Rh4DG013000

Belongs to the BI1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0016813
Physical Location & Seq
Reverse (-)
2058323 .. 2060069
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG013000.1

Sequence Viewer

Length: 720 bp
ATGGGTTTACCACAGTTCATGAAGAGTACCGACGTAGAGGCCCAAAAAAATGGCCAACTTTACCCTAATCAGATGGAGACGCCTCAGCTCCGCTGGGCCTTCATCCGCAAGGTTTACAGCATTATCTGCTGCCAGCTGGCTCTCACCGTCGCAGTCGCCGCCGTCGTGGTCACCGTCAAAGAGATTTCCAAGTTCTTCCATACGTTAGAAGGACTAATCACCTTCGTATGCCTCATTATCCTTACCGTAGCCATTGCTATTTCGTTGTGGTGGTTGCACAACAAGCATCCTTGGAATTATTTGCTGCTTATTTTGTTTACCTTTGCTGAGGCGATTGTGATCGGGGTCTGTTGTTCATATAAACAAGGGAAGGTTGTCATGCTGGCTGTGATTCTGACGCTGACGGTGGTTGTTGGCTTAACTGCATACACATTCTGGGCAGTCAAAAGAGGAGCGGATTTCGCCTTCCTTGGACCATTTTTATTCGCTGCATCGCTCATGCTTTTTACTTTTGCCCTCATTCAGATTTTCTTACCTATGGGGCCGTTATCGAAGACTATATTTGCAGCATTGTCAGGGATTCTGGCCTGTGCTTATATTGTATACGACACAGACAACATCATCAAGCGCCTGAGCTACGATGAGTACATCATGGGAGCTCTCAATATATATACCGATATTGTCATGCTGTTTCTGTCTATTCTACGTTTGCTCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bax1-I

Protein Analysis

239

Amino Acids

26.77

Weight (kDa)

8.7

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bax1-I PF01027 33 - 238 1.6e-43 Inhibitor of apoptosis-promoting Bax1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016813)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g01040
malus_domestica MD13G1198700.v1.1
prunus_persica Prupe.1G020400_v2.0.a1
pyrus_communis pycom13g17250
rosa_chinensis RchiOBHm_Chr4g0387621
rosa_laevigata RLG00000010106
rosa_roxburghii Rroxscaffold_5G00334300
rosa_rugosa Rorug03G0311500 Rorug03G0311600
rosa_samantha Rh4AG017200 Rh4BG012000 Rh4CG018400 Rh4DG013000
rosa_wichuraiana Rw4G001100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 455
AccI GTMKAC 1 cut(s) 603
AciI CCGC 4 cut(s) 91, 106, 159, 455
AcoI YGGCCR 1 cut(s) 52
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 2 cut(s) 28, 647
AluBI AGCT 4 cut(s) 88, 136, 636, 659
AluI AGCT 4 cut(s) 88, 136, 636, 659
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 1 cut(s) 71
AoxI GGCC 5 cut(s) 39, 52, 96, 542, 585
ApeKI GCWGC 4 cut(s) 129, 304, 488, 566
AspLEI GCGC 1 cut(s) 630
AspS9I GGNCC 4 cut(s) 40, 96, 473, 542
AsuHPI GGTGA 3 cut(s) 136, 163, 211
AvaII GGWCC 1 cut(s) 473
BalI TGGCCA 1 cut(s) 54
BanII GRGCYC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 560
Bbv12I GWGCWC 1 cut(s) 661
BbvCI CCTCAGC 2 cut(s) 84, 327
BbvI GCAGC 4 cut(s) 116, 291, 475, 578
BccI CCATC 1 cut(s) 67
BceAI ACGGC 2 cut(s) 146, 529
BcoDI GTCTC 1 cut(s) 71
BfoI RGCGCY 1 cut(s) 631
BisI GCNGC 5 cut(s) 130, 159, 305, 489, 567
BlsI GCNGC 5 cut(s) 131, 160, 306, 490, 568
Bme18I GGWCC 1 cut(s) 473
BmgT120I GGNCC 4 cut(s) 40, 96, 473, 542
BmiI GGNNCC 1 cut(s) 543
BmsI GCATC 2 cut(s) 295, 500
BpiI GAAGAC 1 cut(s) 560
Bpu10I CCTNAGC 3 cut(s) 84, 327, 632
BsaBI GATNNNNATC 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 290, 469
Bse3DI GCAATG 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 338
BseDI CCNNGG 2 cut(s) 290, 469
BseGI GGATG 2 cut(s) 102, 286
BseJI GATNNNNATC 1 cut(s) 338
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 3 cut(s) 98, 318, 623
BseRI GAGGAG 1 cut(s) 465
BseXI GCAGC 4 cut(s) 116, 291, 475, 578
BseYI CCCAGC 1 cut(s) 93
BshFI GGCC 5 cut(s) 41, 54, 98, 544, 587
BsiHKAI GWGCWC 1 cut(s) 661
BsmAI GTCTC 1 cut(s) 71
BsmBI CGTCTC 1 cut(s) 71
BsnI GGCC 5 cut(s) 41, 54, 98, 544, 587
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 1 cut(s) 339
BspACI CCGC 4 cut(s) 91, 106, 159, 455
BspANI GGCC 5 cut(s) 41, 54, 98, 544, 587
BspCNI CTCAG 3 cut(s) 97, 319, 624
BspHI TCATGA 1 cut(s) 18
BspLI GGNNCC 1 cut(s) 543
BsrBI CCGCTC 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 252
BssECI CCNNGG 2 cut(s) 290, 469
BssMI GATC 1 cut(s) 339
BssNAI GTATAC 1 cut(s) 604
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 2 cut(s) 290, 469
Bst1107I GTATAC 1 cut(s) 604
Bst4CI ACNGT 5 cut(s) 15, 148, 175, 247, 406
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 126
BstC8I GCNNGC 3 cut(s) 134, 138, 384
BstDEI CTNAG 3 cut(s) 84, 327, 632
BstEII GGTNACC 1 cut(s) 169
BstF5I GGATG 2 cut(s) 102, 286
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 1 cut(s) 342
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 1 cut(s) 339
BstMWI GCNNNNNNNGC 4 cut(s) 126, 158, 283, 461
BstPI GGTNACC 1 cut(s) 169
BstV1I GCAGC 4 cut(s) 116, 291, 475, 578
BstV2I GAAGAC 1 cut(s) 560
BstXI CCANNNNNNTGG 1 cut(s) 50
BstZ17I GTATAC 1 cut(s) 604
BsuRI GGCC 5 cut(s) 41, 54, 98, 544, 587
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 2 cut(s) 102, 286
Cac8I GCNNGC 3 cut(s) 134, 138, 384
CciI TCATGA 1 cut(s) 18
CfoI GCGC 1 cut(s) 630
Cfr13I GGNCC 4 cut(s) 40, 96, 473, 542
CseI GACGC 2 cut(s) 88, 406
Csp6I GTAC 2 cut(s) 27, 646
CviAII CATG 5 cut(s) 19, 379, 499, 652, 685
CviQI GTAC 2 cut(s) 27, 646
DdeI CTNAG 3 cut(s) 84, 327, 632
DpnI GATC 1 cut(s) 341
DpnII GATC 1 cut(s) 339
EaeI YGGCCR 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Ecl136II GAGCTC 1 cut(s) 659
Eco130I CCWWGG 2 cut(s) 290, 469
Eco24I GRGCYC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 473
Eco53kI GAGCTC 1 cut(s) 659
Eco91I GGTNACC 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 659
EcoO65I GGTNACC 1 cut(s) 169
EcoT14I CCWWGG 2 cut(s) 290, 469
EcoT38I GRGCYC 1 cut(s) 661
ErhI CCWWGG 2 cut(s) 290, 469
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 5 cut(s) 22, 382, 502, 655, 688
FatI CATG 5 cut(s) 18, 378, 498, 651, 684
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 5 cut(s) 130, 159, 305, 489, 567
FokI GGATG 2 cut(s) 89, 273
FriOI GRGCYC 1 cut(s) 661
Fsp4HI GCNGC 5 cut(s) 130, 159, 305, 489, 567
GlaI GCGC 1 cut(s) 629
GluI GCNGC 5 cut(s) 130, 159, 305, 489, 567
GsaI CCCAGC 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 631
HaeIII GGCC 5 cut(s) 41, 54, 98, 544, 587
HgaI GACGC 2 cut(s) 88, 406
HhaI GCGC 1 cut(s) 630
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 5 cut(s) 22, 382, 502, 655, 688
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HinfI GANTC 2 cut(s) 391, 580
HphI GGTGA 3 cut(s) 136, 163, 211
Hpy166II GTNNAC 4 cut(s) 8, 115, 318, 604
Hpy188I TCNGA 3 cut(s) 72, 396, 525
Hpy188III TCNNGA 1 cut(s) 19
Hpy8I GTNNAC 4 cut(s) 8, 115, 318, 604
Hpy99I CGWCG 3 cut(s) 35, 152, 167
HpyAV CCTTC 5 cut(s) 109, 203, 232, 364, 475
HpyCH4III ACNGT 5 cut(s) 15, 148, 175, 247, 406
HpyCH4IV ACGT 3 cut(s) 33, 203, 706
HpyCH4V TGCA 4 cut(s) 277, 425, 491, 566
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 158, 283, 461
HpyF3I CTNAG 3 cut(s) 84, 327, 632
HpySE526I ACGT 3 cut(s) 33, 203, 706
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 5 cut(s) 22, 382, 502, 655, 688
HspAI GCGC 1 cut(s) 628
Kzo9I GATC 1 cut(s) 339
LmnI GCTCC 3 cut(s) 93, 452, 656
LpnPI CCDG 9 cut(s) 79, 122, 146, 368, 421, 561, 569, 601, 644
Lsp1109I GCAGC 4 cut(s) 116, 291, 475, 578
LweI GCATC 2 cut(s) 295, 500
MaeII ACGT 3 cut(s) 33, 203, 706
MaeIII GTNAC 1 cut(s) 169
MalI GATC 1 cut(s) 341
MbiI CCGCTC 1 cut(s) 455
MboI GATC 1 cut(s) 339
MboII GAAGA 3 cut(s) 34, 187, 565
MhlI GDGCHC 1 cut(s) 661
MlsI TGGCCA 1 cut(s) 54
MluCI AATT 1 cut(s) 295
MluNI TGGCCA 1 cut(s) 54
MnlI CCTC 6 cut(s) 31, 93, 242, 322, 443, 527
Mox20I TGGCCA 1 cut(s) 54
MscI TGGCCA 1 cut(s) 54
MseI TTAA 2 cut(s) 419, 718
Msp20I TGGCCA 1 cut(s) 54
MspA1I CMGCKG 2 cut(s) 93, 136
MwoI GCNNNNNNNGC 4 cut(s) 126, 158, 283, 461
NdeII GATC 1 cut(s) 339
NlaIII CATG 5 cut(s) 22, 382, 502, 655, 688
NlaIV GGNNCC 1 cut(s) 543
NmuCI GTSAC 1 cut(s) 169
PagI TCATGA 1 cut(s) 18
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 171
PfeI GAWTC 2 cut(s) 391, 580
PkrI GCNGC 5 cut(s) 131, 160, 306, 490, 568
Psp124BI GAGCTC 1 cut(s) 661
PspEI GGTNACC 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 543
PspPI GGNCC 4 cut(s) 40, 96, 473, 542
PvuII CAGCTG 1 cut(s) 136
RsaI GTAC 2 cut(s) 28, 647
RsaNI GTAC 2 cut(s) 27, 646
SacI GAGCTC 1 cut(s) 661
SaqAI TTAA 2 cut(s) 419, 718
SatI GCNGC 5 cut(s) 130, 159, 305, 489, 567
Sau3AI GATC 1 cut(s) 339
Sau96I GGNCC 4 cut(s) 40, 96, 473, 542
SduI GDGCHC 1 cut(s) 661
SfaNI GCATC 2 cut(s) 295, 500
SinI GGWCC 1 cut(s) 473
Sse9I AATT 1 cut(s) 295
SsiI CCGC 4 cut(s) 91, 106, 159, 455
SstI GAGCTC 1 cut(s) 661
StyI CCWWGG 2 cut(s) 290, 469
TaaI ACNGT 5 cut(s) 15, 148, 175, 247, 406
TaiI ACGT 3 cut(s) 36, 206, 709
TaqI TCGA 1 cut(s) 551
TasI AATT 1 cut(s) 295
TatI WGTACW 1 cut(s) 645
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 2 cut(s) 391, 580
Tru1I TTAA 2 cut(s) 419, 718
Tru9I TTAA 2 cut(s) 419, 718
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 4 cut(s) 129, 304, 488, 566
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 4 cut(s) 7, 35, 91, 345
VpaK11BI GGWCC 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.