RLG00000025295

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017955
Physical Location & Seq
Forward (+)
43669552 .. 43670181
630 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025295

Sequence Viewer

Length: 630 bp
ATGGAGTCCCGCAGAACTAATCCTCCATTATCAAACTTCATTAGCGTCAGGATGAACAAGCAGTCGAAAATACGAATCAAGCTCCCCAAGTTGCTTGTCAATACCATGCAAAAGAGAGTTGATCACCATCATGAAGATCATAACAACAAGAAAAGTGTAAAGACAGTCAGTTTTGTATTCACCAATCCATTTGCCAAGTACAAGTCACCCGAGAAGTCTTCCTCCAAATCCAATGAGGCTAGTAAACGTGGCAGCAAGCTGAGGCCCCCGAAACTCCGTCTACCGCTGCTGGGTTTCCTCACTAGGAAGAAAAGCTTAAATAAGTTGATGAGGAGCGAAACCGACACTACTGGAGTTCATGGCGTTGGGAAACAAGAAAGTTCGGGCCATTTGGTTCGGGCTCTACCGAAACCTATGTTGACCAATTGCAGAAAGCTGAGAATGGCAATGCCGTACATGAGAGCAAGGAAGATGAAGAGTACGGAGAAGTTGGAGGAAGATTCGAAGCAAGAAGATAGCAGTAGCGAGTATGAACTATGCAAGAAGAGGATTCTAATGGGAGGCAAGTGCAAGTCGGGCAGCTTTCACTACGATCAAAATGGGATTTTGTTAAGTGGTGTCCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

23.87

Weight (kDa)

10.35

Isoelectric Point (pI)

58.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017955)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27020
fragaria_vesca FvH4_6g07020
prunus_persica Prupe.6G317300_v2.0.a1
pyrus_communis pycom12g19710
rosa_laevigata RLG00000025295
rosa_roxburghii Rroxscaffold_6G00422640
rosa_rugosa Rorug03G0013900
rosa_samantha Rh3AG074500 Rh3BG076200 Rh3CG075900 Rh3DG078000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 280
AciI CCGC 2 cut(s) 10, 284
AfaI GTAC 3 cut(s) 200, 455, 481
AfiI CCNNNNNNNGG 1 cut(s) 290
AloI GAACNNNNNNTCC 2 cut(s) 7, 39
AluBI AGCT 5 cut(s) 82, 259, 315, 436, 582
AluI AGCT 5 cut(s) 82, 259, 315, 436, 582
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 2 cut(s) 263, 385
ApeKI GCWGC 3 cut(s) 252, 286, 579
AspS9I GGNCC 2 cut(s) 264, 385
AsuHPI GGTGA 3 cut(s) 116, 172, 198
AsuII TTCGAA 1 cut(s) 503
AvaI CYCGRG 1 cut(s) 209
BanII GRGCYC 1 cut(s) 403
BbsI GAAGAC 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 260
BbvI GCAGC 3 cut(s) 264, 273, 591
BccI CCATC 1 cut(s) 135
BceAI ACGGC 1 cut(s) 436
BclI TGATCA 1 cut(s) 121
BfaI CTAG 2 cut(s) 240, 303
BisI GCNGC 3 cut(s) 253, 287, 580
BlsI GCNGC 3 cut(s) 254, 288, 581
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 2 cut(s) 264, 385
BmiI GGNNCC 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 372
Bpu10I CCTNAGC 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 126
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 453
Bse8I GATNNNNATC 1 cut(s) 126
BseGI GGATG 1 cut(s) 57
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 2 cut(s) 251, 428
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 346
BseXI GCAGC 3 cut(s) 264, 273, 591
BseYI CCCAGC 1 cut(s) 289
BshFI GGCC 2 cut(s) 265, 387
BsiHKCI CYCGRG 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 290
BsnI GGCC 2 cut(s) 265, 387
BsoBI CYCGRG 1 cut(s) 209
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 3 cut(s) 121, 136, 592
BspACI CCGC 2 cut(s) 10, 284
BspANI GGCC 2 cut(s) 265, 387
BspCNI CTCAG 2 cut(s) 252, 429
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 266
BspT104I TTCGAA 1 cut(s) 503
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 1 cut(s) 355
BssMI GATC 3 cut(s) 121, 136, 592
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 2 cut(s) 470, 539
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 2 cut(s) 260, 437
BstF5I GGATG 1 cut(s) 57
BstKTI GATC 3 cut(s) 124, 139, 595
BstMBI GATC 3 cut(s) 121, 136, 592
BstMWI GCNNNNNNNGC 1 cut(s) 576
BstV1I GCAGC 3 cut(s) 264, 273, 591
BstV2I GAAGAC 1 cut(s) 210
BsuRI GGCC 2 cut(s) 265, 387
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 257
CciI TCATGA 1 cut(s) 130
Cfr13I GGNCC 2 cut(s) 264, 385
CseI GACGC 1 cut(s) 34
Csp6I GTAC 3 cut(s) 199, 454, 480
CviAII CATG 5 cut(s) 106, 131, 359, 457, 627
CviJI RGCY 9 cut(s) 82, 239, 259, 265, 315, 387, 401, 436, 582
CviKI_1 RGCY 9 cut(s) 82, 239, 259, 265, 315, 387, 401, 436, 582
CviQI GTAC 3 cut(s) 199, 454, 480
DdeI CTNAG 2 cut(s) 260, 437
DpnI GATC 3 cut(s) 123, 138, 594
DpnII GATC 3 cut(s) 121, 136, 592
Eam1104I CTCTTC 2 cut(s) 470, 539
EarI CTCTTC 2 cut(s) 470, 539
Eco24I GRGCYC 1 cut(s) 403
Eco88I CYCGRG 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 403
FaeI CATG 5 cut(s) 109, 134, 362, 460, 630
FaiI YATR 9 cut(s) 107, 132, 141, 360, 416, 458, 531, 538, 628
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FatI CATG 5 cut(s) 105, 130, 358, 456, 626
FauI CCCGC 1 cut(s) 17
FbaI TGATCA 1 cut(s) 121
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 3 cut(s) 253, 287, 580
FokI GGATG 1 cut(s) 64
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 3 cut(s) 253, 287, 580
FspBI CTAG 2 cut(s) 240, 303
GluI GCNGC 3 cut(s) 253, 287, 580
GsaI CCCAGC 1 cut(s) 293
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 2 cut(s) 265, 387
HgaI GACGC 1 cut(s) 34
Hin1II CATG 5 cut(s) 109, 134, 362, 460, 630
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 4 cut(s) 5, 75, 500, 550
HphI GGTGA 3 cut(s) 116, 172, 198
Hpy166II GTNNAC 3 cut(s) 245, 281, 420
Hpy188III TCNNGA 2 cut(s) 49, 131
Hpy8I GTNNAC 3 cut(s) 245, 281, 420
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 4 cut(s) 109, 429, 540, 570
HpyF10VI GCNNNNNNNGC 1 cut(s) 576
HpyF3I CTNAG 2 cut(s) 260, 437
HpySE526I ACGT 1 cut(s) 247
Hsp92II CATG 5 cut(s) 109, 134, 362, 460, 630
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 3 cut(s) 121, 136, 592
LmnI GCTCC 2 cut(s) 87, 333
LpnPI CCDG 3 cut(s) 34, 275, 336
Lsp1109I GCAGC 3 cut(s) 264, 273, 591
MaeI CTAG 2 cut(s) 240, 303
MaeII ACGT 1 cut(s) 247
MaeIII GTNAC 1 cut(s) 204
MalI GATC 3 cut(s) 123, 138, 594
MboI GATC 3 cut(s) 121, 136, 592
MboII GAAGA 8 cut(s) 146, 210, 319, 481, 487, 509, 524, 556
MfeI CAATTG 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 1 cut(s) 424
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 471
MnlI CCTC 9 cut(s) 33, 229, 232, 255, 308, 324, 487, 540, 554
MseI TTAA 2 cut(s) 317, 611
MslI CAYNNNNRTG 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 286
MunI CAATTG 1 cut(s) 424
MwoI GCNNNNNNNGC 1 cut(s) 576
NdeII GATC 3 cut(s) 121, 136, 592
NlaIII CATG 5 cut(s) 109, 134, 362, 460, 630
NlaIV GGNNCC 1 cut(s) 266
NmuCI GTSAC 1 cut(s) 204
NspV TTCGAA 1 cut(s) 503
PagI TCATGA 1 cut(s) 130
PfeI GAWTC 3 cut(s) 75, 500, 550
PkrI GCNGC 3 cut(s) 254, 288, 581
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 266
PspPI GGNCC 2 cut(s) 264, 385
RsaI GTAC 3 cut(s) 200, 455, 481
RsaNI GTAC 3 cut(s) 199, 454, 480
RseI CAYNNNNRTG 1 cut(s) 129
SaqAI TTAA 2 cut(s) 317, 611
SatI GCNGC 3 cut(s) 253, 287, 580
Sau3AI GATC 3 cut(s) 121, 136, 592
Sau96I GGNCC 2 cut(s) 264, 385
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 403
SetI ASST 7 cut(s) 84, 250, 261, 317, 415, 438, 584
SfuI TTCGAA 1 cut(s) 503
SmiMI CAYNNNNRTG 1 cut(s) 129
Sse9I AATT 1 cut(s) 424
SsiI CCGC 2 cut(s) 10, 284
SspMI CTAG 2 cut(s) 240, 303
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 1 cut(s) 250
TaqI TCGA 2 cut(s) 65, 503
TasI AATT 1 cut(s) 424
TatI WGTACW 1 cut(s) 198
TfiI GAWTC 3 cut(s) 75, 500, 550
Tru1I TTAA 2 cut(s) 317, 611
Tru9I TTAA 2 cut(s) 317, 611
TseFI GTSAC 1 cut(s) 204
TseI GCWGC 3 cut(s) 252, 286, 579
Tsp45I GTSAC 1 cut(s) 204
TspDTI ATGAA 6 cut(s) 28, 68, 147, 347, 488, 546
TspGWI ACGGA 2 cut(s) 266, 497
XmiI GTMKAC 1 cut(s) 280
XspI CTAG 2 cut(s) 240, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.