pycom12g22960

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011068
Physical Location & Seq
Reverse (-)
23689663 .. 23690850
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGCCACGGCCGTTCAACTCCTCGTTCCAGCTGCTGGAGATCAACCTGATCTCGGCCCAGGAACTGACGCCGGCATCGAAGGCAATGCGGACGTTCGCCGTCGCGTGGGTCAATCCGCAGAGGAAGCTGACGACACGTGTCGACCAGAACGGCCACACCAATCCGACGTGGAACGAGAAGTTCGTCTTCCGAGTCGACGACGACTTCCTCCGTGACGACACCTCGAAGATCATGATCGAGATCTACGCCTCCGCGTGGCTCCGCGACGTCCTGATCGGGACCGCAGCGGTGGTGGTGAACAATTTGCAGAACAAGTCGAAAATGCGGTTCATGGCGATCCAGCTCCGCCGCCCCTCCGGCCGCCCGCAGGGGATTCTGAACATCGGGCTCGGCCTCCTCGATAGCACAATGCGGAGCATGCCGCTCTACAGCGAGCTCAGCTCCTCCGCTGTCGGGTACTGGGATCTGATGGAGGGGAAGACCGGCAACCACCAGAAGAGCAACGGCCCCAATTCGAAGGACCAAGACAAATTCATCATCTTCCAACGATCTCAGAGCGATCGGACGGGTAGCGATTACGGATTCTCGAAATCCAGGCCTGCATCCTCCATCTGCGACGGCGTCACAAAAAGCAGGCCCCCGAGCTCTATCTGCAACGCCGCCGCATACAAGCCCAAACATCAAGCGTCCTTCTGCAATAGCTCGATTGTGAACGGATCTGAGGTCAGCACCGTGCAAAAGAAGGGGAATGTGAACGGGTCCCTCTGCTCCGACGTCGGGCCCTCGCCGTCTGTGGTTGCGGCTGCGATCGCCAAGGGAATCTACCCGCTGCCGTACACCGGGGCCCGCGCACCGCCGGCCAATGATGCCGGGAGCTCGTTGCTTGACGAATGGACGGACCAAGACAGCGTGGAAGGGTTAAAGACCAAGATCGAACGGTGGCGGACGGAGCTGCCGCCGGTGTACGACTGCGCCAAGAACGCTGCCAAGAGCTCCCAAAATCAGAAGCTACTCCAATCATCGCACCGCCGCCCCAGCAGTCAGAAGACACGGCGGAGACGCGGCAGTGGCCGGGGGTTATTCTCCTGCTTCGCATTGGGCTGCGAGATCTCGATCTCTTGCGGCGGCAAGCCGAAGAAGAAGAAGAGCGGGAAGGTCCACCTCGACGACGACTCGTACTTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

396

Amino Acids

43.39

Weight (kDa)

9.68

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 11 - 107 3.7e-14 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G13350 AT3G04360
fragaria_vesca FvH4_6g01610
malus_domestica MD00G1149000.v1.1 MD04G1232000.v1.1
prunus_persica Prupe.6G351400_v2.0.a1
pyrus_communis pycom04g20540 pycom12g22960
rosa_chinensis RchiOBHm_Chr3g0449421
rosa_laevigata RLG00000025832
rosa_multiflora Rmu_sc0019012.1_g000008 Rmu_sc0019478.1_g000001 Rmu_sc0020017.1_g000003
rosa_roxburghii Rroxscaffold_6G00427380
rosa_rugosa Rorug02G0620500
rosa_samantha Rh3AG018300 Rh3BG018600 Rh3CG017500 Rh3DG019000
rosa_wichuraiana Rw3G001470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 270, 777
AccB7I CCANNNNNTGG 1 cut(s) 34
AccBSI CCGCTC 2 cut(s) 424, 1149
AccI GTMKAC 2 cut(s) 141, 195
AccII CGCG 5 cut(s) 104, 254, 264, 849, 1062
AclWI GGATC 3 cut(s) 331, 471, 724
AcoI YGGCCR 5 cut(s) 8, 151, 358, 858, 1069
AcsI RAATTY 1 cut(s) 530
AcvI CACGTG 1 cut(s) 137
AcyI GRCGYC 4 cut(s) 68, 267, 621, 774
AfaI GTAC 4 cut(s) 458, 836, 965, 1178
AfiI CCNNNNNNNGG 8 cut(s) 34, 52, 105, 255, 367, 453, 777, 839
AflIII ACRYGT 2 cut(s) 134, 136
AgsI TTSAA 1 cut(s) 16
AjiI CACGTC 1 cut(s) 168
AjnI CCWGG 2 cut(s) 57, 593
Alw21I GWGCWC 4 cut(s) 438, 647, 878, 995
Alw26I GTCTC 1 cut(s) 1051
AlwI GGATC 3 cut(s) 331, 471, 724
AlwNI CAGNNNCTG 2 cut(s) 34, 64
Ama87I CYCGRG 1 cut(s) 640
ApaI GGGCCC 2 cut(s) 783, 847
ApeKI GCWGC 7 cut(s) 31, 284, 803, 829, 952, 983, 1101
ApoI RAATTY 1 cut(s) 530
AsiSI GCGATCGC 1 cut(s) 810
AspLEI GCGC 2 cut(s) 851, 974
AsuC2I CCSGG 3 cut(s) 841, 871, 1073
AsuHPI GGTGA 1 cut(s) 307
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 640
AvaII GGWCC 5 cut(s) 279, 520, 759, 898, 1156
BaeGI GKGCMC 2 cut(s) 783, 847
BanII GRGCYC 7 cut(s) 390, 438, 647, 783, 847, 878, 995
BbrPI CACGTG 1 cut(s) 137
BbsI GAAGAC 3 cut(s) 178, 485, 1052
Bbv12I GWGCWC 4 cut(s) 438, 647, 878, 995
BbvI GCAGC 7 cut(s) 18, 296, 790, 816, 939, 970, 1088
BccI CCATC 2 cut(s) 463, 617
BceAI ACGGC 8 cut(s) 23, 83, 166, 520, 634, 772, 817, 1067
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 2 cut(s) 59, 595
BcnI CCSGG 3 cut(s) 841, 871, 1073
BcoDI GTCTC 1 cut(s) 1051
BfmI CTRYAG 1 cut(s) 427
BglI GCCNNNNNGGC 1 cut(s) 357
BglII AGATCT 2 cut(s) 240, 1107
BlpI GCTNAGC 1 cut(s) 437
Bme1390I CCNGG 5 cut(s) 59, 595, 841, 871, 1073
Bme18I GGWCC 5 cut(s) 279, 520, 759, 898, 1156
BmeT110I CYCGRG 1 cut(s) 640
BmgBI CACGTC 1 cut(s) 168
BmiI GGNNCC 9 cut(s) 260, 280, 508, 638, 760, 761, 781, 844, 845
BmrFI CCNGG 5 cut(s) 59, 595, 841, 871, 1073
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 3 cut(s) 83, 611, 856
BmuI ACTGGG 1 cut(s) 469
BoxI GACNNNNGTC 1 cut(s) 137
BpiI GAAGAC 3 cut(s) 178, 485, 1052
BplI GAGNNNNNCTC 2 cut(s) 425, 457
BpmI CTGGAG 1 cut(s) 56
Bpu1102I GCTNAGC 1 cut(s) 437
Bpu14I TTCGAA 1 cut(s) 515
BpuMI CCSGG 3 cut(s) 841, 871, 1073
BsaAI YACGTR 1 cut(s) 137
BsaBI GATNNNNATC 3 cut(s) 233, 239, 1112
BsaHI GRCGYC 4 cut(s) 68, 267, 621, 774
BsaJI CCNNGG 5 cut(s) 5, 57, 813, 840, 1072
Bsc4I CCNNNNNNNGG 8 cut(s) 34, 52, 105, 255, 367, 453, 777, 839
Bse118I RCCGGY 4 cut(s) 70, 482, 856, 958
Bse1I ACTGG 1 cut(s) 464
Bse3DI GCAATG 1 cut(s) 90
Bse8I GATNNNNATC 3 cut(s) 233, 239, 1112
BseBI CCWGG 2 cut(s) 59, 595
BseDI CCNNGG 5 cut(s) 5, 57, 813, 840, 1072
BseGI GGATG 1 cut(s) 602
BseJI GATNNNNATC 3 cut(s) 233, 239, 1112
BseLI CCNNNNNNNGG 8 cut(s) 34, 52, 105, 255, 367, 453, 777, 839
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 3 cut(s) 451, 566, 711
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 3 cut(s) 10, 386, 433
BseSI GKGCMC 2 cut(s) 783, 847
BseX3I CGGCCG 2 cut(s) 8, 358
BseXI GCAGC 7 cut(s) 18, 296, 790, 816, 939, 970, 1088
BseYI CCCAGC 1 cut(s) 1034
Bsh1236I CGCG 5 cut(s) 104, 254, 264, 849, 1062
Bsh1285I CGRYCG 4 cut(s) 11, 361, 562, 810
BsiEI CGRYCG 4 cut(s) 11, 361, 562, 810
BsiHKAI GWGCWC 4 cut(s) 438, 647, 878, 995
BsiHKCI CYCGRG 1 cut(s) 640
BsiSI CCGG 8 cut(s) 71, 357, 483, 840, 857, 870, 959, 1072
BslFI GGGAC 2 cut(s) 292, 745
BslI CCNNNNNNNGG 8 cut(s) 34, 52, 105, 255, 367, 453, 777, 839
BsmAI GTCTC 1 cut(s) 1051
BsmBI CGTCTC 1 cut(s) 1051
BsmFI GGGAC 2 cut(s) 292, 745
BsoBI CYCGRG 1 cut(s) 640
Bsp119I TTCGAA 1 cut(s) 515
Bsp120I GGGCCC 2 cut(s) 779, 843
Bsp1286I GDGCHC 7 cut(s) 390, 438, 647, 783, 847, 878, 995
Bsp1720I GCTNAGC 1 cut(s) 437
BspCNI CTCAG 3 cut(s) 450, 565, 712
BspFNI CGCG 5 cut(s) 104, 254, 264, 849, 1062
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 9 cut(s) 260, 280, 508, 638, 760, 761, 781, 844, 845
BspPI GGATC 3 cut(s) 331, 471, 724
BspQI GCTCTTC 2 cut(s) 491, 1139
BspT104I TTCGAA 1 cut(s) 515
BsrBI CCGCTC 2 cut(s) 424, 1149
BsrDI GCAATG 1 cut(s) 90
BsrFI RCCGGY 4 cut(s) 70, 482, 856, 958
BsrI ACTGG 1 cut(s) 464
BssAI RCCGGY 4 cut(s) 70, 482, 856, 958
BssECI CCNNGG 5 cut(s) 5, 57, 813, 840, 1072
BssNI GRCGYC 4 cut(s) 68, 267, 621, 774
BssT1I CCWWGG 1 cut(s) 813
Bst2UI CCWGG 2 cut(s) 59, 595
Bst4CI ACNGT 3 cut(s) 733, 939, 1185
Bst6I CTCTTC 2 cut(s) 491, 1139
BstACI GRCGYC 4 cut(s) 68, 267, 621, 774
BstBAI YACGTR 1 cut(s) 137
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 9 cut(s) 72, 365, 419, 434, 600, 635, 847, 858, 1130
BstDEI CTNAG 3 cut(s) 437, 552, 720
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 1 cut(s) 602
BstFNI CGCG 5 cut(s) 104, 254, 264, 849, 1062
BstHHI GCGC 2 cut(s) 851, 974
BstMAI GTCTC 1 cut(s) 1051
BstMCI CGRYCG 4 cut(s) 11, 361, 562, 810
BstNI CCWGG 2 cut(s) 59, 595
BstNSI RCATGY 1 cut(s) 421
BstPAI GACNNNNGTC 1 cut(s) 137
BstSCI CCNGG 5 cut(s) 57, 593, 839, 869, 1071
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 2 cut(s) 783, 847
BstUI CGCG 5 cut(s) 104, 254, 264, 849, 1062
BstV1I GCAGC 7 cut(s) 18, 296, 790, 816, 939, 970, 1088
BstV2I GAAGAC 3 cut(s) 178, 485, 1052
BstX2I RGATCY 4 cut(s) 240, 463, 716, 1107
BstYI RGATCY 4 cut(s) 240, 463, 716, 1107
BstZI CGGCCG 2 cut(s) 8, 358
BtgI CCRYGG 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 1005
BtrI CACGTC 1 cut(s) 168
BtsCI GGATG 1 cut(s) 602
BtsI GCAGTG 1 cut(s) 1072
BtsIMutI CAGTG 1 cut(s) 1072
Cac8I GCNNGC 9 cut(s) 72, 365, 419, 434, 600, 635, 847, 858, 1130
CaiI CAGNNNCTG 2 cut(s) 34, 64
CciI TCATGA 1 cut(s) 231
CfoI GCGC 2 cut(s) 851, 974
Cfr10I RCCGGY 4 cut(s) 70, 482, 856, 958
CseI GACGC 4 cut(s) 76, 610, 675, 1068
Csp6I GTAC 4 cut(s) 457, 835, 964, 1177
CviAII CATG 3 cut(s) 232, 331, 418
CviQI GTAC 4 cut(s) 457, 835, 964, 1177
DdeI CTNAG 3 cut(s) 437, 552, 720
EaeI YGGCCR 5 cut(s) 8, 151, 358, 858, 1069
EagI CGGCCG 2 cut(s) 8, 358
Eam1104I CTCTTC 2 cut(s) 491, 1139
EarI CTCTTC 2 cut(s) 491, 1139
EciI GGCGGA 3 cut(s) 335, 958, 1069
Ecl136II GAGCTC 4 cut(s) 436, 645, 876, 993
EclXI CGGCCG 2 cut(s) 8, 358
Eco130I CCWWGG 1 cut(s) 813
Eco147I AGGCCT 1 cut(s) 598
Eco24I GRGCYC 7 cut(s) 390, 438, 647, 783, 847, 878, 995
Eco47I GGWCC 5 cut(s) 279, 520, 759, 898, 1156
Eco52I CGGCCG 2 cut(s) 8, 358
Eco53kI GAGCTC 4 cut(s) 436, 645, 876, 993
Eco72I CACGTG 1 cut(s) 137
Eco88I CYCGRG 1 cut(s) 640
EcoICRI GAGCTC 4 cut(s) 436, 645, 876, 993
EcoO109I RGGNCCY 4 cut(s) 636, 759, 780, 843
EcoRII CCWGG 2 cut(s) 57, 593
EcoT14I CCWWGG 1 cut(s) 813
EcoT38I GRGCYC 7 cut(s) 390, 438, 647, 783, 847, 878, 995
ErhI CCWWGG 1 cut(s) 813
Esp3I CGTCTC 1 cut(s) 1051
FaeI CATG 3 cut(s) 235, 334, 421
FaiI YATR 4 cut(s) 233, 332, 419, 667
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 2 cut(s) 292, 745
FatI CATG 3 cut(s) 231, 330, 417
FauI CCCGC 4 cut(s) 372, 834, 854, 1142
FblI GTMKAC 2 cut(s) 141, 195
FokI GGATG 1 cut(s) 589
FriOI GRGCYC 7 cut(s) 390, 438, 647, 783, 847, 878, 995
GlaI GCGC 2 cut(s) 850, 973
GsaI CCCAGC 1 cut(s) 1038
GsuI CTGGAG 1 cut(s) 56
HapII CCGG 8 cut(s) 71, 357, 483, 840, 857, 870, 959, 1072
HgaI GACGC 4 cut(s) 76, 610, 675, 1068
HhaI GCGC 2 cut(s) 851, 974
Hin1I GRCGYC 4 cut(s) 68, 267, 621, 774
Hin1II CATG 3 cut(s) 235, 334, 421
Hin6I GCGC 2 cut(s) 849, 972
HinP1I GCGC 2 cut(s) 849, 972
HincII GTYRAC 2 cut(s) 142, 196
HindII GTYRAC 2 cut(s) 142, 196
HinfI GANTC 5 cut(s) 192, 373, 582, 819, 1172
HpaII CCGG 8 cut(s) 71, 357, 483, 840, 857, 870, 959, 1072
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 8 cut(s) 142, 196, 298, 712, 754, 837, 964, 1159
Hpy188III TCNNGA 6 cut(s) 232, 238, 271, 277, 586, 1111
Hpy8I GTNNAC 8 cut(s) 142, 196, 298, 712, 754, 837, 964, 1159
HpyAV CCTTC 6 cut(s) 73, 511, 700, 736, 908, 1147
HpyCH4III ACNGT 3 cut(s) 733, 939, 1185
HpyCH4IV ACGT 5 cut(s) 92, 136, 167, 267, 774
HpyCH4V TGCA 5 cut(s) 307, 602, 654, 696, 736
HpyF3I CTNAG 3 cut(s) 437, 552, 720
HpySE526I ACGT 5 cut(s) 92, 136, 167, 267, 774
Hsp92I GRCGYC 4 cut(s) 68, 267, 621, 774
Hsp92II CATG 3 cut(s) 235, 334, 421
HspAI GCGC 2 cut(s) 849, 972
KflI GGGWCCC 1 cut(s) 759
KroI GCCGGC 2 cut(s) 70, 856
KroNI GCCGGC 2 cut(s) 72, 858
LguI GCTCTTC 2 cut(s) 491, 1139
LmnI GCTCC 8 cut(s) 264, 348, 414, 446, 773, 873, 949, 998
Lsp1109I GCAGC 7 cut(s) 18, 296, 790, 816, 939, 970, 1088
LweI GCATC 3 cut(s) 83, 611, 856
MaeII ACGT 5 cut(s) 92, 136, 167, 267, 774
MaeIII GTNAC 2 cut(s) 212, 622
MbiI CCGCTC 2 cut(s) 424, 1149
MflI RGATCY 4 cut(s) 240, 463, 716, 1107
MhlI GDGCHC 7 cut(s) 390, 438, 647, 783, 847, 878, 995
MluCI AATT 3 cut(s) 301, 511, 530
MlyI GAGTC 2 cut(s) 201, 1166
MmeI TCCRAC 3 cut(s) 188, 568, 795
MroNI GCCGGC 2 cut(s) 70, 856
MseI TTAA 1 cut(s) 920
MspA1I CMGCKG 4 cut(s) 31, 287, 449, 829
MspI CCGG 8 cut(s) 71, 357, 483, 840, 857, 870, 959, 1072
MspR9I CCNGG 5 cut(s) 59, 595, 841, 871, 1073
MvaI CCWGG 2 cut(s) 59, 595
MvnI CGCG 5 cut(s) 104, 254, 264, 849, 1062
NaeI GCCGGC 2 cut(s) 72, 858
NciI CCSGG 3 cut(s) 841, 871, 1073
NgoMIV GCCGGC 2 cut(s) 70, 856
NlaIII CATG 3 cut(s) 235, 334, 421
NlaIV GGNNCC 9 cut(s) 260, 280, 508, 638, 760, 761, 781, 844, 845
NmeAIII GCCGAG 2 cut(s) 32, 369
NmuCI GTSAC 2 cut(s) 212, 622
NspI RCATGY 1 cut(s) 421
NspV TTCGAA 1 cut(s) 515
PaeI GCATGC 1 cut(s) 421
PagI TCATGA 1 cut(s) 231
PceI AGGCCT 1 cut(s) 598
PciSI GCTCTTC 2 cut(s) 491, 1139
PcsI WCGNNNNNNNCGW 3 cut(s) 74, 180, 396
PdiI GCCGGC 2 cut(s) 72, 858
PfeI GAWTC 3 cut(s) 373, 582, 819
PflFI GACNNNGTC 1 cut(s) 620
PflMI CCANNNNNTGG 1 cut(s) 34
Ple19I CGATCG 2 cut(s) 562, 810
PleI GAGTC 2 cut(s) 200, 1166
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
PpsI GAGTC 2 cut(s) 200, 1166
Ppu21I YACGTR 1 cut(s) 137
PpuMI RGGWCCY 1 cut(s) 759
PshAI GACNNNNGTC 1 cut(s) 137
Psp124BI GAGCTC 4 cut(s) 438, 647, 878, 995
Psp5II RGGWCCY 1 cut(s) 759
Psp6I CCWGG 2 cut(s) 57, 593
PspCI CACGTG 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 1034
PspGI CCWGG 2 cut(s) 57, 593
PspN4I GGNNCC 9 cut(s) 260, 280, 508, 638, 760, 761, 781, 844, 845
PspOMI GGGCCC 2 cut(s) 779, 843
PspPPI RGGWCCY 1 cut(s) 759
PstNI CAGNNNCTG 2 cut(s) 34, 64
PsuI RGATCY 4 cut(s) 240, 463, 716, 1107
PsyI GACNNNGTC 1 cut(s) 620
PvuI CGATCG 2 cut(s) 562, 810
PvuII CAGCTG 1 cut(s) 31
RgaI GCGATCGC 1 cut(s) 810
RsaI GTAC 4 cut(s) 458, 836, 965, 1178
RsaNI GTAC 4 cut(s) 457, 835, 964, 1177
SacI GAGCTC 4 cut(s) 438, 647, 878, 995
SalI GTCGAC 2 cut(s) 140, 194
SapI GCTCTTC 2 cut(s) 491, 1139
SaqAI TTAA 1 cut(s) 920
SchI GAGTC 2 cut(s) 201, 1166
ScrFI CCNGG 5 cut(s) 59, 595, 841, 871, 1073
SduI GDGCHC 7 cut(s) 390, 438, 647, 783, 847, 878, 995
SfaAI GCGATCGC 1 cut(s) 810
SfaNI GCATC 3 cut(s) 83, 611, 856
SfcI CTRYAG 1 cut(s) 427
SfuI TTCGAA 1 cut(s) 515
SgfI GCGATCGC 1 cut(s) 810
SgrAI CRCCGGYG 1 cut(s) 958
SinI GGWCC 5 cut(s) 279, 520, 759, 898, 1156
SphI GCATGC 1 cut(s) 421
Sse9I AATT 3 cut(s) 301, 511, 530
SseBI AGGCCT 1 cut(s) 598
SstI GAGCTC 4 cut(s) 438, 647, 878, 995
StuI AGGCCT 1 cut(s) 598
StyD4I CCNGG 5 cut(s) 57, 593, 839, 869, 1071
StyI CCWWGG 1 cut(s) 813
TaaI ACNGT 3 cut(s) 733, 939, 1185
TaiI ACGT 5 cut(s) 95, 139, 170, 270, 777
TasI AATT 3 cut(s) 301, 511, 530
TfiI GAWTC 3 cut(s) 373, 582, 819
Tru1I TTAA 1 cut(s) 920
Tru9I TTAA 1 cut(s) 920
TscAI CASTG 1 cut(s) 1072
TseFI GTSAC 2 cut(s) 212, 622
TseI GCWGC 7 cut(s) 31, 284, 803, 829, 952, 983, 1101
Tsp45I GTSAC 2 cut(s) 212, 622
TspDTI ATGAA 2 cut(s) 319, 523
TspGWI ACGGA 5 cut(s) 200, 594, 729, 911, 962
TspRI CASTG 1 cut(s) 1072
Tth111I GACNNNGTC 1 cut(s) 620
Van91I CCANNNNNTGG 1 cut(s) 34
VpaK11BI GGWCC 5 cut(s) 279, 520, 759, 898, 1156
XapI RAATTY 1 cut(s) 530
XceI RCATGY 1 cut(s) 421
XmiI GTMKAC 2 cut(s) 141, 195
ZraI GACGTC 2 cut(s) 268, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.