pycom12397g00190

Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012397
OG0020180
Physical Location & Seq
Forward (+)
112562 .. 112990
429 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12397g00190.1

Sequence Viewer

Length: 429 bp
ATGACCAGAATTAAACGAGGGTATATAGCTCGAAGACGTAGAACAAAAATTCGTTTATTTGCATCAACCTTTCGAGGGGCTCATTCAAGACTTACTCGTACTATTACTCAACAGAAAATAAGAGCTTTGGTTTCGGCTCATCGGGATAGAGGTAGACAAAAGAGAGATTTTCGTCGGTTGTGGATCACTCGGATAAATGCAGTAATTCGCGAGAATAAAGTATACTTTAGTTATAGTAAATTTATACACAATCTGTACAAGAGACAGTTACTTCTTAATCGTAAAATACTTGCACAAATAGCTATATTAAATAAGAGTTGTCTTTATATGATTTCCAATGAGATCGATAAAATAAAGAGATTGGAAGGAATCCGTTGGAATAGTTTAAATGGAGTTCCCTGGAGAATGAACTCTGGGAAGGTAGAGTAG

Protein Analysis

143

Amino Acids

17.13

Weight (kDa)

11.87

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L20 PF00453 3 - 107 1.8e-31 Ribosomal protein L20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020180)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00660
malus_domestica MD11G1294300.v1.1
pyrus_communis pycom12397g00190
rosa_chinensis RchiOBHm_CPg0502001
rosa_roxburghii Rroxscaffold_2G00119030
rosa_wichuraiana Rw5G040380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 154, 222
AccII CGCG 1 cut(s) 210
AclWI GGATC 1 cut(s) 191
AcsI RAATTY 2 cut(s) 48, 239
AfaI GTAC 2 cut(s) 100, 257
AfiI CCNNNNNNNGG 1 cut(s) 75
AgsI TTSAA 1 cut(s) 87
AjnI CCWGG 1 cut(s) 398
AluBI AGCT 3 cut(s) 29, 125, 302
AluI AGCT 3 cut(s) 29, 125, 302
Alw26I GTCTC 1 cut(s) 256
AlwI GGATC 1 cut(s) 191
ApoI RAATTY 2 cut(s) 48, 239
BanII GRGCYC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 400
BmrFI CCNGG 1 cut(s) 400
BmsI GCATC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 421
Bsa29I ATCGAT 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 400
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 398
BseLI CCNNNNNNNGG 1 cut(s) 75
Bsh1236I CGCG 1 cut(s) 210
BshVI ATCGAT 1 cut(s) 345
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 82
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 2 cut(s) 183, 342
Bsp68I TCGCGA 1 cut(s) 210
BspDI ATCGAT 1 cut(s) 345
BspFNI CGCG 1 cut(s) 210
BspPI GGATC 1 cut(s) 191
BsrGI TGTACA 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 2 cut(s) 183, 342
BssNAI GTATAC 1 cut(s) 223
Bst1107I GTATAC 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 255
BstFNI CGCG 1 cut(s) 210
BstKTI GATC 2 cut(s) 186, 345
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 2 cut(s) 183, 342
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstNI CCWGG 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 398
BstUI CGCG 1 cut(s) 210
BstV2I GAAGAC 1 cut(s) 40
BstZ17I GTATAC 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 345
BsuTUI ATCGAT 1 cut(s) 345
BtuMI TCGCGA 1 cut(s) 210
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 2 cut(s) 99, 256
CviJI RGCY 5 cut(s) 29, 80, 125, 137, 302
CviKI_1 RGCY 5 cut(s) 29, 80, 125, 137, 302
CviQI GTAC 2 cut(s) 99, 256
DpnI GATC 2 cut(s) 185, 344
DpnII GATC 2 cut(s) 183, 342
DraI TTTAAA 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 82
FaiI YATR 8 cut(s) 24, 26, 223, 234, 245, 305, 327, 329
FblI GTMKAC 2 cut(s) 154, 222
FriOI GRGCYC 1 cut(s) 82
GsuI CTGGAG 1 cut(s) 421
HinfI GANTC 1 cut(s) 369
Hpy166II GTNNAC 2 cut(s) 155, 223
Hpy188I TCNGA 1 cut(s) 192
Hpy188III TCNNGA 3 cut(s) 87, 143, 209
Hpy8I GTNNAC 2 cut(s) 155, 223
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 2 cut(s) 359, 412
HpyCH4III ACNGT 1 cut(s) 267
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 3 cut(s) 62, 200, 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpySE526I ACGT 1 cut(s) 37
Kzo9I GATC 2 cut(s) 183, 342
LpnPI CCDG 4 cut(s) 19, 385, 399, 412
LweI GCATC 1 cut(s) 71
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 1 cut(s) 267
MalI GATC 2 cut(s) 185, 344
MboI GATC 2 cut(s) 183, 342
MboII GAAGA 1 cut(s) 45
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 4 cut(s) 9, 48, 204, 239
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 3 cut(s) 11, 68, 143
MseI TTAA 4 cut(s) 12, 276, 308, 386
MspR9I CCNGG 1 cut(s) 400
MvaI CCWGG 1 cut(s) 400
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 299
NdeII GATC 2 cut(s) 183, 342
NruI TCGCGA 1 cut(s) 210
PfeI GAWTC 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 398
PspGI CCWGG 1 cut(s) 398
RruI TCGCGA 1 cut(s) 210
RsaI GTAC 2 cut(s) 100, 257
RsaNI GTAC 2 cut(s) 99, 256
SaqAI TTAA 4 cut(s) 12, 276, 308, 386
Sau3AI GATC 2 cut(s) 183, 342
ScrFI CCNGG 1 cut(s) 400
SduI GDGCHC 1 cut(s) 82
SetI ASST 7 cut(s) 31, 40, 71, 127, 154, 304, 423
SfaNI GCATC 1 cut(s) 71
Sse9I AATT 4 cut(s) 9, 48, 204, 239
StyD4I CCNGG 1 cut(s) 398
TaaI ACNGT 1 cut(s) 267
TaiI ACGT 1 cut(s) 40
TaqI TCGA 3 cut(s) 31, 73, 345
TasI AATT 4 cut(s) 9, 48, 204, 239
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 1 cut(s) 369
Tru1I TTAA 4 cut(s) 12, 276, 308, 386
Tru9I TTAA 4 cut(s) 12, 276, 308, 386
TspDTI ATGAA 1 cut(s) 422
TspGWI ACGGA 1 cut(s) 362
XapI RAATTY 2 cut(s) 48, 239
XmiI GTMKAC 2 cut(s) 154, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.