pycom13g05240

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0018482
Physical Location & Seq
Reverse (-)
3485666 .. 3486785
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGTTTCTCACAACATTGTTGATGGCAGCTAAAGACAGAAAGCCAATGCCAAGTCAAAATTCTCATTTTTGTCATCTTATGGAATTAAAAGCAATCAGGCCTCTTTTATGTATACATGAATCTGTAGCTCAGGTTGATTCTCTCAATTTTCTCACAATAATGGACCTGCCAGAGGACTCCTCTTTTTTCAGCTCTGACGATTTCAGGGCTAGTGTGCTGGTGACGGTGATTGAAACTCTACGTGAGTTTGTCATCATTTATGAAGGATTCAGTTCTTTTCCTGAAATCTTTTTGCAAATTTCAGTATTGTTGCTTGAAGTGGCGGAACAGGATAATATGCCACAAGTATTAACAGACAAATTGAAGGATGTTGCTGAACTGATTAAGACAGAAGCAGATAAACATCACATTCTGCGGCAACCACTTCAGATGCGGAAGCAAAAGCCGGTGGCTATCAAAATGCTCAATCCGAAATTTGAGGAGAACTTTGTTAAGGGCAGAGACTATGATCCAGATCGGGAACGAGCCGAGCGAAGAAAGATGAAGAAACTTTTGAAGCAAGAAGCTAAAGGGGCTATTCGTGAACTGCGTAAGGATAATTCTTTCCTTTACGAGGTGAAGGCAAGGGATAAGGCGCTGATGGAAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop14

Protein Analysis

218

Amino Acids

25.33

Weight (kDa)

6.26

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop14 PF04147 2 - 215 1.6e-51 Nop14-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018482)

Species Orthologous Gene IDs
malus_domestica MD13G1058800.v1.1
pyrus_communis pycom13g05240
rosa_chinensis RchiOBHm_Chr1g0340811
rosa_laevigata RLG00000029046
rosa_multiflora Rmu_co8097760.1_g000001 Rmu_sc0002200.1_g000056
rosa_roxburghii Rroxscaffold_4G00311500
rosa_samantha Rh1BG144200 Rh1CG164000 Rh1DG175900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 174
AccI GTMKAC 1 cut(s) 112
AciI CCGC 3 cut(s) 323, 415, 433
AclWI GGATC 1 cut(s) 503
AcsI RAATTY 3 cut(s) 58, 297, 473
AcuI CTGAAG 1 cut(s) 410
AfiI CCNNNNNNNGG 2 cut(s) 172, 613
AgsI TTSAA 4 cut(s) 233, 317, 364, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AluBI AGCT 4 cut(s) 29, 128, 192, 566
AluI AGCT 4 cut(s) 29, 128, 192, 566
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 1 cut(s) 503
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 1 cut(s) 26
ApoI RAATTY 3 cut(s) 58, 297, 473
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 1 cut(s) 163
AsuHPI GGTGA 3 cut(s) 232, 238, 628
AvaII GGWCC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 38
BccI CCATC 2 cut(s) 16, 634
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 1 cut(s) 210
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 638
BfuAI ACCTGC 1 cut(s) 174
BisI GCNGC 2 cut(s) 27, 416
BlsI GCNGC 2 cut(s) 28, 417
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 163
BmsI GCATC 1 cut(s) 420
BplI GAGNNNNNCTC 2 cut(s) 164, 196
Bpu10I CCTNAGC 1 cut(s) 129
BsaAI YACGTR 1 cut(s) 242
BsaBI GATNNNNATC 2 cut(s) 402, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 613
Bse118I RCCGGY 1 cut(s) 445
Bse8I GATNNNNATC 2 cut(s) 402, 513
BseGI GGATG 1 cut(s) 373
BseJI GATNNNNATC 2 cut(s) 402, 513
BseLI CCNNNNNNNGG 2 cut(s) 172, 613
BseMII CTCAG 1 cut(s) 143
BseRI GAGGAG 2 cut(s) 169, 494
BseXI GCAGC 1 cut(s) 38
BshFI GGCC 1 cut(s) 100
BsiSI CCGG 1 cut(s) 446
BslI CCNNNNNNNGG 2 cut(s) 172, 613
BsmAI GTCTC 1 cut(s) 495
BsnI GGCC 1 cut(s) 100
Bsp143I GATC 2 cut(s) 508, 514
BspACI CCGC 3 cut(s) 323, 415, 433
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 1 cut(s) 142
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 445
BssAI RCCGGY 1 cut(s) 445
BssMI GATC 2 cut(s) 508, 514
BssNAI GTATAC 1 cut(s) 113
Bst1107I GTATAC 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 226
Bst6I CTCTTC 1 cut(s) 642
BstBAI YACGTR 1 cut(s) 242
BstDEI CTNAG 1 cut(s) 129
BstENI CCTNNNNNAGG 2 cut(s) 170, 611
BstF5I GGATG 1 cut(s) 373
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 2 cut(s) 511, 517
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 2 cut(s) 508, 514
BstMWI GCNNNNNNNGC 1 cut(s) 572
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 38
BstZ17I GTATAC 1 cut(s) 113
BsuRI GGCC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 373
BveI ACCTGC 1 cut(s) 174
CfoI GCGC 1 cut(s) 637
Cfr10I RCCGGY 1 cut(s) 445
Cfr13I GGNCC 1 cut(s) 163
CviAII CATG 1 cut(s) 116
DdeI CTNAG 1 cut(s) 129
DpnI GATC 2 cut(s) 510, 516
DpnII GATC 2 cut(s) 508, 514
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
EciI GGCGGA 1 cut(s) 338
Eco147I AGGCCT 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 410
EcoNI CCTNNNNNAGG 2 cut(s) 170, 611
FaeI CATG 1 cut(s) 119
FaiI YATR 7 cut(s) 80, 109, 113, 117, 261, 338, 507
FatI CATG 1 cut(s) 115
FblI GTMKAC 1 cut(s) 112
Fnu4HI GCNGC 2 cut(s) 27, 416
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 2 cut(s) 27, 416
FspBI CTAG 1 cut(s) 210
GlaI GCGC 1 cut(s) 636
GluI GCNGC 2 cut(s) 27, 416
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 1 cut(s) 100
HapII CCGG 1 cut(s) 446
HhaI GCGC 1 cut(s) 637
Hin1II CATG 1 cut(s) 119
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HinfI GANTC 4 cut(s) 119, 137, 176, 267
HpaII CCGG 1 cut(s) 446
HphI GGTGA 3 cut(s) 232, 238, 628
Hpy166II GTNNAC 2 cut(s) 113, 584
Hpy188I TCNGA 3 cut(s) 196, 429, 471
Hpy188III TCNNGA 4 cut(s) 281, 512, 518, 581
Hpy8I GTNNAC 2 cut(s) 113, 584
HpyAV CCTTC 3 cut(s) 257, 358, 613
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4IV ACGT 1 cut(s) 241
HpyCH4V TGCA 1 cut(s) 295
HpyF10VI GCNNNNNNNGC 1 cut(s) 572
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 1 cut(s) 241
Hsp92II CATG 1 cut(s) 119
HspAI GCGC 1 cut(s) 635
Kzo9I GATC 2 cut(s) 508, 514
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 1 cut(s) 420
MaeI CTAG 1 cut(s) 210
MaeII ACGT 1 cut(s) 241
MaeIII GTNAC 1 cut(s) 220
MalI GATC 2 cut(s) 510, 516
MboI GATC 2 cut(s) 508, 514
MboII GAAGA 2 cut(s) 546, 556
MluCI AATT 7 cut(s) 58, 83, 145, 297, 359, 473, 598
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 5 cut(s) 111, 166, 190, 472, 607
MseI TTAA 4 cut(s) 86, 350, 384, 492
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 572
NdeII GATC 2 cut(s) 508, 514
NlaIII CATG 1 cut(s) 119
NmeAIII GCCGAG 1 cut(s) 553
NmuCI GTSAC 1 cut(s) 220
PceI AGGCCT 1 cut(s) 100
PcsI WCGNNNNNNNCGW 2 cut(s) 529, 586
PfeI GAWTC 3 cut(s) 119, 137, 267
PkrI GCNGC 2 cut(s) 28, 417
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
Ppu21I YACGTR 1 cut(s) 242
PspPI GGNCC 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 4 cut(s) 86, 350, 384, 492
SatI GCNGC 2 cut(s) 27, 416
Sau3AI GATC 2 cut(s) 508, 514
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 170
SetI ASST 8 cut(s) 31, 130, 135, 168, 194, 244, 568, 618
SfaNI GCATC 1 cut(s) 420
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 7 cut(s) 58, 83, 145, 297, 359, 473, 598
SseBI AGGCCT 1 cut(s) 100
SsiI CCGC 3 cut(s) 323, 415, 433
SspMI CTAG 1 cut(s) 210
StuI AGGCCT 1 cut(s) 100
TaaI ACNGT 1 cut(s) 226
TaiI ACGT 1 cut(s) 244
TasI AATT 7 cut(s) 58, 83, 145, 297, 359, 473, 598
TauI GCSGC 1 cut(s) 418
TfiI GAWTC 3 cut(s) 119, 137, 267
Tru1I TTAA 4 cut(s) 86, 350, 384, 492
Tru9I TTAA 4 cut(s) 86, 350, 384, 492
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 26
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 3 cut(s) 132, 276, 557
VpaK11BI GGWCC 1 cut(s) 163
XagI CCTNNNNNAGG 2 cut(s) 170, 611
XapI RAATTY 3 cut(s) 58, 297, 473
XmiI GTMKAC 1 cut(s) 112
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.