pycom12g19570

Calmodulin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007733
Physical Location & Seq
Forward (+)
21368573 .. 21371494
2922 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1743 bp
ATGGTCACCAAGAGGCATTTTCGCGACCGGGGAAGCGAGGACTTCGAGTTTCCTGTTCCAGAATCCAAAAGGAGGCCTACTTTTAGGAATGTTGTTAGGGATGTGATGAGAGACGCCACGCTGAATGAACGCGAGTGGGAAAGCTTCTTACGGAGAATTGTGCGAGAGGAGGTGGAGCGCGGTATTCTTCCCTTCCTCATGTCATCTCCAAGGCCGTCGCTGGAAAGTGGGAGTACTTCTGGAGGAAGTGGCTTGCAGTTGCGTTTTGTCAACAAGCTGGCAGGAACCATATTCACAGGCGGTAGGGTGGAAGCAGAGAATGGCGAGCCTCTTCAAATTGAACTAGTCGATGCCAGTACCGGTGCTATAGTCCGTTCCGGTCCCCTATCTTTGCTGAAGCTTGAACTTCTTGTGGTGAATGGTGAGTTCGGGTCTGATGATCAAGAGGATTGGACCGAACGAGAATTCAACAACTACATTGTTCGCGCAAGGGATGGCAAGAGGCCATTAGTGACGGGCGAGATACATGTTACACTAAGAGAGGGGGCTGGTTCTGTTGGTGATGTTATGTTTACTGACAACTCGAGCTGGATAAGAAGCCGAACGTTCAGACTAGCAGCTCGAGCTGTGGCCAAATCTTCGAGTGAAGTACGAATTAGAGAAGCTAGAAGTGAACCGTTTGTGGTTAAAGATCACCGTGGAGAATTGTATAAGAAGCACTACCCTCCAAACCTAAACGACGAAATATGGCGCCTGGAAAAGATAGCGAAAGACGGAGCCTTCCATAAGAGACTGTGCGAGAATGGAATCAGCACAGTGGAGGAGTTCCTGCAGTTGTACATGAGAGATGTATCCTCACTACGAAATGCTTTCGGTGGGATCTCGACCAAGATATGGGAAACAATCATAGAGCATGCTATGACCTGTAAGTTGGATGACCATAAGTGCTATGCCTATCATAGAGCTGAGCACGATGTAAGTCTCATGTTCAATTCCATCCACAGGCTTGAAGGGGCAATAATCAATGGCCAGTTCTGTTCTCTGGATGAACTCGACGCGCATCAGAAGATGCTGGTGGAAACTCTGAAGCAGCATGCTTATCAAAATGTAAGAGATTTGATCCCTGTCGATTCTTCAACTATATTTGGCCTTTCGAGGCCCTTGCCATGTCCACAAGCTGAGCAATTCACCAGTCCAAACCCTGATCTGCAACAAATTCAATTTCAGTTCACACATCAAGATGAACTCCCAATACAACTAGGTTTCAACCATGGATCAGCTTCAACCTCATGTGCCTACCAAGCAGAAGGCAGCAGCAATCAGTTAATGGTTTCTCTAGCACAAAGCCAACCAATGCAACTCCGAAGCAACAGCTTTTCGATGGAGGACTTTAACTCCGTCATCTACAATGAAGAAAGCAGTTGGCCGCCTCTCGGTGTTTTCCAAGCGCCAAATCTCCCAACCGGTCATTTAGGGACAGAAAACCTTTTCCAGGTCCAAGCATCAACTTGGTCTCCTGCAAACCCAATATGGGGACAAACAGGCGGCTTCTACTTCACTTCAGGTAGCGAAGCAGAAGCCGCACATTTCCCTCTTCCCGCTTTTGTACATAACGCAGGGACTAGACGGAAACCCAAGGCATGCTGGTGCAAGCTTCGTGCCGCCATTAAATGGTGGGTCTCCGTTAGGAAGCATGGGAGTGCTAGAAGAATGGCAAGGCCTCCTATGTATCTGAACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0002229 GO:0002237 GO:0002239 GO:0002376 GO:0002682 GO:0002831 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006725 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009696 GO:0009697 GO:0009787 GO:0009789 GO:0009814 GO:0009816 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010112 GO:0010224 GO:0010337 GO:0010468 GO:0010556 GO:0010565 GO:0010646 GO:0010647 GO:0010817 GO:0016053 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0023051 GO:0023056 GO:0031323 GO:0031326 GO:0031347 GO:0032101 GO:0032350 GO:0032787 GO:0033554 GO:0036293 GO:0036294 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0043900 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0046189 GO:0046394 GO:0046885 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070482 GO:0070887 GO:0071216 GO:0071219 GO:0071453 GO:0071456 GO:0071704 GO:0072330 GO:0080090 GO:0080134 GO:0080142 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901419 GO:1901421 GO:1901576 GO:1901615 GO:1901617 GO:1902584 GO:1903506 GO:1905957 GO:1905959 GO:2000070 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

581

Amino Acids

65.67

Weight (kDa)

6.83

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 85 - 232 1.1e-57 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 245 - 309 2.4e-28 Calmodulin binding protein central domain
Calmod_bind_C PF20452 315 - 375 2.1e-18 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 750
AccB7I CCANNNNNTGG 2 cut(s) 894, 1671
AccII CGCG 5 cut(s) 24, 132, 180, 486, 1058
AciI CCGC 7 cut(s) 180, 300, 1427, 1545, 1581, 1599, 1662
AclI AACGTT 1 cut(s) 605
AclWI GGATC 3 cut(s) 887, 1114, 1282
AcoI YGGCCR 3 cut(s) 630, 1027, 1424
AcsI RAATTY 2 cut(s) 464, 1215
AcuI CTGAAG 3 cut(s) 416, 1106, 1545
AcyI GRCGYC 2 cut(s) 114, 751
AfaI GTAC 5 cut(s) 235, 358, 651, 839, 1608
AfiI CCNNNNNNNGG 8 cut(s) 72, 894, 1002, 1433, 1492, 1531, 1532, 1671
AflIII ACRYGT 1 cut(s) 526
AgeI ACCGGT 2 cut(s) 359, 1463
AhlI ACTAGT 1 cut(s) 343
AjnI CCWGG 2 cut(s) 753, 1491
Alw21I GWGCWC 1 cut(s) 972
Alw26I GTCTC 5 cut(s) 105, 784, 986, 1518, 1684
AlwI GGATC 3 cut(s) 887, 1114, 1282
Ama87I CYCGRG 2 cut(s) 583, 621
AoxI GGCC 9 cut(s) 74, 212, 503, 630, 1027, 1147, 1157, 1424, 1718
ApeKI GCWGC 4 cut(s) 617, 1090, 1311, 1314
ApoI RAATTY 2 cut(s) 464, 1215
AsiGI ACCGGT 2 cut(s) 359, 1463
AspLEI GCGC 5 cut(s) 180, 488, 753, 1060, 1450
AspS9I GGNCC 4 cut(s) 380, 453, 1158, 1495
AsuC2I CCSGG 1 cut(s) 29
AsuHPI GGTGA 5 cut(s) 427, 434, 572, 686, 1180
AvaI CYCGRG 2 cut(s) 583, 621
AvaII GGWCC 3 cut(s) 380, 453, 1495
BalI TGGCCA 2 cut(s) 632, 1029
BanI GGYRCC 1 cut(s) 750
Bbv12I GWGCWC 1 cut(s) 972
BbvI GCAGC 4 cut(s) 629, 1102, 1323, 1326
BccI CCATC 3 cut(s) 488, 1004, 1375
BceAI ACGGC 1 cut(s) 199
BcgI CGANNNNNNTGC 2 cut(s) 1144, 1178
BciT130I CCWGG 2 cut(s) 755, 1493
BciVI GTATCC 1 cut(s) 862
BclI TGATCA 1 cut(s) 439
BcnI CCSGG 1 cut(s) 29
BcoDI GTCTC 5 cut(s) 105, 784, 986, 1518, 1684
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 8 cut(s) 344, 614, 666, 1259, 1337, 1623, 1704, 1741
BfmI CTRYAG 2 cut(s) 366, 830
BfoI RGCGCY 2 cut(s) 754, 1451
BfuI GTATCC 1 cut(s) 862
BisI GCNGC 8 cut(s) 618, 1091, 1312, 1315, 1427, 1546, 1581, 1662
BlpI GCTNAGC 2 cut(s) 966, 1179
BlsI GCNGC 8 cut(s) 619, 1092, 1313, 1316, 1428, 1547, 1582, 1663
BmcAI AGTACT 1 cut(s) 235
Bme1390I CCNGG 3 cut(s) 29, 755, 1493
Bme18I GGWCC 3 cut(s) 380, 453, 1495
BmeT110I CYCGRG 2 cut(s) 583, 621
BmgT120I GGNCC 4 cut(s) 380, 453, 1158, 1495
BmiI GGNNCC 4 cut(s) 286, 382, 752, 778
BmrFI CCNGG 3 cut(s) 29, 755, 1493
BmsI GCATC 4 cut(s) 340, 1059, 1069, 1511
BpmI CTGGAG 1 cut(s) 261
Bpu1102I GCTNAGC 2 cut(s) 966, 1179
BpuMI CCSGG 1 cut(s) 29
BsaHI GRCGYC 2 cut(s) 114, 751
BsaI GGTCTC 2 cut(s) 1518, 1684
BsaJI CCNNGG 5 cut(s) 28, 209, 697, 1270, 1635
BsaWI WCCGGW 3 cut(s) 359, 377, 1463
BsaXI ACNNNNNCTCC 4 cut(s) 1379, 1409, 1498, 1528
Bsc4I CCNNNNNNNGG 8 cut(s) 72, 894, 1002, 1433, 1492, 1531, 1532, 1671
Bse118I RCCGGY 2 cut(s) 359, 1463
Bse1I ACTGG 3 cut(s) 354, 1030, 1191
BseBI CCWGG 2 cut(s) 755, 1493
BseDI CCNNGG 5 cut(s) 28, 209, 697, 1270, 1635
BseGI GGATG 5 cut(s) 106, 499, 940, 996, 1051
BseLI CCNNNNNNNGG 8 cut(s) 72, 894, 1002, 1433, 1492, 1531, 1532, 1671
BseMII CTCAG 2 cut(s) 957, 1170
BseNI ACTGG 3 cut(s) 354, 1030, 1191
BseRI GAGGAG 2 cut(s) 182, 836
BseXI GCAGC 4 cut(s) 629, 1102, 1323, 1326
Bsh1236I CGCG 5 cut(s) 24, 132, 180, 486, 1058
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 9 cut(s) 76, 214, 505, 632, 1029, 1149, 1159, 1426, 1720
BshNI GGYRCC 1 cut(s) 750
BshTI ACCGGT 2 cut(s) 359, 1463
BsiEI CGRYCG 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 972
BsiHKCI CYCGRG 2 cut(s) 583, 621
BsiSI CCGG 4 cut(s) 28, 360, 378, 1464
BslFI GGGAC 4 cut(s) 366, 1489, 1548, 1633
BslI CCNNNNNNNGG 8 cut(s) 72, 894, 1002, 1433, 1492, 1531, 1532, 1671
BsmAI GTCTC 5 cut(s) 105, 784, 986, 1518, 1684
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 4 cut(s) 366, 1489, 1548, 1633
BsnI GGCC 9 cut(s) 76, 214, 505, 632, 1029, 1149, 1159, 1426, 1720
Bso31I GGTCTC 2 cut(s) 1518, 1684
BsoBI CYCGRG 2 cut(s) 583, 621
Bsp1286I GDGCHC 1 cut(s) 972
Bsp1407I TGTACA 2 cut(s) 837, 1606
Bsp143I GATC 6 cut(s) 439, 691, 879, 1119, 1204, 1274
Bsp1720I GCTNAGC 2 cut(s) 966, 1179
Bsp19I CCATGG 1 cut(s) 1270
Bsp68I TCGCGA 1 cut(s) 24
BspACI CCGC 7 cut(s) 180, 300, 1427, 1545, 1581, 1599, 1662
BspANI GGCC 9 cut(s) 76, 214, 505, 632, 1029, 1149, 1159, 1426, 1720
BspCNI CTCAG 2 cut(s) 958, 1171
BspFNI CGCG 5 cut(s) 24, 132, 180, 486, 1058
BspLI GGNNCC 4 cut(s) 286, 382, 752, 778
BspMAI CTGCAG 1 cut(s) 834
BspPI GGATC 3 cut(s) 887, 1114, 1282
BspT107I GGYRCC 1 cut(s) 750
BspTNI GGTCTC 2 cut(s) 1518, 1684
BsrFI RCCGGY 2 cut(s) 359, 1463
BsrGI TGTACA 2 cut(s) 837, 1606
BsrI ACTGG 3 cut(s) 354, 1030, 1191
BssAI RCCGGY 2 cut(s) 359, 1463
BssECI CCNNGG 5 cut(s) 28, 209, 697, 1270, 1635
BssMI GATC 6 cut(s) 439, 691, 879, 1119, 1204, 1274
BssNI GRCGYC 2 cut(s) 114, 751
BssT1I CCWWGG 3 cut(s) 209, 1270, 1635
Bst2UI CCWGG 2 cut(s) 755, 1493
Bst4CI ACNGT 4 cut(s) 678, 698, 795, 817
Bst6I CTCTTC 2 cut(s) 336, 1599
BstACI GRCGYC 2 cut(s) 114, 751
BstAUI TGTACA 2 cut(s) 837, 1606
BstC8I GCNNGC 7 cut(s) 254, 279, 326, 915, 1095, 1642, 1652
BstDEI CTNAG 3 cut(s) 536, 966, 1179
BstDSI CCRYGG 2 cut(s) 697, 1270
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 1 cut(s) 1490
BstF5I GGATG 5 cut(s) 106, 499, 940, 996, 1051
BstFNI CGCG 5 cut(s) 24, 132, 180, 486, 1058
BstH2I RGCGCY 2 cut(s) 754, 1451
BstHHI GCGC 5 cut(s) 180, 488, 753, 1060, 1450
BstKTI GATC 6 cut(s) 442, 694, 882, 1122, 1207, 1277
BstMAI GTCTC 5 cut(s) 105, 784, 986, 1518, 1684
BstMBI GATC 6 cut(s) 439, 691, 879, 1119, 1204, 1274
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 3 cut(s) 623, 1301, 1580
BstNI CCWGG 2 cut(s) 755, 1493
BstNSI RCATGY 4 cut(s) 530, 917, 1097, 1644
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 3 cut(s) 27, 753, 1491
BstSFI CTRYAG 2 cut(s) 366, 830
BstUI CGCG 5 cut(s) 24, 132, 180, 486, 1058
BstV1I GCAGC 4 cut(s) 629, 1102, 1323, 1326
BstX2I RGATCY 1 cut(s) 879
BstYI RGATCY 1 cut(s) 879
BsuI GTATCC 1 cut(s) 862
BsuRI GGCC 9 cut(s) 76, 214, 505, 632, 1029, 1149, 1159, 1426, 1720
BtgI CCRYGG 2 cut(s) 697, 1270
BtsCI GGATG 5 cut(s) 106, 499, 940, 996, 1051
BtsIMutI CAGTG 1 cut(s) 822
BtuMI TCGCGA 1 cut(s) 24
Cac8I GCNNGC 7 cut(s) 254, 279, 326, 915, 1095, 1642, 1652
CfoI GCGC 5 cut(s) 180, 488, 753, 1060, 1450
Cfr10I RCCGGY 2 cut(s) 359, 1463
Cfr13I GGNCC 4 cut(s) 380, 453, 1158, 1495
CseI GACGC 2 cut(s) 122, 1064
Csp6I GTAC 5 cut(s) 234, 357, 650, 838, 1607
CspAI ACCGGT 2 cut(s) 359, 1463
CviQI GTAC 5 cut(s) 234, 357, 650, 838, 1607
DdeI CTNAG 3 cut(s) 536, 966, 1179
DinI GGCGCC 1 cut(s) 752
DpnI GATC 6 cut(s) 441, 693, 881, 1121, 1206, 1276
DpnII GATC 6 cut(s) 439, 691, 879, 1119, 1204, 1274
EaeI YGGCCR 3 cut(s) 630, 1027, 1424
Eam1104I CTCTTC 2 cut(s) 336, 1599
EarI CTCTTC 2 cut(s) 336, 1599
Eco130I CCWWGG 3 cut(s) 209, 1270, 1635
Eco147I AGGCCT 2 cut(s) 76, 1720
Eco31I GGTCTC 2 cut(s) 1518, 1684
Eco47I GGWCC 3 cut(s) 380, 453, 1495
Eco57I CTGAAG 3 cut(s) 416, 1106, 1545
Eco88I CYCGRG 2 cut(s) 583, 621
Eco91I GGTNACC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 1490
EcoO109I RGGNCCY 1 cut(s) 1158
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 753, 1491
EcoT14I CCWWGG 3 cut(s) 209, 1270, 1635
EgeI GGCGCC 1 cut(s) 752
EheI GGCGCC 1 cut(s) 752
ErhI CCWWGG 3 cut(s) 209, 1270, 1635
Esp3I CGTCTC 1 cut(s) 105
FaqI GGGAC 4 cut(s) 366, 1489, 1548, 1633
FauI CCCGC 1 cut(s) 1606
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 8 cut(s) 618, 1091, 1312, 1315, 1427, 1546, 1581, 1662
FokI GGATG 5 cut(s) 113, 506, 947, 983, 1058
Fsp4HI GCNGC 8 cut(s) 618, 1091, 1312, 1315, 1427, 1546, 1581, 1662
FspBI CTAG 8 cut(s) 344, 614, 666, 1259, 1337, 1623, 1704, 1741
GlaI GCGC 5 cut(s) 179, 487, 752, 1059, 1449
GluI GCNGC 8 cut(s) 618, 1091, 1312, 1315, 1427, 1546, 1581, 1662
GsuI CTGGAG 1 cut(s) 261
HaeII RGCGCY 2 cut(s) 754, 1451
HaeIII GGCC 9 cut(s) 76, 214, 505, 632, 1029, 1149, 1159, 1426, 1720
HapII CCGG 4 cut(s) 28, 360, 378, 1464
HgaI GACGC 2 cut(s) 122, 1064
HhaI GCGC 5 cut(s) 180, 488, 753, 1060, 1450
Hin1I GRCGYC 2 cut(s) 114, 751
Hin6I GCGC 5 cut(s) 178, 486, 751, 1058, 1448
HinP1I GCGC 5 cut(s) 178, 486, 751, 1058, 1448
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 3 cut(s) 142, 398, 1652
HinfI GANTC 3 cut(s) 62, 807, 1130
HpaII CCGG 4 cut(s) 28, 360, 378, 1464
HphI GGTGA 5 cut(s) 427, 434, 572, 686, 1180
Hpy166II GTNNAC 5 cut(s) 271, 573, 674, 1172, 1230
Hpy188I TCNGA 6 cut(s) 436, 611, 1065, 1086, 1364, 1734
Hpy188III TCNNGA 7 cut(s) 23, 59, 240, 443, 883, 1043, 1238
Hpy8I GTNNAC 5 cut(s) 271, 573, 674, 1172, 1230
Hpy99I CGWCG 3 cut(s) 220, 743, 1058
HpyAV CCTTC 4 cut(s) 202, 790, 1004, 1301
HpyCH4III ACNGT 4 cut(s) 678, 698, 795, 817
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 6 cut(s) 256, 832, 1210, 1357, 1520, 1650
HpyF10VI GCNNNNNNNGC 3 cut(s) 623, 1301, 1580
HpyF3I CTNAG 3 cut(s) 536, 966, 1179
HpySE526I ACGT 1 cut(s) 605
Hsp92I GRCGYC 2 cut(s) 114, 751
HspAI GCGC 5 cut(s) 178, 486, 751, 1058, 1448
KasI GGCGCC 1 cut(s) 750
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 6 cut(s) 439, 691, 879, 1119, 1204, 1274
LmnI GCTCC 2 cut(s) 175, 776
Lsp1109I GCAGC 4 cut(s) 629, 1102, 1323, 1326
LweI GCATC 4 cut(s) 340, 1059, 1069, 1511
MaeI CTAG 8 cut(s) 344, 614, 666, 1259, 1337, 1623, 1704, 1741
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 3 cut(s) 4, 511, 529
MalI GATC 6 cut(s) 441, 693, 881, 1121, 1206, 1276
MboI GATC 6 cut(s) 439, 691, 879, 1119, 1204, 1274
MboII GAAGA 8 cut(s) 179, 323, 630, 1078, 1125, 1424, 1586, 1719
MflI RGATCY 1 cut(s) 879
MhlI GDGCHC 1 cut(s) 972
MlsI TGGCCA 2 cut(s) 632, 1029
MluCI AATT 9 cut(s) 156, 336, 464, 654, 704, 991, 1184, 1215, 1220
MluNI TGGCCA 2 cut(s) 632, 1029
Mly113I GGCGCC 1 cut(s) 751
MmeI TCCRAC 1 cut(s) 912
Mox20I TGGCCA 2 cut(s) 632, 1029
MscI TGGCCA 2 cut(s) 632, 1029
MseI TTAA 4 cut(s) 687, 1325, 1392, 1668
MslI CAYNNNNRTG 3 cut(s) 1239, 1645, 1698
Msp20I TGGCCA 2 cut(s) 632, 1029
MspI CCGG 4 cut(s) 28, 360, 378, 1464
MspR9I CCNGG 3 cut(s) 29, 755, 1493
MvaI CCWGG 2 cut(s) 755, 1493
MvnI CGCG 5 cut(s) 24, 132, 180, 486, 1058
MwoI GCNNNNNNNGC 3 cut(s) 623, 1301, 1580
NarI GGCGCC 1 cut(s) 751
NciI CCSGG 1 cut(s) 29
NcoI CCATGG 1 cut(s) 1270
NdeII GATC 6 cut(s) 439, 691, 879, 1119, 1204, 1274
NlaIV GGNNCC 4 cut(s) 286, 382, 752, 778
NmuCI GTSAC 2 cut(s) 4, 511
NruI TCGCGA 1 cut(s) 24
NspI RCATGY 4 cut(s) 530, 917, 1097, 1644
PaeI GCATGC 3 cut(s) 917, 1097, 1644
PaeR7I CTCGAG 2 cut(s) 583, 621
PceI AGGCCT 2 cut(s) 76, 1720
PciI ACATGT 1 cut(s) 526
PfeI GAWTC 3 cut(s) 62, 807, 1130
PflMI CCANNNNNTGG 2 cut(s) 894, 1671
PinAI ACCGGT 2 cut(s) 359, 1463
PkrI GCNGC 8 cut(s) 619, 1092, 1313, 1316, 1428, 1547, 1582, 1663
PluTI GGCGCC 1 cut(s) 754
PscI ACATGT 1 cut(s) 526
Psp1406I AACGTT 1 cut(s) 605
Psp6I CCWGG 2 cut(s) 753, 1491
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 753, 1491
PspN4I GGNNCC 4 cut(s) 286, 382, 752, 778
PspPI GGNCC 4 cut(s) 380, 453, 1158, 1495
PspXI VCTCGAGB 2 cut(s) 583, 621
PsrI GAACNNNNNNTAC 2 cut(s) 1236, 1268
PstI CTGCAG 1 cut(s) 834
PsuI RGATCY 1 cut(s) 879
RruI TCGCGA 1 cut(s) 24
RsaI GTAC 5 cut(s) 235, 358, 651, 839, 1608
RsaNI GTAC 5 cut(s) 234, 357, 650, 838, 1607
RseI CAYNNNNRTG 3 cut(s) 1239, 1645, 1698
SaqAI TTAA 4 cut(s) 687, 1325, 1392, 1668
SatI GCNGC 8 cut(s) 618, 1091, 1312, 1315, 1427, 1546, 1581, 1662
Sau3AI GATC 6 cut(s) 439, 691, 879, 1119, 1204, 1274
Sau96I GGNCC 4 cut(s) 380, 453, 1158, 1495
ScaI AGTACT 1 cut(s) 235
ScrFI CCNGG 3 cut(s) 29, 755, 1493
SduI GDGCHC 1 cut(s) 972
SfaNI GCATC 4 cut(s) 340, 1059, 1069, 1511
SfcI CTRYAG 2 cut(s) 366, 830
SfoI GGCGCC 1 cut(s) 752
Sfr274I CTCGAG 2 cut(s) 583, 621
SinI GGWCC 3 cut(s) 380, 453, 1495
SlaI CTCGAG 2 cut(s) 583, 621
SmiMI CAYNNNNRTG 3 cut(s) 1239, 1645, 1698
SmlI CTYRAG 2 cut(s) 583, 621
SmoI CTYRAG 2 cut(s) 583, 621
SpeI ACTAGT 1 cut(s) 343
SphI GCATGC 3 cut(s) 917, 1097, 1644
Sse9I AATT 9 cut(s) 156, 336, 464, 654, 704, 991, 1184, 1215, 1220
SseBI AGGCCT 2 cut(s) 76, 1720
SsiI CCGC 7 cut(s) 180, 300, 1427, 1545, 1581, 1599, 1662
SspDI GGCGCC 1 cut(s) 750
SspMI CTAG 8 cut(s) 344, 614, 666, 1259, 1337, 1623, 1704, 1741
StuI AGGCCT 2 cut(s) 76, 1720
StyD4I CCNGG 3 cut(s) 27, 753, 1491
StyI CCWWGG 3 cut(s) 209, 1270, 1635
TaaI ACNGT 4 cut(s) 678, 698, 795, 817
TaiI ACGT 1 cut(s) 608
TaqII GACCGA 1 cut(s) 470
TasI AATT 9 cut(s) 156, 336, 464, 654, 704, 991, 1184, 1215, 1220
TatI WGTACW 3 cut(s) 233, 837, 1606
TauI GCSGC 4 cut(s) 1429, 1548, 1583, 1664
TfiI GAWTC 3 cut(s) 62, 807, 1130
Tru1I TTAA 4 cut(s) 687, 1325, 1392, 1668
Tru9I TTAA 4 cut(s) 687, 1325, 1392, 1668
TscAI CASTG 1 cut(s) 822
TseFI GTSAC 2 cut(s) 4, 511
TseI GCWGC 4 cut(s) 617, 1090, 1311, 1314
Tsp45I GTSAC 2 cut(s) 4, 511
TspDTI ATGAA 4 cut(s) 141, 1062, 1257, 1425
TspGWI ACGGA 6 cut(s) 166, 362, 789, 1387, 1642, 1672
TspRI CASTG 1 cut(s) 822
Van91I CCANNNNNTGG 2 cut(s) 894, 1671
VpaK11BI GGWCC 3 cut(s) 380, 453, 1495
XagI CCTNNNNNAGG 1 cut(s) 1490
XapI RAATTY 2 cut(s) 464, 1215
XceI RCATGY 4 cut(s) 530, 917, 1097, 1644
XhoI CTCGAG 2 cut(s) 583, 621
XspI CTAG 8 cut(s) 344, 614, 666, 1259, 1337, 1623, 1704, 1741
ZrmI AGTACT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.