pycom13g17250

negative regulation of Fas signaling pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0016813
Physical Location & Seq
Reverse (-)
13015046 .. 13017161
2116 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g17250.1

Sequence Viewer

Length: 726 bp
ATGGCTCTACCGTTCGGGAAGGGTGGTGACGTGGAGTCGGGGCAGAATGGGCAACTTTACCCGAATCAGATGGAGACCCCTCAGCTCCGATGGGCCTTCATTCGGAAGGTTTACATGATCGTCGCTTTTCAGCTGCTTCTCACCGTCGGTGTCGCCTCTGCCGTTGTGTCCATCCCCTCCATTCCCGGCTTCGTCAGATCCGTGTCCGGCACCGTCACGTTCGTTTTCATTATGGTGTCCACCATTGGAGTTGCGATTGGGTTGTACTTTTACCACAAGAAGCATCCGTGGAATTATGTGCTTCTTACTCTCTTTACGATTTTACTTGCGGTTACGGTGGGATTCACTTGTTCCTTCAAAGCAGGGAAGAGCGTTCTTCTAGCTGTGATTCTGACTGCTACGGTTGTGGTGGCCTTAACTCTATACACATTCTGGGCAGTGAAGAGAGGCGCGGACTTCAGCTTCCTCGGGCCATTTTTGTTCGCTGCAACCCTCATGCTTTTGATGTTTGGTCTCATCCAGATATTGTTGCCTCTGGGGCCGTTTTCAAGGATGGTGTATTCTGCAATTGGAGCTCTCCTGTGCTGTGCTTATATTGTGTACGACACTGACAATCTGATAAAGAGGCTCAGCTACGATGAATACATATTCGGTGCACTTTCCATATACATTGATATCGTCCAACTCTTCTTGTTTCTGCTCTCTTTGATCAGCGGTGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bax1-I

Protein Analysis

242

Amino Acids

26.48

Weight (kDa)

9.24

Isoelectric Point (pI)

25.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bax1-I PF01027 32 - 236 5.3e-44 Inhibitor of apoptosis-promoting Bax1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016813)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g01040
malus_domestica MD13G1198700.v1.1
prunus_persica Prupe.1G020400_v2.0.a1
pyrus_communis pycom13g17250
rosa_chinensis RchiOBHm_Chr4g0387621
rosa_laevigata RLG00000010106
rosa_roxburghii Rroxscaffold_5G00334300
rosa_rugosa Rorug03G0311500 Rorug03G0311600
rosa_samantha Rh4AG017200 Rh4BG012000 Rh4CG018400 Rh4DG013000
rosa_wichuraiana Rw4G001100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 209
AccII CGCG 1 cut(s) 452
AciI CCGC 3 cut(s) 329, 452, 714
AclWI GGATC 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 442
AfaI GTAC 2 cut(s) 266, 602
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 2 cut(s) 358, 549
AhdI GACNNNNNGTC 1 cut(s) 34
AjiI CACGTC 1 cut(s) 31
AluBI AGCT 6 cut(s) 85, 133, 383, 462, 575, 633
AluI AGCT 6 cut(s) 85, 133, 383, 462, 575, 633
Alw21I GWGCWC 2 cut(s) 577, 658
Alw26I GTCTC 2 cut(s) 68, 518
Alw44I GTGCAC 1 cut(s) 654
AlwI GGATC 1 cut(s) 192
Ama87I CYCGRG 1 cut(s) 467
AoxI GGCC 4 cut(s) 93, 411, 470, 539
ApaLI GTGCAC 1 cut(s) 654
ApeKI GCWGC 2 cut(s) 133, 485
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 3 cut(s) 93, 470, 539
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 2 cut(s) 38, 133
AvaI CYCGRG 1 cut(s) 467
BaeGI GKGCMC 1 cut(s) 658
BanI GGYRCC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 577
Bbv12I GWGCWC 2 cut(s) 577, 658
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 120, 472
BccI CCATC 4 cut(s) 64, 84, 179, 547
BceAI ACGGC 2 cut(s) 146, 526
BclI TGATCA 1 cut(s) 708
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 2 cut(s) 68, 518
BfaI CTAG 1 cut(s) 380
BglI GCCNNNNNGGC 1 cut(s) 538
BisI GCNGC 2 cut(s) 134, 486
BlpI GCTNAGC 1 cut(s) 629
BlsI GCNGC 2 cut(s) 135, 487
Bme1390I CCNGG 1 cut(s) 186
BmeRI GACNNNNNGTC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 467
BmgBI CACGTC 1 cut(s) 31
BmgT120I GGNCC 3 cut(s) 93, 470, 539
BmiI GGNNCC 2 cut(s) 211, 540
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 1 cut(s) 292
Bpu10I CCTNAGC 1 cut(s) 81
Bpu1102I GCTNAGC 1 cut(s) 629
BpuMI CCSGG 1 cut(s) 186
BsaI GGTCTC 2 cut(s) 68, 518
BsaJI CCNNGG 2 cut(s) 287, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 287, 466
BseGI GGATG 4 cut(s) 171, 283, 516, 558
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMII CTCAG 2 cut(s) 95, 643
BseSI GKGCMC 1 cut(s) 658
BseXI GCAGC 2 cut(s) 120, 472
Bsh1236I CGCG 1 cut(s) 452
BshFI GGCC 4 cut(s) 95, 413, 472, 541
BshNI GGYRCC 1 cut(s) 209
BsiHKAI GWGCWC 2 cut(s) 577, 658
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 2 cut(s) 186, 207
BslI CCNNNNNNNGG 1 cut(s) 102
BsmAI GTCTC 2 cut(s) 68, 518
BsnI GGCC 4 cut(s) 95, 413, 472, 541
Bso31I GGTCTC 2 cut(s) 68, 518
BsoBI CYCGRG 1 cut(s) 467
Bsp1286I GDGCHC 2 cut(s) 577, 658
Bsp143I GATC 3 cut(s) 117, 197, 708
Bsp1720I GCTNAGC 1 cut(s) 629
BspACI CCGC 3 cut(s) 329, 452, 714
BspANI GGCC 4 cut(s) 95, 413, 472, 541
BspCNI CTCAG 2 cut(s) 94, 642
BspFNI CGCG 1 cut(s) 452
BspLI GGNNCC 2 cut(s) 211, 540
BspPI GGATC 1 cut(s) 192
BspQI GCTCTTC 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 209
BspTNI GGTCTC 2 cut(s) 68, 518
BssECI CCNNGG 2 cut(s) 287, 466
BssMI GATC 3 cut(s) 117, 197, 708
Bst4CI ACNGT 5 cut(s) 12, 145, 214, 337, 403
Bst6I CTCTTC 3 cut(s) 362, 437, 692
BstDEI CTNAG 2 cut(s) 81, 629
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 4 cut(s) 171, 283, 516, 558
BstFNI CGCG 1 cut(s) 452
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 3 cut(s) 120, 200, 711
BstMAI GTCTC 2 cut(s) 68, 518
BstMBI GATC 3 cut(s) 117, 197, 708
BstMWI GCNNNNNNNGC 3 cut(s) 49, 538, 572
BstSCI CCNGG 1 cut(s) 184
BstSLI GKGCMC 1 cut(s) 658
BstUI CGCG 1 cut(s) 452
BstV1I GCAGC 2 cut(s) 120, 472
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BsuRI GGCC 4 cut(s) 95, 413, 472, 541
BtgI CCRYGG 1 cut(s) 287
BtrI CACGTC 1 cut(s) 31
BtsCI GGATG 4 cut(s) 171, 283, 516, 558
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 2 cut(s) 444, 606
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 3 cut(s) 93, 470, 539
Csp6I GTAC 2 cut(s) 265, 601
CviAII CATG 2 cut(s) 115, 496
CviQI GTAC 2 cut(s) 265, 601
DdeI CTNAG 2 cut(s) 81, 629
DpnI GATC 3 cut(s) 119, 199, 710
DpnII GATC 3 cut(s) 117, 197, 708
DriI GACNNNNNGTC 1 cut(s) 34
Eam1104I CTCTTC 3 cut(s) 362, 437, 692
Eam1105I GACNNNNNGTC 1 cut(s) 34
EarI CTCTTC 3 cut(s) 362, 437, 692
Ecl136II GAGCTC 1 cut(s) 575
Eco24I GRGCYC 1 cut(s) 577
Eco31I GGTCTC 2 cut(s) 68, 518
Eco32I GATATC 1 cut(s) 676
Eco53kI GAGCTC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 442
Eco88I CYCGRG 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 575
EcoRV GATATC 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 577
FaeI CATG 2 cut(s) 118, 499
FaiI YATR 9 cut(s) 116, 233, 297, 424, 497, 594, 647, 665, 667
FatI CATG 2 cut(s) 114, 495
FbaI TGATCA 1 cut(s) 708
Fnu4HI GCNGC 2 cut(s) 134, 486
FokI GGATG 4 cut(s) 158, 270, 503, 565
FriOI GRGCYC 1 cut(s) 577
Fsp4HI GCNGC 2 cut(s) 134, 486
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 451
GluI GCNGC 2 cut(s) 134, 486
HaeIII GGCC 4 cut(s) 95, 413, 472, 541
HapII CCGG 2 cut(s) 186, 207
HhaI GCGC 1 cut(s) 452
Hin1II CATG 2 cut(s) 118, 499
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HinfI GANTC 4 cut(s) 35, 64, 342, 388
HpaII CCGG 2 cut(s) 186, 207
HphI GGTGA 2 cut(s) 38, 133
Hpy166II GTNNAC 4 cut(s) 112, 240, 601, 656
Hpy188I TCNGA 6 cut(s) 69, 89, 105, 197, 393, 618
Hpy188III TCNNGA 2 cut(s) 16, 520
Hpy8I GTNNAC 4 cut(s) 112, 240, 601, 656
Hpy99I CGWCG 2 cut(s) 125, 149
HpyAV CCTTC 4 cut(s) 13, 100, 106, 364
HpyCH4III ACNGT 5 cut(s) 12, 145, 214, 337, 403
HpyCH4IV ACGT 2 cut(s) 30, 218
HpyCH4V TGCA 3 cut(s) 488, 566, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 538, 572
HpyF3I CTNAG 2 cut(s) 81, 629
HpySE526I ACGT 2 cut(s) 30, 218
Hsp92II CATG 2 cut(s) 118, 499
HspAI GCGC 1 cut(s) 450
Ksp22I TGATCA 1 cut(s) 708
Kzo9I GATC 3 cut(s) 117, 197, 708
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 2 cut(s) 90, 572
LpnPI CCDG 7 cut(s) 199, 220, 348, 418, 521, 533, 593
Lsp1109I GCAGC 2 cut(s) 120, 472
LweI GCATC 1 cut(s) 292
MaeI CTAG 1 cut(s) 380
MaeII ACGT 2 cut(s) 30, 218
MaeIII GTNAC 3 cut(s) 26, 214, 331
MalI GATC 3 cut(s) 119, 199, 710
MboI GATC 3 cut(s) 117, 197, 708
MboII GAAGA 4 cut(s) 368, 379, 454, 679
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 2 cut(s) 577, 658
MluCI AATT 2 cut(s) 292, 567
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 706
MnlI CCTC 8 cut(s) 90, 166, 187, 440, 476, 503, 543, 618
MseI TTAA 1 cut(s) 416
MslI CAYNNNNRTG 1 cut(s) 233
MspA1I CMGCKG 2 cut(s) 133, 714
MspI CCGG 2 cut(s) 186, 207
MspR9I CCNGG 1 cut(s) 186
MunI CAATTG 1 cut(s) 567
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 3 cut(s) 49, 538, 572
NciI CCSGG 1 cut(s) 186
NdeII GATC 3 cut(s) 117, 197, 708
NlaIII CATG 2 cut(s) 118, 499
NlaIV GGNNCC 2 cut(s) 211, 540
NmuCI GTSAC 2 cut(s) 26, 214
PciSI GCTCTTC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 198
PfeI GAWTC 3 cut(s) 64, 342, 388
PkrI GCNGC 2 cut(s) 135, 487
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Psp124BI GAGCTC 1 cut(s) 577
PspN4I GGNNCC 2 cut(s) 211, 540
PspPI GGNCC 3 cut(s) 93, 470, 539
PsuI RGATCY 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 133
RsaI GTAC 2 cut(s) 266, 602
RsaNI GTAC 2 cut(s) 265, 601
RseI CAYNNNNRTG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 362
SaqAI TTAA 1 cut(s) 416
SatI GCNGC 2 cut(s) 134, 486
Sau3AI GATC 3 cut(s) 117, 197, 708
Sau96I GGNCC 3 cut(s) 93, 470, 539
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 2 cut(s) 577, 658
SetI ASST 9 cut(s) 33, 87, 111, 135, 221, 385, 464, 577, 635
SfaNI GCATC 1 cut(s) 292
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 2 cut(s) 292, 567
SsiI CCGC 3 cut(s) 329, 452, 714
SspMI CTAG 1 cut(s) 380
SstI GAGCTC 1 cut(s) 577
StyD4I CCNGG 1 cut(s) 184
TaaI ACNGT 5 cut(s) 12, 145, 214, 337, 403
TaiI ACGT 2 cut(s) 33, 221
TasI AATT 2 cut(s) 292, 567
TatI WGTACW 1 cut(s) 264
TfiI GAWTC 3 cut(s) 64, 342, 388
Tru1I TTAA 1 cut(s) 416
Tru9I TTAA 1 cut(s) 416
TscAI CASTG 2 cut(s) 444, 613
TseFI GTSAC 2 cut(s) 26, 214
TseI GCWGC 2 cut(s) 133, 485
Tsp45I GTSAC 2 cut(s) 26, 214
TspDTI ATGAA 3 cut(s) 88, 217, 654
TspGWI ACGGA 2 cut(s) 190, 276
TspRI CASTG 2 cut(s) 444, 613
VneI GTGCAC 1 cut(s) 654
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.