pycom12g13830

UPF0161 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015936
Physical Location & Seq
Reverse (-)
16306575 .. 16307536
962 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 456 bp
ATGGCGGTCGTTATGTTGGTTAACTACCAGAAACCTCTGGCTATAGCTGTTCATCCCCAATCCCAAAACCCTAGCTTCAATTCCGGCAAAATTTACCCCATTCAATACAATTCTTGTACGGCTCGTCGTCGCCCAATAATCCATGGAGCCAATGGAGACTCGCAGTCCGACCCAAACAATCCACAAGATAAGGAAGTGGACAGTTTGGGGGTTAAAGCTGCATTATCTCTACTCAAATTCTACAGAAGGGAAATCTCACCTCTGATGTTGAAGAGTTGCCGTTATGTACCAACCTGCAGTGAGTACTCGATGCAAGCTTACAAGAAGTATGGATTTGCAAAAGGTTCCATCTTGACTGCTTGGCGAATCTGTCGTTGTAATCCTCTTGGTGGGACTGGATATGATCCTCCTCGATGGTTCGACGAGCCAAGGTTGCCTGAAGAACTTGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Haemolytic

Protein Analysis

152

Amino Acids

17.07

Weight (kDa)

8.98

Isoelectric Point (pI)

57.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YidD PF01809 73 - 136 9.6e-27 Putative membrane protein insertion efficiency factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015936)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09310
fragaria_vesca FvH4_6g13510 FvH4_6g13510
malus_domestica MD12G1143600.v1.1
prunus_persica Prupe.6G257100_v2.0.a1
pyrus_communis pycom12g13830
rosa_chinensis RchiOBHm_Chr3g0466381
rosa_laevigata RLG00000024579
rosa_multiflora Rmu_sc0003480.1_g000004
rosa_roxburghii Rroxscaffold_6G00414520
rosa_rugosa Rorug03G0081400
rosa_samantha Rh3AG127900 Rh3BG149200 Rh3DG149900
rosa_wichuraiana Rw3G012080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 302
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 398
AcsI RAATTY 2 cut(s) 90, 236
AfaI GTAC 3 cut(s) 118, 288, 305
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 3 cut(s) 79, 104, 271
AhdI GACNNNNNGTC 1 cut(s) 163
AluBI AGCT 4 cut(s) 47, 75, 218, 317
AluI AGCT 4 cut(s) 47, 75, 218, 317
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 1 cut(s) 398
ApeKI GCWGC 1 cut(s) 218
ApoI RAATTY 2 cut(s) 90, 236
AsuHPI GGTGA 1 cut(s) 249
BbvI GCAGC 1 cut(s) 205
BccI CCATC 2 cut(s) 356, 408
BceAI ACGGC 2 cut(s) 135, 264
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 3 cut(s) 42, 241, 295
BfuAI ACCTGC 1 cut(s) 302
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
BmcAI AGTACT 1 cut(s) 305
BmeRI GACNNNNNGTC 1 cut(s) 163
BmiI GGNNCC 2 cut(s) 148, 346
BmsI GCATC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 142, 428
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 400
BseDI CCNNGG 2 cut(s) 142, 428
BseGI GGATG 1 cut(s) 52
BseLI CCNNNNNNNGG 1 cut(s) 389
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 1 cut(s) 205
Bsh1285I CGRYCG 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 406
Bsp143I GATC 1 cut(s) 403
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 1 cut(s) 5
BspLI GGNNCC 2 cut(s) 148, 346
BspMAI CTGCAG 1 cut(s) 299
BspMI ACCTGC 1 cut(s) 302
BspPI GGATC 1 cut(s) 398
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 2 cut(s) 142, 428
BssMI GATC 1 cut(s) 403
BssT1I CCWWGG 2 cut(s) 142, 428
Bst4CI ACNGT 1 cut(s) 203
Bst6I CTCTTC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 315
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 52
BstKTI GATC 1 cut(s) 406
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 1 cut(s) 403
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 433
BstSFI CTRYAG 3 cut(s) 42, 241, 295
BstV1I GCAGC 1 cut(s) 205
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 1 cut(s) 52
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 304
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 315
Csp6I GTAC 3 cut(s) 117, 287, 304
CviAII CATG 1 cut(s) 143
CviJI RGCY 8 cut(s) 41, 47, 75, 122, 149, 218, 317, 427
CviKI_1 RGCY 8 cut(s) 41, 47, 75, 122, 149, 218, 317, 427
CviQI GTAC 3 cut(s) 117, 287, 304
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
DriI GACNNNNNGTC 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 266
Eam1105I GACNNNNNGTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 266
Eco130I CCWWGG 2 cut(s) 142, 428
EcoT14I CCWWGG 2 cut(s) 142, 428
ErhI CCWWGG 2 cut(s) 142, 428
FaeI CATG 1 cut(s) 146
FaiI YATR 6 cut(s) 14, 44, 144, 285, 330, 402
FaqI GGGAC 1 cut(s) 406
FatI CATG 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 1 cut(s) 39
Fsp4HI GCNGC 1 cut(s) 219
FspBI CTAG 1 cut(s) 72
GluI GCNGC 1 cut(s) 219
HapII CCGG 1 cut(s) 84
Hin1II CATG 1 cut(s) 146
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 2 cut(s) 158, 366
HpaI GTTAAC 1 cut(s) 22
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 249
Hpy166II GTNNAC 2 cut(s) 22, 199
Hpy188I TCNGA 2 cut(s) 169, 264
Hpy188III TCNNGA 1 cut(s) 352
Hpy8I GTNNAC 2 cut(s) 22, 199
Hpy99I CGWCG 3 cut(s) 129, 132, 425
HpyAV CCTTC 1 cut(s) 240
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4V TGCA 4 cut(s) 221, 297, 313, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 433
Hsp92II CATG 1 cut(s) 146
KspAI GTTAAC 1 cut(s) 22
Kzo9I GATC 1 cut(s) 403
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 6 cut(s) 23, 41, 97, 307, 381, 450
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 1 cut(s) 300
MaeI CTAG 1 cut(s) 72
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 2 cut(s) 283, 452
MluCI AATT 4 cut(s) 79, 90, 109, 236
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 5 cut(s) 45, 270, 393, 417, 420
MseI TTAA 2 cut(s) 21, 213
MspI CCGG 1 cut(s) 84
MwoI GCNNNNNNNGC 1 cut(s) 433
NcoI CCATGG 1 cut(s) 142
NdeII GATC 1 cut(s) 403
NlaIII CATG 1 cut(s) 146
NlaIV GGNNCC 2 cut(s) 148, 346
PfeI GAWTC 1 cut(s) 366
PkrI GCNGC 1 cut(s) 220
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PspN4I GGNNCC 2 cut(s) 148, 346
PstI CTGCAG 1 cut(s) 299
RsaI GTAC 3 cut(s) 118, 288, 305
RsaNI GTAC 3 cut(s) 117, 287, 304
SaqAI TTAA 2 cut(s) 21, 213
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 1 cut(s) 403
ScaI AGTACT 1 cut(s) 305
SchI GAGTC 1 cut(s) 152
SetI ASST 9 cut(s) 37, 49, 77, 220, 262, 296, 319, 346, 434
SfaNI GCATC 1 cut(s) 300
SfcI CTRYAG 3 cut(s) 42, 241, 295
Sse9I AATT 4 cut(s) 79, 90, 109, 236
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 72
StyI CCWWGG 2 cut(s) 142, 428
TaaI ACNGT 1 cut(s) 203
TaqI TCGA 3 cut(s) 308, 412, 420
TasI AATT 4 cut(s) 79, 90, 109, 236
TatI WGTACW 1 cut(s) 303
TfiI GAWTC 1 cut(s) 366
Tru1I TTAA 2 cut(s) 21, 213
Tru9I TTAA 2 cut(s) 21, 213
TscAI CASTG 1 cut(s) 304
TseI GCWGC 1 cut(s) 218
TspDTI ATGAA 1 cut(s) 41
TspRI CASTG 1 cut(s) 304
XapI RAATTY 2 cut(s) 90, 236
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XspI CTAG 1 cut(s) 72
ZrmI AGTACT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.