pycom11g25300

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0019765
Physical Location & Seq
Reverse (-)
28110481 .. 28111700
1220 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGGAGCGTGATCGATCTGCAATGTCAGAGAGAGAGAATGAGAATCTGGCGTCGGCGTCGGCGTCAGCGTCAGCTTCAGGGTCAAAGTTAAGGGCAGACGCGAAACCATACATACGAGGGTCGAACATCGGGTTTTCGTCGGAGGTACCTCTTCCTGCGACTCTGTGCACTTTACCGGTTTGTGACGGTGCGAGGATGCCAGCACCGGTGTTCGTACCTTGTCCGGATTCTTCTATAGAAGAAGTAGCTCCTGGAGGATATACGCCAGTGCTTCTCCCCCCAGATGAAAACTCAGCGCGGGCTTTTGTAAAGAATTTCATTTCGGTCCAATTGTCCAAGGTATCTGAGGAAGTTGATGAAAGTTTAGTTGATTATGTCAATGCATTTGACCCTTCTAAAGTTGCTGCTCAAGTGGAGGCTGTGCTATTTGAACGGTGGGGTTTCTTTGATTTGTCGAAACCAGTGAGTAGAAACTATTCGGTGGTTTTAAGTCGCCTGAGCAACGATGGTATCAAAGATTTCCGCAGACACATTCTTCTTGGAGAGATCTCGCCACAGACATTGTTCAACATGTCCTTAGATGAACTGTGGCAGAAGTACCTGCCCCCAAACAGAATGGGCGCTATGGTTTGGGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05016 map05016
Pfam Domains
Protein Families
Med26 TFIIS_C TFIIS_M

Protein Analysis

214

Amino Acids

23.47

Weight (kDa)

4.87

Isoelectric Point (pI)

59.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIS_M PF07500 97 - 198 3.9e-08 Transcription factor S-II (TFIIS), central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019765)

Species Orthologous Gene IDs
malus_domestica MD03G1264500.v1.1 MD03G1264600.v1.1 MD04G1039000.v1.1 MD11G1243600.v1.1 MD11G1250500.v1.1 MD11G1285500.v1.1
pyrus_communis pycom11g25300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 609
Acc65I GGTACC 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 145
AccII CGCG 2 cut(s) 101, 298
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 2 cut(s) 298, 523
AcsI RAATTY 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 60
AcyI GRCGYC 3 cut(s) 50, 56, 62
AfaI GTAC 3 cut(s) 147, 216, 599
AflIII ACRYGT 1 cut(s) 570
AgeI ACCGGT 2 cut(s) 175, 205
AgsI TTSAA 2 cut(s) 431, 568
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 2 cut(s) 74, 248
AluI AGCT 2 cut(s) 74, 248
Alw21I GWGCWC 1 cut(s) 170
Alw44I GTGCAC 1 cut(s) 166
Aor13HI TCCGGA 1 cut(s) 223
ApaLI GTGCAC 1 cut(s) 166
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 1 cut(s) 313
AsiGI ACCGGT 2 cut(s) 175, 205
Asp700I GAANNNNTTC 1 cut(s) 475
Asp718I GGTACC 1 cut(s) 145
AspLEI GCGC 2 cut(s) 298, 623
AspS9I GGNCC 1 cut(s) 325
AvaII GGWCC 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 170
BaeI ACNNNNGTAYC 2 cut(s) 493, 526
BanI GGYRCC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 391
BccI CCATC 1 cut(s) 500
BciT130I CCWGG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 234
BfoI RGCGCY 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 609
BglII AGATCT 1 cut(s) 546
BisI GCNGC 1 cut(s) 405
BlsI GCNGC 1 cut(s) 406
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 325
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 273
Bpu10I CCTNAGC 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 393
Bsa29I ATCGAT 1 cut(s) 13
BsaHI GRCGYC 3 cut(s) 50, 56, 62
BsaJI CCNNGG 1 cut(s) 336
BsaWI WCCGGW 3 cut(s) 175, 205, 223
Bse118I RCCGGY 2 cut(s) 175, 205
Bse1I ACTGG 2 cut(s) 266, 461
Bse3DI GCAATG 1 cut(s) 27
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 201
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 3 cut(s) 306, 336, 488
BseNI ACTGG 2 cut(s) 266, 461
BseSI GKGCMC 1 cut(s) 170
BseXI GCAGC 1 cut(s) 391
Bsh1236I CGCG 2 cut(s) 101, 298
BshNI GGYRCC 1 cut(s) 145
BshTI ACCGGT 2 cut(s) 175, 205
BshVI ATCGAT 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 3 cut(s) 176, 206, 224
Bsp1286I GDGCHC 1 cut(s) 170
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 3 cut(s) 10, 14, 546
BspACI CCGC 2 cut(s) 298, 523
BspCNI CTCAG 3 cut(s) 305, 337, 489
BspDI ATCGAT 1 cut(s) 13
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 2 cut(s) 101, 298
BspLI GGNNCC 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 609
BspT107I GGYRCC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 175, 205
BsrI ACTGG 2 cut(s) 266, 461
BssAI RCCGGY 2 cut(s) 175, 205
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 3 cut(s) 10, 14, 546
BssNI GRCGYC 3 cut(s) 50, 56, 62
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 188, 435, 588
Bst6I CTCTTC 1 cut(s) 156
BstACI GRCGYC 3 cut(s) 50, 56, 62
BstC8I GCNNGC 2 cut(s) 201, 300
BstDEI CTNAG 4 cut(s) 292, 345, 497, 577
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 2 cut(s) 101, 298
BstH2I RGCGCY 1 cut(s) 624
BstHHI GCGC 2 cut(s) 298, 623
BstKTI GATC 3 cut(s) 13, 17, 549
BstMBI GATC 3 cut(s) 10, 14, 546
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 574
BstSCI CCNGG 1 cut(s) 250
BstSFI CTRYAG 1 cut(s) 234
BstSLI GKGCMC 1 cut(s) 170
BstUI CGCG 2 cut(s) 101, 298
BstV1I GCAGC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
Bsu15I ATCGAT 1 cut(s) 13
BsuTUI ATCGAT 1 cut(s) 13
BtsCI GGATG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 273, 468
BveI ACCTGC 1 cut(s) 609
Cac8I GCNNGC 2 cut(s) 201, 300
CfoI GCGC 2 cut(s) 298, 623
Cfr10I RCCGGY 2 cut(s) 175, 205
Cfr13I GGNCC 1 cut(s) 325
ClaI ATCGAT 1 cut(s) 13
CseI GACGC 5 cut(s) 39, 45, 51, 57, 107
Csp6I GTAC 3 cut(s) 146, 215, 598
CspAI ACCGGT 2 cut(s) 175, 205
CspCI CAANNNNNGTGG 2 cut(s) 543, 578
CviAII CATG 1 cut(s) 571
CviJI RGCY 4 cut(s) 74, 248, 302, 419
CviKI_1 RGCY 4 cut(s) 74, 248, 302, 419
CviQI GTAC 3 cut(s) 146, 215, 598
DdeI CTNAG 4 cut(s) 292, 345, 497, 577
DpnI GATC 3 cut(s) 12, 16, 548
DpnII GATC 3 cut(s) 10, 14, 546
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 1 cut(s) 574
FaiI YATR 7 cut(s) 109, 113, 236, 261, 375, 572, 626
FatI CATG 1 cut(s) 570
FauI CCCGC 1 cut(s) 291
Fnu4HI GCNGC 1 cut(s) 405
FokI GGATG 1 cut(s) 208
Fsp4HI GCNGC 1 cut(s) 405
GlaI GCGC 2 cut(s) 297, 622
GluI GCNGC 1 cut(s) 405
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 624
HapII CCGG 3 cut(s) 176, 206, 224
HgaI GACGC 5 cut(s) 39, 45, 51, 57, 107
HhaI GCGC 2 cut(s) 298, 623
Hin1I GRCGYC 3 cut(s) 50, 56, 62
Hin1II CATG 1 cut(s) 574
Hin6I GCGC 2 cut(s) 296, 621
HinP1I GCGC 2 cut(s) 296, 621
HinfI GANTC 3 cut(s) 43, 160, 227
HpaII CCGG 3 cut(s) 176, 206, 224
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 3 cut(s) 28, 142, 346
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 1 cut(s) 168
Hpy99I CGWCG 3 cut(s) 55, 61, 142
HpyAV CCTTC 1 cut(s) 402
HpyCH4III ACNGT 3 cut(s) 188, 435, 588
HpyCH4V TGCA 3 cut(s) 20, 168, 383
HpyF3I CTNAG 4 cut(s) 292, 345, 497, 577
Hsp92I GRCGYC 3 cut(s) 50, 56, 62
Hsp92II CATG 1 cut(s) 574
HspAI GCGC 2 cut(s) 296, 621
Kpn2I TCCGGA 1 cut(s) 223
KpnI GGTACC 1 cut(s) 149
Kzo9I GATC 3 cut(s) 10, 14, 546
LmnI GCTCC 2 cut(s) 4, 253
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 182
MalI GATC 3 cut(s) 12, 16, 548
MboI GATC 3 cut(s) 10, 14, 546
MboII GAAGA 4 cut(s) 143, 222, 251, 527
MfeI CAATTG 1 cut(s) 329
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 2 cut(s) 313, 329
MlyI GAGTC 1 cut(s) 154
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 7 cut(s) 110, 136, 159, 186, 248, 340, 409
Mph1103I ATGCAT 1 cut(s) 385
MroI TCCGGA 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 475
MseI TTAA 2 cut(s) 89, 488
MspI CCGG 3 cut(s) 176, 206, 224
MspR9I CCNGG 1 cut(s) 252
MunI CAATTG 1 cut(s) 329
MvaI CCWGG 1 cut(s) 252
MvnI CGCG 2 cut(s) 101, 298
NdeII GATC 3 cut(s) 10, 14, 546
NlaIII CATG 1 cut(s) 574
NlaIV GGNNCC 1 cut(s) 147
NmuCI GTSAC 1 cut(s) 182
NsiI ATGCAT 1 cut(s) 385
NspI RCATGY 1 cut(s) 574
PciI ACATGT 1 cut(s) 570
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 65
PdmI GAANNNNTTC 1 cut(s) 475
PfeI GAWTC 2 cut(s) 43, 227
PfoI TCCNGGA 1 cut(s) 250
PinAI ACCGGT 2 cut(s) 175, 205
PkrI GCNGC 1 cut(s) 406
PleI GAGTC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 154
PscI ACATGT 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 147
PspPI GGNCC 1 cut(s) 325
PsuI RGATCY 1 cut(s) 546
RsaI GTAC 3 cut(s) 147, 216, 599
RsaNI GTAC 3 cut(s) 146, 215, 598
SaqAI TTAA 2 cut(s) 89, 488
SatI GCNGC 1 cut(s) 405
Sau3AI GATC 3 cut(s) 10, 14, 546
Sau96I GGNCC 1 cut(s) 325
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 1 cut(s) 170
SetI ASST 7 cut(s) 76, 147, 151, 220, 250, 342, 603
SfaNI GCATC 1 cut(s) 186
SfcI CTRYAG 1 cut(s) 234
SgrAI CRCCGGYG 1 cut(s) 205
SinI GGWCC 1 cut(s) 325
SmlI CTYRAG 1 cut(s) 408
SmoI CTYRAG 1 cut(s) 408
Sse9I AATT 2 cut(s) 313, 329
SsiI CCGC 2 cut(s) 298, 523
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 3 cut(s) 188, 435, 588
TaqI TCGA 3 cut(s) 13, 122, 455
TaqII GACCGA 1 cut(s) 313
TasI AATT 2 cut(s) 313, 329
TfiI GAWTC 2 cut(s) 43, 227
Tru1I TTAA 2 cut(s) 89, 488
Tru9I TTAA 2 cut(s) 89, 488
TscAI CASTG 2 cut(s) 273, 468
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 4 cut(s) 300, 307, 372, 597
TspRI CASTG 2 cut(s) 273, 468
VneI GTGCAC 1 cut(s) 166
VpaK11BI GGWCC 1 cut(s) 325
XapI RAATTY 1 cut(s) 313
XceI RCATGY 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 475
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.