pycom13g12630

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008992
Physical Location & Seq
Reverse (-)
8806183 .. 8809981
3799 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1386 bp
ATGCAAGCAATGTGGAAGGAAGATATCATTACCACAAGAGAAACAAAAGCGCATCCCCTCCTCCTACCCCTGCAGAAGATCGGGCCAGTTGCTCCTTCAACCCCGAGCATTACTCCTCCTACCCCTTCTCCACATGTTCCCTCAGACCCTTACCCAAATGACCCTGGAAACAACTCTAGTAATTCTTCAGATTGTGTGTTCAATGTCATGGATTATGGGGCAGTTGGAGATGGCTCTGCTGATGACACTGCTGCATTTAGACAGGCTTGGAAAGAAGCCTGTGCTGTTGAATCAGGTGTTGTTTTGGCTCCTTCGGACTACTGCTTCAAAATCACCTCAACAATTTTTTCAGGTCCATGCCAGCCAGGACTAGTATTCCAAGTAGACGGGGTTTTGATGCCGCCGGACGGGCCAGAGACGTGGCCTACAAAAGACAGCCTAAACCAGTGGCTTGTGTTTTACAGACTCAACCAAATGACTTTCACTGGTACTGGAACCATCGAAGGCAATGGCCAAAAGTGGTGGGACCTCCCTTGCAAACCTCACAGGGGTCCCAAAGGATCAACACTGCCAGGACCATGTGACAGCCCTGCGATGATCAGATTTTTCATGAGCTCTAATTTGGTGGTGAGAGGCTTAAGAATCCAAAACAGTCCAAAGTTCCACATGAAATTCGATGGCTGTGAAGGAGTACTGATCGAGAAAATATCCATTTCTTCACCAAAGCTTAGCCCCAACACAGATGGGATCCACATAGAGAACACAAAATCTGTTGCAATATACGACTCAATGATTAGCAACGGTGATGACTGCATTTCAATAGGAACTGGGTGTGCAAATGTTGATATAATGGGTGTCACTTGCGGGCCAAGTCACGGGATTAGCATTGGGAGTCTTGGCGTGCACAACTCTCAGGCATGCGTCTCCAACATCACAGTCCGCAACGCAGTCATACGTGAATCAGACAACGGCGTCCGAATCAAGACATGGCAAGGCGGGACTGGCTGCGTATCCGGCATCCTCTTCGAGAACATCCAGATGGAGAATGTGCTCAACTGCCTGCTAGTAGACCAGTACTATTGCCTATCAAAGGGATGCCTAAACCAAACCTCAGCAGTACTAGTCACAGATTTAACATACAGAAACATAAAGGGGACGTACGATGTGAGGACGCCTCCCATTCACTTTGCATGCAGTGACACCGTGGCATGCAGAAATATTACCCTCTCAGAAGTTGAGCTTTACCCACACGAAGGAGAGCTCATGGATGATCCCTTTTGTTGGAATGCTTATGGAATCCAGGAGACTGCTATTATTCCCCCTATTGATTGCTTGCAAGAGGGGGAACCTCAGGCTGCGGCTGAGGTGTCTGAGTATACTTGCTAG

Protein Analysis

462

Amino Acids

49.92

Weight (kDa)

4.81

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 103 - 414 2e-82 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 971
AccI GTMKAC 3 cut(s) 384, 1068, 1376
AciI CCGC 5 cut(s) 401, 864, 940, 996, 1358
AclWI GGATC 4 cut(s) 568, 742, 755, 1265
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 1 cut(s) 671
AcuI CTGAAG 1 cut(s) 171
AcyI GRCGYC 2 cut(s) 972, 1172
AfaI GTAC 5 cut(s) 490, 693, 1076, 1119, 1160
AfiI CCNNNNNNNGG 6 cut(s) 407, 548, 875, 1090, 1253, 1281
AflII CTTAAG 1 cut(s) 637
AflIII ACRYGT 1 cut(s) 133
AgsI TTSAA 5 cut(s) 99, 202, 290, 328, 819
AhlI ACTAGT 2 cut(s) 370, 1120
AjiI CACGTC 1 cut(s) 420
AjnI CCWGG 4 cut(s) 163, 364, 571, 1299
AluBI AGCT 4 cut(s) 615, 727, 1240, 1261
AluI AGCT 4 cut(s) 615, 727, 1240, 1261
Alw21I GWGCWC 4 cut(s) 617, 906, 1053, 1263
Alw26I GTCTC 3 cut(s) 410, 928, 1298
Alw44I GTGCAC 1 cut(s) 902
AlwI GGATC 4 cut(s) 568, 742, 755, 1265
Ama87I CYCGRG 1 cut(s) 103
AoxI GGCC 5 cut(s) 83, 410, 422, 511, 866
ApaLI GTGCAC 1 cut(s) 902
ApeKI GCWGC 3 cut(s) 251, 1005, 1355
ApoI RAATTY 1 cut(s) 671
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 7 cut(s) 83, 353, 410, 526, 551, 575, 866
AsuHPI GGTGA 4 cut(s) 325, 640, 711, 815
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 4 cut(s) 353, 526, 551, 575
AxyI CCTNAGG 1 cut(s) 1350
BaeGI GKGCMC 1 cut(s) 906
BalI TGGCCA 1 cut(s) 513
BamHI GGATCC 1 cut(s) 747
BanII GRGCYC 2 cut(s) 617, 1263
Bbv12I GWGCWC 4 cut(s) 617, 906, 1053, 1263
BbvCI CCTCAGC 2 cut(s) 1111, 1362
BbvI GCAGC 3 cut(s) 238, 992, 1342
BccI CCATC 5 cut(s) 224, 506, 671, 737, 1033
BceAI ACGGC 1 cut(s) 985
BcgI CGANNNNNNTGC 2 cut(s) 1006, 1040
BciT130I CCWGG 4 cut(s) 165, 366, 573, 1301
BciVI GTATCC 1 cut(s) 1021
BclI TGATCA 1 cut(s) 597
BcoDI GTCTC 3 cut(s) 410, 928, 1298
BcuI ACTAGT 2 cut(s) 370, 1120
BfaI CTAG 5 cut(s) 177, 371, 1064, 1121, 1384
BfmI CTRYAG 1 cut(s) 71
BfrI CTTAAG 1 cut(s) 637
BfuI GTATCC 1 cut(s) 1021
BglI GCCNNNNNGGC 1 cut(s) 409
BisI GCNGC 5 cut(s) 252, 401, 1006, 1356, 1359
BlpI GCTNAGC 1 cut(s) 728
BlsI GCNGC 5 cut(s) 253, 402, 1007, 1357, 1360
BmcAI AGTACT 3 cut(s) 693, 1076, 1119
Bme1390I CCNGG 4 cut(s) 165, 366, 573, 1301
Bme18I GGWCC 4 cut(s) 353, 526, 551, 575
BmeT110I CYCGRG 1 cut(s) 103
BmgBI CACGTC 1 cut(s) 420
BmgT120I GGNCC 7 cut(s) 83, 353, 410, 526, 551, 575, 866
BmiI GGNNCC 7 cut(s) 309, 496, 527, 552, 553, 749, 1347
BmrFI CCNGG 4 cut(s) 165, 366, 573, 1301
BmrI ACTGGG 1 cut(s) 837
BmsI GCATC 4 cut(s) 61, 387, 1026, 1085
BmuI ACTGGG 1 cut(s) 837
BplI GAGNNNNNCTC 4 cut(s) 97, 129, 1159, 1191
Bpu10I CCTNAGC 2 cut(s) 1111, 1362
Bpu1102I GCTNAGC 1 cut(s) 728
BsaAI YACGTR 1 cut(s) 956
BsaHI GRCGYC 2 cut(s) 972, 1172
BsaJI CCNNGG 2 cut(s) 163, 1203
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 6 cut(s) 407, 548, 875, 1090, 1253, 1281
Bse1I ACTGG 7 cut(s) 86, 445, 490, 496, 832, 1006, 1072
Bse21I CCTNAGG 1 cut(s) 1350
Bse3DI GCAATG 2 cut(s) 15, 514
BseBI CCWGG 4 cut(s) 165, 366, 573, 1301
BseDI CCNNGG 2 cut(s) 163, 1203
BseGI GGATG 5 cut(s) 52, 1017, 1032, 1100, 1273
BseLI CCNNNNNNNGG 6 cut(s) 407, 548, 875, 1090, 1253, 1281
BseMI GCAATG 2 cut(s) 15, 514
BseMII CTCAG 7 cut(s) 156, 926, 1125, 1242, 1353, 1362, 1364
BseNI ACTGG 7 cut(s) 86, 445, 490, 496, 832, 1006, 1072
BseRI GAGGAG 2 cut(s) 50, 105
BseSI GKGCMC 1 cut(s) 906
BseXI GCAGC 3 cut(s) 238, 992, 1342
BshFI GGCC 5 cut(s) 85, 412, 424, 513, 868
BsiHKAI GWGCWC 4 cut(s) 617, 906, 1053, 1263
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 2 cut(s) 404, 1014
BsiWI CGTACG 1 cut(s) 1158
BslFI GGGAC 4 cut(s) 537, 539, 1012, 1168
BslI CCNNNNNNNGG 6 cut(s) 407, 548, 875, 1090, 1253, 1281
BsmAI GTCTC 3 cut(s) 410, 928, 1298
BsmBI CGTCTC 2 cut(s) 410, 928
BsmFI GGGAC 4 cut(s) 537, 539, 1012, 1168
BsmI GAATGC 1 cut(s) 1291
BsnI GGCC 5 cut(s) 85, 412, 424, 513, 868
BsoBI CYCGRG 1 cut(s) 103
Bsp1286I GDGCHC 4 cut(s) 617, 906, 1053, 1263
Bsp143I GATC 6 cut(s) 78, 560, 597, 696, 747, 1270
Bsp1720I GCTNAGC 1 cut(s) 728
BspACI CCGC 5 cut(s) 401, 864, 940, 996, 1358
BspANI GGCC 5 cut(s) 85, 412, 424, 513, 868
BspCNI CTCAG 7 cut(s) 155, 925, 1124, 1241, 1354, 1363, 1363
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 7 cut(s) 309, 496, 527, 552, 553, 749, 1347
BspMAI CTGCAG 1 cut(s) 75
BspPI GGATC 4 cut(s) 568, 742, 755, 1265
BspTI CTTAAG 1 cut(s) 637
BsrDI GCAATG 2 cut(s) 15, 514
BsrI ACTGG 7 cut(s) 86, 445, 490, 496, 832, 1006, 1072
BssECI CCNNGG 2 cut(s) 163, 1203
BssMI GATC 6 cut(s) 78, 560, 597, 696, 747, 1270
BssNAI GTATAC 1 cut(s) 1377
BssNI GRCGYC 2 cut(s) 972, 1172
Bst1107I GTATAC 1 cut(s) 1377
Bst2UI CCWGG 4 cut(s) 165, 366, 573, 1301
Bst4CI ACNGT 4 cut(s) 653, 803, 937, 1204
Bst6I CTCTTC 1 cut(s) 1028
BstACI GRCGYC 2 cut(s) 972, 1172
BstAFI CTTAAG 1 cut(s) 637
BstBAI YACGTR 1 cut(s) 956
BstC8I GCNNGC 9 cut(s) 6, 362, 866, 902, 919, 1061, 1192, 1210, 1334
BstDEI CTNAG 8 cut(s) 142, 728, 912, 1111, 1228, 1350, 1362, 1371
BstDSI CCRYGG 1 cut(s) 1203
BstENI CCTNNNNNAGG 1 cut(s) 1088
BstF5I GGATG 5 cut(s) 52, 1017, 1032, 1100, 1273
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 6 cut(s) 81, 563, 600, 699, 750, 1273
BstMAI GTCTC 3 cut(s) 410, 928, 1298
BstMBI GATC 6 cut(s) 78, 560, 597, 696, 747, 1270
BstMWI GCNNNNNNNGC 3 cut(s) 409, 1002, 1014
BstNI CCWGG 4 cut(s) 165, 366, 573, 1301
BstNSI RCATGY 4 cut(s) 137, 921, 1194, 1212
BstSCI CCNGG 4 cut(s) 163, 364, 571, 1299
BstSFI CTRYAG 1 cut(s) 71
BstSLI GKGCMC 1 cut(s) 906
BstV1I GCAGC 3 cut(s) 238, 992, 1342
BstX2I RGATCY 1 cut(s) 747
BstXI CCANNNNNNTGG 1 cut(s) 420
BstYI RGATCY 1 cut(s) 747
BstZ17I GTATAC 1 cut(s) 1377
Bsu36I CCTNAGG 1 cut(s) 1350
BsuI GTATCC 1 cut(s) 1021
BsuRI GGCC 5 cut(s) 85, 412, 424, 513, 868
BtgI CCRYGG 1 cut(s) 1203
BtgZI GCGATG 1 cut(s) 608
BtrI CACGTC 1 cut(s) 420
BtsCI GGATG 5 cut(s) 52, 1017, 1032, 1100, 1273
BtsI GCAGTG 3 cut(s) 246, 566, 1201
BtsIMutI CAGTG 5 cut(s) 246, 452, 483, 566, 1201
Cac8I GCNNGC 9 cut(s) 6, 362, 866, 902, 919, 1061, 1192, 1210, 1334
CciI TCATGA 1 cut(s) 609
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 7 cut(s) 83, 353, 410, 526, 551, 575, 866
CseI GACGC 3 cut(s) 910, 961, 1180
Csp6I GTAC 5 cut(s) 489, 692, 1075, 1118, 1159
CspCI CAANNNNNGTGG 3 cut(s) 28, 503, 538
CviQI GTAC 5 cut(s) 489, 692, 1075, 1118, 1159
DdeI CTNAG 8 cut(s) 142, 728, 912, 1111, 1228, 1350, 1362, 1371
DpnI GATC 6 cut(s) 80, 562, 599, 698, 749, 1272
DpnII GATC 6 cut(s) 78, 560, 597, 696, 747, 1270
DrdI GACNNNNNNGTC 1 cut(s) 971
DseDI GACNNNNNNGTC 1 cut(s) 971
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 1028
EarI CTCTTC 1 cut(s) 1028
Ecl136II GAGCTC 2 cut(s) 615, 1261
Eco24I GRGCYC 2 cut(s) 617, 1263
Eco32I GATATC 1 cut(s) 25
Eco47I GGWCC 4 cut(s) 353, 526, 551, 575
Eco53kI GAGCTC 2 cut(s) 615, 1261
Eco57I CTGAAG 1 cut(s) 171
Eco81I CCTNAGG 1 cut(s) 1350
Eco88I CYCGRG 1 cut(s) 103
EcoICRI GAGCTC 2 cut(s) 615, 1261
EcoNI CCTNNNNNAGG 1 cut(s) 1088
EcoO109I RGGNCCY 2 cut(s) 526, 551
EcoRII CCWGG 4 cut(s) 163, 364, 571, 1299
EcoRV GATATC 1 cut(s) 25
EcoT38I GRGCYC 2 cut(s) 617, 1263
Esp3I CGTCTC 2 cut(s) 410, 928
FalI AAGNNNNNCTT 2 cut(s) 1224, 1256
FaqI GGGAC 4 cut(s) 537, 539, 1012, 1168
FauI CCCGC 2 cut(s) 857, 989
FbaI TGATCA 1 cut(s) 597
FblI GTMKAC 3 cut(s) 384, 1068, 1376
Fnu4HI GCNGC 5 cut(s) 252, 401, 1006, 1356, 1359
FokI GGATG 5 cut(s) 39, 1004, 1019, 1107, 1280
FriOI GRGCYC 2 cut(s) 617, 1263
Fsp4HI GCNGC 5 cut(s) 252, 401, 1006, 1356, 1359
FspBI CTAG 5 cut(s) 177, 371, 1064, 1121, 1384
GlaI GCGC 1 cut(s) 51
GluI GCNGC 5 cut(s) 252, 401, 1006, 1356, 1359
HaeIII GGCC 5 cut(s) 85, 412, 424, 513, 868
HapII CCGG 2 cut(s) 404, 1014
HgaI GACGC 3 cut(s) 910, 961, 1180
HhaI GCGC 1 cut(s) 52
Hin1I GRCGYC 2 cut(s) 972, 1172
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 8 cut(s) 290, 465, 642, 785, 892, 959, 978, 1296
HpaII CCGG 2 cut(s) 404, 1014
HphI GGTGA 4 cut(s) 325, 640, 711, 815
Hpy166II GTNNAC 4 cut(s) 385, 904, 1069, 1377
Hpy188I TCNGA 8 cut(s) 145, 190, 316, 602, 964, 977, 1231, 1372
Hpy188III TCNNGA 5 cut(s) 610, 700, 982, 1027, 1036
Hpy8I GTNNAC 4 cut(s) 385, 904, 1069, 1377
HpyAV CCTTC 7 cut(s) 10, 105, 135, 321, 497, 680, 1247
HpyCH4III ACNGT 4 cut(s) 653, 803, 937, 1204
HpyCH4IV ACGT 3 cut(s) 419, 955, 1157
HpyF10VI GCNNNNNNNGC 3 cut(s) 409, 1002, 1014
HpyF3I CTNAG 8 cut(s) 142, 728, 912, 1111, 1228, 1350, 1362, 1371
HpySE526I ACGT 3 cut(s) 419, 955, 1157
Hsp92I GRCGYC 2 cut(s) 972, 1172
HspAI GCGC 1 cut(s) 50
KflI GGGWCCC 1 cut(s) 551
Ksp22I TGATCA 1 cut(s) 597
Kzo9I GATC 6 cut(s) 78, 560, 597, 696, 747, 1270
LmnI GCTCC 2 cut(s) 97, 313
Lsp1109I GCAGC 3 cut(s) 238, 992, 1342
LweI GCATC 4 cut(s) 61, 387, 1026, 1085
MaeI CTAG 5 cut(s) 177, 371, 1064, 1121, 1384
MaeII ACGT 3 cut(s) 419, 955, 1157
MaeIII GTNAC 5 cut(s) 581, 856, 872, 1123, 1196
MalI GATC 6 cut(s) 80, 562, 599, 698, 749, 1272
MboI GATC 6 cut(s) 78, 560, 597, 696, 747, 1270
MboII GAAGA 5 cut(s) 32, 88, 177, 708, 1015
MflI RGATCY 1 cut(s) 747
MhlI GDGCHC 4 cut(s) 617, 906, 1053, 1263
MlsI TGGCCA 1 cut(s) 513
MluCI AATT 4 cut(s) 181, 342, 619, 671
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 3 cut(s) 459, 779, 901
MmeI TCCRAC 3 cut(s) 205, 951, 1262
Mox20I TGGCCA 1 cut(s) 513
MscI TGGCCA 1 cut(s) 513
MseI TTAA 2 cut(s) 638, 1133
MslI CAYNNNNRTG 1 cut(s) 1037
Msp20I TGGCCA 1 cut(s) 513
MspCI CTTAAG 1 cut(s) 637
MspI CCGG 2 cut(s) 404, 1014
MspR9I CCNGG 4 cut(s) 165, 366, 573, 1301
Mva1269I GAATGC 1 cut(s) 1291
MvaI CCWGG 4 cut(s) 165, 366, 573, 1301
MwoI GCNNNNNNNGC 3 cut(s) 409, 1002, 1014
NdeII GATC 6 cut(s) 78, 560, 597, 696, 747, 1270
NlaIV GGNNCC 7 cut(s) 309, 496, 527, 552, 553, 749, 1347
NmuCI GTSAC 5 cut(s) 581, 856, 872, 1123, 1196
NspI RCATGY 4 cut(s) 137, 921, 1194, 1212
PaeI GCATGC 3 cut(s) 921, 1194, 1212
PagI TCATGA 1 cut(s) 609
PciI ACATGT 1 cut(s) 133
PctI GAATGC 1 cut(s) 1291
PfeI GAWTC 5 cut(s) 290, 642, 959, 978, 1296
Pfl23II CGTACG 1 cut(s) 1158
PfoI TCCNGGA 1 cut(s) 1299
PkrI GCNGC 5 cut(s) 253, 402, 1007, 1357, 1360
PleI GAGTC 3 cut(s) 459, 779, 900
PpsI GAGTC 3 cut(s) 459, 779, 900
Ppu21I YACGTR 1 cut(s) 956
PpuMI RGGWCCY 2 cut(s) 526, 551
PscI ACATGT 1 cut(s) 133
Psp124BI GAGCTC 2 cut(s) 617, 1263
Psp5II RGGWCCY 2 cut(s) 526, 551
Psp6I CCWGG 4 cut(s) 163, 364, 571, 1299
PspGI CCWGG 4 cut(s) 163, 364, 571, 1299
PspLI CGTACG 1 cut(s) 1158
PspN4I GGNNCC 7 cut(s) 309, 496, 527, 552, 553, 749, 1347
PspPI GGNCC 7 cut(s) 83, 353, 410, 526, 551, 575, 866
PspPPI RGGWCCY 2 cut(s) 526, 551
PstI CTGCAG 1 cut(s) 75
PsuI RGATCY 1 cut(s) 747
RsaI GTAC 5 cut(s) 490, 693, 1076, 1119, 1160
RsaNI GTAC 5 cut(s) 489, 692, 1075, 1118, 1159
RseI CAYNNNNRTG 1 cut(s) 1037
SacI GAGCTC 2 cut(s) 617, 1263
SaqAI TTAA 2 cut(s) 638, 1133
SatI GCNGC 5 cut(s) 252, 401, 1006, 1356, 1359
Sau3AI GATC 6 cut(s) 78, 560, 597, 696, 747, 1270
Sau96I GGNCC 7 cut(s) 83, 353, 410, 526, 551, 575, 866
ScaI AGTACT 3 cut(s) 693, 1076, 1119
SchI GAGTC 3 cut(s) 459, 779, 901
ScrFI CCNGG 4 cut(s) 165, 366, 573, 1301
SduI GDGCHC 4 cut(s) 617, 906, 1053, 1263
SfaNI GCATC 4 cut(s) 61, 387, 1026, 1085
SfcI CTRYAG 1 cut(s) 71
SinI GGWCC 4 cut(s) 353, 526, 551, 575
SmiMI CAYNNNNRTG 1 cut(s) 1037
SmlI CTYRAG 1 cut(s) 637
SmoI CTYRAG 1 cut(s) 637
SpeI ACTAGT 2 cut(s) 370, 1120
SphI GCATGC 3 cut(s) 921, 1194, 1212
Sse9I AATT 4 cut(s) 181, 342, 619, 671
SsiI CCGC 5 cut(s) 401, 864, 940, 996, 1358
SspI AATATT 1 cut(s) 1219
SspMI CTAG 5 cut(s) 177, 371, 1064, 1121, 1384
SstI GAGCTC 2 cut(s) 617, 1263
StyD4I CCNGG 4 cut(s) 163, 364, 571, 1299
TaaI ACNGT 4 cut(s) 653, 803, 937, 1204
TaiI ACGT 3 cut(s) 422, 958, 1160
TaqI TCGA 4 cut(s) 501, 675, 699, 1026
TasI AATT 4 cut(s) 181, 342, 619, 671
TatI WGTACW 3 cut(s) 691, 1074, 1117
TauI GCSGC 2 cut(s) 403, 1361
TfiI GAWTC 5 cut(s) 290, 642, 959, 978, 1296
Tru1I TTAA 2 cut(s) 638, 1133
Tru9I TTAA 2 cut(s) 638, 1133
TscAI CASTG 5 cut(s) 253, 452, 490, 573, 1201
TseFI GTSAC 5 cut(s) 581, 856, 872, 1123, 1196
TseI GCWGC 3 cut(s) 251, 1005, 1355
Tsp45I GTSAC 5 cut(s) 581, 856, 872, 1123, 1196
TspDTI ATGAA 2 cut(s) 598, 683
TspRI CASTG 5 cut(s) 253, 452, 490, 573, 1201
Vha464I CTTAAG 1 cut(s) 637
VneI GTGCAC 1 cut(s) 902
VpaK11BI GGWCC 4 cut(s) 353, 526, 551, 575
XagI CCTNNNNNAGG 1 cut(s) 1088
XapI RAATTY 1 cut(s) 671
XceI RCATGY 4 cut(s) 137, 921, 1194, 1212
XmiI GTMKAC 3 cut(s) 384, 1068, 1376
XspI CTAG 5 cut(s) 177, 371, 1064, 1121, 1384
ZrmI AGTACT 3 cut(s) 693, 1076, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.