pycom13g13650

Major allergen Pru

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0019285
Physical Location & Seq
Reverse (-)
9691763 .. 9691978
216 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 216 bp
ATGGGTGTTTTCACATATGAATCCGAGTTCACCTCCGTCATTCCCCCTGCTAGGTTGTTCAATGCCTTTGTTCTTGATGCTGACAACCTCATACCCAAGATTGCACCACAAGCAGTGAAGAGCACTGAGATCCTTGAAGGAGATGGAGGAGTTGGAACCATTAAGAAAGAGAGAGATCGATCAACTTCGGTGAAGGTAGCACATACAGCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

72

Amino Acids

7.64

Weight (kDa)

5.68

Isoelectric Point (pI)

29.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 56 3.1e-06 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019285)

Species Orthologous Gene IDs
malus_domestica MD00G1140400.v1.1
pyrus_communis pycom11g27310 pycom13g13650
rosa_chinensis RchiOBHm_Chr4g0424001 RchiOBHm_Chr7g0212461
rosa_multiflora Rmu_co8041456.1_g000001
rosa_roxburghii Rroxscaffold_5G00354710
rosa_rugosa Rorug04G0192500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 124
AfiI CCNNNNNNNGG 1 cut(s) 51
AgsI TTSAA 2 cut(s) 61, 137
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw21I GWGCWC 1 cut(s) 125
AlwI GGATC 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 22, 202
Bbv12I GWGCWC 1 cut(s) 125
BccI CCATC 1 cut(s) 137
BfaI CTAG 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 157
BmsI GCATC 1 cut(s) 67
BplI GAGNNNNNCTC 2 cut(s) 17, 49
Bsa29I ATCGAT 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 213
Bsc4I CCNNNNNNNGG 1 cut(s) 51
BseCI ATCGAT 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 117
BseRI GAGGAG 1 cut(s) 162
BshVI ATCGAT 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 125
BslI CCNNNNNNNGG 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 125
Bsp143I GATC 3 cut(s) 129, 175, 179
BspCNI CTCAG 1 cut(s) 118
BspDI ATCGAT 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 157
BspPI GGATC 1 cut(s) 124
BspQI GCTCTTC 1 cut(s) 113
BssMI GATC 3 cut(s) 129, 175, 179
Bst6I CTCTTC 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 126
BstKTI GATC 3 cut(s) 132, 178, 182
BstMBI GATC 3 cut(s) 129, 175, 179
BstMWI GCNNNNNNNGC 2 cut(s) 110, 206
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 178
BsuTUI ATCGAT 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 2 cut(s) 120, 123
ClaI ATCGAT 1 cut(s) 178
CviJI RGCY 1 cut(s) 209
CviKI_1 RGCY 1 cut(s) 209
DdeI CTNAG 1 cut(s) 126
DpnI GATC 3 cut(s) 131, 177, 181
DpnII GATC 3 cut(s) 129, 175, 179
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
FaiI YATR 4 cut(s) 16, 18, 92, 204
FauNDI CATATG 1 cut(s) 16
FspBI CTAG 1 cut(s) 51
HinfI GANTC 1 cut(s) 20
HphI GGTGA 2 cut(s) 22, 202
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 1 cut(s) 74
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 2 cut(s) 131, 187
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 1 cut(s) 104
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 206
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 212
Kzo9I GATC 3 cut(s) 129, 175, 179
LguI GCTCTTC 1 cut(s) 113
LpnPI CCDG 1 cut(s) 60
LweI GCATC 1 cut(s) 67
MaeI CTAG 1 cut(s) 51
MaeII ACGT 1 cut(s) 212
MalI GATC 3 cut(s) 131, 177, 181
MboI GATC 3 cut(s) 129, 175, 179
MboII GAAGA 1 cut(s) 130
MflI RGATCY 1 cut(s) 129
MhlI GDGCHC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 3 cut(s) 43, 98, 140
MseI TTAA 1 cut(s) 162
MwoI GCNNNNNNNGC 2 cut(s) 110, 206
NdeI CATATG 1 cut(s) 16
NdeII GATC 3 cut(s) 129, 175, 179
NlaIV GGNNCC 1 cut(s) 157
PciSI GCTCTTC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 20
Ppu21I YACGTR 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 157
PsuI RGATCY 1 cut(s) 129
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 162
Sau3AI GATC 3 cut(s) 129, 175, 179
SduI GDGCHC 1 cut(s) 125
SetI ASST 6 cut(s) 35, 56, 90, 198, 211, 215
SfaNI GCATC 1 cut(s) 67
SgeI CNNG 7 cut(s) 37, 59, 63, 86, 109, 122, 146
SspMI CTAG 1 cut(s) 51
TaiI ACGT 1 cut(s) 215
TaqI TCGA 1 cut(s) 178
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 2 cut(s) 120, 130
TspDTI ATGAA 1 cut(s) 33
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 2 cut(s) 120, 130
XspI CTAG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.