pycom12g19710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017955
Physical Location & Seq
Forward (+)
21443540 .. 21444133
594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g19710.1

Sequence Viewer

Length: 594 bp
ATGCAGAAAATAATCCCACAAAACCGACACCCGTTTTCATACTTTACTAGGATCTCCATTCCCCAGCCAAAAATAAGGATCAAGGTCCCCAACCCCAAGTTGCTGCTCAAAACCATGCCGAAACGCTTTCACTATGGATGCAACATGAACAACGCTGATGGAGATAATGAGCAGCAACAGCAGTGCTGCACAAAAACGAAGACGGAGAAAACAACAAAGAAGCCCGGAAAGCTTCTGACAACGAAAATCCGTTCGGTGTTCGTCAATGCGTTCAGCAGCATGAGGATCGAGTCACCAGATGGCGGAAACTTGATGAAAAGCGAAACCAAGAGTGACAGAGTGGTAGGGGAACATGGAAGTTCCGGCAACTTGAATGGGGATCCATCGAAGTTAATGGTAACTGACGGCTGGAAGTCGAGAGCGGGAAGGTACCTGGGGAACATGTTAAGAAGGGAGTTTGATGAGGAAGATAATTCGAAGCAAGGAGAAAGAGAAGGGGAGGAGTTGTGCAAGAAGAGGATTCTAATGGGAGGCAAGTGCAAGCCACTGAGTTTATCAGAAACTCTCGAGTACGATCAAGATGGCAATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.43

Weight (kDa)

9.49

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27020
fragaria_vesca FvH4_6g07020
prunus_persica Prupe.6G317300_v2.0.a1
pyrus_communis pycom12g19710
rosa_laevigata RLG00000025295
rosa_roxburghii Rroxscaffold_6G00422640
rosa_rugosa Rorug03G0013900
rosa_samantha Rh3AG074500 Rh3BG076200 Rh3CG075900 Rh3DG078000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 429
AccB1I GGYRCC 1 cut(s) 429
AccBSI CCGCTC 1 cut(s) 422
AciI CCGC 2 cut(s) 303, 422
AclWI GGATC 5 cut(s) 59, 86, 293, 374, 387
AfaI GTAC 2 cut(s) 431, 572
AfiI CCNNNNNNNGG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 1 cut(s) 373
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 1 cut(s) 232
AluI AGCT 1 cut(s) 232
AlwI GGATC 5 cut(s) 59, 86, 293, 374, 387
Ama87I CYCGRG 1 cut(s) 566
ApeKI GCWGC 4 cut(s) 103, 172, 186, 276
Asp718I GGTACC 1 cut(s) 429
AspS9I GGNCC 1 cut(s) 85
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 285
AsuII TTCGAA 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 566
AvaII GGWCC 1 cut(s) 85
BamHI GGATCC 1 cut(s) 379
BanI GGYRCC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 206
BbvI GCAGC 4 cut(s) 90, 173, 184, 288
BccI CCATC 4 cut(s) 152, 293, 391, 575
BceAI ACGGC 1 cut(s) 421
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 1 cut(s) 434
BcnI CCSGG 1 cut(s) 225
BfaI CTAG 1 cut(s) 48
BisI GCNGC 4 cut(s) 104, 173, 187, 277
BlsI GCNGC 4 cut(s) 105, 174, 188, 278
Bme1390I CCNGG 2 cut(s) 225, 434
Bme18I GGWCC 1 cut(s) 85
BmeT110I CYCGRG 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 3 cut(s) 87, 381, 431
BmrFI CCNGG 2 cut(s) 225, 434
BmsI GCATC 1 cut(s) 128
BpiI GAAGAC 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 433
BsaXI ACNNNNNCTCC 2 cut(s) 491, 521
Bsc4I CCNNNNNNNGG 1 cut(s) 302
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMII CTCAG 1 cut(s) 539
BseRI GAGGAG 1 cut(s) 515
BseXI GCAGC 4 cut(s) 90, 173, 184, 288
BseYI CCCAGC 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 172
BshNI GGYRCC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 566
BsiSI CCGG 2 cut(s) 225, 363
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 71
BsoBI CYCGRG 1 cut(s) 566
Bsp119I TTCGAA 1 cut(s) 476
Bsp143I GATC 5 cut(s) 51, 78, 285, 379, 574
BspACI CCGC 2 cut(s) 303, 422
BspCNI CTCAG 1 cut(s) 540
BspLI GGNNCC 3 cut(s) 87, 381, 431
BspPI GGATC 5 cut(s) 59, 86, 293, 374, 387
BspT104I TTCGAA 1 cut(s) 476
BspT107I GGYRCC 1 cut(s) 429
BsrBI CCGCTC 1 cut(s) 422
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 5 cut(s) 51, 78, 285, 379, 574
Bst2UI CCWGG 1 cut(s) 434
Bst6I CTCTTC 1 cut(s) 509
BstBI TTCGAA 1 cut(s) 476
BstC8I GCNNGC 1 cut(s) 542
BstDEI CTNAG 1 cut(s) 548
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 5 cut(s) 54, 81, 288, 382, 577
BstMBI GATC 5 cut(s) 51, 78, 285, 379, 574
BstMWI GCNNNNNNNGC 2 cut(s) 178, 229
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 1 cut(s) 445
BstSCI CCNGG 2 cut(s) 223, 432
BstV1I GCAGC 4 cut(s) 90, 173, 184, 288
BstV2I GAAGAC 1 cut(s) 206
BstX2I RGATCY 2 cut(s) 51, 379
BstYI RGATCY 2 cut(s) 51, 379
BtsCI GGATG 1 cut(s) 143
BtsI GCAGTG 1 cut(s) 188
BtsIMutI CAGTG 2 cut(s) 188, 545
Cac8I GCNNGC 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 85
Csp6I GTAC 2 cut(s) 430, 571
CviAII CATG 5 cut(s) 115, 145, 280, 353, 442
CviJI RGCY 5 cut(s) 67, 223, 232, 408, 544
CviKI_1 RGCY 5 cut(s) 67, 223, 232, 408, 544
CviQI GTAC 2 cut(s) 430, 571
DdeI CTNAG 1 cut(s) 548
DpnI GATC 5 cut(s) 53, 80, 287, 381, 576
DpnII GATC 5 cut(s) 51, 78, 285, 379, 574
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
EciI GGCGGA 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 432
FaeI CATG 5 cut(s) 118, 148, 283, 356, 445
FaiI YATR 7 cut(s) 40, 116, 135, 146, 281, 354, 443
FaqI GGGAC 1 cut(s) 71
FatI CATG 5 cut(s) 114, 144, 279, 352, 441
FauI CCCGC 1 cut(s) 415
Fnu4HI GCNGC 4 cut(s) 104, 173, 187, 277
FokI GGATG 1 cut(s) 150
Fsp4HI GCNGC 4 cut(s) 104, 173, 187, 277
FspBI CTAG 1 cut(s) 48
GluI GCNGC 4 cut(s) 104, 173, 187, 277
GsaI CCCAGC 1 cut(s) 67
HapII CCGG 2 cut(s) 225, 363
Hin1II CATG 5 cut(s) 118, 148, 283, 356, 445
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 2 cut(s) 290, 520
HpaII CCGG 2 cut(s) 225, 363
HphI GGTGA 1 cut(s) 285
Hpy188I TCNGA 2 cut(s) 237, 559
Hpy188III TCNNGA 3 cut(s) 417, 566, 578
HpyAV CCTTC 3 cut(s) 420, 444, 488
HpyCH4V TGCA 5 cut(s) 4, 141, 189, 510, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 229
HpyF3I CTNAG 1 cut(s) 548
Hsp92II CATG 5 cut(s) 118, 148, 283, 356, 445
KpnI GGTACC 1 cut(s) 433
Kzo9I GATC 5 cut(s) 51, 78, 285, 379, 574
LpnPI CCDG 7 cut(s) 77, 238, 309, 376, 394, 419, 446
Lsp1109I GCAGC 4 cut(s) 90, 173, 184, 288
LweI GCATC 1 cut(s) 128
MaeI CTAG 1 cut(s) 48
MaeIII GTNAC 3 cut(s) 291, 332, 397
MalI GATC 5 cut(s) 53, 80, 287, 381, 576
MbiI CCGCTC 1 cut(s) 422
MboI GATC 5 cut(s) 51, 78, 285, 379, 574
MboII GAAGA 3 cut(s) 211, 479, 526
MfeI CAATTG 1 cut(s) 586
MflI RGATCY 2 cut(s) 51, 379
MluCI AATT 2 cut(s) 472, 586
MlyI GAGTC 1 cut(s) 299
MnlI CCTC 5 cut(s) 276, 457, 493, 510, 524
MseI TTAA 2 cut(s) 392, 446
MspI CCGG 2 cut(s) 225, 363
MspR9I CCNGG 2 cut(s) 225, 434
MunI CAATTG 1 cut(s) 586
MvaI CCWGG 1 cut(s) 434
MwoI GCNNNNNNNGC 2 cut(s) 178, 229
NciI CCSGG 1 cut(s) 225
NdeII GATC 5 cut(s) 51, 78, 285, 379, 574
NlaIII CATG 5 cut(s) 118, 148, 283, 356, 445
NlaIV GGNNCC 3 cut(s) 87, 381, 431
NmuCI GTSAC 2 cut(s) 291, 332
NspI RCATGY 1 cut(s) 445
NspV TTCGAA 1 cut(s) 476
PaeR7I CTCGAG 1 cut(s) 566
PciI ACATGT 1 cut(s) 441
PfeI GAWTC 1 cut(s) 520
PkrI GCNGC 4 cut(s) 105, 174, 188, 278
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
PpuMI RGGWCCY 1 cut(s) 85
PscI ACATGT 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 432
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 3 cut(s) 87, 381, 431
PspPI GGNCC 1 cut(s) 85
PspPPI RGGWCCY 1 cut(s) 85
PsuI RGATCY 2 cut(s) 51, 379
RsaI GTAC 2 cut(s) 431, 572
RsaNI GTAC 2 cut(s) 430, 571
SaqAI TTAA 2 cut(s) 392, 446
SatI GCNGC 4 cut(s) 104, 173, 187, 277
Sau3AI GATC 5 cut(s) 51, 78, 285, 379, 574
Sau96I GGNCC 1 cut(s) 85
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 2 cut(s) 225, 434
SetI ASST 4 cut(s) 87, 234, 431, 435
SfaNI GCATC 1 cut(s) 128
Sfr274I CTCGAG 1 cut(s) 566
SfuI TTCGAA 1 cut(s) 476
SinI GGWCC 1 cut(s) 85
SlaI CTCGAG 1 cut(s) 566
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 2 cut(s) 472, 586
SsiI CCGC 2 cut(s) 303, 422
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 223, 432
TaqI TCGA 5 cut(s) 288, 386, 416, 476, 567
TasI AATT 2 cut(s) 472, 586
TfiI GAWTC 1 cut(s) 520
Tru1I TTAA 2 cut(s) 392, 446
Tru9I TTAA 2 cut(s) 392, 446
TscAI CASTG 2 cut(s) 188, 552
TseFI GTSAC 2 cut(s) 291, 332
TseI GCWGC 4 cut(s) 103, 172, 186, 276
Tsp45I GTSAC 2 cut(s) 291, 332
TspDTI ATGAA 3 cut(s) 27, 161, 329
TspGWI ACGGA 2 cut(s) 218, 239
TspRI CASTG 2 cut(s) 188, 552
VpaK11BI GGWCC 1 cut(s) 85
XceI RCATGY 1 cut(s) 445
XhoI CTCGAG 1 cut(s) 566
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.