pycom12g01880

Transcriptional activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0018930
Physical Location & Seq
Reverse (-)
1554969 .. 1556133
1165 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 369 bp
ATGATGTGTGGCATCCGAGGACAAACAAATGACACATGTAGTTGGGAATTTGTGAGTTACATGCAAAGCACATGGAAAGAGCTTGAAGGGCTCTCAGATCAGATGAGGAAGGATGTTTCACAAGTTCGTAAGAAGATCGATGCGATTAACAAAGAGTTAAAGCCACTAGGACATACCTGCCAGAAGAAGGAAAAGGAGTACCGAGATGCACTTGAGTTTTTCAATGAAAAGAAGAGAGAAAAAGTGCAGCTTATCACAAAGCTAATGGAGGTTAGTTTGTTAAATAATTGTTTACTTGGCACAGCTAGGGTTGCAATTTCATGGATGGGTGGAACCCGAACTAGTCTGGCCAGTCCGTTGTTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04115 map04115
Pfam Domains
Protein Families
Transcrip_act

Protein Analysis

123

Amino Acids

14.06

Weight (kDa)

8.93

Isoelectric Point (pI)

16.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transcrip_act PF04949 22 - 91 1.3e-32 Transcriptional activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 1 cut(s) 47
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 187
AflIII ACRYGT 1 cut(s) 35
AgsI TTSAA 2 cut(s) 86, 223
AhlI ACTAGT 1 cut(s) 341
AluBI AGCT 4 cut(s) 82, 250, 262, 305
AluI AGCT 4 cut(s) 82, 250, 262, 305
AoxI GGCC 1 cut(s) 348
ApeKI GCWGC 1 cut(s) 247
ApoI RAATTY 1 cut(s) 47
BalI TGGCCA 1 cut(s) 350
BanII GRGCYC 1 cut(s) 93
BbvI GCAGC 1 cut(s) 259
BccI CCATC 1 cut(s) 319
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 3 cut(s) 167, 306, 342
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 1 cut(s) 248
BlsI GCNGC 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 334
BmsI GCATC 3 cut(s) 21, 130, 196
BpuEI CTTGAG 1 cut(s) 233
Bsa29I ATCGAT 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 16
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 351
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 16
BseGI GGATG 3 cut(s) 12, 118, 330
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 351
BseXI GCAGC 1 cut(s) 259
BsgI GTGCAG 1 cut(s) 266
BshFI GGCC 1 cut(s) 350
BshVI ATCGAT 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 187
BsnI GGCC 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 93
Bsp143I GATC 2 cut(s) 97, 135
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 1 cut(s) 107
BspDI ATCGAT 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 334
BspMI ACCTGC 1 cut(s) 185
BsrI ACTGG 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 16
BssMI GATC 2 cut(s) 97, 135
Bst6I CTCTTC 1 cut(s) 227
BstDEI CTNAG 1 cut(s) 94
BstF5I GGATG 3 cut(s) 12, 118, 330
BstKTI GATC 2 cut(s) 100, 138
BstMBI GATC 2 cut(s) 97, 135
BstMWI GCNNNNNNNGC 2 cut(s) 88, 311
BstNSI RCATGY 2 cut(s) 39, 64
BstV1I GCAGC 1 cut(s) 259
Bsu15I ATCGAT 1 cut(s) 138
BsuRI GGCC 1 cut(s) 350
BsuTUI ATCGAT 1 cut(s) 138
BtsCI GGATG 3 cut(s) 12, 118, 330
BveI ACCTGC 1 cut(s) 185
ClaI ATCGAT 1 cut(s) 138
Csp6I GTAC 1 cut(s) 199
CviAII CATG 4 cut(s) 36, 61, 72, 321
CviJI RGCY 7 cut(s) 82, 91, 163, 250, 262, 305, 350
CviKI_1 RGCY 7 cut(s) 82, 91, 163, 250, 262, 305, 350
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 1 cut(s) 94
DpnI GATC 2 cut(s) 99, 137
DpnII GATC 2 cut(s) 97, 135
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 4 cut(s) 39, 64, 75, 324
FaiI YATR 5 cut(s) 37, 62, 73, 174, 322
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FatI CATG 4 cut(s) 35, 60, 71, 320
Fnu4HI GCNGC 1 cut(s) 248
FokI GGATG 2 cut(s) 125, 337
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 248
FspBI CTAG 3 cut(s) 167, 306, 342
GluI GCNGC 1 cut(s) 248
HaeIII GGCC 1 cut(s) 350
Hin1II CATG 4 cut(s) 39, 64, 75, 324
Hpy166II GTNNAC 1 cut(s) 293
Hpy188I TCNGA 3 cut(s) 17, 97, 102
Hpy8I GTNNAC 1 cut(s) 293
HpyAV CCTTC 3 cut(s) 80, 103, 181
HpyCH4V TGCA 4 cut(s) 64, 209, 247, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 311
HpyF3I CTNAG 1 cut(s) 94
Hsp92II CATG 4 cut(s) 39, 64, 75, 324
Kzo9I GATC 2 cut(s) 97, 135
LpnPI CCDG 4 cut(s) 190, 194, 332, 364
Lsp1109I GCAGC 1 cut(s) 259
LweI GCATC 3 cut(s) 21, 130, 196
MaeI CTAG 3 cut(s) 167, 306, 342
MaeIII GTNAC 1 cut(s) 56
MalI GATC 2 cut(s) 99, 137
MboI GATC 2 cut(s) 97, 135
MboII GAAGA 3 cut(s) 145, 196, 244
MhlI GDGCHC 1 cut(s) 93
MlsI TGGCCA 1 cut(s) 350
MluCI AATT 3 cut(s) 47, 286, 315
MluNI TGGCCA 1 cut(s) 350
MnlI CCTC 3 cut(s) 11, 99, 262
Mox20I TGGCCA 1 cut(s) 350
MscI TGGCCA 1 cut(s) 350
MseI TTAA 3 cut(s) 147, 158, 281
Msp20I TGGCCA 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 88, 311
NdeII GATC 2 cut(s) 97, 135
NlaIII CATG 4 cut(s) 39, 64, 75, 324
NlaIV GGNNCC 1 cut(s) 334
NspI RCATGY 2 cut(s) 39, 64
PciI ACATGT 1 cut(s) 35
PkrI GCNGC 1 cut(s) 249
PscI ACATGT 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 334
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
SaqAI TTAA 3 cut(s) 147, 158, 281
SatI GCNGC 1 cut(s) 248
Sau3AI GATC 2 cut(s) 97, 135
SduI GDGCHC 1 cut(s) 93
SetI ASST 6 cut(s) 84, 179, 252, 264, 273, 307
SfaNI GCATC 3 cut(s) 21, 130, 196
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 3 cut(s) 47, 286, 315
SspMI CTAG 3 cut(s) 167, 306, 342
TaqI TCGA 1 cut(s) 138
TasI AATT 3 cut(s) 47, 286, 315
Tru1I TTAA 3 cut(s) 147, 158, 281
Tru9I TTAA 3 cut(s) 147, 158, 281
TseI GCWGC 1 cut(s) 247
TspDTI ATGAA 2 cut(s) 240, 309
TspGWI ACGGA 1 cut(s) 345
XapI RAATTY 1 cut(s) 47
XceI RCATGY 2 cut(s) 39, 64
XspI CTAG 3 cut(s) 167, 306, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.