38I	GRGCYC	4 cut(s)	171, 497, 744, 1343
ErhI	CCWWGG	7 cut(s)	180, 359, 408, 552, 1166, 1209, 2228
FaeI	CATG	9 cut(s)	343, 412, 1170, 1609, 1735, 1882, 2065, 2110, 2371
FalI	AAGNNNNNCTT	2 cut(s)	164, 196
FaqI	GGGAC	2 cut(s)	467, 2190
FatI	CATG	9 cut(s)	339, 408, 1166, 1605, 1731, 1878, 2061, 2106, 2367
FauI	CCCGC	3 cut(s)	155, 751, 1903
FbaI	TGATCA	2 cut(s)	316, 2121
Fnu4HI	GCNGC	9 cut(s)	66, 231, 234, 263, 711, 1263, 1266, 1329, 2379
FriOI	GRGCYC	4 cut(s)	171, 497, 744, 1343
Fsp4HI	GCNGC	9 cut(s)	66, 231, 234, 263, 711, 1263, 1266, 1329, 2379
FspBI	CTAG	4 cut(s)	360, 414, 806, 1754
GlaI	GCGC	1 cut(s)	1469
GluI	GCNGC	9 cut(s)	66, 231, 234, 263, 711, 1263, 1266, 1329, 2379
GsuI	CTGGAG	1 cut(s)	1011
HaeIII	GGCC	8 cut(s)	31, 125, 495, 551, 1019, 1353, 1877, 1973
HapII	CCGG	2 cut(s)	1227, 1471
HhaI	GCGC	1 cut(s)	1470
Hin1II	CATG	9 cut(s)	343, 412, 1170, 1609, 1735, 1882, 2065, 2110, 2371
Hin6I	GCGC	1 cut(s)	1468
HinP1I	GCGC	1 cut(s)	1468
HincII	GTYRAC	1 cut(s)	2308
HindII	GTYRAC	1 cut(s)	2308
HindIII	AAGCTT	2 cut(s)	1272, 1547
HinfI	GANTC	4 cut(s)	185, 809, 1717, 1848
HpaI	GTTAAC	1 cut(s)	2308
HpaII	CCGG	2 cut(s)	1227, 1471
HphI	GGTGA	3 cut(s)	1721, 1798, 2217
Hpy166II	GTNNAC	6 cut(s)	682, 1582, 1647, 2005, 2221, 2308
Hpy8I	GTNNAC	6 cut(s)	682, 1582, 1647, 2005, 2221, 2308
HpyCH4III	ACNGT	5 cut(s)	1676, 1738, 1921, 2027, 2318
HpyCH4IV	ACGT	1 cut(s)	794
HpyF10VI	GCNNNNNNNGC	7 cut(s)	387, 716, 1262, 1271, 1328, 1396, 1588
HpySE526I	ACGT	1 cut(s)	794
Hsp92II	CATG	9 cut(s)	343, 412, 1170, 1609, 1735, 1882, 2065, 2110, 2371
HspAI	GCGC	1 cut(s)	1468
Kpn2I	TCCGGA	1 cut(s)	1226
Ksp22I	TGATCA	2 cut(s)	316, 2121
KspAI	GTTAAC	1 cut(s)	2308
LguI	GCTCTTC	2 cut(s)	197, 1551
LmnI	GCTCC	2 cut(s)	932, 1109
Lsp1109I	GCAGC	7 cut(s)	220, 274, 722, 1249, 1277, 1315, 2365
LweI	GCATC	6 cut(s)	262, 527, 754, 1040, 1372, 2101
MabI	ACCWGGT	1 cut(s)	921
MaeI	CTAG	4 cut(s)	360, 414, 806, 1754
MaeII	ACGT	1 cut(s)	794
MaeIII	GTNAC	6 cut(s)	859, 901, 1297, 1804, 2371, 2417
MfeI	CAATTG	1 cut(s)	773
MflI	RGATCY	7 cut(s)	757, 1433, 1453, 1726, 1897, 2049, 2232
MhlI	GDGCHC	6 cut(s)	171, 497, 744, 1343, 1584, 2223
MlsI	TGGCCA	1 cut(s)	1877
MluNI	TGGCCA	1 cut(s)	1877
MlyI	GAGTC	2 cut(s)	818, 1726
MmeI	TCCRAC	3 cut(s)	350, 1051, 1442
Mox20I	TGGCCA	1 cut(s)	1877
Mph1103I	ATGCAT	1 cut(s)	520
MroI	TCCGGA	1 cut(s)	1226
MscI	TGGCCA	1 cut(s)	1877
MseI	TTAA	7 cut(s)	57, 827, 954, 1161, 1533, 2277, 2307
MslI	CAYNNNNRTG	5 cut(s)	338, 1364, 1811, 2060, 2105
Msp20I	TGGCCA	1 cut(s)	1877
MspA1I	CMGCKG	2 cut(s)	265, 713
MspI	CCGG	2 cut(s)	1227, 1471
MspR9I	CCNGG	5 cut(s)	724, 923, 1472, 1760, 2205
MunI	CAATTG	1 cut(s)	773
Mva1269I	GAATGC	2 cut(s)	1794, 2406
MvaI	CCWGG	4 cut(s)	724, 923, 1760, 2205
MwoI	GCNNNNNNNGC	7 cut(s)	387, 716, 1262, 1271, 1328, 1396, 1588
NciI	CCSGG	1 cut(s)	1472
NcoI	CCATGG	2 cut(s)	408, 1166
NheI	GCTAGC	1 cut(s)	1753
NlaIII	CATG	9 cut(s)	343, 412, 1170, 1609, 1735, 1882, 2065, 2110, 2371
NlaIV	GGNNCC	4 cut(s)	495, 728, 1664, 2234
NmeAIII	GCCGAG	1 cut(s)	1999
NmuCI	GTSAC	2 cut(s)	1297, 1804
NsiI	ATGCAT	1 cut(s)	520
NspI	RCATGY	1 cut(s)	2065
OliI	CACNNNNGTG	1 cut(s)	1364
PasI	CCCWGGG	1 cut(s)	1759
PciI	ACATGT	1 cut(s)	2061
PciSI	GCTCTTC	2 cut(s)	197, 1551
PctI	GAATGC	2 cut(s)	1794, 2406
PfeI	GAWTC	2 cut(s)	185, 1848
PflMI	CCANNNNNTGG	2 cut(s)	255, 1659
PkrI	GCNGC	9 cut(s)	67, 232, 235, 264, 712, 1264, 1267, 1330, 2380
PleI	GAGTC	2 cut(s)	817, 1725
PpsI	GAGTC	2 cut(s)	817, 1725
Ppu21I	YACGTR	1 cut(s)	795
PpuMI	RGGWCCY	2 cut(s)	590, 2281
PscI	ACATGT	1 cut(s)	2061
PshAI	GACNNNNGTC	1 cut(s)	2319
Psp124BI	GAGCTC	1 cut(s)	171
Psp5II	RGGWCCY	2 cut(s)	590, 2281
Psp6I	CCWGG	4 cut(s)	722, 921, 1758, 2203
PspGI	CCWGG	4 cut(s)	722, 921, 1758, 2203
PspN4I	GGNNCC	4 cut(s)	495, 728, 1664, 2234
PspOMI	GGGCCC	1 cut(s)	493
PspPPI	RGGWCCY	2 cut(s)	590, 2281
PstI	CTGCAG	4 cut(s)	238, 1053, 1123, 1267
PsuI	RGATCY	7 cut(s)	757, 1433, 1453, 1726, 1897, 2049, 2232
PvuII	CAGCTG	2 cut(s)	265, 713
RsaI	GTAC	8 cut(s)	82, 311, 354, 797, 875, 1372, 1616, 2387
RsaNI	GTAC	8 cut(s)	81, 310, 353, 796, 874, 1371, 1615, 2386
RseI	CAYNNNNRTG	5 cut(s)	338, 1364, 1811, 2060, 2105
SacI	GAGCTC	1 cut(s)	171
SapI	GCTCTTC	2 cut(s)	197, 1551
SaqAI	TTAA	7 cut(s)	57, 827, 954, 1161, 1533, 2277, 2307
SatI	GCNGC	9 cut(s)	66, 231, 234, 263, 711, 1263, 1266, 1329, 2379
SbfI	CCTGCAGG	1 cut(s)	1053
ScaI	AGTACT	1 cut(s)	1616
SchI	GAGTC	2 cut(s)	818, 1726
ScrFI	CCNGG	5 cut(s)	724, 923, 1472, 1760, 2205
SdaI	CCTGCAGG	1 cut(s)	1053
SduI	GDGCHC	6 cut(s)	171, 497, 744, 1343, 1584, 2223
SexAI	ACCWGGT	1 cut(s)	921
SfaNI	GCATC	6 cut(s)	262, 527, 754, 1040, 1372, 2101
SfcI	CTRYAG	5 cut(s)	234, 1049, 1119, 1263, 2026
SinI	GGWCC	9 cut(s)	155, 246, 590, 727, 1663, 1799, 2164, 2201, 2281
SmiMI	CAYNNNNRTG	5 cut(s)	338, 1364, 1811, 2060, 2105
SmlI	CTYRAG	5 cut(s)	170, 1269, 1901, 2285, 2324
SmoI	CTYRAG	5 cut(s)	170, 1269, 1901, 2285, 2324
SnaBI	TACGTA	1 cut(s)	795
Sse8387I	CCTGCAGG	1 cut(s)	1053
SsiI	CCGC	6 cut(s)	66, 148, 230, 744, 1910, 2114
SspI	AATATT	1 cut(s)	2272
SspMI	CTAG	4 cut(s)	360, 414, 806, 1754
SstI	GAGCTC	1 cut(s)	171
StyD4I	CCNGG	5 cut(s)	722, 921, 1470, 1758, 2203
StyI	CCWWGG	7 cut(s)	180, 359, 408, 552, 1166, 1209, 2228
TaaI	ACNGT	5 cut(s)	1676, 1738, 1921, 2027, 2318
TaiI	ACGT	1 cut(s)	797
TaqI	TCGA	4 cut(s)	393, 762, 1851, 2130
TaqII	GACCGA	2 cut(s)	172, 2152
TatI	WGTACW	3 cut(s)	1370, 1614, 2385
TauI	GCSGC	2 cut(s)	68, 233
TfiI	GAWTC	2 cut(s)	185, 1848
Tru1I	TTAA	7 cut(s)	57, 827, 954, 1161, 1533, 2277, 2307
Tru9I	TTAA	7 cut(s)	57, 827, 954, 1161, 1533, 2277, 2307
TscAI	CASTG	7 cut(s)	126, 214, 537, 1123, 1258, 1801, 2260
TseFI	GTSAC	2 cut(s)	1297, 1804
TseI	GCWGC	7 cut(s)	233, 262, 710, 1262, 1265, 1328, 2378
Tsp45I	GTSAC	2 cut(s)	1297, 1804
TspDTI	ATGAA	6 cut(s)	17, 552, 1535, 1796, 1829, 1895
TspGWI	ACGGA	3 cut(s)	1027, 1599, 2261
TspRI	CASTG	7 cut(s)	126, 214, 537, 1123, 1258, 1801, 2260
Van91I	CCANNNNNTGG	2 cut(s)	255, 1659
VneI	GTGCAC	2 cut(s)	1580, 2219
VpaK11BI	GGWCC	9 cut(s)	155, 246, 590, 727, 1663, 1799, 2164, 2201, 2281
XagI	CCTNNNNNAGG	1 cut(s)	2171
XapI	RAATTY	2 cut(s)	539, 2074
XceI	RCATGY	1 cut(s)	2065
XmaJI	CCTAGG	1 cut(s)	359
XspI	CTAG	4 cut(s)	360, 414, 806, 1754
ZrmI	AGTACT	1 cut(s)	1616
Zsp2I	ATGCAT	1 cut(s)	520

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.