pycom12g04820

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017505
Physical Location & Seq
Forward (+)
4743116 .. 4745931
2816 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1263 bp
ATGCAGTTTTCTTTTTCTAAAACGAAAAAGAAAAACCCCATTTTCGCGCCCAACAAAAAAAATCAAAAACCCTGCAGTCACACTCAACACTCTCGCGGTCTCACCCTCACCAAATCACTCCCACACTCCCGAAGAAGACCAGCAAAAAGCTGCAGATCTGATCAAAGAGCTCGTCATCCGCCTCCGCTCCCAAAACCCCAATTCCAAATCTCACCCGCCGACTCCAAACTCCCCGGAATTCCAGAGCTCGTTCAGCTACGCTTTTCCTTCAGCCGATTAACACCATCCGTGGCTCCCGACGCCGCCGCCATAGCCCAGTCCATCAAGTGCCGCCTCGCCACTCAAGGTAAGTCCTCGCAGGCCCTCACTTTCACCGGCCTTTATACCAAATTCGTGTCTAAAACTGGTCCCGGGAGTGTAAATAACAAATGGGCAGTCGTTTATTTGCTCAAAATTGTGTCGGATGATCGGAAAAATGGGAAAACCCAGTTGGATTCTTCGGTTTTGTTGCCCAATTTGGGGTTAAATGATGCCAAATCGGGGAAGGAGTTGAGGGTTTTGGTGGGTAGGGAGAATAAGGAAAAGGGTTGGAACAATGAGGTATTGTTGGTTTCTAAAGACCTGGAAAATCTTCGCGAAATTGCTTTTAGGGAGTTTGTGAAATTGATTAAAGAAAAAAATGAGGTTTCTGAAGAGGTTTTGGTGAGAGAGGTGTTGTATGCCTGTCAAGGAATTGATGGCAAGTATGTGAAATTTGATAATAGAGCTGATGGTTATGCTTTATCGGATTTGATTAAGGTGCCTAGAGCAACCCGCATTAGGGTTCAGAAGCTTTGTGAATTGGGGTGGTTGTTTAGGAAGGTTAAAGGGTATATTTCAGAGAGTACGGATGGGTTTCCATATGACAATGTTGGAACCGTTGGGCAGGCCTTTTGTGTTGCGTTACAAGATGAGCTCTCCGATTACTATAAGTTGTTGGCTGTTCTTGAAGTGCAATCAATGAATCCAATTCCTTTGGTTTCAGAGACTGCAAGCTCAGAGAATTATCTTTCTCTAAGGAGATTGTCTGTTTGGTTTGCGGAGCCAATGGTGAAAATGAGGTTAATGGCTGTTTTGGTTAATGAATGTAGGGTTTTAAGGGGTGGGGCAATGGCTAGGGCTATTCATTTACATGTCCAACATGGTAACCCATTGGTGCACGAGTTCATGGGACGCTTACTTCGGAGGGTATGTTCTCCACTATTTGAGATGGTGAGGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0008092 GO:0008150 GO:0008275 GO:0009605 GO:0009607 GO:0009624 GO:0009898 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032886 GO:0032991 GO:0033043 GO:0034622 GO:0043015 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044459 GO:0044464 GO:0046785 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051704 GO:0051707 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

421

Amino Acids

47.26

Weight (kDa)

9.85

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCP_N_terminal PF17681 235 - 419 3.2e-39 Gamma tubulin complex component N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06680
fragaria_vesca FvH4_6g21970
malus_domestica MD12G1055200.v1.1 MD14G1054400.v1.1
prunus_persica Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1 Prupe.7G080400_v2.0.a1
pyrus_communis pycom12g04820 pycom14g04490
rosa_laevigata RLG00000023588
rosa_roxburghii Rroxscaffold_6G00402450
rosa_samantha Rh3CG253200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 799
AccBSI CCGCTC 1 cut(s) 187
AccII CGCG 3 cut(s) 47, 96, 636
AciI CCGC 9 cut(s) 96, 179, 185, 216, 303, 306, 331, 814, 1079
AcsI RAATTY 3 cut(s) 237, 389, 752
AcuI CTGAAG 2 cut(s) 253, 711
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 1 cut(s) 886
AfiI CCNNNNNNNGG 5 cut(s) 518, 519, 540, 819, 820
AflIII ACRYGT 1 cut(s) 1171
AgsI TTSAA 1 cut(s) 989
AjnI CCWGG 1 cut(s) 621
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AloI GAACNNNNNNTCC 2 cut(s) 1216, 1248
AluBI AGCT 8 cut(s) 150, 170, 247, 256, 767, 832, 955, 1035
AluI AGCT 8 cut(s) 150, 170, 247, 256, 767, 832, 955, 1035
Alw21I GWGCWC 4 cut(s) 172, 249, 957, 1200
Alw26I GTCTC 2 cut(s) 104, 1019
Alw44I GTGCAC 1 cut(s) 1196
AlwNI CAGNNNCTG 1 cut(s) 1028
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 3 cut(s) 360, 376, 927
ApaLI GTGCAC 1 cut(s) 1196
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 3 cut(s) 237, 389, 752
ArsI GACNNNNNNTTYG 6 cut(s) 157, 189, 420, 452, 1203, 1235
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 361, 407
AsuC2I CCSGG 3 cut(s) 234, 411, 412
AsuHPI GGTGA 6 cut(s) 94, 100, 204, 364, 715, 1102
AvaI CYCGRG 1 cut(s) 410
AvaII GGWCC 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 1200
BanI GGYRCC 1 cut(s) 799
BanII GRGCYC 3 cut(s) 172, 249, 957
BauI CACGAG 1 cut(s) 1199
BbsI GAAGAC 1 cut(s) 142
Bbv12I GWGCWC 4 cut(s) 172, 249, 957, 1200
BbvI GCAGC 1 cut(s) 137
BccI CCATC 6 cut(s) 292, 329, 731, 764, 884, 1243
BciT130I CCWGG 1 cut(s) 623
BclI TGATCA 1 cut(s) 160
BcnI CCSGG 3 cut(s) 234, 411, 412
BcoDI GTCTC 2 cut(s) 104, 1019
BfaI CTAG 2 cut(s) 804, 1155
BfmI CTRYAG 2 cut(s) 73, 151
BglII AGATCT 1 cut(s) 155
BisI GCNGC 4 cut(s) 151, 303, 306, 331
BlsI GCNGC 4 cut(s) 152, 304, 307, 332
Bme1390I CCNGG 4 cut(s) 234, 411, 412, 623
Bme18I GGWCC 1 cut(s) 407
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 361, 407
BmiI GGNNCC 5 cut(s) 294, 409, 801, 916, 1083
BmrFI CCNGG 4 cut(s) 234, 411, 412, 623
BmrI ACTGGG 2 cut(s) 310, 481
BmsI GCATC 1 cut(s) 520
BmuI ACTGGG 2 cut(s) 310, 481
BpiI GAAGAC 1 cut(s) 142
BpuEI CTTGAG 1 cut(s) 327
BpuMI CCSGG 3 cut(s) 234, 411, 412
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 3 cut(s) 232, 288, 410
BsaXI ACNNNNNCTCC 2 cut(s) 1216, 1246
Bsc4I CCNNNNNNNGG 5 cut(s) 518, 519, 540, 819, 820
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 3 cut(s) 316, 409, 487
Bse3DI GCAATG 1 cut(s) 1155
BseBI CCWGG 1 cut(s) 623
BseDI CCNNGG 3 cut(s) 232, 288, 410
BseGI GGATG 4 cut(s) 175, 284, 469, 895
BseLI CCNNNNNNNGG 5 cut(s) 518, 519, 540, 819, 820
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 1 cut(s) 1050
BseNI ACTGG 3 cut(s) 316, 409, 487
BseSI GKGCMC 1 cut(s) 1200
BseXI GCAGC 1 cut(s) 137
Bsh1236I CGCG 3 cut(s) 47, 96, 636
BshFI GGCC 3 cut(s) 362, 378, 929
BshNI GGYRCC 1 cut(s) 799
BsiHKAI GWGCWC 4 cut(s) 172, 249, 957, 1200
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 3 cut(s) 234, 375, 411
BslFI GGGAC 2 cut(s) 393, 1224
BslI CCNNNNNNNGG 5 cut(s) 518, 519, 540, 819, 820
BsmAI GTCTC 2 cut(s) 104, 1019
BsmFI GGGAC 2 cut(s) 393, 1224
BsnI GGCC 3 cut(s) 362, 378, 929
Bso31I GGTCTC 1 cut(s) 104
BsoBI CYCGRG 1 cut(s) 410
Bsp1286I GDGCHC 4 cut(s) 172, 249, 957, 1200
Bsp143I GATC 3 cut(s) 155, 160, 466
Bsp68I TCGCGA 1 cut(s) 636
BspACI CCGC 9 cut(s) 96, 179, 185, 216, 303, 306, 331, 814, 1079
BspANI GGCC 3 cut(s) 362, 378, 929
BspCNI CTCAG 1 cut(s) 1049
BspFNI CGCG 3 cut(s) 47, 96, 636
BspLI GGNNCC 5 cut(s) 294, 409, 801, 916, 1083
BspMAI CTGCAG 2 cut(s) 77, 155
BspT107I GGYRCC 1 cut(s) 799
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 187
BsrDI GCAATG 1 cut(s) 1155
BsrFI RCCGGY 1 cut(s) 374
BsrI ACTGG 3 cut(s) 316, 409, 487
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 232, 288, 410
BssMI GATC 3 cut(s) 155, 160, 466
BssNI GRCGYC 1 cut(s) 300
BssSI CACGAG 1 cut(s) 1199
Bst2BI CACGAG 1 cut(s) 1199
Bst2UI CCWGG 1 cut(s) 623
Bst4CI ACNGT 1 cut(s) 919
Bst6I CTCTTC 1 cut(s) 687
BstACI GRCGYC 1 cut(s) 300
BstC8I GCNNGC 3 cut(s) 360, 927, 1033
BstDEI CTNAG 2 cut(s) 1036, 1055
BstDSI CCRYGG 1 cut(s) 288
BstEII GGTNACC 1 cut(s) 1184
BstF5I GGATG 4 cut(s) 175, 284, 469, 895
BstFNI CGCG 3 cut(s) 47, 96, 636
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 3 cut(s) 158, 163, 469
BstMAI GTCTC 2 cut(s) 104, 1019
BstMBI GATC 3 cut(s) 155, 160, 466
BstMWI GCNNNNNNNGC 3 cut(s) 253, 299, 311
BstNI CCWGG 1 cut(s) 623
BstNSI RCATGY 1 cut(s) 1175
BstPI GGTNACC 1 cut(s) 1184
BstSCI CCNGG 4 cut(s) 232, 409, 410, 621
BstSFI CTRYAG 2 cut(s) 73, 151
BstSLI GKGCMC 1 cut(s) 1200
BstUI CGCG 3 cut(s) 47, 96, 636
BstV1I GCAGC 1 cut(s) 137
BstV2I GAAGAC 1 cut(s) 142
BstX2I RGATCY 1 cut(s) 155
BstYI RGATCY 1 cut(s) 155
BsuRI GGCC 3 cut(s) 362, 378, 929
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 4 cut(s) 175, 284, 469, 895
BtuMI TCGCGA 1 cut(s) 636
Cac8I GCNNGC 3 cut(s) 360, 927, 1033
CaiI CAGNNNCTG 1 cut(s) 1028
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 2 cut(s) 361, 407
Cfr9I CCCGGG 1 cut(s) 410
CseI GACGC 2 cut(s) 308, 1221
Csp6I GTAC 1 cut(s) 885
CviAII CATG 3 cut(s) 1172, 1181, 1207
CviQI GTAC 1 cut(s) 885
DdeI CTNAG 2 cut(s) 1036, 1055
DpnI GATC 3 cut(s) 157, 162, 468
DpnII GATC 3 cut(s) 155, 160, 466
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
EciI GGCGGA 1 cut(s) 168
Ecl136II GAGCTC 3 cut(s) 170, 247, 955
Eco147I AGGCCT 1 cut(s) 929
Eco24I GRGCYC 3 cut(s) 172, 249, 957
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 407
Eco53kI GAGCTC 3 cut(s) 170, 247, 955
Eco57I CTGAAG 2 cut(s) 253, 711
Eco88I CYCGRG 1 cut(s) 410
Eco91I GGTNACC 1 cut(s) 1184
EcoICRI GAGCTC 3 cut(s) 170, 247, 955
EcoO109I RGGNCCY 1 cut(s) 361
EcoO65I GGTNACC 1 cut(s) 1184
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 621
EcoT38I GRGCYC 3 cut(s) 172, 249, 957
FaeI CATG 3 cut(s) 1175, 1184, 1210
FaqI GGGAC 2 cut(s) 393, 1224
FatI CATG 3 cut(s) 1171, 1180, 1206
FauI CCCGC 2 cut(s) 223, 821
FauNDI CATATG 1 cut(s) 901
FbaI TGATCA 1 cut(s) 160
Fnu4HI GCNGC 4 cut(s) 151, 303, 306, 331
FokI GGATG 4 cut(s) 162, 271, 476, 902
FriOI GRGCYC 3 cut(s) 172, 249, 957
Fsp4HI GCNGC 4 cut(s) 151, 303, 306, 331
FspBI CTAG 2 cut(s) 804, 1155
GlaI GCGC 1 cut(s) 48
GluI GCNGC 4 cut(s) 151, 303, 306, 331
HaeIII GGCC 3 cut(s) 362, 378, 929
HapII CCGG 3 cut(s) 234, 375, 411
HgaI GACGC 2 cut(s) 308, 1221
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 3 cut(s) 1175, 1184, 1210
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 3 cut(s) 221, 494, 1003
HpaII CCGG 3 cut(s) 234, 375, 411
HphI GGTGA 6 cut(s) 94, 100, 204, 364, 715, 1102
Hpy166II GTNNAC 1 cut(s) 1198
Hpy188III TCNNGA 5 cut(s) 129, 242, 296, 635, 986
Hpy8I GTNNAC 1 cut(s) 1198
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 3 cut(s) 277, 538, 853
HpyCH4III ACNGT 1 cut(s) 919
HpyCH4V TGCA 6 cut(s) 4, 75, 153, 994, 1031, 1198
HpyF10VI GCNNNNNNNGC 3 cut(s) 253, 299, 311
HpyF3I CTNAG 2 cut(s) 1036, 1055
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 3 cut(s) 1175, 1184, 1210
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 3 cut(s) 155, 160, 466
LmnI GCTCC 3 cut(s) 192, 298, 1081
Lsp1109I GCAGC 1 cut(s) 137
LweI GCATC 1 cut(s) 520
MaeI CTAG 2 cut(s) 804, 1155
MaeIII GTNAC 3 cut(s) 77, 942, 1184
MalI GATC 3 cut(s) 157, 162, 468
MbiI CCGCTC 1 cut(s) 187
MboI GATC 3 cut(s) 155, 160, 466
MboII GAAGA 5 cut(s) 144, 147, 489, 623, 704
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 4 cut(s) 172, 249, 957, 1200
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 5 cut(s) 441, 471, 569, 892, 1201
MroXI GAANNNNTTC 1 cut(s) 630
MseI TTAA 8 cut(s) 278, 524, 669, 795, 864, 1103, 1119, 1136
MslI CAYNNNNRTG 1 cut(s) 1170
MspI CCGG 3 cut(s) 234, 375, 411
MspR9I CCNGG 4 cut(s) 234, 411, 412, 623
MvaI CCWGG 1 cut(s) 623
MvnI CGCG 3 cut(s) 47, 96, 636
MwoI GCNNNNNNNGC 3 cut(s) 253, 299, 311
NciI CCSGG 3 cut(s) 234, 411, 412
NdeI CATATG 1 cut(s) 901
NdeII GATC 3 cut(s) 155, 160, 466
NlaIII CATG 3 cut(s) 1175, 1184, 1210
NlaIV GGNNCC 5 cut(s) 294, 409, 801, 916, 1083
NmuCI GTSAC 1 cut(s) 77
NruI TCGCGA 1 cut(s) 636
NspI RCATGY 1 cut(s) 1175
PceI AGGCCT 1 cut(s) 929
PciI ACATGT 1 cut(s) 1171
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 494, 1003
PkrI GCNGC 4 cut(s) 152, 304, 307, 332
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
PscI ACATGT 1 cut(s) 1171
Psp124BI GAGCTC 3 cut(s) 172, 249, 957
Psp6I CCWGG 1 cut(s) 621
PspEI GGTNACC 1 cut(s) 1184
PspGI CCWGG 1 cut(s) 621
PspN4I GGNNCC 5 cut(s) 294, 409, 801, 916, 1083
PspPI GGNCC 2 cut(s) 361, 407
PstI CTGCAG 2 cut(s) 77, 155
PstNI CAGNNNCTG 1 cut(s) 1028
PsuI RGATCY 1 cut(s) 155
RruI TCGCGA 1 cut(s) 636
RsaI GTAC 1 cut(s) 886
RsaNI GTAC 1 cut(s) 885
RseI CAYNNNNRTG 1 cut(s) 1170
SacI GAGCTC 3 cut(s) 172, 249, 957
SaqAI TTAA 8 cut(s) 278, 524, 669, 795, 864, 1103, 1119, 1136
SatI GCNGC 4 cut(s) 151, 303, 306, 331
Sau3AI GATC 3 cut(s) 155, 160, 466
Sau96I GGNCC 2 cut(s) 361, 407
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 4 cut(s) 234, 411, 412, 623
SduI GDGCHC 4 cut(s) 172, 249, 957, 1200
SfaNI GCATC 1 cut(s) 520
SfcI CTRYAG 2 cut(s) 73, 151
SinI GGWCC 1 cut(s) 407
SmaI CCCGGG 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 1170
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
SseBI AGGCCT 1 cut(s) 929
SsiI CCGC 9 cut(s) 96, 179, 185, 216, 303, 306, 331, 814, 1079
SspMI CTAG 2 cut(s) 804, 1155
SstI GAGCTC 3 cut(s) 172, 249, 957
StuI AGGCCT 1 cut(s) 929
StyD4I CCNGG 4 cut(s) 232, 409, 410, 621
TaaI ACNGT 1 cut(s) 919
TauI GCSGC 3 cut(s) 305, 308, 333
TfiI GAWTC 2 cut(s) 494, 1003
Tru1I TTAA 8 cut(s) 278, 524, 669, 795, 864, 1103, 1119, 1136
Tru9I TTAA 8 cut(s) 278, 524, 669, 795, 864, 1103, 1119, 1136
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 4 cut(s) 1016, 1137, 1154, 1195
TspGWI ACGGA 2 cut(s) 277, 902
TspMI CCCGGG 1 cut(s) 410
VneI GTGCAC 1 cut(s) 1196
VpaK11BI GGWCC 1 cut(s) 407
XapI RAATTY 3 cut(s) 237, 389, 752
XceI RCATGY 1 cut(s) 1175
XmaI CCCGGG 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 2 cut(s) 804, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.