pycom12397g00270

NDH shuttles electrons from NAD(P)H plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012397
OG0013283
Physical Location & Seq
Forward (+)
131512 .. 132192
681 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
ATGACTTCCATTATTGAATTTCCTTTACTTGATCGAACAACCCAAAATTCAGTTATTTCAACTACATCAAGTGATCTTTCAAATTGGTCAAGACTCTCCAGTTTATGGCCGCTTCTCTATGGTACCAGTTGTTGCTTCATTGAATTTGCTTCATTAATAGGCTCACGATTCGACTTTGATCGTTATGGACTGGTACCACGATCCAGTCCTAGACAGGCAGACCTAATTTTAACAGCAGGCACAGTAACAATGAAAATGGCTCCTTCTTTAGTAAGATTATATGAACAAATGCCTGAACCAAAATATGTTATTGCTATGGGAGCATGCACAATTACAGGGGGGATGTTCAGTACCGATTCTTATAGTACTGTTCGGGGAGTTGATAAGCTAATTCCTGTGGATGTCTATTTGCCGGGCTGTCCACCTAAACCGGAGGCCGTTATAGATGCTATAACAAAACTTCGTAAGAAAATTTCTCGAGAAATCTATGAAGATCGAATTAGATCTCAACAGGAAAATCGGTGTTTTACTACCAGCCATAAGTTTCGTGTTGGACGCAGTACTCATACTGGAAATTATGATCAAGGATTACTCTATCAACCACCGTCTACTTCAGAGATCCCTCCTGAAATATTTTTCAAATATAAAAGTTCAGTAGCTTCCCCCGAATTAGTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Oxidored_q4 Oxidored_q6

Protein Analysis

227

Amino Acids

25.35

Weight (kDa)

8.56

Isoelectric Point (pI)

55.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q6 PF01058 44 - 154 6.6e-29 NADH ubiquinone oxidoreductase, 20 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 122, 193
AccB1I GGYRCC 2 cut(s) 122, 193
AccB7I CCANNNNNTGG 1 cut(s) 105
AccI GTMKAC 1 cut(s) 608
AciI CCGC 1 cut(s) 110
AclWI GGATC 2 cut(s) 195, 613
AcoI YGGCCR 1 cut(s) 107
AcsI RAATTY 4 cut(s) 17, 46, 143, 471
AcuI CTGAAG 1 cut(s) 597
AfaI GTAC 5 cut(s) 124, 195, 352, 367, 562
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 5 cut(s) 17, 60, 81, 143, 640
AluBI AGCT 2 cut(s) 388, 659
AluI AGCT 2 cut(s) 388, 659
AlwI GGATC 2 cut(s) 195, 613
Ama87I CYCGRG 1 cut(s) 477
AoxI GGCC 2 cut(s) 107, 435
ApoI RAATTY 4 cut(s) 17, 46, 143, 471
AseI ATTAAT 1 cut(s) 155
Asp718I GGTACC 2 cut(s) 122, 193
AsuC2I CCSGG 1 cut(s) 414
AvaI CYCGRG 1 cut(s) 477
BanI GGYRCC 2 cut(s) 122, 193
BceAI ACGGC 1 cut(s) 422
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 414
BfaI CTAG 1 cut(s) 210
BglII AGATCT 1 cut(s) 503
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
BmcAI AGTACT 2 cut(s) 367, 562
Bme1390I CCNGG 1 cut(s) 414
BmeT110I CYCGRG 1 cut(s) 477
BmiI GGNNCC 3 cut(s) 124, 195, 261
BmrFI CCNGG 1 cut(s) 414
BmsI GCATC 1 cut(s) 436
BpmI CTGGAG 1 cut(s) 82
BpuMI CCSGG 1 cut(s) 414
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 5 cut(s) 99, 126, 195, 204, 574
BseGI GGATG 2 cut(s) 348, 406
BseLI CCNNNNNNNGG 1 cut(s) 105
BseNI ACTGG 5 cut(s) 99, 126, 195, 204, 574
BshFI GGCC 2 cut(s) 109, 437
BshNI GGYRCC 2 cut(s) 122, 193
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 2 cut(s) 413, 431
BslI CCNNNNNNNGG 1 cut(s) 105
BsnI GGCC 2 cut(s) 109, 437
BsoBI CYCGRG 1 cut(s) 477
Bsp143I GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
BspACI CCGC 1 cut(s) 110
BspANI GGCC 2 cut(s) 109, 437
BspLI GGNNCC 3 cut(s) 124, 195, 261
BspPI GGATC 2 cut(s) 195, 613
BspT107I GGYRCC 2 cut(s) 122, 193
BsrI ACTGG 5 cut(s) 99, 126, 195, 204, 574
BssMI GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
Bst4CI ACNGT 3 cut(s) 244, 370, 606
BstC8I GCNNGC 2 cut(s) 238, 325
BstF5I GGATG 2 cut(s) 348, 406
BstKTI GATC 8 cut(s) 34, 76, 181, 203, 496, 506, 583, 621
BstMBI GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNSI RCATGY 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 412
BstX2I RGATCY 2 cut(s) 503, 618
BstYI RGATCY 2 cut(s) 503, 618
BsuRI GGCC 2 cut(s) 109, 437
BtsCI GGATG 2 cut(s) 348, 406
Cac8I GCNNGC 2 cut(s) 238, 325
CseI GACGC 1 cut(s) 564
Csp6I GTAC 5 cut(s) 123, 194, 351, 366, 561
CviAII CATG 1 cut(s) 324
CviJI RGCY 8 cut(s) 109, 162, 260, 388, 417, 437, 537, 659
CviKI_1 RGCY 8 cut(s) 109, 162, 260, 388, 417, 437, 537, 659
CviQI GTAC 5 cut(s) 123, 194, 351, 366, 561
DpnI GATC 8 cut(s) 33, 75, 180, 202, 495, 505, 582, 620
DpnII GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
EaeI YGGCCR 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 477
FaeI CATG 1 cut(s) 327
FatI CATG 1 cut(s) 323
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 1 cut(s) 608
Fnu4HI GCNGC 1 cut(s) 110
FokI GGATG 2 cut(s) 355, 413
Fsp4HI GCNGC 1 cut(s) 110
FspBI CTAG 1 cut(s) 210
GluI GCNGC 1 cut(s) 110
GsuI CTGGAG 1 cut(s) 82
HaeIII GGCC 2 cut(s) 109, 437
HapII CCGG 2 cut(s) 413, 431
HgaI GACGC 1 cut(s) 564
Hin1II CATG 1 cut(s) 327
HinfI GANTC 3 cut(s) 93, 168, 356
HpaII CCGG 2 cut(s) 413, 431
Hpy166II GTNNAC 2 cut(s) 422, 609
Hpy188I TCNGA 1 cut(s) 616
Hpy188III TCNNGA 5 cut(s) 90, 165, 477, 479, 626
Hpy8I GTNNAC 2 cut(s) 422, 609
HpyAV CCTTC 1 cut(s) 273
HpyCH4III ACNGT 3 cut(s) 244, 370, 606
HpyCH4V TGCA 1 cut(s) 327
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
Hsp92II CATG 1 cut(s) 327
KpnI GGTACC 2 cut(s) 126, 197
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
LmnI GCTCC 2 cut(s) 265, 320
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 244
MalI GATC 8 cut(s) 33, 75, 180, 202, 495, 505, 582, 620
MboI GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
MboII GAAGA 1 cut(s) 503
MflI RGATCY 2 cut(s) 503, 618
MlyI GAGTC 1 cut(s) 87
MmeI TCCRAC 1 cut(s) 532
MnlI CCTC 2 cut(s) 427, 633
MseI TTAA 2 cut(s) 155, 230
MspI CCGG 2 cut(s) 413, 431
MspR9I CCNGG 1 cut(s) 414
MwoI GCNNNNNNNGC 1 cut(s) 320
NciI CCSGG 1 cut(s) 414
NdeII GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
NlaIII CATG 1 cut(s) 327
NlaIV GGNNCC 3 cut(s) 124, 195, 261
NspI RCATGY 1 cut(s) 327
PaeI GCATGC 1 cut(s) 327
PaeR7I CTCGAG 1 cut(s) 477
PfeI GAWTC 2 cut(s) 168, 356
PflMI CCANNNNNTGG 1 cut(s) 105
PkrI GCNGC 1 cut(s) 111
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 155
PspN4I GGNNCC 3 cut(s) 124, 195, 261
PsuI RGATCY 2 cut(s) 503, 618
RsaI GTAC 5 cut(s) 124, 195, 352, 367, 562
RsaNI GTAC 5 cut(s) 123, 194, 351, 366, 561
SaqAI TTAA 2 cut(s) 155, 230
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
ScaI AGTACT 2 cut(s) 367, 562
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 414
SetI ASST 4 cut(s) 225, 390, 427, 661
SfaNI GCATC 1 cut(s) 436
Sfr274I CTCGAG 1 cut(s) 477
SlaI CTCGAG 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 477
SmoI CTYRAG 1 cut(s) 477
SphI GCATGC 1 cut(s) 327
SsiI CCGC 1 cut(s) 110
SspI AATATT 1 cut(s) 633
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 412
TaaI ACNGT 3 cut(s) 244, 370, 606
TaqI TCGA 4 cut(s) 34, 171, 478, 496
TatI WGTACW 2 cut(s) 365, 560
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 2 cut(s) 168, 356
Tru1I TTAA 2 cut(s) 155, 230
Tru9I TTAA 2 cut(s) 155, 230
TspDTI ATGAA 5 cut(s) 127, 141, 266, 297, 504
Van91I CCANNNNNTGG 1 cut(s) 105
VspI ATTAAT 1 cut(s) 155
XapI RAATTY 4 cut(s) 17, 46, 143, 471
XceI RCATGY 1 cut(s) 327
XhoI CTCGAG 1 cut(s) 477
XmiI GTMKAC 1 cut(s) 608
XspI CTAG 1 cut(s) 210
ZrmI AGTACT 2 cut(s) 367, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.