pycom12401g00250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012401
OG0018760
Physical Location & Seq
Reverse (-)
146847 .. 148250
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12401g00250.5

Sequence Viewer

Length: 975 bp
ATGATAGAGGGCCATATCGTTGCGTTGGACAAGACGGCGCCGTCTCACTTCGAGTTCCTTCCTTCGTCGACAGAAGCAACGTTTCTTAGCGCGCTTTTCAAGCGCTTAGCTTCTGCTAGCGGCGGGCGGCAAGGCCATAAAGTCAGTTCAGGGAGCGGCTTTCTTGTGGTAGTAATTGCGAACATCTCTCCTTTTCTCTTAGCGTGCACAGTTTGCTTGCATGCCGCTTTAGGCACATCTCTCTTTCTTACGTATATAAACAGCTGTTTAGTGTATAAGCAATTCTTTAGTGGCCGCACCGAAAGGTACATACGGTGGAGGTTAGTTGAAGATGATGTTACGCGAAGTAAGGAAATGAGACGACTCTTTCTTGAACTATATCATAAACAGATCTTCCCCTCCACACCAATCACGAGTTTTTCTCCATTCCTCTCGTATATCGTCGTAACGCCCTTAATGCTAGGTTTTGAAAAAGACTTTTCATGTCATTCCCATTTAGGTTCGATTCGGATCCCTCCGTTGTTTCCTTTTCCTCCCGCACCTTTTTCTCGAAATGAGAAAGAAGATGGTACACTCGAATTGTATTATTTAAGTGCTTATTGCTTGCCAAAGATCCTACTTCTACAATTGGTAGGTCACCGGGTTATTCAAATAAGTTGTGTTTTCTGTGGTTTTCCCATGTTACAACTTCCGTACCAATTCAGTCGATCCGGAATGGATCGGTTAAACATTCTATTAGGGAGCCCGGTCTTGACTCTTCTGTGTGGTATTCATTCTCGTTCGGCTCTTGGAATCACATCCAGCAGTGGTTGGAACAGCTCGCAAAATCCAACCACTTTACCTACTTCATTGCCCCCAACCGTTTCTCGTACCTCTATTGAAACAGAATGGTTTCATGTTCTTTCATCGATTGGTTATTCCTCTCCGTTCGTATCTCTTTTTCCAATTTTGGTCTCGATTAGTTTACAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016021 GO:0031224 GO:0032991 GO:0043190 GO:0044425 GO:0044459 GO:0044464 GO:0071944 GO:0098533 GO:0098796 GO:0098797 GO:1902494 GO:1902495 GO:1904949 GO:1990351
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

36.01

Weight (kDa)

8.77

Isoelectric Point (pI)

68.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018760)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG00110
pyrus_communis pycom12401g00250
rosa_chinensis RchiOBHm_MTg0498291
rosa_laevigata RLG00000000353 RLG00000000380 RLG00000000396
rosa_roxburghii Rroxscaffold_2G00119410
rosa_samantha Rh1BG019600
rosa_wichuraiana Rw0G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 37
AccBSI CCGCTC 1 cut(s) 156
AccI GTMKAC 1 cut(s) 68
AccII CGCG 2 cut(s) 92, 343
AccIII TCCGGA 1 cut(s) 710
AciI CCGC 7 cut(s) 120, 123, 127, 156, 225, 295, 537
AclI AACGTT 1 cut(s) 80
AclWI GGATC 5 cut(s) 505, 518, 607, 702, 726
AcoI YGGCCR 1 cut(s) 292
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 4 cut(s) 308, 571, 695, 871
AfeI AGCGCT 1 cut(s) 104
AgsI TTSAA 6 cut(s) 100, 329, 374, 470, 650, 881
AluBI AGCT 3 cut(s) 110, 264, 819
AluI AGCT 3 cut(s) 110, 264, 819
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 3 cut(s) 48, 352, 958
Alw44I GTGCAC 1 cut(s) 205
AlwI GGATC 5 cut(s) 505, 518, 607, 702, 726
Aor13HI TCCGGA 1 cut(s) 710
Aor51HI AGCGCT 1 cut(s) 104
AoxI GGCC 3 cut(s) 10, 133, 292
ApaLI GTGCAC 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 891
AspLEI GCGC 4 cut(s) 40, 92, 94, 105
AspS9I GGNCC 1 cut(s) 10
AsuC2I CCSGG 2 cut(s) 641, 746
AsuHPI GGTGA 1 cut(s) 629
AsuNHI GCTAGC 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 209
BaeI ACNNNNGTAYC 2 cut(s) 677, 710
BamHI GGATCC 1 cut(s) 510
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 746
BauI CACGAG 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 209
BccI CCATC 1 cut(s) 560
BceAI ACGGC 2 cut(s) 25, 51
BcnI CCSGG 2 cut(s) 641, 746
BcoDI GTCTC 3 cut(s) 48, 352, 958
BfaI CTAG 2 cut(s) 117, 461
BfoI RGCGCY 2 cut(s) 41, 106
BglII AGATCT 1 cut(s) 390
BisI GCNGC 5 cut(s) 121, 128, 157, 225, 295
BlpI GCTNAGC 1 cut(s) 106
BlsI GCNGC 5 cut(s) 122, 129, 158, 226, 296
Bme1390I CCNGG 2 cut(s) 641, 746
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 3 cut(s) 39, 512, 743
BmrFI CCNGG 2 cut(s) 641, 746
BmtI GCTAGC 1 cut(s) 120
BplI GAGNNNNNCTC 2 cut(s) 406, 438
Bpu1102I GCTNAGC 1 cut(s) 106
BpuMI CCSGG 2 cut(s) 641, 746
Bsa29I ATCGAT 1 cut(s) 908
BsaAI YACGTR 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 509
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 958
BsaWI WCCGGW 1 cut(s) 710
Bse3DI GCAATG 1 cut(s) 848
Bse8I GATNNNNATC 1 cut(s) 509
BseAI TCCGGA 1 cut(s) 710
BseCI ATCGAT 1 cut(s) 908
BseGI GGATG 1 cut(s) 797
BseJI GATNNNNATC 1 cut(s) 509
BseMI GCAATG 1 cut(s) 848
BsePI GCGCGC 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 209
Bsh1236I CGCG 2 cut(s) 92, 343
BshFI GGCC 3 cut(s) 12, 135, 294
BshNI GGYRCC 1 cut(s) 37
BshVI ATCGAT 1 cut(s) 908
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 3 cut(s) 640, 711, 746
BsmAI GTCTC 3 cut(s) 48, 352, 958
BsmBI CGTCTC 2 cut(s) 48, 352
BsnI GGCC 3 cut(s) 12, 135, 294
Bso31I GGTCTC 1 cut(s) 958
Bsp1286I GDGCHC 2 cut(s) 209, 746
Bsp13I TCCGGA 1 cut(s) 710
Bsp143I GATC 5 cut(s) 390, 510, 612, 707, 718
Bsp1720I GCTNAGC 1 cut(s) 106
BspACI CCGC 7 cut(s) 120, 123, 127, 156, 225, 295, 537
BspANI GGCC 3 cut(s) 12, 135, 294
BspDI ATCGAT 1 cut(s) 908
BspEI TCCGGA 1 cut(s) 710
BspFNI CGCG 2 cut(s) 92, 343
BspLI GGNNCC 3 cut(s) 39, 512, 743
BspOI GCTAGC 1 cut(s) 120
BspPI GGATC 5 cut(s) 505, 518, 607, 702, 726
BspT107I GGYRCC 1 cut(s) 37
BspTNI GGTCTC 1 cut(s) 958
BsrBI CCGCTC 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 848
BssHII GCGCGC 1 cut(s) 90
BssMI GATC 5 cut(s) 390, 510, 612, 707, 718
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 412
Bst2BI CACGAG 1 cut(s) 412
Bst4CI ACNGT 3 cut(s) 211, 315, 862
Bst6I CTCTTC 1 cut(s) 762
BstACI GRCGYC 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 252
BstC8I GCNNGC 8 cut(s) 92, 118, 125, 205, 218, 222, 605, 821
BstDEI CTNAG 3 cut(s) 86, 106, 199
BstEII GGTNACC 1 cut(s) 635
BstF5I GGATG 1 cut(s) 797
BstFNI CGCG 2 cut(s) 92, 343
BstH2I RGCGCY 2 cut(s) 41, 106
BstHHI GCGC 4 cut(s) 40, 92, 94, 105
BstKTI GATC 5 cut(s) 393, 513, 615, 710, 721
BstMAI GTCTC 3 cut(s) 48, 352, 958
BstMBI GATC 5 cut(s) 390, 510, 612, 707, 718
BstMWI GCNNNNNNNGC 3 cut(s) 100, 213, 457
BstNSI RCATGY 1 cut(s) 224
BstPI GGTNACC 1 cut(s) 635
BstSCI CCNGG 2 cut(s) 639, 744
BstSLI GKGCMC 1 cut(s) 209
BstSNI TACGTA 1 cut(s) 252
BstUI CGCG 2 cut(s) 92, 343
BstX2I RGATCY 3 cut(s) 390, 510, 612
BstYI RGATCY 3 cut(s) 390, 510, 612
Bsu15I ATCGAT 1 cut(s) 908
BsuRI GGCC 3 cut(s) 12, 135, 294
BsuTUI ATCGAT 1 cut(s) 908
BtsCI GGATG 1 cut(s) 797
BtsI GCAGTG 1 cut(s) 811
BtsIMutI CAGTG 1 cut(s) 811
Cac8I GCNNGC 8 cut(s) 92, 118, 125, 205, 218, 222, 605, 821
CfoI GCGC 4 cut(s) 40, 92, 94, 105
Cfr13I GGNCC 1 cut(s) 10
ClaI ATCGAT 1 cut(s) 908
Csp6I GTAC 4 cut(s) 307, 570, 694, 870
CviAII CATG 4 cut(s) 221, 483, 679, 896
CviJI RGCY 9 cut(s) 12, 110, 135, 159, 264, 294, 744, 785, 819
CviKI_1 RGCY 9 cut(s) 12, 110, 135, 159, 264, 294, 744, 785, 819
CviQI GTAC 4 cut(s) 307, 570, 694, 870
DdeI CTNAG 3 cut(s) 86, 106, 199
DinI GGCGCC 1 cut(s) 39
DpnI GATC 5 cut(s) 392, 512, 614, 709, 720
DpnII GATC 5 cut(s) 390, 510, 612, 707, 718
DrdI GACNNNNNNGTC 1 cut(s) 40
DseDI GACNNNNNNGTC 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 762
EarI CTCTTC 1 cut(s) 762
Eco105I TACGTA 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 746
Eco31I GGTCTC 1 cut(s) 958
Eco47III AGCGCT 1 cut(s) 104
Eco91I GGTNACC 1 cut(s) 635
EcoO65I GGTNACC 1 cut(s) 635
EcoT38I GRGCYC 1 cut(s) 746
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
Esp3I CGTCTC 2 cut(s) 48, 352
FaeI CATG 4 cut(s) 224, 486, 682, 899
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FatI CATG 4 cut(s) 220, 482, 678, 895
FauI CCCGC 2 cut(s) 116, 544
FblI GTMKAC 1 cut(s) 68
Fnu4HI GCNGC 5 cut(s) 121, 128, 157, 225, 295
FokI GGATG 1 cut(s) 784
FriOI GRGCYC 1 cut(s) 746
Fsp4HI GCNGC 5 cut(s) 121, 128, 157, 225, 295
FspBI CTAG 2 cut(s) 117, 461
GlaI GCGC 4 cut(s) 39, 91, 93, 104
GluI GCNGC 5 cut(s) 121, 128, 157, 225, 295
HaeII RGCGCY 2 cut(s) 41, 106
HaeIII GGCC 3 cut(s) 12, 135, 294
HapII CCGG 3 cut(s) 640, 711, 746
HhaI GCGC 4 cut(s) 40, 92, 94, 105
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 4 cut(s) 224, 486, 682, 899
Hin6I GCGC 4 cut(s) 38, 90, 92, 103
HinP1I GCGC 4 cut(s) 38, 90, 92, 103
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 4 cut(s) 363, 505, 754, 792
HpaII CCGG 3 cut(s) 640, 711, 746
HphI GGTGA 1 cut(s) 629
Hpy166II GTNNAC 4 cut(s) 69, 207, 572, 965
Hpy188I TCNGA 1 cut(s) 510
Hpy188III TCNNGA 6 cut(s) 371, 412, 549, 711, 751, 955
Hpy8I GTNNAC 4 cut(s) 69, 207, 572, 965
Hpy99I CGWCG 2 cut(s) 70, 446
HpyAV CCTTC 2 cut(s) 68, 72
HpyCH4III ACNGT 3 cut(s) 211, 315, 862
HpyCH4IV ACGT 2 cut(s) 80, 251
HpyCH4V TGCA 2 cut(s) 207, 220
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 213, 457
HpyF3I CTNAG 3 cut(s) 86, 106, 199
HpySE526I ACGT 2 cut(s) 80, 251
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 4 cut(s) 224, 486, 682, 899
HspAI GCGC 4 cut(s) 38, 90, 92, 103
KasI GGCGCC 1 cut(s) 37
Kpn2I TCCGGA 1 cut(s) 710
Kzo9I GATC 5 cut(s) 390, 510, 612, 707, 718
LmnI GCTCC 2 cut(s) 153, 741
LpnPI CCDG 5 cut(s) 135, 653, 724, 759, 814
MaeI CTAG 2 cut(s) 117, 461
MaeII ACGT 2 cut(s) 80, 251
MaeIII GTNAC 4 cut(s) 337, 445, 635, 681
MalI GATC 5 cut(s) 392, 512, 614, 709, 720
MbiI CCGCTC 1 cut(s) 156
MboI GATC 5 cut(s) 390, 510, 612, 707, 718
MboII GAAGA 4 cut(s) 341, 385, 575, 749
MfeI CAATTG 1 cut(s) 626
MflI RGATCY 3 cut(s) 390, 510, 612
MhlI GDGCHC 2 cut(s) 209, 746
MluCI AATT 6 cut(s) 174, 281, 578, 626, 698, 945
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 2 cut(s) 357, 748
MmeI TCCRAC 3 cut(s) 6, 791, 854
MnlI CCTC 7 cut(s) 312, 409, 440, 525, 543, 883, 931
MroI TCCGGA 1 cut(s) 710
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 3 cut(s) 455, 590, 725
MspA1I CMGCKG 1 cut(s) 264
MspI CCGG 3 cut(s) 640, 711, 746
MspR9I CCNGG 2 cut(s) 641, 746
MunI CAATTG 1 cut(s) 626
MvnI CGCG 2 cut(s) 92, 343
MwoI GCNNNNNNNGC 3 cut(s) 100, 213, 457
NarI GGCGCC 1 cut(s) 38
NciI CCSGG 2 cut(s) 641, 746
NdeII GATC 5 cut(s) 390, 510, 612, 707, 718
NheI GCTAGC 1 cut(s) 116
NlaIII CATG 4 cut(s) 224, 486, 682, 899
NlaIV GGNNCC 3 cut(s) 39, 512, 743
NmuCI GTSAC 1 cut(s) 635
NspI RCATGY 1 cut(s) 224
PaeI GCATGC 1 cut(s) 224
PauI GCGCGC 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 2 cut(s) 505, 792
PkrI GCNGC 5 cut(s) 122, 129, 158, 226, 296
PleI GAGTC 2 cut(s) 357, 748
PluTI GGCGCC 1 cut(s) 41
PpsI GAGTC 2 cut(s) 357, 748
Ppu21I YACGTR 1 cut(s) 252
Psp1406I AACGTT 1 cut(s) 80
PspEI GGTNACC 1 cut(s) 635
PspN4I GGNNCC 3 cut(s) 39, 512, 743
PspPI GGNCC 1 cut(s) 10
PsuI RGATCY 3 cut(s) 390, 510, 612
PteI GCGCGC 1 cut(s) 90
PvuII CAGCTG 1 cut(s) 264
RsaI GTAC 4 cut(s) 308, 571, 695, 871
RsaNI GTAC 4 cut(s) 307, 570, 694, 870
SalI GTCGAC 1 cut(s) 67
SaqAI TTAA 3 cut(s) 455, 590, 725
SatI GCNGC 5 cut(s) 121, 128, 157, 225, 295
Sau3AI GATC 5 cut(s) 390, 510, 612, 707, 718
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 2 cut(s) 357, 748
ScrFI CCNGG 2 cut(s) 641, 746
SduI GDGCHC 2 cut(s) 209, 746
SfoI GGCGCC 1 cut(s) 39
SnaBI TACGTA 1 cut(s) 252
SphI GCATGC 1 cut(s) 224
Sse9I AATT 6 cut(s) 174, 281, 578, 626, 698, 945
SsiI CCGC 7 cut(s) 120, 123, 127, 156, 225, 295, 537
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 2 cut(s) 117, 461
StyD4I CCNGG 2 cut(s) 639, 744
TaaI ACNGT 3 cut(s) 211, 315, 862
TaiI ACGT 2 cut(s) 83, 254
TaqI TCGA 8 cut(s) 51, 68, 503, 550, 576, 706, 908, 956
TasI AATT 6 cut(s) 174, 281, 578, 626, 698, 945
TauI GCSGC 5 cut(s) 123, 130, 159, 227, 297
TfiI GAWTC 2 cut(s) 505, 792
Tru1I TTAA 3 cut(s) 455, 590, 725
Tru9I TTAA 3 cut(s) 455, 590, 725
TscAI CASTG 1 cut(s) 811
TseFI GTSAC 1 cut(s) 635
Tsp45I GTSAC 1 cut(s) 635
TspDTI ATGAA 5 cut(s) 471, 761, 837, 884, 894
TspGWI ACGGA 3 cut(s) 507, 681, 915
TspRI CASTG 1 cut(s) 811
VneI GTGCAC 1 cut(s) 205
XceI RCATGY 1 cut(s) 224
XmiI GTMKAC 1 cut(s) 68
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 2 cut(s) 117, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.