pycom12g18890

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009169
Physical Location & Seq
Forward (+)
20928388 .. 20929641
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18890.3

Sequence Viewer

Length: 567 bp
ATGGAATCTGGGAGGCCGGAGGAACTTCAACTTTTATTTATCTGCCACCAAAAGTCCCACAAACCTATAAATACTTGGAATTTGGAAACCTCGAATTGGATAGGAAAAAATACAGGGAGAGTTAGAAACTTCAAGCTTGGAATGCCGATGGGGAAGTATTTTTGCGATTACTGCGAGAAGGAATTCCAGGACACCCCACCTTCGAGAAGACGCCATCTTCAAAGCGGCCAACACCTTCGAGCCAGAGCTCAATGGTACGCCTCCTTCAAAGATCCCAACCATGCTTACCCGACAAAAGGGGTCTGCAACCGCTTTGTCAATACGGGATCTTGCCCATATGGTGATTCTTGTAAATACCTTCATCCCAAGAACAATCAGCAGGATACAGTTTTGATTGATAATAATCAGTCTTCGAGTTTTCAAGAGAATCAGTCAGTTGGAGGCAGCTCTTTGCCAGATGTGGTTGTCAGAGATCATACGGGAACGTCATGGAGCAATCTACCTCCATCCCTAATGCCTCCTCCAGAGGGTGGACATCCGCGGCTTCCTTTCGTAGACTGGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005689 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCCH zf-U1

Protein Analysis

189

Amino Acids

21.42

Weight (kDa)

8.75

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 530
AccI GTMKAC 1 cut(s) 555
AccII CGCG 1 cut(s) 541
AciI CCGC 4 cut(s) 225, 310, 539, 541
AclWI GGATC 2 cut(s) 266, 334
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 2 cut(s) 79, 182
AcyI GRCGYC 1 cut(s) 211
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 4 cut(s) 96, 296, 527, 530
AgsI TTSAA 5 cut(s) 29, 133, 221, 268, 422
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 3 cut(s) 136, 248, 447
AluI AGCT 3 cut(s) 136, 248, 447
Alw21I GWGCWC 1 cut(s) 250
AlwI GGATC 2 cut(s) 266, 334
AoxI GGCC 2 cut(s) 14, 226
ApeKI GCWGC 1 cut(s) 444
ApoI RAATTY 2 cut(s) 79, 182
Asp700I GAANNNNTTC 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 353
BanII GRGCYC 1 cut(s) 250
BarI GAAGNNNNNNTAC 2 cut(s) 248, 280
BbsI GAAGAC 2 cut(s) 214, 402
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 456
BccI CCATC 3 cut(s) 142, 222, 514
BciT130I CCWGG 1 cut(s) 188
BciVI GTATCC 1 cut(s) 376
BfuI GTATCC 1 cut(s) 376
BisI GCNGC 3 cut(s) 226, 445, 542
BlsI GCNGC 3 cut(s) 227, 446, 543
Bme1390I CCNGG 1 cut(s) 188
BmrFI CCNGG 1 cut(s) 188
BpiI GAAGAC 2 cut(s) 214, 402
BpmI CTGGAG 1 cut(s) 507
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 211
BsaJI CCNNGG 1 cut(s) 539
Bsc4I CCNNNNNNNGG 4 cut(s) 96, 296, 527, 530
Bse1I ACTGG 1 cut(s) 563
Bse8I GATNNNNATC 1 cut(s) 402
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 539
BseGI GGATG 3 cut(s) 361, 506, 535
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 4 cut(s) 96, 296, 527, 530
BseNI ACTGG 1 cut(s) 563
BseRI GAGGAG 1 cut(s) 510
BseXI GCAGC 1 cut(s) 456
Bsh1236I CGCG 1 cut(s) 541
BshFI GGCC 2 cut(s) 16, 228
BsiHKAI GWGCWC 1 cut(s) 250
BsiSI CCGG 1 cut(s) 17
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 4 cut(s) 96, 296, 527, 530
BsmFI GGGAC 1 cut(s) 40
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 2 cut(s) 16, 228
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 3 cut(s) 271, 326, 472
BspACI CCGC 4 cut(s) 225, 310, 539, 541
BspANI GGCC 2 cut(s) 16, 228
BspFNI CGCG 1 cut(s) 541
BspPI GGATC 2 cut(s) 266, 334
BsrI ACTGG 1 cut(s) 563
BssECI CCNNGG 1 cut(s) 539
BssMI GATC 3 cut(s) 271, 326, 472
BssNI GRCGYC 1 cut(s) 211
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 388
BstACI GRCGYC 1 cut(s) 211
BstDSI CCRYGG 1 cut(s) 539
BstF5I GGATG 3 cut(s) 361, 506, 535
BstFNI CGCG 1 cut(s) 541
BstKTI GATC 3 cut(s) 274, 329, 475
BstMBI GATC 3 cut(s) 271, 326, 472
BstMWI GCNNNNNNNGC 2 cut(s) 142, 171
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstUI CGCG 1 cut(s) 541
BstV1I GCAGC 1 cut(s) 456
BstV2I GAAGAC 2 cut(s) 214, 402
BstX2I RGATCY 2 cut(s) 271, 326
BstYI RGATCY 2 cut(s) 271, 326
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 2 cut(s) 16, 228
BtgI CCRYGG 1 cut(s) 539
BtsCI GGATG 3 cut(s) 361, 506, 535
Cfr42I CCGCGG 1 cut(s) 542
CseI GACGC 1 cut(s) 219
Csp6I GTAC 1 cut(s) 256
CviAII CATG 2 cut(s) 281, 489
CviJI RGCY 7 cut(s) 16, 136, 228, 242, 248, 447, 544
CviKI_1 RGCY 7 cut(s) 16, 136, 228, 242, 248, 447, 544
CviQI GTAC 1 cut(s) 256
DpnI GATC 3 cut(s) 273, 328, 474
DpnII GATC 3 cut(s) 271, 326, 472
EaeI YGGCCR 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 248
Eco24I GRGCYC 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 248
EcoRI GAATTC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 250
FaeI CATG 2 cut(s) 284, 492
FaiI YATR 6 cut(s) 68, 282, 337, 339, 477, 490
FaqI GGGAC 1 cut(s) 40
FatI CATG 2 cut(s) 280, 488
FauNDI CATATG 1 cut(s) 337
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 3 cut(s) 226, 445, 542
FokI GGATG 3 cut(s) 348, 493, 522
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 3 cut(s) 226, 445, 542
GluI GCNGC 3 cut(s) 226, 445, 542
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 2 cut(s) 16, 228
HapII CCGG 1 cut(s) 17
HgaI GACGC 1 cut(s) 219
Hin1I GRCGYC 1 cut(s) 211
Hin1II CATG 2 cut(s) 284, 492
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 3 cut(s) 5, 344, 427
HpaII CCGG 1 cut(s) 17
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 2 cut(s) 533, 556
Hpy188I TCNGA 1 cut(s) 470
Hpy188III TCNNGA 3 cut(s) 204, 422, 524
Hpy8I GTNNAC 2 cut(s) 533, 556
HpyAV CCTTC 5 cut(s) 172, 210, 245, 274, 368
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 1 cut(s) 306
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 171
HpySE526I ACGT 1 cut(s) 485
Hsp92I GRCGYC 1 cut(s) 211
Hsp92II CATG 2 cut(s) 284, 492
KspI CCGCGG 1 cut(s) 542
Kzo9I GATC 3 cut(s) 271, 326, 472
LmnI GCTCC 1 cut(s) 492
LpnPI CCDG 9 cut(s) 30, 99, 173, 200, 256, 365, 468, 537, 544
Lsp1109I GCAGC 1 cut(s) 456
MaeII ACGT 1 cut(s) 485
MalI GATC 3 cut(s) 273, 328, 474
MboI GATC 3 cut(s) 271, 326, 472
MboII GAAGA 3 cut(s) 209, 219, 402
MflI RGATCY 2 cut(s) 271, 326
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 3 cut(s) 79, 94, 182
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 9 cut(s) 6, 13, 100, 271, 434, 513, 520, 528, 531
MroXI GAANNNNTTC 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 541
MspI CCGG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 188
Mva1269I GAATGC 1 cut(s) 147
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 541
MwoI GCNNNNNNNGC 2 cut(s) 142, 171
NdeI CATATG 1 cut(s) 337
NdeII GATC 3 cut(s) 271, 326, 472
NlaIII CATG 2 cut(s) 284, 492
PctI GAATGC 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 3 cut(s) 5, 344, 427
PflMI CCANNNNNTGG 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 3 cut(s) 227, 446, 543
Psp124BI GAGCTC 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PsuI RGATCY 2 cut(s) 271, 326
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SacI GAGCTC 1 cut(s) 250
SacII CCGCGG 1 cut(s) 542
SatI GCNGC 3 cut(s) 226, 445, 542
Sau3AI GATC 3 cut(s) 271, 326, 472
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 250
Sfr303I CCGCGG 1 cut(s) 542
SgrBI CCGCGG 1 cut(s) 542
Sse9I AATT 3 cut(s) 79, 94, 182
SsiI CCGC 4 cut(s) 225, 310, 539, 541
SstI GAGCTC 1 cut(s) 250
StyD4I CCNGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 1 cut(s) 488
TaqI TCGA 4 cut(s) 92, 203, 238, 413
TasI AATT 3 cut(s) 79, 94, 182
TauI GCSGC 2 cut(s) 228, 544
TfiI GAWTC 3 cut(s) 5, 344, 427
TseI GCWGC 1 cut(s) 444
TspDTI ATGAA 1 cut(s) 350
Van91I CCANNNNNTGG 1 cut(s) 530
XapI RAATTY 2 cut(s) 79, 182
XmiI GTMKAC 1 cut(s) 555
XmnI GAANNNNTTC 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.