pycom12g10930

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015683
Physical Location & Seq
Forward (+)
13040137 .. 13041844
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
ATGGCATACATTAACCCTTCCTACATCGAAATTAGTGACGACGACGACCAAATCCAACAGGAGCAACACCCAGAGCTGCCGATCAACGATCATCCAGAACACGAAAACGCAGACGACGACTTAGACTATGAACAGTACCTTGCCGTACTGCTAGCCTCTAGCTTCTCTCAAAATGCTGGCGGAACTGATTCCGGCGACGACGATGGGTTCGGTGTTCCTCCAAGTGCTGCCGAAATTGCTGACCAAACAGATTTTGGCGACGAGACTGTGGAAGCTGATTTTTTTAATGTTGCCGCAATTGCCGGCGAAACGGAACAGTCAGGTGGGGCCGAGGAAGGCGGAGAGAAGCGGAGAAGAATAGGAGGGCCCGAAACGTCGGCGGCGGCGGGTGGAATTGGCAGCAAAAGGGGCTTGATTTGCTCTATTTGCTTAGATTCTAGGACCGTTGAGGGTGATCACCGCATCTGTTCTCTTCCTTGTGGGCACCTATTCGGGATGTCTTGCATAAACAGATGGCTACAGCATAAAGATACCGGGAAGTGTCCTATATGCAACCGAAAATGCAAGTTGAAGGATGTTTTGAAACTTTTCGTATCATATCCATGGGATGATTCTGTTGATGATGATGAATTAGAAAAGCAAAAGACGATTCGGGAACTCAAGGATAAATGTGCTTCTCTTGAGAATGAGCTTGCTGCGTTGCGCAAGAACGAAGCTAAACTGCAGCTTAAAGATGAACAACGTACGAAGGGTATGGAAGAAAAATTCGCTGATCAACTTGCTCGTAGTTTGGATACAGATAGAAATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

271

Amino Acids

29.78

Weight (kDa)

4.49

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 140 - 185 1.7e-10 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 483
AciI CCGC 8 cut(s) 180, 294, 339, 349, 380, 383, 386, 460
AcsI RAATTY 2 cut(s) 764, 805
AfaI GTAC 3 cut(s) 137, 147, 745
AgsI TTSAA 2 cut(s) 571, 583
AluBI AGCT 6 cut(s) 76, 162, 275, 691, 716, 727
AluI AGCT 6 cut(s) 76, 162, 275, 691, 716, 727
Alw26I GTCTC 1 cut(s) 257
AoxI GGCC 2 cut(s) 327, 365
ApaI GGGCCC 1 cut(s) 369
ApeKI GCWGC 5 cut(s) 76, 227, 399, 695, 724
ApoI RAATTY 2 cut(s) 764, 805
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 2 cut(s) 187, 587
AspLEI GCGC 1 cut(s) 705
AspS9I GGNCC 4 cut(s) 327, 365, 366, 441
AsuC2I CCSGG 1 cut(s) 535
AsuHPI GGTGA 2 cut(s) 449, 464
AsuNHI GCTAGC 1 cut(s) 151
AvaII GGWCC 1 cut(s) 441
BaeGI GKGCMC 2 cut(s) 369, 486
BanI GGYRCC 1 cut(s) 483
BanII GRGCYC 1 cut(s) 369
BbvI GCAGC 5 cut(s) 63, 214, 411, 682, 736
BccI CCATC 2 cut(s) 197, 507
BceAI ACGGC 1 cut(s) 128
BciVI GTATCC 1 cut(s) 787
BclI TGATCA 2 cut(s) 454, 772
BcnI CCSGG 1 cut(s) 535
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 3 cut(s) 152, 159, 438
BfmI CTRYAG 2 cut(s) 518, 722
BfuI GTATCC 1 cut(s) 787
BisI GCNGC 8 cut(s) 77, 228, 294, 381, 384, 400, 696, 725
BlsI GCNGC 8 cut(s) 78, 229, 295, 382, 385, 401, 697, 726
Bme1390I CCNGG 1 cut(s) 535
Bme18I GGWCC 1 cut(s) 441
BmgT120I GGNCC 4 cut(s) 327, 365, 366, 441
BmiI GGNNCC 3 cut(s) 328, 367, 485
BmrFI CCNGG 1 cut(s) 535
BmsI GCATC 1 cut(s) 471
BmtI GCTAGC 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 644, 701
BpuMI CCSGG 1 cut(s) 535
BsaJI CCNNGG 2 cut(s) 330, 602
Bse118I RCCGGY 1 cut(s) 302
BseDI CCNNGG 2 cut(s) 330, 602
BseGI GGATG 4 cut(s) 91, 501, 580, 613
BseSI GKGCMC 2 cut(s) 369, 486
BseXI GCAGC 5 cut(s) 63, 214, 411, 682, 736
BshFI GGCC 2 cut(s) 329, 367
BshNI GGYRCC 1 cut(s) 483
BsiSI CCGG 3 cut(s) 192, 303, 534
BsiWI CGTACG 1 cut(s) 743
BsmAI GTCTC 1 cut(s) 257
BsnI GGCC 2 cut(s) 329, 367
Bsp120I GGGCCC 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 369, 486
Bsp143I GATC 4 cut(s) 81, 88, 454, 772
Bsp19I CCATGG 1 cut(s) 602
BspACI CCGC 8 cut(s) 180, 294, 339, 349, 380, 383, 386, 460
BspANI GGCC 2 cut(s) 329, 367
BspLI GGNNCC 3 cut(s) 328, 367, 485
BspMAI CTGCAG 1 cut(s) 726
BspOI GCTAGC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 2 cut(s) 330, 602
BssMI GATC 4 cut(s) 81, 88, 454, 772
BssT1I CCWWGG 1 cut(s) 602
Bst4CI ACNGT 4 cut(s) 135, 268, 318, 445
Bst6I CTCTTC 1 cut(s) 477
BstC8I GCNNGC 4 cut(s) 153, 178, 304, 693
BstDEI CTNAG 2 cut(s) 121, 430
BstDSI CCRYGG 1 cut(s) 602
BstF5I GGATG 4 cut(s) 91, 501, 580, 613
BstHHI GCGC 1 cut(s) 705
BstKTI GATC 4 cut(s) 84, 91, 457, 775
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 4 cut(s) 81, 88, 454, 772
BstMWI GCNNNNNNNGC 5 cut(s) 236, 299, 408, 417, 426
BstSCI CCNGG 1 cut(s) 533
BstSFI CTRYAG 2 cut(s) 518, 722
BstSLI GKGCMC 2 cut(s) 369, 486
BstV1I GCAGC 5 cut(s) 63, 214, 411, 682, 736
BsuI GTATCC 1 cut(s) 787
BsuRI GGCC 2 cut(s) 329, 367
BtgI CCRYGG 1 cut(s) 602
BtsCI GGATG 4 cut(s) 91, 501, 580, 613
Cac8I GCNNGC 4 cut(s) 153, 178, 304, 693
CfoI GCGC 1 cut(s) 705
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 4 cut(s) 327, 365, 366, 441
Csp6I GTAC 3 cut(s) 136, 146, 744
CviAII CATG 1 cut(s) 603
CviQI GTAC 3 cut(s) 136, 146, 744
DdeI CTNAG 2 cut(s) 121, 430
DpnI GATC 4 cut(s) 83, 90, 456, 774
DpnII GATC 4 cut(s) 81, 88, 454, 772
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
EciI GGCGGA 2 cut(s) 195, 354
Eco130I CCWWGG 1 cut(s) 602
Eco24I GRGCYC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 441
EcoO109I RGGNCCY 1 cut(s) 365
EcoT14I CCWWGG 1 cut(s) 602
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 602
FaeI CATG 1 cut(s) 606
FaiI YATR 9 cut(s) 7, 129, 506, 525, 548, 550, 598, 604, 755
FatI CATG 1 cut(s) 602
FauI CCCGC 1 cut(s) 379
FbaI TGATCA 2 cut(s) 454, 772
Fnu4HI GCNGC 8 cut(s) 77, 228, 294, 381, 384, 400, 696, 725
FokI GGATG 4 cut(s) 78, 508, 587, 620
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 8 cut(s) 77, 228, 294, 381, 384, 400, 696, 725
FspBI CTAG 3 cut(s) 152, 159, 438
FspI TGCGCA 1 cut(s) 704
GlaI GCGC 1 cut(s) 704
GluI GCNGC 8 cut(s) 77, 228, 294, 381, 384, 400, 696, 725
HaeIII GGCC 2 cut(s) 329, 367
HapII CCGG 3 cut(s) 192, 303, 534
HhaI GCGC 1 cut(s) 705
Hin1II CATG 1 cut(s) 606
Hin6I GCGC 1 cut(s) 703
HinP1I GCGC 1 cut(s) 703
HinfI GANTC 4 cut(s) 188, 434, 611, 649
HpaII CCGG 3 cut(s) 192, 303, 534
HphI GGTGA 2 cut(s) 449, 464
Hpy188III TCNNGA 5 cut(s) 95, 493, 653, 680, 810
Hpy99I CGWCG 7 cut(s) 44, 47, 119, 200, 203, 263, 379
HpyAV CCTTC 4 cut(s) 27, 329, 565, 742
HpyCH4III ACNGT 4 cut(s) 135, 268, 318, 445
HpyCH4IV ACGT 2 cut(s) 374, 742
HpyCH4V TGCA 4 cut(s) 504, 552, 564, 724
HpyF10VI GCNNNNNNNGC 5 cut(s) 236, 299, 408, 417, 426
HpyF3I CTNAG 2 cut(s) 121, 430
HpySE526I ACGT 2 cut(s) 374, 742
Hsp92II CATG 1 cut(s) 606
HspAI GCGC 1 cut(s) 703
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 2 cut(s) 454, 772
Kzo9I GATC 4 cut(s) 81, 88, 454, 772
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 8 cut(s) 44, 84, 108, 162, 205, 306, 316, 547
Lsp1109I GCAGC 5 cut(s) 63, 214, 411, 682, 736
LweI GCATC 1 cut(s) 471
MaeI CTAG 3 cut(s) 152, 159, 438
MaeII ACGT 2 cut(s) 374, 742
MaeIII GTNAC 1 cut(s) 35
MalI GATC 4 cut(s) 83, 90, 456, 774
MboI GATC 4 cut(s) 81, 88, 454, 772
MboII GAAGA 3 cut(s) 366, 464, 770
MfeI CAATTG 1 cut(s) 297
MhlI GDGCHC 2 cut(s) 369, 486
MluCI AATT 7 cut(s) 30, 234, 297, 393, 629, 764, 805
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 5 cut(s) 166, 228, 325, 356, 442
MroNI GCCGGC 1 cut(s) 302
MroXI GAANNNNTTC 2 cut(s) 187, 587
MseI TTAA 3 cut(s) 12, 285, 729
MslI CAYNNNNRTG 1 cut(s) 601
MspI CCGG 3 cut(s) 192, 303, 534
MspR9I CCNGG 1 cut(s) 535
MunI CAATTG 1 cut(s) 297
MwoI GCNNNNNNNGC 5 cut(s) 236, 299, 408, 417, 426
NaeI GCCGGC 1 cut(s) 304
NciI CCSGG 1 cut(s) 535
NcoI CCATGG 1 cut(s) 602
NdeII GATC 4 cut(s) 81, 88, 454, 772
NgoMIV GCCGGC 1 cut(s) 302
NheI GCTAGC 1 cut(s) 151
NlaIII CATG 1 cut(s) 606
NlaIV GGNNCC 3 cut(s) 328, 367, 485
NmeAIII GCCGAG 1 cut(s) 355
NmuCI GTSAC 1 cut(s) 35
NsbI TGCGCA 1 cut(s) 704
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PdiI GCCGGC 1 cut(s) 304
PdmI GAANNNNTTC 2 cut(s) 187, 587
PfeI GAWTC 4 cut(s) 188, 434, 611, 649
Pfl23II CGTACG 1 cut(s) 743
PkrI GCNGC 8 cut(s) 78, 229, 295, 382, 385, 401, 697, 726
PspLI CGTACG 1 cut(s) 743
PspN4I GGNNCC 3 cut(s) 328, 367, 485
PspOMI GGGCCC 1 cut(s) 365
PspPI GGNCC 4 cut(s) 327, 365, 366, 441
PstI CTGCAG 1 cut(s) 726
RsaI GTAC 3 cut(s) 137, 147, 745
RsaNI GTAC 3 cut(s) 136, 146, 744
RseI CAYNNNNRTG 1 cut(s) 601
SaqAI TTAA 3 cut(s) 12, 285, 729
SatI GCNGC 8 cut(s) 77, 228, 294, 381, 384, 400, 696, 725
Sau3AI GATC 4 cut(s) 81, 88, 454, 772
Sau96I GGNCC 4 cut(s) 327, 365, 366, 441
ScrFI CCNGG 1 cut(s) 535
SduI GDGCHC 2 cut(s) 369, 486
SfaNI GCATC 1 cut(s) 471
SfcI CTRYAG 2 cut(s) 518, 722
SinI GGWCC 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 2 cut(s) 659, 680
SmoI CTYRAG 2 cut(s) 659, 680
Sse9I AATT 7 cut(s) 30, 234, 297, 393, 629, 764, 805
SsiI CCGC 8 cut(s) 180, 294, 339, 349, 380, 383, 386, 460
SspMI CTAG 3 cut(s) 152, 159, 438
StyD4I CCNGG 1 cut(s) 533
StyI CCWWGG 1 cut(s) 602
TaaI ACNGT 4 cut(s) 135, 268, 318, 445
TaiI ACGT 2 cut(s) 377, 745
TaqI TCGA 1 cut(s) 27
TasI AATT 7 cut(s) 30, 234, 297, 393, 629, 764, 805
TauI GCSGC 3 cut(s) 296, 383, 386
TfiI GAWTC 4 cut(s) 188, 434, 611, 649
Tru1I TTAA 3 cut(s) 12, 285, 729
Tru9I TTAA 3 cut(s) 12, 285, 729
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 5 cut(s) 76, 227, 399, 695, 724
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 3 cut(s) 144, 642, 750
TspGWI ACGGA 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 441
XapI RAATTY 2 cut(s) 764, 805
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XmnI GAANNNNTTC 2 cut(s) 187, 587
XspI CTAG 3 cut(s) 152, 159, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.